scholarly journals EFFECT OF CONIOTHYRIUM MINITANS AND TRICHODERMA HARZIANUM IN THE BIOLOGICAL CONTROL OF WHITE MOLD DISEASE (SCLEROTINIA SCLEROTIORUM) IN LETTUCE (LACTUCA SATIVA L.)

2019 ◽  
Vol 17 (6) ◽  
Author(s):  
A ÇOLAK ATEŞ
Keyword(s):  
Biological Control ◽  
Sclerotinia Sclerotiorum ◽  
Lactuca Sativa ◽  
Trichoderma Harzianum ◽  
White Mold ◽  
Coniothyrium Minitans ◽  
Lactuca Sativa L

scholarly journals Biological control of white mold by Trichoderma harzianum in common bean under field conditions

2015 ◽  
Vol 50 (12) ◽  
pp. 1220-1224 ◽  
Author(s):  
Daniel Diego Costa Carvalho ◽  
Alaerson Maia Geraldine ◽  
Murillo Lobo Junior ◽  
Sueli Corrêa Marques de Mello
Keyword(s):  
Biological Control ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Trichoderma Harzianum ◽  
Experimental Period ◽  
Field Conditions ◽  
White Mold ◽  
Control Treatment

Abstract: The objective of this work was to evaluate Trichoderma harzianum isolates for biological control of white mold in common bean (Phaseolus vulgaris). Five isolates were evaluated for biocontrol of white mold in 'Perola' common bean under field conditions, in the 2009 and 2010 crop seasons. A commercial isolate (1306) and a control treatment were included. Foliar applications at 2x109 conidia mL-1 were performed at 42 and 52 days after sowing (DAS), in 2009, and at 52 DAS in 2010. The CEN287, CEN316, and 1306 isolates decreased the number of Sclerotinia sclerotiorum apothecia per square meter in comparison to the control, in both crop seasons. CEN287, CEN316, and 1306 decreased white mold severity during the experimental period, when compared to the control.

Efficacy of Antagonists on mycelium growth and carpogenic germination of sclerotia of Sclerotinia sclerotiorum on Indian mustard

2016 ◽  
Vol 1 (02) ◽  
pp. 190-193
Author(s):  
Jhilmil Gupta
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Trichoderma Harzianum ◽  
Plant Pathogens ◽  
Foliar Spray ◽  
Indian Mustard ◽  
Plant Diseases ◽  
Stem Rot ◽  
Pseudomonas Chlororaphis ◽  
Coniothyrium Minitans ◽  
Carpogenic Germination

Sclerotinia sclerotiorum (Lib.) de Bary is a soil borne pathogen capable of infecting more than 400 host plants worldwide. It is a major pathogen that plays a crucial role in reducing the yield in economically important crops. The capability of sclerotia to survive for more than 4 years becomes very difficult to manage the crop from the infection of Sclerotinia rot fungus. Stem rot of indian mustard [Brassica juncea (L.) Czern and Cross] caused by Sclerotinia sclerotiorum is potentially a serious threat in many mustard growing areas in India. Treatments of seeds and foliar spray with of fungicides applied at regular intervals are effective in reducing infection, but uses of chemicals are hazardous, harmful for beneficial micro-organisms. Biological control of plant pathogens offers an exciting opportunity to manage plant diseases. In the present study, the efficacy of four bio-agents, viz., Coniothyrium minitans, Aspergilus nidulans, Trichoderma harzianum, and Pseudomonas chlororaphis were evaluated for the control of stem rot of Indian mustard. Results on bio-efficacy of different bioagents, when evaluated under glass house condition, the Coniothyrium minitans was the most effective agent and caused highest reduction (64.7 %) in carpogenic germination of sclerotia followed by Aspergilus nidulans (52.5 %) and Trichoderma harzianum (48.8 %), over control while Pseudomonas chlororaphis (48.3 %) was at par with T. harzianum. All the bioagents showed significant reduction effective in controlling the disease. Similar results were achieved when bioagents tested on dual inoculated plates.

Bacillus sp. FSQ1: a Promising Biological Control Agent Against Sclerotinia sclerotiorum, the Causal Agent of white Mold in Common Bean (Phaseolus vulgaris L.)

2021 ◽  
Vol 48 (6) ◽  
pp. 729-739
Author(s):  
María Fernanda Villarreal-Delgado ◽  
Fannie Isela Parra-Cota ◽  
Luis Alberto Cira-Chávez ◽  
María Isabel Estrada-Alvarado ◽  
Sergio de los Santos-Villalobos
Keyword(s):  
Biological Control ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Causal Agent ◽  
White Mold ◽  
Bacillus Sp ◽  
Phaseolus Vulgaris L

scholarly journals New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum

2016 ◽  
Vol 16 (3) ◽  
Author(s):  
Eder Marques ◽  
Irene Martins ◽  
Mariana de Oliveira Cardoso Cunha ◽  
Marcello Arrais Lima ◽  
João Batista Tavares da Silva ◽  
...  
Keyword(s):  
Biological Control ◽  
Information System ◽  
Genetic Resources ◽  
Sclerotinia Sclerotiorum ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
Complete Inhibition ◽  
Its Sequences ◽  
White Mold

Abstract Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored in the Embrapa Fungi Collection for Biological Control and the information obtained in the experiments will be incorporated into the database of biological assets within the genetic resources information system (Allele) and be made available for further studies.

Evaluation of filamentous fungi isolated from petals of bean and rapeseed for suppression of white mold

1992 ◽  
Vol 38 (2) ◽  
pp. 124-129 ◽  
Author(s):  
G. D. Inglis ◽  
G. J. Boland
Keyword(s):  
Biological Control ◽  
Filamentous Fungi ◽  
Fusarium Graminearum ◽  
Sclerotinia Sclerotiorum ◽  
Field Test ◽  
Active Ingredient ◽  
White Mold ◽  
Combination Treatments ◽  
Growth Room ◽  
Fusarium Heterosporum

The influence of filamentous fungi isolated from petals of bean and rapeseed on white mold caused by Sclerotinia sclerotiorum was evaluated. In laboratory trials, macerates of agar plugs containing hyphal fragments of the pathogen in combination with individual fungi were applied onto celery petioles, and subsequent lesion diameters were recorded. The efficacy of 10 fungi exhibiting a spectrum of lesion suppression on celery was correlated with the efficacy of the same fungi in growth-room (r = 0.78, P = 0.005) and greenhouse (r = 0.68, P = 0.032) trials. From 315 isolates of fungi evaluated in the laboratory trials, the 10 most suppressive isolates were selected and evaluated in growth-room trials. Spores of the test fungi were applied onto flowers simultaneously with, and up to 24 h after, inoculation with ascospores of S. sclerotiorum. The most suppressive fungi included isolates of Alternaria alternata, Drechslera sp., Epicoccum purpurascens, Fusarium graminearum, Fusarium heterosporum, and Myrothecium verrucaria. These fungi did not provide consistent control of white mold of bean in a field test repeated four times in 1988. However, Drechslera sp. and E. purpurascens significantly reduced the incidence of white mold in one and two trials, respectively. Combination treatments of A. alternata and Benlate (1.1 kg active ingredient ha−1) suppressed white mold significantly more than either treatment alone in one of four trials. Key words: Sclerotinia sclerotiorum, biological control, fungicide interaction.

scholarly journals Mecanismo de acción de cinco microorganismos promotores de crecimiento vegetal

2017 ◽  
Vol 34 (1) ◽  
pp. 17 ◽  
Author(s):  
Harold González F. ◽  
Natalia Fuentes M.
Keyword(s):  
Oryza Sativa ◽  
Pisum Sativum ◽  
Lactuca Sativa ◽  
Trichoderma Harzianum ◽  
Manihot Esculenta ◽  
Carica Papaya ◽  
Enterobacter Aerogenes ◽  
Bacillus Mycoides ◽  
Lactuca Sativa L ◽  
Pisum Sativum L

El uso indiscriminado de agroquímicos para aumentar la productividad de los cultivos, es una práctica no amigable con el ambiente. Como estrategia para desplazar el uso de químicos agrícolas, se aplica la bioinoculación de microorganismos promotores de crecimiento vegetal. En este contexto, el objetivo fue evaluar el efecto promotor del crecimiento vegetal de cinco cepas de microorganismos sobre seis especies de plantas. Se seleccionó un hongo (Trichoderma harzianum) y un grupo de bacterias (Enterobacter aerogenes, Azotobacter sp., Bacillus mycoides y Microbacterium sp.) para evaluar su efecto sobre la germinación, crecimiento y asimilación de plantas de yuca Manihot esculenta, arroz Oryza sativa, girasol Helianthus annuus L., arveja Pisum sativum L., lechuga Lactuca sativa L. y papaya Carica papaya L. Para el montaje del bioensayo se dispuso de 18 macetas para cada planta. Cada tratamiento se realizó por triplicado incluyendo el control. Se elaboró el inoculo sembrando una alícuota de cada microorganismos en 200mL de caldo nutritivo, y de este se agregaron 20mL a cada maceta por tratamiento. Se evaluaron las siguientes variables: número y longitud de raíces, hojas y tallo, área foliar y biomasa raíz y tallo. Los biopreparados a partir de Microbacterium sp. y B. mycoides, aceleraron la germinación de las plantas sin importar la especie. Los tratamientos de Azotobacter sp., T. harzianum y E. aerogenes no fueron efectivos para las plantas de yuca y arroz. La bioinoculación de Microbacterium sp., B. mycoides y E. aerogenes favoreció el crecimiento, desarrollo y rendimiento de las plantas de arroz y arveja.

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