scholarly journals Mononuclear subsets and cytokine profile of venous and capillary blood in patients with psoriasis and healthy people

2021 ◽  
Vol 23 (6) ◽  
pp. 1333-1346
Author(s):  
S. V. Sennikova ◽  
A. P. Toptygina ◽  
E. L. Semikina ◽  
R. Sh. Zakirov ◽  
S. S. Akulova
Keyword(s):  
Mononuclear Cells ◽  
Venous Blood ◽  
Clinical Analysis ◽  
Capillary Blood ◽  
Cytokine Profile ◽  
Healthy People ◽  
Local Immunity ◽  
Blood Samples ◽  
Healthy Group ◽  
Window Method

Psoriasis is considered an autoimmune disease with a predominantly cellular mechanism for the development of disorder. Studies in immune pathogenesis of psoriasis were performed either in animal model, which is not just similar to humans, or the data were obtained in patients by means of skin window method, which is traumatic, or by examining venous blood. However, it is difficult to discern parameters of the local immune response in venous blood samples. We have attempted to find an adequate method which would be convenient both for the patient and for the researcher, in order to assess local immune processes occurring in the skin affected by psoriasis. We examined 20 patients with a verified diagnosis of psoriasis, the average age was 44.3 years. The control group included 15 healthy adults, with average age of 46.6 years. Capillary blood was taken by fingerprick, whereas, in psoriatic patients, the samples were taken near the psoriatic lesion at a final volume of 400 μL in two microvettes. Venous blood (3 mL) was taken from the cubital vein into a vacuum tube with EDTA. Clinical analysis of venous and capillary blood was performed in automated hematological analyzer. Immunophenotyping was performed by four-color staining of whole capillary and venous blood followed by lysis of erythrocytes. Cytofluorometry was performed using techniques and reagents from BD Biosciences (USA). Plasma cytokines were determined by multiplex approach (MagPix, BioRad, USA). Upon clinical analysis of blood, the difference between capillary and venous blood was not found, either in healthy group, or among patients with psoriasis. In healthy people, the subsets of mononuclear cells, did not differ between venous and capillary blood. The samples of capillary and venous blood in the patients with psoriasis showed significantly increased levels of double-positive lymphocytes (CD45RA+/CD45R0+), B lymphocytes and NKT lymphocytes (both for relative and and absolute values). A significant increase in the percentage of naive T lymphocytes, activated helpers (Thact) and Treg, as well as B1 cells and Breg, and a significant decrease in B2 lymphocytes was registered in capillary blood of the patients with psoriasis. In venous blood samples from psoriatic patients, only a significant increase in Thact, Treg, and Breg was revealed. In the capillary blood of patients with psoriasis, we found a significant increase in the levels of non-classical M2 monocytes and inflammatory Minfl monocytes, and a decrease in classical M1 monocyte levels; in venous blood of psoriatic patients, only an increase in inflammatory Minfl monocytes was revealed. In capillary blood, all the studied cytokines in psoriasis patients significantly exceeded the levels of corresponding cytokines in healthy controls, except of IL-10. The levels of this cytokine did not differ from healthy group. In venous blood, the levels of most studied cytokines in the group of patients with psoriasis did not differ from the group of healthy ones. Approximately two-fold increase was revealed for IL-4, IL-21, IL-23 and TNF. First, the subsets of mononuclear cells and the cytokine profile of capillary and venous blood of healthy people did not differ significantly. Secondly, our proposed method for determining the subsets of mononuclear cells and capillary blood cytokines profile from the area of psoriatic lesions may be used to monitor local immunity in the patients with psoriasis. This approach is significantly less traumatic than the skin window method and more informative than the studies of venous blood.

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

scholarly journals Blood Glucose Assay Using Dextrostix and a Reflectance Meter

1972 ◽  
Vol 9 (1-6) ◽  
pp. 91-94 ◽  
Author(s):  
I. W. Percy-Robb ◽  
R. S. McMaster ◽  
A. D. B. Harrower ◽  
L. J. P. Duncan
Keyword(s):  
Blood Glucose ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Blood Samples ◽  
Laboratory Staff ◽  
Glucose Assay ◽  
Reflectance Meter ◽  
High Degree ◽  
Blood Glucose Concentrations

The ‘Dextrostix’-reflectance meter system for blood glucose analysis has been evaluated using a blood glucose reference method. A high degree of concordance between the two methods was obtained when analyses were performed by skilled laboratory staff on venous blood samples containing fluoride, with a 75 s contact time. Skilled laboratory staff performed significantly better than unskilled staff. Capillary blood glucose concentrations correlated poorly with concentrations in venous blood samples taken at the same time as the capillary blood.

scholarly journals Use of Capillary Blood Samples Leads to Higher Parasitemia Estimates and Higher Diagnostic Sensitivity of Microscopic and Molecular Diagnostics of Malaria Than Venous Blood Samples

2018 ◽  
Vol 218 (8) ◽  
pp. 1296-1305 ◽  
Author(s):  
Johannes Mischlinger ◽  
Paul Pitzinger ◽  
Luzia Veletzky ◽  
Mirjam Groger ◽  
Rella Zoleko-Manego ◽  
...  
Keyword(s):  
Molecular Diagnostics ◽  
Venous Blood ◽  
Capillary Blood ◽  
Diagnostic Sensitivity ◽  
Blood Samples

Comparison of capillary blood versus venous blood samples in the assessment of immunity to measles

1996 ◽  
Vol 61 (1-2) ◽  
pp. 73-77 ◽  
Author(s):  
F. Novello ◽  
B. Ridolfi ◽  
L. Fiore ◽  
G. Buttinelli ◽  
E. Medda ◽  
...  
Keyword(s):  
Venous Blood ◽  
Capillary Blood ◽  
Blood Samples

Accuracy and precision of analyses for total cholesterol as measured with the Reflotron cholesterol method.

1989 ◽  
Vol 35 (8) ◽  
pp. 1734-1739 ◽  
Author(s):  
P S Bachorik ◽  
R H Bradford ◽  
T Cole ◽  
I Frantz ◽  
A M Gotto ◽  
...  
Keyword(s):  
Plasma Cholesterol ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Average Difference ◽  
Blood Samples ◽  
Laboratory Values ◽  
Accuracy And Precision ◽  
Single Finger ◽  
Made In

Abstract We compared plasma cholesterol measurements made with the Boehringer Mannheim Reflotron reflectance photometric analyzer in 1298 capillary blood samples with measurements made in venous blood samples collected at the same time and analyzed in four standardized Lipid Research Clinics laboratories. The Reflotron measurements averaged 0.8% to 7.8% lower than the laboratory values. Correlations (r) between the two sets of measurements ranged from 0.92 to 0.96. In some samples, however, the Reflotron values differed from the laboratory values by greater than or equal to 12%; the cholesterol concentrations in these samples tended to be higher than in those for which better agreement was observed. The smaller negative biases were observed when test strips were used that were calibrated with reference to the Centers for Disease Control Reference Method for cholesterol. The agreement between sequential Reflotron values averaged less than or equal to 4.3%. There was an average difference of less than or equal to 1.0% between Reflotron measurements made in each of two sequential capillary blood samples taken from a single finger puncture.

An Assessment of Dried Blood-Spot Technology for Identifying Iron Deficiency

Blood ◽  
1998 ◽  
Vol 92 (5) ◽  
pp. 1807-1813 ◽  
Author(s):  
James D. Cook ◽  
Carol H. Flowers ◽  
Barry S. Skikne
Keyword(s):  
Iron Deficiency ◽  
Transferrin Receptor ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Epidemiologic Studies ◽  
Capillary Blood ◽  
Deficiency Anemia ◽  
Blood Samples ◽  
Blood Spots ◽  
American Society

Abstract The present study was undertaken to assess the feasibility of using ferritin and transferrin receptor measurements on dried capillary blood spots to identify iron deficiency (ID) in public health surveys. Measurements on serum and blood spots prepared from venous blood were performed in 71 healthy subjects, 41 of whom were iron-replete and 30 who had ID, either without (n = 20) or with (n = 10) anemia. Parallel measurements were performed on hemolyzed whole blood and washed hemolyzed red blood cells to assess the erythrocyte contribution of ferritin and transferrin receptor to dried blood samples. The concentration of ferritin in dried blood samples was threefold higher than serum assays due to the release of ferritin from hemolyzed erythrocytes, which diminished the usefulness of ferritin measurements for detecting ID. On the other hand, there was negligible erythrocyte contribution to the measurement of transferrin receptor in dried blood spots. The most sensitive parameter in dried blood spots was the ratio of receptor/ferritin, which was suitable for identifying iron-deficiency anemia (IDA), but less reliable than serum assays for detecting milder ID without anemia. We conclude that tandem measurements of serum ferritin and transferrin receptor in dried blood spots can be used to facilitate the identification of IDA in epidemiologic studies. © 1998 by The American Society of Hematology.

scholarly journals Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples

2020 ◽  
Author(s):  
Margaret A Black ◽  
Guomiao Shen ◽  
Xiaojun Feng ◽  
Wilfredo Garcia Beltran ◽  
Yang Feng ◽  
...  
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Venous Blood ◽  
Capillary Blood ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Immunoassay ◽  
Blood Samples ◽  
Significant Difference ◽  
Finger Stick

Objectives: Numerous serologic immunoassays have been launched to detect antibodies to SARS-CoV-2, including rapid tests. Here, we validate use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample. Methods: Samples collected by venous blood draw and capillary finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients negative for SARS-CoV-2. Samples were tested with the 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) lateral flow immunoassay, and antibody calls were compared with results obtained by ELISA. Results: The Biolidics LFI kit shows clinical sensitivity of 92% at 7 days after PCR diagnosis of SARS-CoV-2 on venous blood. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p=1.00), except for detection of IgM at D7 (p=0.04). Finger stick whole blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection. Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold) is comparable to ELISA and showed consistent results across different sample types. Furthermore, we show that capillary blood obtained by finger stick shows similar sensitivity for detecting anti-SARS-CoV-2 IgM and IgG antibodies as venous blood samples. This provides an opportunity for decentralized rapid testing in the community and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.

scholarly journals Ergogenic effect of pre-exercise chicken broth ingestion on a high-intensity cycling time-trial

2021 ◽  
Author(s):  
Silvia Barbaresi ◽  
Laura Blancquaert ◽  
Zoran Nikolovski ◽  
Sarah de Jager ◽  
Mathew Wilson ◽  
...  
Keyword(s):  
High Intensity ◽  
Venous Blood ◽  
Time Trial ◽  
Capillary Blood ◽  
Blood Analysis ◽  
Meat Extract ◽  
Blood Samples ◽  
Cycling Time Trial ◽  
Chicken Broth ◽  
Cycling Time

Abstract Background: chicken meat extract is a popular functional food in Asia. It is rich in the bioactive compounds carnosine and anserine, two histidine-containing dipeptides (HCD). Studies suggest that acute pre-exercise ingestion of chicken extracts has important applications towards exercise performance and fatigue control, but the evidence is equivocal. This study aimed to evaluate the ergogenic potential of the pre-exercise ingestion of a homemade chicken broth (CB) vs a placebo soup on a short-lasting, high-intensity cycling exercise. Methods: fourteen men participated in this double-blind, placebo-controlled, crossover intervention study. Subjects ingested either CB, thereby receiving 46.4 mg/kg body weight of HCD, or a placebo soup (similar in taste without HCD) 40 min before an 8 min cycling time trial (TT) was performed. Venous blood samples were collected at arrival (fasted), before exercise and at 5 min recovery. Plasma HCD were measured with UPLC-MS/MS and glutathione (in red blood cells) was measured through HPLC. Capillary blood samples were collected at different timepoints before and after exercise. Results: a significant improvement (p=0.033; 5.2%) of the 8 min TT mean power was observed after CB supplementation compared to placebo. Post-exercise plasma carnosine (p<0.05) and anserine (p<0.001) was significantly increased after CB supplementation and not following placebo. No significant effect of CB supplementation was observed either on blood glutathione levels, nor on capillary blood analysis. Conclusions: oral CB supplementation improved the 8 min TT performance albeit it did not affect the acid-base balance or oxidative status parameters. Further research should unravel the potential role and mechanisms of HCD, present in CB, in this ergogenic approach.

Comparison of Glucose Values of Blood Samples Taken in Three Different Ways

2017 ◽  
Vol 28 (4) ◽  
pp. 436-455 ◽  
Author(s):  
Tulay Sagkal Midilli ◽  
Eda Ergın ◽  
Ebru Baysal ◽  
Zeki Arı
Keyword(s):  
Blood Glucose ◽  
Venous Blood ◽  
Middle Finger ◽  
Blood Glucose Measurement ◽  
Capillary Blood ◽  
Glucose Measurement ◽  
Capillary Blood Glucose ◽  
Blood Samples ◽  
Left Hand ◽  
The Right

The purpose of the study was to determine differences and correlations between the blood glucose values of venous blood and the first and second drops of capillary blood samples taken in three different ways. Blood samples were (a) venous blood, (b) the first and second drops of capillary blood from the middle finger of the right hand (only washed with soap and water), and (c) the first and second drops of capillary blood from the middle finger of the left hand (washed with soap and water and cleaned with alcohol). It was concluded that the fasting capillary blood glucose values could be used in place of venous blood glucose values, that only washing the hands with neutral soap and water for 30 s could be sufficient for capillary blood glucose measurement, and that the first or second blood drop from a clean hand could be used for capillary blood glucose measurement.

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