scholarly journals R-ISSR - Tool for Generation of a New Type of Products, Applied for the Identification of Putative Molecular Marker Linked to QTL Determined Tolerance to Nutrient Deprivation Stress in Rye (Secale cereale L.)

2012 ◽  
Vol 40 (2) ◽  
pp. 238 ◽  
Author(s):  
Milosz SMOLIK
Keyword(s):  
Genetic Similarity ◽  
Plant Material ◽  
Inbred Lines ◽  
Mantel Test ◽  
Nutrient Deprivation ◽  
Secale Cereale L ◽  
New Type ◽  
Similar Range ◽  
Rapd And Issr ◽  
Issr Primers

This study presents results confirming the possibility of the application of various combinations of RAPD and ISSR primers in one multiplex PCR for the rye genome and generating a new type of R-ISSR products. The plant material consisted of two inbred lines (Ot0-6 and Ot1-3), F1 and two bulks (tolerant and susceptible), selected from a population of RILs (F9) with different responses to stress caused by nutrient deprivation at the seedling stage. In one PCR reaction, the DNA of five genotypes was amplified separately, with RAPD, ISSR, and RAPD+ISSR primers. In total, 28 R-ISSR combinations were tested by combining 20 and 8 appropriately selected RAPD and ISSR primers. 567 loci were amplified, including 230 RAPD, 136 ISSR and 207 amplified R-ISSR loci, of which 114 were new. It was shown that only one ISSR product was identified amongst sequenced R-ISSR products with identical electrophoretic mobility as co-migrating RAPDs and ISSRs. The remaining ones had heteroamplicons (R-ISSR) sequences. A similar range of variability was observed both in R-ISSR profiles and in RAPD, as well as in ISSR. The correlation coefficient between the matrices of genetic similarity for five rye genotypes calculated by means of the Mantel test was highly significant rAB.C = 0.964.

scholarly journals RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

2014 ◽  
Vol 6 (3) ◽  
pp. 292-299
Author(s):  
Marcelina KRUPA-MAŁKIEWICZ ◽  
Miłosz Smolik ◽  
Anna BARNIAK ◽  
Beata SMOLIK
Keyword(s):  
Genetic Variability ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Mantel Test ◽  
North West ◽  
The North ◽  
West Part ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.

THE GENETICS OF FERTILITY RESTORATION IN CYTOPLASMIC MALE-STERILE RYE

1979 ◽  
Vol 21 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. J. Scoles ◽  
L. E. Evans
Keyword(s):  
Secale Cereale ◽  
Fertility Restoration ◽  
Inbred Lines ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile ◽  
Restorer Genes ◽  
Secale Cereale L ◽  
Partial Fertility

Three inbred lines of rye (Secale cereale L.) known to be capable of restoring fertility to a cytoplasmic male-sterile line were crossed with the sterile line. The proportions of male fertile, partially male fertile and male sterile plants in F2 and backcross progenies indicated that three dominant restorer genes were present in each line. These were designated Rf1, Rf2 and Rf3, their relative expressivity was Rf1>Rf2>Rf3. Expressivity was dependent upon environment. Partial fertility occurred when certain genotypes carried two of the three alleles as dominant, but was dependent upon genotype and environment.

The use of random amplified microsatellite polymorphic DNA and coefficients of parentage to determine genetic relationships in barley

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 477-486 ◽  
Author(s):  
J A Dávila ◽  
M P Sánchez de la Hoz ◽  
Y Loarce ◽  
E Ferrer
Keyword(s):  
Hordeum Vulgare ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Spring Barley ◽  
Genetic Distances ◽  
Mantel Test ◽  
Winter Barley ◽  
Mean Values ◽  
Simple Sequence ◽  
Hordeum Vulgare Ssp

Seventy European barley lines (Hordeum vulgare ssp. vulgare) and 29 Hordeum vulgare ssp. spontaneum accessions were evaluated for random amplified microsatellite polymorphism (RAMP). PCR was performed with 5'-anchored primers complementary to microsatellites in combination with random primers. Of 20 primers assayed in barley, only 9 produced well-resolved fragment patterns in H. vulgare ssp. spontaneum. On the basis of 56 polymorphic fragments, genetic distances between the two subspecies were calculated. Barley samples were subdivided according to growth habit and spike morphology. The smallest genetic distance was found between winter cultivars and accessions of H. vulgare ssp. spontaneum. The 20 primers assayed in the barley lines produced 140 polymorphic fragments that were used to calculate genetic similarity between lines. Mean genetic similarity within groups of lines ranged from 0.693 for 6-rowed winter barley to 0.657 for 6-rowed spring barley. Within these groups, mean values were significantly higher than mean genetic similarity between groups. Principal-coordinate analysis clearly separated the 2-rowed spring and 6-rowed winter types. Cluster analysis of spring and winter types showed subclustering consistent with the available pedigrees. Coefficients of parentage (COPs) were calculated for 29 spring and 20 winter lines. Mean values of 0.0741 for spring barley and 0.0458 for winter barley were obtained. RAMP-based genetic similarity matrices were compared with the corresponding COP-based matrices by the Mantel test. The correlation between them was poor (r = 0.21, P < 0.05), indicating little relationship between these two estimators of genetic similarity. The relative influence of factors involved in the calculation of these two estimators is discussed.Key words: genetic diversity, microsatellites, simple sequence repeats, fingerprinting, barley.

scholarly journals Phylogenetic relationships between members of Amaranthaceae and Chenopodiaceae of lower gangetic plains using RAPD and ISSR markers

1970 ◽  
Vol 36 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Tui Ray ◽  
SC Roy
Keyword(s):  
Key Words ◽  
Phylogenetic Relationships ◽  
Issr Markers ◽  
Mantel Test ◽  
Microsatellite Repeat ◽  
Arbitrary Primers ◽  
Gangetic Plains ◽  
First Report ◽  
Rapd And Issr ◽  
Similarity Matrices

The present study is the first report of phylogenetic relationships between some economically important members of Amaranthaceae and Chenopodiaceae of Lower Gangetic Plains of India, using RAPD and ISSR markers. A total of 293 RAPD and 177 ISSR fragments were generated with 15 (out of 50) decamer arbitrary primers and 11 (out of 30) microsatellite repeat anchored primers, respectively. Mean genetic similarities were estimated at 0.47 and 0.45 using RAPDs and ISSRs, respectively. The members of Chenopodiaceae are separated from those of Amaranthaceae in both the cases. The mantel test between the two Jaccard's similarity matrices gave r = 0.83, showing good correlation between RAPD and ISSR based similarities. However, in both the dendrograms Celosia sp. showed distant relationships with other amaranths. Key words: Amaranthaceae, Chenopodiaceae, RAPD, ISSR, Phylogenetic relationships   doi:10.3329/bjb.v36i1.1544 Bangladesh J. Bot. 36(1): 21-28, 2007 (June)

Genetic diversity and relationships among Egyptian Galium (Rubiaceae) and related species using ISSR and RAPD markers

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Kadry Abdel Khalik ◽  
Magdy Abd El-Twab ◽  
Rasha Galal
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
Randomly Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeats ◽  
Close Relationship ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

AbstractGenetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.

RAPD polymorphism in the prebreeding material for cultivation of synthetic variations of lucerne (Medicago sativa L.)

2013 ◽  
Vol 8 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Lidia Skuza ◽  
Stanisława Rogalska ◽  
Stanisław Dyba ◽  
Jan Bocianowski
Keyword(s):  
Medicago Sativa ◽  
Genetic Similarity ◽  
Seed Set ◽  
Inbred Lines ◽  
Specific Yield ◽  
Group Method ◽  
Green Mass ◽  
Molecular Image ◽  
Synthetic Population ◽  
Medicago Sativa L

AbstractGenetic diversity between synthetic cultivars (Syn5, Syn7), inbred lines (D3, D5, E1/2, G1/1, G1/2) and ecotypes (E16, E51, E182, E231) of lucerne (Medicago sativa L.) was studied using the RAPD-PCR method. The plants differed in the efficiency of seed set and in the yield of green mass. The ecotypes E182 and E231 and the synthetic population Syn5 showed the highest fertility. Additionally, Syn5 also showed the highest efficiency of seed set and the yield of green mass. Among the inbred lines, D3 was characterised by the highest yield of green mass and E1/2 by the highest fertility. An optimal combination of yield and biomass was observed for the synthetic population Syn5, obtained by crossing the lines D3, D5 and G1/1, as demonstrated using comparative analysis. A total of 338 polymorphic products were generated using 20 arbitrary primers. Cluster analysis using the Unweighted-Pair Group Method with Arithmetic Mean (UPGMA) in the Molecular image Gel Doc™ XR (Bio-Rad) software based on the Dice’s coefficient of genetic similarity showed a division of the studied forms into two groups based on genetic similarity. The ecotype E16 formed one of the groups whereas all of the other ecotypes observed in this study clustered into the second group. A high level of polymorphism among the studied lucerne forms was detected indicating an interesting gene pool awaiting future exploration. Analysis of variance also supported a high diversity among the studied forms. This study provides insightful information into the heterosis effect of synthetic populations or hybrids of F1 lucerine by providing correlations between the genetic background of the inbred lines and their ability to produce a specific yield.

B-chromosomes in inbred lines of rye (Secale cereale L.)

Genetica ◽  
1994 ◽  
Vol 92 (3) ◽  
pp. 149-154 ◽  
Author(s):  
M. M. Jim�nez ◽  
E. Romera ◽  
M. J. Puertas ◽  
R. N. Jones
Keyword(s):  
Secale Cereale ◽  
Inbred Lines ◽  
B Chromosomes ◽  
Secale Cereale L

The effect of rye genotype on wheat–rye crossability and on the development of F1 seed

1983 ◽  
Vol 25 (6) ◽  
pp. 668-670 ◽  
Author(s):  
Graham J. Scoles
Keyword(s):  
Triticum Aestivum ◽  
Secale Cereale ◽  
Seed Set ◽  
Triticum Aestivum L ◽  
Inbred Lines ◽  
Hybrid Seed ◽  
Secale Cereale L

Five inbred lines of rye (Secale cereale L.) and an open-pollinated rye cultivar were used to pollinate wheat (Triticum aestivum L.) cultivars of differeing crossability. No hybrid seed was produced with the cultivar of low crossability, but with the highly crossable cultivar an average seed set of 65% was obtained. Significant differences in terms of seed set and weight of F1 seed were detected among the inbreds.

scholarly journals Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)

2014 ◽  
Vol 4 (3) ◽  
pp. 393-403
Author(s):  
J. S. DUHAN ◽  
S. Kajla ◽  
D. Choudhary ◽  
A. K. Poonia
Keyword(s):  
Tissue Culture ◽  
Genetic Stability ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Ms Medium ◽  
Inter Simple Sequence Repeats ◽  
Rapd Primer ◽  
Molecular Assessment ◽  
Rapd And Issr ◽  
Issr Primers

The investigation was carried out to assess the genetic stability in   tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers.Aims:  Molecular assessment of genetic stability of tissue culture raised plants of banana cv. G-9 using molecular markers.Material and Results: Apical shoots were established on medium EM4 (MS + BAP 4.0 mg L-1) with maximum of 3.8 buds/explant in 2.6 days. The maximum bud multiplication with 16.5±0.06 shoots was observed on medium Ma3 (MS medium+ 5.0 mg L-1 BAP + 0.25 mg L-1 NAA of + 30 mg L-1 AdSO4). The maximum rooting response (100%) was observed on 1/2 MS medium supplemented with 2.0 mg L-1 NAA in 12.2 days. After acclimatization the hardened plants were examined for genetic stability using RAPD and ISSR primers. Total forty six (twenty six RAPD and twenty ISSR) markers were used. RAPD primers produced 87 distinct and scorable bands, with an average of 3.34 bands per primer and the amplification products range was from 100-1200 bps. The number of scorable bands for RAPD primer varied from 2 to 5 with an average of 3.34 bands per primer. ISSR primers produced 71 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 6 with an average of 3.55 bands per primer.Conclusion: Similar profile with monomorphic bands was observed for all the tissue culture raised plants when compared to mother plant in both types of markers used. The results corroborate the fact that plant tissue culture technology has immense importance for production of true to type of planting material. 

scholarly journals Cytogenetic and molecular studies on two faba bean cultivars revealed their difference in their aluminum tolerance

2020 ◽  
Vol 116 (2) ◽  
pp. 273
Author(s):  
Ahmed M. HASSANEIN ◽  
Ahmed H. MOHAMED ◽  
Heba Ahmed ABD ALLAH ◽  
Hoida ZAKI
Keyword(s):  
Faba Bean ◽  
Chromosomal Abnormalities ◽  
Aluminum Tolerance ◽  
Issr Markers ◽  
Growth Data ◽  
Al Tolerance ◽  
Al Stress ◽  
Molecular Studies ◽  
Rapd And Issr ◽  
Issr Primers

<p>Two cultivars of faba bean (<em>Vicia faba</em> ‘Giza 843’ and ‘Nobaria 3’) that differ in aluminum (Al) tolerance were used to study cytogenetic and genomic alterations under the influence of Al Cl<sub>3</sub> (5, 15, and 25 mmol AlCl<sub>3</sub>) for different periods (6, 12 and 24 h). Under Al treatments, mitotic index in both cultivars decreased and total chromosomal abnormalities increased. The frequencies of micronuclei and chromosomal abnormalities (C-anaphase, metaphase-star chromosomes, breaks, sticky and disturbed chromosomes during metaphase or anaphase) in ‘Giza 843’ were lower than in ‘Nabaria 3’. Increase of the registered cytogenetic events under the influence of Al stress led to increase the detected polymorphism using RAPD and ISSR markers. Application of RAPD primers gave the same value of polymorphism in both faba bean cultivars under Al stress. Polymorphism average of nine ISSR primers of ’Giza 843’ (65.36 %) was lower than that of ‘Nobaria 3’ (71.59 %). Molecular markers, cytogenetic characteristics and seedling growth data indicate that Al tolerance of ‘Giza 843’ was higher than of ‘Nobaria 3’. This work shows that cytogenetic and ISSR techniques could be used efficiently to distinguish between the ability of two faba bean cultivars to tolerate toxic effects of Al.</p>

Sign in / Sign up

Export Citation Format

Share Document