scholarly journals Preliminary screening of selected soils in Ilorin, North-central Nigeria for antibiotic-producing actinomycetes

2020 ◽  
Vol 12 (1) ◽  
pp. 143-153
Author(s):  
Risikat N. AHMED ◽  
Sesan M. SOBA ◽  
Mercy O. BAMIGBOYE ◽  
Kamoldeen A. AJIJOLAKEWU

The present research aimed at screening various soils within Ilorin metropolis for antibiotic producing actinomycetes. The objectives of the study were to determine physiochemical parameters of soils, the occurrence of actinomycetes in soils, antibacterial potentials and identity of isolates. Soil parameters such as pH, temperature, moisture, organic matter and soil type were evaluated following standard procedures. Selective isolation to determine the occurrence of actinomycetes was performed by soil dilution using pour plate technique on starch casein agar. Preliminary antibacterial screening against 10 clinical test bacteria was performed using cross streak method. All isolates were initially identified based on morphological and biochemical characteristics, while the most bioactive isolates were further identified by molecular means. The soils were alkaline, with temperatures between 29 °C and 31 °C, moisture was in range of 0.72 ± 0.07c and 6.62 ± 0.42b. Highest organic matter content was 32.13 ± 0.20a with soil types mostly loamy and sandy loam. Ten actinomycetes (SM1 - SM10) were isolated, with the most frequently occurring isolate being SM3 and SM5 (16.7%). SM5 was the most active, inhibiting 9 out of 10 tests, with the highest inhibition against Staphylococcus aureus 25923 (24 mm ± 0.15a). All isolates were identified as Streptomyces by morphology and biochemical tests. Based on nucleotide similarity searches and phylogeny, two bioactive Streptomycetes were suggested as novel strains and thus named as Streptomyces bottropensis UIL RNA (SM5) and S. flavoviridis UIL RNA(SM7), which may serve as promising sources of antibiotics. Actinomycetes from Ilorin metropolis demonstrated broad spectrum of antibacterial activity against clinical test bacteria.

1990 ◽  
Vol 4 (2) ◽  
pp. 279-283 ◽  
Author(s):  
Michael R. Blumhorst ◽  
Jerome B. Weber ◽  
Len R. Swain

Field experiments were conducted on six loam and sandy loam soils to study the influence of various soil parameters on atrazine, cyanazine, alachlor, metolachlor, and pendimethalin efficacy. Herbicidal activity was highly correlated to the soil organic content. Humic matter content was equally or better correlated (r = 0.70 to 0.91) with herbicide bioactivity than was organic matter content (r = 0.66 to 0.84). Regression equations were determined which allow herbicide rate recommendations for 80% weed control to be calculated based on soil humic matter or organic matter levels.


1976 ◽  
Vol 56 (3) ◽  
pp. 129-138 ◽  
Author(s):  
A. J. MACLEAN

The Cd concentration in 10 plant species grown in a neutral surface soil (0.65 ppm Cd) varied from 0.18 ppm in potato tubers to 0.99 ppm in soybean roots on a dry matter basis. Addition of 5 ppm Cd increased the concentrations in the plants markedly and they were particularly high in lettuce (10.36 ppm) and tobacco leaves (11.57 ppm). Cd concentrations tended to be lower in the edible portion (seed, fruit, tubers) than in other plant parts. Added Cd affected yields in only a few instances. But in another experiment, Cd added at a rate of 5 ppm to five soils decreased the yield of lettuce in most instances. In a comparison of results for two similarly managed sandy loam soils, nearly neutral in reaction but differing in organic matter content (2.17 vs. 15.95% organic C), the concentration of Cd was lower in lettuce grown in the soil with the higher amount of organic matter. The Cd content of the lettuce was reduced by liming some of the acid soils. Addition of Cd increased the concentration of Zn in the plants appreciably, but added Zn did not affect Cd uptake. In an incubation experiment comprising five soils, DTPA (diethylenetriamine-pentaacetic acid) extractable Cd decreased with liming of three Cd-treated acid soil samples. In comparisons of two sandy loam soils and of surface and subsoil layers of a sand, extractable Cd increased with higher amounts of soil organic matter.


2019 ◽  
Vol 78 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Beata Bosiacka ◽  
Helena Więcław ◽  
Paweł Marciniuk ◽  
Marek Podlasiński

Abstract The vegetation of protected salt meadows along the Baltic coast is fairly well known; however, dandelions have been so far treated as a collective species. The aim of our study was to examine the microspecies diversity of the genus Taraxacum in Polish salt and brackish coastal meadows and to analyse soil property preferences of the dandelion microspecies identified. In addition, we analysed the relations between soil properties and vegetation patterns in dandelion-supporting coastal meadows (by canonical correspondence analysis). The salt and brackish meadows along the Polish Baltic coast we visited were found to support a total of 27 dandelion microspecies representing 5 sections. Analysis of vegetation patterns showed all the soil parameters (C:N ratio, organic matter content, pH, concentration of Mg, P, K, electrolytic conductivity of the saturated soil extract ECe) to explain 32.07% of the total variance in the species data. The maximum abundance of most dandelion microspecies was associated with the highest soil fertility, moderate pH values and organic matter content, and with the lowest magnesium content and soil salinity. The exceptions were T. latissimum, T. stenoglossum, T. pulchrifolium and T. lucidum the occur-rence of which was related to the lowest soil fertility and the highest salinity. In addition, several microspecies (T. leptodon, T. gentile, T. haematicum, T. fusciflorum and T. balticum) were observed at moderate C:N ratios and ECe. Four other microspecies (T. infestum, T. cordatum, T. hamatum, T. sertatum) occurred at the lowest pH and organic matter content. The information obtained increases the still insufficient body of knowledge on ecological spectra of individual dandelion microspecies, hence their potential indicator properties.


1982 ◽  
Vol 62 (1) ◽  
pp. 165-175 ◽  
Author(s):  
C. R. DE KIMPE ◽  
M. BERNIER-CARDOU ◽  
P. JOLICOEUR

Twenty-one topsoils, with texture varying from sandy loam to clay and organic matter content ranging from 1.6 to 11.9%, were submitted to compaction and settling at different moisture contents where dry bulk density was determined. Under compaction, the density curve went through a maximum while a minimum was observed in the case of settling. Optimum moisture contents corresponding to these two characteristic densities were almost the same. The most important physical properties affecting soil behavior under compaction and settling were found to be water retention properties at low matric potential which themselves depended primarily on organic matter content. Samples submitted to compaction had saturated hydraulic conductivities less than 1 cm/h, while after settling, Ksat measurements ranged from 0.8 to 234 cm/h. Organic matter played an important role in reducing the effects of compaction, and moisture content alone was not sufficient to predict the best conditions for workability in the fields.


Soil Research ◽  
2016 ◽  
Vol 54 (7) ◽  
pp. 880
Author(s):  
Mohammad Reza Chaichi ◽  
Marcus Turcios ◽  
Mina Rostamza

Non-ionic surfactants have been well researched as a tool to ameliorate water repellent conditions. However, few studies have evaluated the risks and benefits of non-ionic surfactant applications in wettable soil. The objective of this study was to evaluate the effects of a surfactant in modifying the wetting pattern in soils of different textures and organic matter contents. The experimental treatments consisted of (1) four different soil textures including sandy, sandy loam, sandy clay loam and silt loam, (2) four different organic matter contents (0.2, 0.7, 1.2 and 1.7% by weight), and (3) irrigation water treatments with or without surfactant (IrrigAid Gold). The experiment was carried out in Plexiglas boxes with one drip emitter under the soil surface. The results demonstrated the superiority of surfactant application on increasing water distribution in the soil profile for all soil textural classes. Silt loam texture had the highest side wetted area and wetting depth 45min after the initiation of irrigation. Upward capillary water movement and top wetted area significantly decreased in the surfactant treatment across all soil textures except in sandy soil. As organic matter content increased, top wetted area decreased. These findings clarified the potential ability of surfactant in increasing water infiltration in non-repellent soil in an in vitro system.


2021 ◽  
Author(s):  
Vito Abbruzzese

In many farm systems, both inorganic and organic fertilisers, including manure and slurry, are applied to the soil to replenish nutrient offtake in agricultural products and additional nutrient losses to soil as well as surface water and groundwater. With respect to sole reliance on inorganic fertilisers, the use of manure/slurry as a nutrient resource offers important benefits, including the reuse and recycling of nitrogen (N) and phosphorus (P) within farming systems as well as a reduction in the reliance on agricultural production on finite inorganic fertiliser reserves. There is increasing interest in the extent to which additives can enhance the nutrient value of slurry/manure. However, little is known about the impacts of these amended slurries/manures on the quantity and composition of N and P within agricultural and pasture soils. We report data from incubation experiments in which soils received a range of treatments, including the application of livestock slurry that had received a mixture of commercial additives. Our experiments were designed to understand how slurry that has received additives ultimately affects nutrient availability in organic, clay-loam and sandy-loam grassland soils. The addition of the additives to slurry resulted in a slight increase or no difference in total solids, pH, total N, ammonium-N, total P, total potassium, total magnesium and total sodium compared to the untreated counterpart. We considered the effects of our treatments on a range of agronomically important soil parameters, including Olsen-P, mineral-N, available-K, pH and organic matter content. This experiment aimed to understand the extent to which soil fertility could be enhanced through the application of slurries/manures that have received additives. The application of both amended and unamended slurry treatments on soil led to higher values of NH4-N, available-K, available Mg and pH than the addition of inorganic fertiliser. In addition, no substantial differences were observed between the treatment of the three soils with unamended and amended slurry.


1961 ◽  
Vol 7 (4) ◽  
pp. 507-513 ◽  
Author(s):  
E. Strzelczyk

This study represents an attempt to correlate the low numbers of Azotobacter in rhizosphere and root-free soils at the Central Experimental Farm, Ottawa, with the incidence of bacterial and actinomycete antagonists of this organism. Wheat, radish, and onion were grown in the greenhouse in two soils varying greatly in fertility and organic matter content, and isolations of bacteria and actinomycetes were made periodically for testing against Azotobacter. It was found that rhizosphere soil contained greater numbers of microorganisms antagonistic to Azotobacter than root-free soil. Of the three crops used wheat exerted the least effect. In all the tests numbers of antagonists were greater in the fertile Granby sandy loam than in the infertile Upland sand. The results correlated well with the Azotobacter populations in these soils as reported in the first paper of this series.


Parasitology ◽  
2017 ◽  
Vol 144 (14) ◽  
pp. 1956-1963 ◽  
Author(s):  
APOSTOLOS KAPRANAS ◽  
ABIGAIL M. D. MAHER ◽  
CHRISTINE T. GRIFFIN

SUMMARYIn laboratory experiments, we investigated how media with varying ratio of peat:sand and two levels of compaction influence dispersal success of entomopathogenic nematode (EPN) species with different foraging strategies: Steinernema carpocapsae (ambusher), Heterorhabditis downesi (cruiser) and Steinernema feltiae (intermediate). Success was measured by the numbers of nematodes moving through a 4 cm column and invading a wax moth larva. We found that both compaction and increasing peat content generally decreased EPN infective juvenile (IJ) success for all three species. Of the three species, H. downesi was the least affected by peat content, and S. carpocapsae was the most adversely influenced by compaction. In addition, sex ratios of the invading IJs of the two Steinernema species were differentially influenced by peat content, and in the case of S. feltiae, sex ratio was also affected by compaction. This indicates that dispersal of male and female IJs is differentially affected by soil parameters and that this differentiation is species-specific. In conclusion, our study shows that organic matter: sand ratio and soil compaction have a marked influence on EPN foraging behaviour with implications for harnessing them as biological pest control agents.


Weed Science ◽  
1999 ◽  
Vol 47 (3) ◽  
pp. 349-352 ◽  
Author(s):  
Chris H. Tingle ◽  
David R. Shaw ◽  
Patrick D. Gerard

Laboratory studies were conducted to evaluate14C-flumetsulam mobility in two Mississippi soils of varied texture and organic matter content following delays in irrigation. Mobility was evaluated using packed soil columns, 25 cm deep, under unsaturated–saturated flow conditions. Irrigation timings included 0, 3, and 5 d after flumetsulam application. Flumetsulam mobility (defined as the amount collected in leachate) decreased from 45% to no more than 20% of the applied in the Prentiss sandy loam soil when irrigation was delayed 3 or 5 d. With the Okolona soil, flumetsulam recovery in the leachate was 21, 14, and 6%, respectively when irrigation occurred 0, 3, and 5 d after application. Flumetsulam proved to be mobile when irrigation immediately followed application, with 6 to 45% recovered in the leachate from all soils evaluated. The Prentiss soil retained 6% of the applied flumetsulam in the upper 5 cm and the Okolona soil retained 22% when irrigation immediately followed flumetsulam application. When the irrigation interval was delayed at least 3 d, the Okolona soil retained 40% in the upper 5 cm, whereas the Prentiss soil retained 10%. Flumetsulam mobility was dependent on irrigation timing and soil type.


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