scholarly journals Interaction Effects of Arbuscular Mycorrhizal Fungi and Different Phosphate Levels on Growth Performance of Catharanthus roseus Linn.

2011 ◽  
Vol 3 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Mohd AYOOB ◽  
Irfan AZIZ ◽  
Paramjit Kaur JITE

Catharanthus roseus L. (Apocynaceae), a valuable medicinal plant with potential therapeutic value was inoculated with AM fungi Glomus fasciculatum under three different phosphate conditions. Catharanthus roseus plants raised in presence of the AM fungi showed increased growth in terms of (shoot length, root length, leaf number, fresh weight and dry weight). Total chlorophyll content and phosphate content of the shoot was found to be significantly higher in AM inoculated plants as compared to non AM Catharanthus plants. The activities of phosphatase enzymes were found to be increased in AM inoculated plants as compared to non AM plants. Root colonization percent was significantly higher in AM inoculated plants at zero and at all three phosphate levels after 60, 90 and 120 days of AM inoculation, but decreased at third phosphate level after 120 days of AM inoculation. The study suggests that Catharanthus roseus is dependent on the mycorrhizal fungi to a large extent for its growth and survival and also shows the potential of AM fungi Glomus fasciculatum in increasing growth and biomass of Catharanthus roseus L.

2016 ◽  
Vol 73 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
Nampiah SUKARNO ◽  
Latifah Kosim DARUSMAN ◽  
Didiek Hadjar GOENADI ◽  
Sally SMITH ◽  
...  

SummaryA green house experiment was conducted tostudy the effect of spore number and species ofAM fungi as inoculant of oil palm. Two species ofAM fungi was evaluated in this study namelyAcaulospora tuberculata and Gigaspora margaritaand three spore number were tested i. e 200, 350,and 500 spores. There two fungi have thepotential as AM fungi inoculant for oil palm. Thesoil used was acid soil from Cikopomayak, WestJava while the oil palm seedling was from OilPalm Research Institute, Medan. A polybag sized20 x 40 cm was used. Spores as type of inoculantaffect the oil palm growth in longer time. Thebest growth of the seedling in term of height,fresh, and dry weight was obtained byinoculation at 500 spores of A. tuberculata andG. margarita. However, at 500 spores perpolybag, growth and N, P, and K uptake ofseedlings inoculated with A. tuberculata andG. margarita were not significantly differentexcept for seedling and root fresh weight. Oilpalm seedling inoculated with A. tuberculata at500 spores per seedling resulted higher root andseedling fresh weight compared with thoseinoculated with G. margarita. The different effectof seedling on A. tuberculata and G. margaritainoculation at 200 and 350 spores per seedlingwere only observed in plant height, fresh and dryweight of seedlings. The plant height, fresh, anddry weight of seedlings inoculated withA. tuberculata at 200 and 350 spores per seedlingwere higher compared with those inoculatedwith G. margarita. In addition inoculation withA. tuberculata at 200 spores per seedling resultedhigher N and K uptake of seedling compared withthose inoculated with G. margarita.RingkasanSuatu penelitian rumah kaca telah dilakukanuntuk mempelajari pengaruh jumlah spora danspesies cendawan mikoriza arbuskula (CMA)sebagai inokulum pada bibit kelapa sawit. Duaspesies CMA yang diuji ialah Acaulosporatuberculata dan Gigaspora margarita sedangkanjumlah spora yang diuji ada tiga tingkat yaitu200, 350, dan 500 spora. Bibit kelapa sawitberumur dua bulan ditanam di polibag berukuran20 x 40 cm yang berisi tanah yang bereaksimasam berasal dari Cikopomayak. Hasil yangdiperoleh menunjukkan bahwa spora sebaganokulum bibit kelapa sawit dapat mempengaruhipertumbuhan kelapa sawit namun diperlukanwaktu yang lebih lama untuk mendapatkanrespons inokulasi. Pertumbuhan tertinggi padapeubah tinggi bibit, bobot basah, dan bobotkering diperoleh pada inokulasi sebanyak 500spora per polibag baik untuk A. tuberculatamaupun G. margarita. Namun, pada inokulasisebanyak 500 spora per polibag, pertumbuhandan serapan N, P, dan K bibit yang diinokulasiA. tuberculata dan G. margarita tidak berbedanyata kecuali pada peubah bobot basah akar danbobot basah bibit. Bobot basah akar dan bobotbasah bibit kelapa sawit yang diinokulasiA. tuberculata sebanyak 500 spora, lebih tinggidibandingkan dengan bibit yang diinokulasidengan G. margarita pada jumlah spora yangsama. Pengaruh spesies hanya dapat ditunjukkanpada inokulasi 200 dan 350 spora khususnya padapeubah tinggi bibit, bobot basah, dan bobotkering bibit. Tinggi bibit, bobot basah dan bobotkering bibit yang diinokulasi A. tuberculata padajumlah spora 200 dan 350 per polibag lebih tinggidibandingkan dengan yang diinokulasiG. margarita. Tampak bahwa inokulasiA. tuberculata dengan 200 spora per polibagmenghasilkan serapan N dan K lebih tinggidibandingkan dengan yang diinokulasiG. margarita pada jumlah spora yang sama.


2016 ◽  
Vol 73 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
Nampiah SUKARNO ◽  
Latifah Kosim DARUSMAN ◽  
Didiek Hadjar GOENADI ◽  
Sally SMITH ◽  
...  

SummaryA green house experiment was conducted tostudy the effect of spore number and species ofAM fungi as inoculant of oil palm. Two species ofAM fungi was evaluated in this study namelyAcaulospora tuberculata and Gigaspora margaritaand three spore number were tested i. e 200, 350,and 500 spores. There two fungi have thepotential as AM fungi inoculant for oil palm. Thesoil used was acid soil from Cikopomayak, WestJava while the oil palm seedling was from OilPalm Research Institute, Medan. A polybag sized20 x 40 cm was used. Spores as type of inoculantaffect the oil palm growth in longer time. Thebest growth of the seedling in term of height,fresh, and dry weight was obtained byinoculation at 500 spores of A. tuberculata andG. margarita. However, at 500 spores perpolybag, growth and N, P, and K uptake ofseedlings inoculated with A. tuberculata andG. margarita were not significantly differentexcept for seedling and root fresh weight. Oilpalm seedling inoculated with A. tuberculata at500 spores per seedling resulted higher root andseedling fresh weight compared with thoseinoculated with G. margarita. The different effectof seedling on A. tuberculata and G. margaritainoculation at 200 and 350 spores per seedlingwere only observed in plant height, fresh and dryweight of seedlings. The plant height, fresh, anddry weight of seedlings inoculated withA. tuberculata at 200 and 350 spores per seedlingwere higher compared with those inoculatedwith G. margarita. In addition inoculation withA. tuberculata at 200 spores per seedling resultedhigher N and K uptake of seedling compared withthose inoculated with G. margarita.RingkasanSuatu penelitian rumah kaca telah dilakukanuntuk mempelajari pengaruh jumlah spora danspesies cendawan mikoriza arbuskula (CMA)sebagai inokulum pada bibit kelapa sawit. Duaspesies CMA yang diuji ialah Acaulosporatuberculata dan Gigaspora margarita sedangkanjumlah spora yang diuji ada tiga tingkat yaitu200, 350, dan 500 spora. Bibit kelapa sawitberumur dua bulan ditanam di polibag berukuran20 x 40 cm yang berisi tanah yang bereaksimasam berasal dari Cikopomayak. Hasil yangdiperoleh menunjukkan bahwa spora sebaganokulum bibit kelapa sawit dapat mempengaruhipertumbuhan kelapa sawit namun diperlukanwaktu yang lebih lama untuk mendapatkanrespons inokulasi. Pertumbuhan tertinggi padapeubah tinggi bibit, bobot basah, dan bobotkering diperoleh pada inokulasi sebanyak 500spora per polibag baik untuk A. tuberculatamaupun G. margarita. Namun, pada inokulasisebanyak 500 spora per polibag, pertumbuhandan serapan N, P, dan K bibit yang diinokulasiA. tuberculata dan G. margarita tidak berbedanyata kecuali pada peubah bobot basah akar danbobot basah bibit. Bobot basah akar dan bobotbasah bibit kelapa sawit yang diinokulasiA. tuberculata sebanyak 500 spora, lebih tinggidibandingkan dengan bibit yang diinokulasidengan G. margarita pada jumlah spora yangsama. Pengaruh spesies hanya dapat ditunjukkanpada inokulasi 200 dan 350 spora khususnya padapeubah tinggi bibit, bobot basah, dan bobotkering bibit. Tinggi bibit, bobot basah dan bobotkering bibit yang diinokulasi A. tuberculata padajumlah spora 200 dan 350 per polibag lebih tinggidibandingkan dengan yang diinokulasiG. margarita. Tampak bahwa inokulasiA. tuberculata dengan 200 spora per polibagmenghasilkan serapan N dan K lebih tinggidibandingkan dengan yang diinokulasiG. margarita pada jumlah spora yang sama.


Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 130
Author(s):  
Flor Hernandez ◽  
Rosalinda Villarreal ◽  
Valentin Torres ◽  
Adrien Gallou

Research into the symbiotic relationship between plants and arbuscular mycorrhizal fungi (AMF) is key for sustainable agricultural intensification. The objective of the present study is to evaluate native AMF at the monosporic level in greenhouse-grown, economically important crops. Agricultural soil samples from three locations (Saltillo, Zaragoza, and Parras) were obtained by combining portions resulting from a zigzag sampling pattern. From these samples, 15 morphotypes were extracted according to a modified Gerdemann’s technique and monosporically inoculated on melon, cucumber, tomato, and onion, 30 days after their sowing. Under a completely random experimental design, 16 treatments with three repetitions were defined. Plant height, root length, stem diameter, total fresh weight, fresh root weight, dry root weight, bulb weight, fresh leaf weight, total dry weight, flower number, leaf number, fruit number, spore number, and percentage of colonization were all evaluated. The results were subjected to the analysis of variance (ANOVA) and the Tukey comparison test (p ≤ 0.05), which showed that the monosporic inoculation favors significantly the AMF and the host, while the T6 (Saltillo spore + Steiner modified with 20% of the normal phosphorus concentration) showed a greater response uniformity on onion and melon, which indicates its great potential as an inoculum.


2007 ◽  
Vol 64 (4) ◽  
pp. 393-399 ◽  
Author(s):  
Milene Moreira ◽  
Dilmar Baretta ◽  
Siu Mui Tsai ◽  
Sandra Maria Gomes-da-Costa ◽  
Elke Jurandy Bran Nogueira Cardoso

Araucaria angustifolia (Bert.) O. Ktze. is an endangered Brazilian coniferous tree that has been almost exterminated in the native areas because of uncontrolled wood exploitation. This tree has been shown to be highly dependent on arbuscular mycorrhizal fungi (AMF) and, therefore, AMF may be essential for forest sustainability and biological diversity. Root colonization, density and diversity of AMF spores were assessed in two Araucaria forest stands at the State Park of Alto Ribeira (PETAR), at two sampling dates: May and October. A comparison was made between a mature native stand composed of Araucaria trees mixed into a variety of tropical trees and shrubs, without any sign of anthropogenic interference (FN) and an Araucaria stand planted in 1987 (R), which has been used as a pasture. Assessments included percent root colonization, AMF spore numbers and species richness, Simpson's dominance index (Is), and Shannon's diversity index (H). Mycorrhizal root colonization did not differ between ecosystems in May. In October, however, the native stand (FN) presented a higher colonization than the planted forest (R), and the root colonization was more intense than in May. When considering both sampling periods and forests, 27 species of AM fungi, with higher numbers of spores in FN than in R were found. Canonical discriminant analysis (CDA) indicated Shannon's diversity index as the ecological attribute that contributed the most to distinguish between forest ecosystems, with higher value of H in FN in relation to R. CDA showed to be a useful tool for the study of ecological attributes.


2002 ◽  
Vol 82 (3) ◽  
pp. 272-278 ◽  
Author(s):  
A. Liu ◽  
C. Hamel ◽  
A. Elmi ◽  
C. Costa ◽  
B. Ma ◽  
...  

Little attention has been paid to the effect of arbuscular mycorrhizal (AM) fungi on the uptake of nutrients that move mainly by mass flow. The objective of this study was to assess the possible contribution of indigenous AM fungi to the K, Ca and Mg nutrition of maize (Zea mays L.) as influenced by soil P levels and its impact on plant dry mass. The field experiment had a split plot design with four replicates. Treatments included soil fumigation status (fumigation and non-fumigation) and three levels of P fertilization (0, 60 and 120 kg P2O5 ha-1) in a loamy sand soil in 1997 and a fine sandy loam soil in 1998. Soil fumigati on with Basamid® was used to suppress indigenous AM fungi. Plants were sampled at four different growth stages (6-leaf stage, 10-leaf stage, tasseling and silking). Soil fumigation decreased shoot dry weight, but P fertilization increased shoot dry weight at most sampling times. When no P fertilizer was added, fumigation in the loamy sand soil reduced shoot K and Ca concentrations while, in contrast, in the fine sandy loam soil only Mg concentration was reduced by soil fumigation. The concentration of K in maize shoots was positively correlated (P < 0.05) with extraradicular hyphal length in both soils. The correlation between the abundance of extraradicular hyphae and the concentrations of Ca and Mg in maize shoots was significant only for soils where available Ca or Mg was relatively low. Arbuscular mycorrhizal fungi could increase corn biomass production and K, Ca and Mg uptake in soil low in these elements and low in P. These results indicate that the contribution of mycorrhizae to maize K, Ca and Mg nutrition can be significant in a field situation and that the extent of this contribution depends on the availability of these nutrients and of P in soils. Key words: Arbuscular mycorrhizal fungi, soil fumigation, extraradicular hyphae, uptake of K, Ca, and Mg, soil P levels, maize


2012 ◽  
Vol 63 (2) ◽  
pp. 164 ◽  
Author(s):  
B. A. L. Wilson ◽  
G. J. Ash ◽  
J. D. I. Harper

Messina [Melilotus siculus (Turra) Vitman ex. B. D Jacks] is a salt- and waterlogging-tolerant annual legume that could be highly productive on saline land. Arbuscular mycorrhizal (AM) fungi form a symbiotic relationship with the majority of terrestrial plant species, and improved productivity of plants inoculated with AM fungi under saline conditions has been attributed to the increased uptake of nutrients such as phosphorus (P). However, the mycorrhizal status of M. siculus under saline or non-saline conditions is unknown, as is the role of AM in improved nutrition and nodulation. In this study, the role of AM fungi in growth improvement and nodulation of M. siculus was examined in saline and non-saline soil. The M. siculus plants were inoculated with either a single AM species or mixed AM species, or remained uninoculated, and were grown at three levels of sodium chloride (NaCl) (0, 80, and 250 mm NaCl). AM-inoculated plants had significantly greater nodulation than plants that did not receive AM inoculum, regardless of salinity level. Plants inoculated with mixed AM species at 250 mm NaCl showed improved survival (90%) compared with the plants inoculated with single AM species or uninoculated control plants (30%). Within each salinity level, plants inoculated with mixed AM species had significantly greater dry weight than all other treatments. In addition, plants inoculated with mixed AM species had increased total uptake of P. It is likely that the increased growth observed in AM-inoculated M. siculus plants is due to improved P nutrition, showing the potential of AM fungi to enhance the growth of M. siculus on saline land.


2014 ◽  
Vol 16 (2) ◽  
pp. 44
Author(s):  
Dhimas Taufika Putra ◽  
Samanhudi Samanhudi ◽  
Purwanto Purwanto

<p>Ginger (<em>Zingiber officinale</em>) is one kind of plants to be processed as traditional medicine, drinks, and spices. the smallest type of ginger that has many benefits as medical plant. Demand for ginger plants growing in the community is increasing. Additions of manure and arbuscular mychorrhizal fungi (AMF) can increase the quality and quantity of ginger. This was conducted to obtain the kinds of manure and levels of AMF to ginger to produce optimum growth and yield. This research used a completely randomized design (CRD) consisting of two treatment factors, namely planting medium and arbuscular mycorrhizal fungi (AMF). Results were analyzed by analysis of variance and if there is a real difference continued DMRT 5% level. The results showed that the differences in the kinds of manure and the levels of AMF increase growth and yield of ginger. Chicken manure application showed the highest results in the fresh weight of stover plant, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes. Whereas the additions of AMF 15 grams per plant in zeolite showed the highest results in the number of plant tillers, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes.</p>


2020 ◽  
Vol 4 (1) ◽  
pp. 36-43
Author(s):  
Jaya Thakur ◽  
Bharat Shinde

The study was conducted to determine the effect of arbuscular mycorrhizal (AM) fungi inoculation on growth of pea grown under water stressed pot culture conditions. Water stress was given to the pea plants after 30 days at the interval of 4, 8 and 12 days. The data was collected at an interval of 15 days. Three replicates of each set were maintained. . The mixture of AM fungi used for current experiment included the species of Acaulospora denticulata, A. gerdemannii, Glomus macrocarpum, G. maculosum, G. fasciculatum and Scutellospora minuta. The mycorrhizal plants have shown more shoot and root length as compared to the control plants. The height of shoot and root was significantly decreased with the increase in drought stress. Mycorrhizal plants with low water stress showed enhanced shoot and root length than high water stress. The mycorrhizal plants have shown more number of leaves than control plants during drought stress. The number of leaves significantly reduced with the increase in drought stress. The leaves produced by the control plants were comparatively smaller than those of mycorrhizal plants. The dry weight of root and shoot of both control and mycorrhizal plants decreased with the increase in water stress. Mycorrhizal plants showed more dry weight of shoot and root as compared to control plants.  Plants inoculated with AM fungi produce more dry weight than the control plants. The fresh weight of both control and mycorrhizal plants has been decreased with the increase in water stress interval and also the fresh weight of root and shoot was observed higher in mycorrhizal plants as compared to those of control plants.


2021 ◽  
Vol 914 (1) ◽  
pp. 012049
Author(s):  
D Prameswari ◽  
R S B Irianto ◽  
F D Tuheteru ◽  
T Kalima

Abstract A screw tree (Helicteres isora L.) is a small/large shrub species that grows and spreads in many Asian countries, including Indonesia (NTT and Maluku). It is a medicinal plant commonly used to treat many diseases, such as bleeding and constipation. This study aimed to determine the effect of Arbuscular Mycorrhizal (AM) fungi and planting media on the growth of screw tree seedlings in a nursery. This research was conducted at Bogor Forest Research and Development Center’s nursery, Indonesia. This study consisted of two factors: AM fungi with three levels, namely control, Glomus aggregatum and Glomus clarum and growth media with two levels, namely mixed media of soil: rice husk charcoal (2:1) and mixed soil of media: rice husk charcoal: cocopeat (2:1:1). The results showed that treatment of G. aggregatum and soil mixed of media: rice husk charcoal: cocopeat (2:1:1) was significantly different from other treatments except for G. clarum and soil mixture of media: rice husk charcoal: cocopeat (2:1:1) that significantly increased height, diameter and dry weight of seedlings and the values were 97, 56, 126 and 46, 37, 127% compared to the control. Mycorrhizal dependency of screw tree was very high (126 and 127%). Generally, interaction treatment of G. clarum and mixed media of soil: rice husk charcoal: cocopeat (2:1:1) increased the growth of 11-month-old screw tree in the nursery.


2018 ◽  
Vol 47 (1) ◽  
pp. 221-226 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Livio TORTA ◽  
Giorgio FERRARA ◽  
Giovanni IAPICHINO

Herbaceous plants used in island beds and borders need to be rapid growing, high performing and maintaining good visual quality during the growing season. Arbuscular mycorrhizal (AM) fungi application is acquiring interest for its beneficial effects on ornamental bedding plants. Gazania rigens is a herbaceous ornamental plant grown for its large daisy-like flowers. The species thrives in the coastal areas of the Mediterranean region, particularly in the mild climate of southern Italy and Sicily, where performs well in summer bedding schemes in sea side gardens even in dry and windy conditions. The aim of this study was to evaluate the effect of inoculation with Rhizophagus irregularis on several ornamental parameters of Gazania rigens. Prior to transplanting, three-months-old plants received a mycorrhizal inoculum carrying 40 spores g-1 of Rhizophagus irregularis. Inoculum was applied at a rate of 10 g plant-1. The AM application significantly increased number of flowers per clump by 100% and number of flowers per plant by 124.0%. Rhizophagus irregularis also positively influenced number of leaves per plant, plant height, and roots dry weight. Our findings indicated that mycorrhizal inoculation with R. irregularis may be beneficial to nursery growers wishing to produce high quality gazania for spring-summer bedding plant schemes.


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