scholarly journals CAN Stryphnodendron adstringens EXTRACT IMPROVE THE RESULTS OF FISTULOTOMY FOLLOWED BY PRIMARY SPHINCTEROPLASTY IN THE TREATMENT OF TRANSSPHINCTERIC FISTULAE?

2020 ◽  
Vol 33 (3) ◽  
Author(s):  
Adriana de Souza RÓS ◽  
Carlos Henrique Marques dos SANTOS ◽  
Doroty Mesquita DOURADO ◽  
Moisés Soares da SILVA-NETO ◽  
Isabela CALDEIRA ◽  
...  
Keyword(s):  
Anal Canal ◽  
Wistar Rats ◽  
Inflammatory Process ◽  
Histological Analysis ◽  
Steel Wire ◽  
Fistulous Tract ◽  
Herbal Medicines ◽  
Group A ◽  
Group B

ABSTRACT Background: There is still a need for progress in the treatment of transsphincteric anal fistulae and the use of herbal medicines seems promising. Aim: To evaluate the efficacy of Stryphnodendron adstringens associated with fistulotomy and primary sphincteroplasty in the treatment of transsphincteric fistulae in rats. Methods: Thirty Wistar rats were used, which were submitted to transsphincteric fistulas with steel wire 0; after 30 days a treatment was performed according to the group. Group A (n=10) was submitted to fistulotomy; group B (n=10), fistulotomy followed by primary sphincteroplasty with “U” stitch with polyglactin 911 4-0; group C(n=10) , similar to group B, but with the interposition between the muscular stumps of hemostatic sponge soaked in Stryphnodendron adstringens extract. Euthanasia was performed after 14 days, resecting a segment of the anal canal for histological analysis, which aimed to evaluate the closure of the fistula, the area of separation of the muscle cables, the inflammatory process and the degree of fibrosis. Results: No animal had a remaining fistulous tract. About the spacing between the muscle cables, an average of 106.3 µm2 was observed in group A, 82.8 µm2 in group B and 51.8 µm2 in group C (p<0.05). There was no difference between the groups regarding the inflammatory process and, in relation to fibrosis, in group A there was a mean of 0.6, in group B 0.7 and in group C 0.2 (p<0.05). Conclusions: Stryphnodendron adstringens extract was able to allow less spacing between muscle cables in rats submitted to fistulotomy followed by primary sphincteroplasty, in addition to providing less local fibrosis.

scholarly journals Comparative study between polypropylene and polypropylene/poliglecaprone meshes used in the correction of abdominal wall defect in rats

2012 ◽  
Vol 27 (4) ◽  
pp. 300-305 ◽  
Author(s):  
Carlos Alberto Lima Utrabo ◽  
Nicolau Gregori Czeczko ◽  
Cesar Roberto Busato ◽  
Mario Rodrigues Montemor-Netto ◽  
Osvaldo Malafaia ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Wistar Rats ◽  
Inflammatory Process ◽  
Abdominal Wall Defect ◽  
Healing Process ◽  
Break Point ◽  
Wall Defect ◽  
Chronic Inflammatory Process ◽  
Group A ◽  
Group B

PURPOSE: To evaluate the healing process of a defect in the ventral abdominal wall of rats, comparing the polypropylene and polypropylene/poliglecaprone meshes on the 30th and 60th postoperative day. METHODS: Thirty two Wistar rats were submitted to a ventral abdominal wall defect, with integrity of the parietal peritoneum. In the repair, were used polypropylene (group A) and polypropylene/poliglecaprone (group B) meshes. The groups were subdivided into four subgroups of eight animals euthanized on the 30th (A30 and B30) and 60th postoperative day (A60 and B60). Fragments of the abdominal wall of the animals were submitted to macroscopic, tensiometric and histological evaluations. RESULTS: The tensiometry on subgroup A30 showed a mean average break point of 0.78 MPa and in A60, 0.66 Mpa. In subgroup B30 it was 0.84 MPa and in B60, 1.27 Mpa. The score of the inflammatory process showed subacute phase on A30 and B30 sub-groups and chronic inflammatory process in subgroups A30 and 60B. CONCLUSIONS: The tensile strength was higher on the wall repaired by polypropylene/poliglecaprone mesh in the 60th post-operative day. Histology showed higher concentration of fibrosis on the surface of the polypropylene mesh with a tendency to encapsulation. In polypropylene/poliglecaprone subgroups the histology showed higher concentration of fibrosis on the surface of mesh filaments.

scholarly journals PRIMARY SPHINCTEROPLASTY COMPARING TWO DIFFERENT STITCHES IN ANAL FISTULA TREATMENT: EXPERIMENTAL STUDY IN RATS

2019 ◽  
Vol 32 (3) ◽  
Author(s):  
Otávio Augusto Vendas TANUS ◽  
Carlos Henrique Marques dos SANTOS ◽  
Doroty Mesquita DOURADO ◽  
Andrea Lima CONDE ◽  
Fernanda GIUNCANSE ◽  
...  
Keyword(s):  
Experimental Study ◽  
Anal Canal ◽  
Anal Fistula ◽  
Wistar Rats ◽  
Steel Wire ◽  
Histopathological Analysis ◽  
Analysis Measurement ◽  
Group A ◽  
Perineal Region ◽  
Fistula Treatment

ABSTRACT Background: Anal fistula is by definition the communication between the rectum or anal canal with the perineal region, which may be epithelialized and has cryptoglandular origin in 90% of cases. There are a large number of techniques for successfully treating trans-sphincteric fistulas of 20-50%, including primary sphincteroplasty, but it is not clear whether the material used would influence the outcome. Aim: To analyze the efficacy of polydioxanone and polypropylene wire in primary post-fistulotomy sphincteroplasty in the treatment of trans-sphincteric fistulas in rats. Methods: Thirty Wistar rats had transfixation of the anal sphincter with steel wire, which remained for 30 days for the development of the anal fistula. After this period, it was removed and four groups were formed: A (control) without treatment; B (fistulotomy) submitted to such procedure and curettage only; C (polidioxanone) in which sphincteroplasty was performed after fistulotomy with polydioxanone wire; D (polypropylene) submitted to the same treatment as group C, but with polypropylene wire. After 30 days, euthanasia and removal of the specimens were performed for qualitative histopathological analysis, measurement of the area between the muscular cables edges and evaluation of the degree of local fibrosis. Results: There was persistence of the anal fistula in all animals of group A. There were no significant differences between groups B, C and D regarding the distance of the muscular cables (p=0.078) and the degree of fibrosis caused by the different treatments (p=0.373). Conclusions: There was no difference between polydioxanone and polypropylene wires in post-fistulotomy primary sphincteroplasty, and this technique was not superior to simple fistulotomy in relation to the distance of the muscular cables nor did it present differences in relation to the degree of local fibrosis.

scholarly journals The effects of Roux-en-Y limb length on gastric emptying and enterogastric reflux in rats

2008 ◽  
Vol 23 (2) ◽  
pp. 179-183 ◽  
Author(s):  
Robson Azevedo Dutra ◽  
Wemberton Martins Araújo ◽  
José Ivan de Andrade
Keyword(s):  
Gastric Emptying ◽  
Wistar Rats ◽  
Limb Length ◽  
Roux Limb ◽  
Short Limb ◽  
Male Wistar Rats ◽  
Group A ◽  
Group B ◽  
Enterogastric Reflux

PURPOSE: To assess the effects of Roux-en-Y jejunal limb length on gastric emptying and enterogastric reflux. METHODS: Seventy male Wistar rats were submitted to antrectomy with Roux-en-Y reconstruction and then were divided into two groups of 35 animals. Group A, short limb (7.5 cm) and Group B, standard limb (15 cm). Group A and B were subdivided into five subgroups each in order to study enterogastric reflux at 30 and 60 minutes and to evaluate gastric emptying at 5, 10 and 15 minutes. In order to measure gastric emptying and enterogastric reflux, radiotracers 99m Tc-Phytate and 99m Tc-DISIDA were respectively used. RESULTS: For gastric emptying, the radiotracer concentration was lower in Group A than in Group B after five minutes. The enterogastric reflux was present, but there were no significant differences between enterogastric reflux indexes concerning both A and B Groups. CONCLUSION: A standard Roux limb, besides being unable to protect the stomach from the enterogastric reflux, may become a functional barrier for gastric emptying.

scholarly journals RADIOLOGICAL STUDY OF MEGACOLON IN TRYPANOSOMA CRUZI INFECTED RATS

2018 ◽  
Vol 31 (1) ◽  
Author(s):  
Carlos Edmundo Rodrigues FONTES ◽  
Ana Paula de ABREU ◽  
Aretuza Zaupa GASPARIM
Keyword(s):  
Trypanosoma Cruzi ◽  
Wistar Rats ◽  
In Vivo Studies ◽  
Proposed Model ◽  
Male Wistar Rats ◽  
Group A ◽  
Time Of Infection ◽  
Group B ◽  
Digital Caliper

ABSTRACT Background: Researches on Chagas disease still use several animals and rats, due to size and susceptibility were preferred by many authors. Aim: To develop an experimental model of megacolon in rats inoculated with the strain Y of Trypanosoma cruzi. Methods: Thirty male Wistar rats were distributed in three groups inoculated with different inoculants: Group A: 600000, Group B: 1000000 and Group C: 1500000 blood trypomastigotes of T. cruzi. Animals were sedated intramuscularly at zero inoculation time (T0) and 60 days after inoculation (T60), to perform the barium enema in order to evaluate the dilatation of the different segments of colon in a comparative study of the measurements obtained, using a digital caliper. Evidence of infection was performed by blood smear collected from the animal’s tail 18 days after inoculation with observation of blood forms. Results: Comparing the intestinal diameter of the inoculated animals with 60,0000 trypomastigotes in the T0 of infection with T60 days after the inoculation, significant dilatation was observed between the proximal, medial and distal segments (p<0.01), indicating the establishment of the megacolon model. In addition, comparing intestinal diameter between the different segments, with in the T0 of infection and the T60 after inoculation, significant alterations were observed (p<0.05). Conclusion: The proposed model was possible for in vivo studies of alterations due to infection by T. cruzi and functional alterations of the colon. In addition, the changes manifested in the colon are not directly proportional to the size of the inoculum, but to the time of infection that the animals were submitted, since the animals inoculated with 60,0000 blood forms were the ones which presented the most significant alterations.

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Histological studies on the retina and cerebellum of Wistar rats treated with Arteether™

2014 ◽  
Vol 31 (01) ◽  
pp. 028-032
Author(s):  
A. Okunlola ◽  
C. Okunlola ◽  
C. Okani ◽  
O. Adewole ◽  
D. Ofusori ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Study Group ◽  
Group A ◽  
Cervical Dislocation ◽  
Nissl Stain ◽  
Group B ◽  
Haematoxylin And Eosin Stain ◽  
Therapeutic Doses ◽  
Cellular Components ◽  
Group D

Abstract Introduction: Arteether™, a derivative of artemisinin, is among the recent drugs that have given renewed hope for combating malarial menace. The present study investigated the effects of arteether™ on the histology of the retina and cerebellum of Wistar rats. Materials and Methods: Twenty adult albino Wistar rats weighing 150-200 g, were randomly divided into four groups (A, B, C and D) of five animals each and used for this study. Group A rats were given intramuscular (i.m.) arteether™ (3 mg/kg b.w.) daily for 3 days. Group B rats were given i.m. arteether™ (6 mg/kg b.w.) daily for 3 days. Group C rats were also given i. m. of arteether™ (3 mg/kg b. w.) daily for 3 days, and the same dose was repeated at two-weekly intervals for 4 further weeks; while Group D rats which received normal saline (0.9 % w/v, 3 ml/kg b.w.), served as controls. At the end of the experiment, the rats were sacrificed by cervical dislocation. The retina and cerebellum were excised and processed routinely for histopathology changes, using haematoxylin and eosin stain (H & E), as well as Nissl stain. Results: Results obtained showed normal cellular components of the retina and cerebellum in all groups, and no cyto-pathological changes were observed. Conclusion: Thus, this study showed that under light microscopic examination, therapeutic doses of arteether™ caused no significant cyto-pathologic changes in the retina and cerebellum of Wistar rats.

Effectiveness of Cuscuta planiflora Ten. and Nepeta menthoides Boiss. & Buhse in Major Depression

2014 ◽  
Vol 20 (2) ◽  
pp. 94-97 ◽  
Author(s):  
Ali Firoozabadi ◽  
Mohammad M. Zarshenas ◽  
Alireza Salehi ◽  
Saye Jahanbin ◽  
Abdolali Mohagheghzadeh
Keyword(s):  
Major Depression ◽  
Common Mental Disorders ◽  
Herbal Medicines ◽  
Cost Benefit ◽  
Controlled Clinical Trial ◽  
Medical Sciences ◽  
Before And After ◽  
Group A ◽  
Traditional Persian Medicine ◽  
Group B

Background. Depression is one the most common mental disorders that can be seen all over the world. In traditional Persian medicine, some medicinal herbs are recommended for depression treatment. This study aimed to evaluate the effects of Cuscuta planiflora Ten. and Nepeta menthoides Boiss. & Buhse in patients with major depression. Methodology. This study is a randomized triple-blind controlled clinical trial conducted in the year 2010 in Shiraz University of Medical Sciences on patients with major depression. Pharmaceutical capsules of Cuscuta planiflora (500 mg) and Nepeta menthoides (400 mg) were prepared by a pharmacist. Patients were randomly assigned to 3 groups: group A (treated with Nepeta menthoides capsules and conventional drugs), group B (treated with Cuscuta planiflora capsules and conventional drugs), and group C (treated only with conventional drugs). The study period was 8 weeks and depression was measured before and after the study by Beck Depression Inventory and Hamilton Depression Inventory. The data were analyzed by SPSS version 20 and the P < .05 was considered statistically significant. Results. A total of 43 subjects participated in this study, of whom 81.4% were females (n = 35) and 18.6% were males (n = 8). The mean ± standard deviation of age of the participants was 38 ± 10.9 years. The majority of patients (65.1%, n = 28) were married. There were 15 patients (34.9%) in group A, 13 (30.29%) in group B, and 15 (34.9%) in group C. There was a significant decrease in mean scores of Beck and Hamilton depression inventories in the 3 groups after treatment ( P < .01); moreover, there was more decrease in scores of the Beck and Hamilton depression inventories in groups A and B compared with group C after treatment ( P < .01). Conclusion. Despite the paucity of the population under study, the findings showed that Cuscuta planiflora and Nepeta menthoides capsules could be effective, affordable herbal medicines with improved cost–benefit in treatment of major depression and it is worth designing further and more extensive studies to get to a more accurate conclusion.

scholarly journals Therapeutics potential of Ocimum basilicum following mercury chloride-induced hepatotoxicity in rats (Rattus norvegicus)

2018 ◽  
Vol 5 (11) ◽  
pp. 725-737
Author(s):  
Sunday A. Adelakun ◽  
Olusegun D. Omotoso ◽  
Grace T. Akingbade
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Ocimum Basilicum ◽  
Alanine Transaminase ◽  
Mercury Chloride ◽  
Antioxidant Parameters ◽  
Group A ◽  
Group B ◽  
Anatomical Parameters

Globally mercury (Hg) has been reported as one of heavy metal of known toxicity, noted for inducing public health disasters. Present study examines the therapeutics potentials of Ocimum basilicum on mercury chloride (HgCl2) induced hepatotoxicity in Wistar rats. Thirty adult Wistar rats randomly divided into six groups (A-F) of five rats each. Group A served as control was given 2 mL/day of distilled water, Group B, C, D, E and F received 500 mg/kg body weight (bwt) of O. basilicum extract, 20 mg/kg/bwt of HgCl2, 40 mg/kg bwt of HgCl2, 20 mg/kg bwt of HgCl2 and 500 mg/kg bwt O. basilicum leave extract, 40 mg/kg bwt and 500 mg/kg bwt O. basilicum administered daily by gastric gavage, for 21 consecutive days. The gross anatomical parameters of the liver and liver histology were assessed. Liver oxidative stress was evaluated by liver superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and malondialdehyde (MDA) assays. The activities of the biomarker enzymes of the liver (alanine transaminase, aspartate transaminase and alkaline phosphatase were assayed). Histological profiles of the liver revealed derangement of the liver cytoarchitecture following consumption of mercury chloride and a marked improvement was observed after O. basilicum administration. Similarly, O. basilicum improved the reduction of antioxidant parameters (SOD, CAT, GPx and GSH) and the increased MDA caused by mercury chloride consumption. O. basilicum thus proffer protection against free radical mediated oxidative stress in mercury chloride-induced hepatotoxicity in rats.

scholarly journals Effects of Crude Extract of Neem Bark on the Pancreas of Streptozotocin Induced Diabetic Wistar Rats

2019 ◽  
pp. 1-9
Author(s):  
A. J. Ajibade ◽  
E. O. James ◽  
B. D. Kehinde
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Crude Extract ◽  
Wistar Rats ◽  
Enzymatic Activities ◽  
Diabetic Group ◽  
Bark Extract ◽  
Group A ◽  
Group B

This study investigated the histological and serum enzymatic activities of Azadirachta indica, an Indian medicinal plant, on the pancreas in streptozotocin induced diabetic adult wstar rats. Forty six adult wistar rats weighing 100 g to 220 g were randomly separated into four groups; Group A was regarded as the control , while group B was the diabetic group, C and diabetic – extract treated group. The control group received distilled water throughout the experiment; the remaining three groups were induced with streptozotocin intra-peritoneally to induced diabetes in the wistar rats. After some days, the animals were confirmed diabetic with the help of a measuring glucometer. Thereafter, group B diabetic rats remained untreated while Group C and D were treated with a low dose (250 mg/kg) of the crude neem bark extract and a high dose (500 mg/kg) of the extract respectively for 42 days. The aqueous neem bark extract was suspended in the drinking water of the treated animals for the period of 42 days. The body weights of the animals were weighed weekly and likewise the measurement of the blood glucose level was taken. The animals were sacrificed at the end of 42 days by cervical dislocation and the pancreas was removed and weighed immediately using sensitive weighing balance. The blood samples were collected from the sacrificed animals into EDTA bottle for serum enzymatic analysis. The organ pancreas was fixed in a 10% formol saline, processed and stained with Heamatoxylin and Eosin for general histological study. The microscopic examination of diabetic group showed some degenerative and necrotic cells which made the pancreatic tissue distorted compared with the control that appeared normal. The diabetic group C and D rats treated with the extract showed ameliorative potentials of the extract with improvement in histo-architecture of the pancreatic tissue following recovery from damage. Analysis of the blood serum level showed that the aqueous neem bark extract has ameliorative effect on the enzymatic activities in serum of the treated rats. The alanine amino transferase, aspartate amino transferase and alkaline phosphatase levels were significantly reduced (P<0.05) in the crude extract- treated animals compared with the untreated group B and control group A with significantly increased ( P<0.05 ) enzymatic activities in wistar rats in these groups. The antioxidant status was compromised in diabetic group B with significantly increased (P<0.05) LPO, reduced SOD and GSH compared with significantly reduced (P<0.05) LPO, increased (P<0.05) SOD and GSH in group A- control and Group C and D diabetic-extract treated Wistar rats. Similarly, the blood glucose level increased significantly (P<0.05) in group B diabetic group compared with significantly reduced (P<0.05) blood glucose level in group A control as well as C and D extract-treated rats. The study concluded that crude extract of neem has ameliorative potentials on streptozotocin-induced diabetic wistar rats characterized by oxidative damage which reveals improvement in tissue morphology.

scholarly journals Pengaruh pemberian ekstrak daun cengkeh (Syzygium aromaticum) terhadap gambaran histopatologik hati tikus wistar yang diberikan parasetamol dosis toksik

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Denty M. Sidabutar ◽  
Carla F. Kairupan ◽  
Meilany Durry
Keyword(s):  
Single Dose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Cell Damage ◽  
Liver Cells ◽  
Syzygium Aromaticum ◽  
Toxic Dose ◽  
Group A ◽  
Group B ◽  
Group D

Abstract: Clove (Syzygium aromaticum) contains eugenol, a phenolic compound, which has been suggested to possess antioxidant activity. This compound is suspected to be able to minimize damage to the liver cells caused by drugs such as paracetamol. This study aimed to observe the effects of clove leaf extract on histopathological features of Wistar rat liver tissue induced with toxic doses of paracetamol. This was an experimental laboratory study. Subjects were 24 male Wistar rats. The dose of clove leaf extract was 200 mg/day (single dose) and of paracetamol 50 mg/day (single dose) orally. Group A (negative control) was given no treatment for 14 days. Group B was given paracetamol for 14 days. Group C was given clove leaf extract for 7 days and then added with paracetamol for 7 days. Group D was given clove leaf extract and paracetamol simultaneously for 14 days. Group A showed normal histological feature of liver cells. Group B showed liver cell damage induced by paracetamol. Group C showed regeneration of liver cells, but there were still some necrosis and fatty liver cells. Group D showed regeneration of liver cells meanwhile cell necrosis was hardly found. Conclusion: Clove leaf extract could improve the histopathological changes of liver tissues of Wistar rats due to administration of paracetamol at toxic dose. This improvement was manifested as better regeneration of liver cells than that of rats not treated with clover leaf extract. Keywords: clove, paracetamol, liver Abstrak: Cengkeh (Syzygium aromaticum) mengandung senyawa eugenol, suatu komponen fenolik yang memiliki aktivitas antioksidan. Senyawa eugenol diduga dapat meminimalisir kerusakan sel hati yang antara lain disebabkan oleh obat-obatan yang berefek hepatototoksik seperti parasetamol. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun cengkeh terhadap gambaran histopatologik hati tikus wistar yang diinduksi dengan parasetamol dosis toksik. Jenis penelitian ini eksperimental laboratorik. Subjek penelitian 24 ekor tikus wistar. Pada penelitian ini digunakan ekstrak daun cengkeh 200 mg/hari (dosis tunggal) dan obat parasetamol 50 mg/hari (dosis tunggal) per oral. Subjek penelitian dibagi menjadi 4 kelompok. Kelompok A (kontrol negatif) tidak diberi perlakuan selama 14 hari. Kelompok B diberikan parasetamol selama 14 hari. Kelompok C diberikan ekstrak daun cengkeh selama 7 hari kemudian ditambahkan parasetamol secara bersamaan selama 7 hari. Kelompok D diberikan ekstrak daun cengkeh dan parasetamol secara bersamaan selama 14 hari. Kelompok A memperlihatkan gambaran histopatologik sel hati normal. Kelompok B memperlihatkan kerusakan sel hati berupa nekrosis dan perlemakan sel. Kelompok C memperlihatkan regenerasi sel hati namun masih terdapat nekrosis dan perlemakan sel hati. Kelompok D memperlihatkan regenerasi sel hati yang luas dan hampir tidak ditemukan nekrosis sel. Simpulan: Pemberian ekstrak daun cengkeh memperlihatkan perbaikan gambaran histopatologik jaringan hati tikus wistar yang mengalami kerusakan akibat parasetamol dosis toksik berupa regenerasi sel hati yang lebih baik dibandingkan dengan yang terlihat pada jaringan hati tikus wistar yang tidak diberi ekstrak daun cengkeh.Kata kunci: cengkeh, parasetamol, hati

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