Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Abstract This study evaluated application protocol (etch-and-rinse/ER and self-etching/SE) and dentin wettability (wet and dry) on microtensile bond strength (μTBS) and transdentinal cytotoxicity of ScotchbondTM Universal (SU) adhesive system. The μTBS values and fracture mode were registered 24 h after adhesive system application and resin composite block build-up (n=5). For analysis of transdentinal cytotoxicity, odontoblast-like MDPC-23 cells were seeded on pulpal surface of dentin discs (0.4 mm thick) adapted to artificial pulp chambers (n=8). The adhesive system was applied to occlusal surface, followed by 24-h incubation time. Cell viability (Alamar Blue) and morphology (SEM) were assessed. Adper Single Bond 2 and Clearfil SE Bond were used as positive controls of the ER and SE application protocols, respectively. No treatment was performed on negative control (NC) group. Data were analyzed by ANOVA and Tukey’s tests (α=5%). Higher μTBS values were found for ER mode in comparison with SE protocol (p<0.05). Dentin wettability had no effect on bond strength of SU in both the ER and SE techniques (p>0.05). Most fractures involved hybrid layer and/or adhesive layer. Neither variable prevented the intense toxic effects of adhesive systems on MDPC-23 cultured cells, since intense reduction in cell viability (±88%) and severe alterations in cell morphology were observed for all groups compared to NC, with no differences among them (p>0.05). Therefore, it was concluded that application of SU following the ER protocol had better adhesive performance. However, this adhesive system featured intense transdentinal cytotoxicity to pulp cells, regardless of application protocol and dentin wettability.

Download Full-text

Bond strength of universal adhesive applied to dry and wet dentin

Brazilian Journal of Oral Sciences ◽

10.20396/bjos.v19i0.8658220 ◽

2020 ◽

Vol 19 ◽

pp. e201662

Author(s):

Maria Luísa de Alencar e Silva Leite ◽

Caroline de Farias Charamba ◽

Renally Bezerra Wanderley e Lima ◽

Sônia Saeger Meireles ◽

Rosângela Marques Duarte ◽

...

Keyword(s):

Bond Strength ◽

Storage Time ◽

Resin Composite ◽

Adhesive System ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Bonding Quality ◽

Etch And Rinse ◽

Significant Difference ◽

One Year

Aim: This study evaluated the influence of dentin wettability on the immediate and extended microtensile bond strength (mTBS) of a universal adhesive system used in the etch-and-rinse strategy. Methods: Twenty human third molars were selected and divided into four groups according to the adhesive system and dentin wettability. The mTBS values of each group were registered 24 h and one year after adhesive system application and resin composite block build-up (n=30). Data were analyzed by the t-test (p<0.05). Results: When both adhesive systems were compared, there was no statistically significant difference when they were applied following wet bonding (p>0.05). However, the dry bonding reduced μTBS values of the Adper Single Bond 2 adhesive (p<0.05). Regarding storage time, both groups presented similar μTBS values at 24 h and one year (p>0.05). Conclusions: Therefore, the Scotchbond Universal Adhesive can be applied to dry or wet dentin without compromising the etch-and-rinse bonding quality and the durability of the restorations.

Download Full-text

Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3

Scanning ◽

10.1155/2017/9650420 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Yasmine Mendes Pupo ◽

Cintia Fernanda de Freitas Bernardo ◽

Francielly Fernanda de Freitas A. de Souza ◽

Milton Domingos Michél ◽

Camila Nunes de Morais Ribeiro ◽

...

Keyword(s):

Cell Viability ◽

Culture Medium ◽

Fibroblast Cell ◽

Adhesive System ◽

Negative Control ◽

Adhesive Systems ◽

Universal Adhesive ◽

Glass Cover ◽

Etch And Rinse ◽

Self Etch

The aim of this study was to evaluate in fibroblast cultures the direct cytotoxic effects of etch-and-rinse, self-etch, and universal adhesive systems. The sterile glass cover slips (n=3) were then immersed in culture medium to obtain the eluates for the experimental groups: (1) Adper™ Single Bond 2; (2) Ambar; (3) Adper™ Scotchbond™ Multi-Purpose; (4) Scotchbond™ Universal; (5) Ambar Universal; and (6) OptiBond All-In-One. As a negative control, sterile glass cover slips were immersed in culture medium only. After 24 h, the eluate obtained was applied on fibroblast culture. Cell viability and cell morphology were evaluated by MTT assay and SEM, respectively. Data were analyzed by Kruskal–Wallis and Mann–Whitney tests (α=0.05). All adhesive systems except universal reduced cell viability in 3T3 cells to between 26.04% and 56.57%, and Scotchbond Universal and Ambar Universal reduced cell viability to 2.13% and 3.57%, respectively, when compared to the negative control. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, improvements in universal adhesive system formulations and their mechanisms of action are not accompanied by increased toxicity compared with those in other systems, warranting commitment to the use of these dentin-pulp complexes.

Download Full-text

Immediate Adhesive Properties to Dentin and Enamel of a Universal Adhesive Associated With a Hydrophobic Resin Coat

Operative Dentistry ◽

10.2341/13-203-lr ◽

2014 ◽

Vol 39 (5) ◽

pp. 489-499 ◽

Cited By ~ 38

Author(s):

J Perdigão ◽

MA Muñoz ◽

A Sezinando ◽

IV Luque-Martinez ◽

R Staichak ◽

...

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Hybrid Layer ◽

Adhesive Properties ◽

Universal Adhesive ◽

Etching Technique ◽

Etch And Rinse ◽

One Step ◽

Bond Strengths ◽

Microshear Bond Strength

SUMMARY Objectives To evaluate the effect of acid etching and application of a hydrophobic resin coat on the enamel/dentin bond strengths and degree of conversion (DC) within the hybrid layer of a universal adhesive system (G-Bond Plus [GB]). Methods A total of 60 extracted third molars were divided into four groups for bond-strength testing, according to the adhesive strategy: GB applied as a one-step self-etch adhesive (1-stepSE); GB applied as in 1-stepSE followed by one coat of the hydrophobic resin Heliobond (2-stepSE); GB applied as a two-step etch-and-rinse adhesive (2-stepER); GB applied as in 2-stepER followed by one coat of the hydrophobic resin Heliobond (3-stepER). There were 40 teeth used for enamel microshear bond strength (μSBS) and DC; and 20 teeth used for dentin microtensile bond strength (μTBS) and DC. After restorations were constructed, specimens were stored in water (37°C/24 h) and then tested at 0.5 mm/min (μTBS) or 1.0 mm/min (μSBS). Enamel-resin and dentin-resin interfaces from each group were evaluated for DC using micro-Raman spectroscopy. Data were analyzed with two-way analysis of variance for each substrate and the Tukey test (α=0.05). Results For enamel, the use of a hydrophobic resin coat resulted in statistically significant higher mean enamel μSBS only for the ER strategy (3-stepER vs 2-stepER, p<0.0002). DC was significantly improved for the SE strategy (p<0.00002). For dentin, the use of a hydrophobic resin coat resulted in significantly higher dentin mean μTBS only for the SE strategy (2-stepSE vs 1-stepSE, p<0.0007). DC was significantly improved in groups 2-stepSE and 3-stepER when compared with 1-stepSE and 2-stepER, respectively (p<0.0009). Conclusions The use of a hydrophobic resin coat may be beneficial for the selective enamel etching technique, because it improves bond strengths to enamel when applied with the ER strategy and to dentin when used with the SE adhesion strategy. The application of a hydrophobic resin coat may improve DC in resin-dentin interfaces formed with either the SE or the ER strategy. On enamel, DC may benefit from the application of a hydrophobic resin coat over 1-stepSE adhesives.

Download Full-text

Influence of Chlorhexidine and/or Ethanol Treatment on Bond Strength of an Etch-and-rinse Adhesive to Dentin: An In Vitro and In Situ Study

Operative Dentistry ◽

10.2341/12-486-l ◽

2014 ◽

Vol 39 (1) ◽

pp. 64-71 ◽

Cited By ~ 10

Author(s):

DMS Simões ◽

RT Basting ◽

FLB Amaral ◽

CP Turssi ◽

FMG França

Keyword(s):

Bond Strength ◽

Resin Composite ◽

Adhesive System ◽

Dentin Surface ◽

In Vitro Aging ◽

Etch And Rinse ◽

Bond Interface ◽

Vitro Model

SUMMARY The aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (μTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the μTBS. The in vitro μTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate μTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.

Download Full-text

Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

Brazilian Dental Science ◽

10.14295/bds.2017.v20i4.1461 ◽

2017 ◽

Vol 20 (4) ◽

pp. 55

Author(s):

Rafael Avellar de Carvalho Nunes ◽

Flávia Lucisano Botelho do Amaral ◽

Fabiana Mantovani Gomes França ◽

Cecilia Pedroso Turssi ◽

Roberta Tarkany Basting

Keyword(s):

Bond Strength ◽

Failure Mode ◽

Failure Modes ◽

Resin Composite ◽

Adhesive System ◽

Fracture Pattern ◽

Test Machine ◽

Microtensile Bond Strength ◽

Etch And Rinse ◽

Strength Tests

Objective: the aim of the present study was to evaluate the influence of adding different concentrations of chitosan to an experimental two-step etch-and-rinse adhesive system on the bond strength and failure mode to dentin. Material and Methods: thirty-two flat dentin surfaces were obtained from extracted human third molars and divided into four groups (n=8) for application of the adhesive systems: AD - conventional two-step adhesive system (Adper Single Bond 2); EXP – experimental two-step etch-and-rinse adhesive system; Chi0.2% - EXP with addition of 0.2% Chitosan; Chi0.5% - EXP with addition of 0.5% Chitosan. Resin composite build-ups were made and the composite/dentin specimens were sectioned to obtain rectangular beams with a bond area of approximately 1mm2. After 24 hours, the sticks were submitted to microtensile bond strength tests in a universal test machine. The fracture pattern was evaluated under a stereoscopic loupe at 40X magnification. Results: one-way analysis of variance showed that the type of adhesive system had no significant effect on the bond strength values (p = 0.142), showing the mean bond strength values (standard deviation), in MPa, for the groups as follows: AD=20.1 (5.4); EXP=16.6 (2.3); Chi0.2%=16.1 (2.8); Chi0.5%=16.9 (2.3). In all the groups there was predominance of cohesive fractures in dentin, representing 68 to 82% of the failure modes. Conclusion: the addition of 0.2 or 0.5% of chitosan had no influence on the bond strength and failure mode of an experimental two-step etch-and-rinse adhesive system to dentin.KeywordsChitosan; Dental Adhesives; Failure Mode; Microtensile Bond Strength.

Download Full-text

The effects of different surface contaminators on the shear bond strength of a universal adhesive system to dentin: an experimental study

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.34172/joddd.2021.014 ◽

2021 ◽

Vol 15 (2) ◽

pp. 82-86

Author(s):

Mahmoud Bahari ◽

Siavash Savadi Oskoee ◽

Mohammad Esmaeel Ebrahimi Chaharom ◽

Nasim Molayi

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Adhesive System ◽

Aluminium Chloride ◽

Control Group ◽

Universal Adhesive ◽

Human Teeth ◽

Etch And Rinse ◽

Self Etch ◽

Composite Samples

Background. Contamination of dentin surface is one of the common problems in restorative dentistry. The aim was to investigate the effects of different surface contaminators on the dentin shear bond strength (SBS) of universal adhesive system (UAS) applied in etch-and-rinse (ER) and self-etch (SE) strategies. Methods. One hundred forty-four maxillary anterior sound human teeth were divided into six groups based on the types of surface contaminators: no surface contaminator (control) and experimental groups contaminated with blood, saliva, aluminium chloride (ALC), ferric sulphate (FS), and caries disclosing agent (CDA). Then, each group was further subdivided into two, based on the application strategy of UAS (ER and SE). After applying the adhesive according to the manufacturer’s instructions, and bonding cylindrical composite samples, the SBS was measured. The data were analysed using two-way ANOVA, Tukey’s HSD test and t test (P<0.05). Results. The SBS in all contaminated groups, except for CDA, was significantly lower in both ER and SE strategies compared to control group (P<0.05). A comparison between the application strategies revealed that ER and SE were only significantly different in the FS contaminated group (P<0.05). Conclusion. All tested contaminators, except CDA, significantly decreased SBS of UAS in both ER and SE strategies.

Download Full-text

Interaction morphology and bond strength of nanofilled simplified-step adhesives to acid etched dentin

European Journal of Dentistry ◽

10.1055/s-0039-1698972 ◽

2012 ◽

Vol 06 (04) ◽

pp. 349-360 ◽

Cited By ~ 8

Author(s):

Vinicius Di Hipólito ◽

André Figueiredo Reis ◽

Sumita B Mitra ◽

Mario Fernando de Goes

Keyword(s):

Bond Strength ◽

Colloidal Silica ◽

Resin Composite ◽

Adhesive Layer ◽

Single Bond ◽

Hybrid Layer ◽

Microtensile Bond Strength ◽

Tem Analysis ◽

Cross Sectional ◽

Adhesive Layers

ABSTRACTObjective: To evaluate the effect of nanofillers incorporated into adhesives on the microtensile bond strength (μ-TBS) and interfacial micromorphology to dentin.Methods: The occlusal enamel of 5 human molars was removed and each tooth sectioned into four quarters. The exposed dentin was treated with one of the following adhesives: Adper Single Bond (SB-unfilled), OptiBond Solo Plus (OS-barium aluminoborosilicate, 400nm Ø), Prime & Bond NT (NT-colloidal silica, 7-40 nm Ø) and Adper Single Bond 2 (SB2-colloidal silica, 5nm Ø). Cylinders of resin-based composite were constructed on the adhesive layers. After 24-hour storage, the restored tooth-quadrants were sectioned to obtain stick-shaped specimens (0.8 mm2, cross-sectional area) and submitted to μ-TBS at a cross-speed of 0.5 mm/min. Data were analyzed using one-way ANOVA and Tukey’s test (alpha = .05). Twenty-eight additional teeth were used for interfacial micromorphologic analysis by SEM (16-teeth) and TEM (12-teeth). The dentin surfaces of 32 discs were treated with the adhesives (8 discs for adhesive) and laminated to form disc-pairs using a flowable resin composite for SEM/EDS analysis. For TEM, 90nm-thick nondemineralized unstained sections were processed.Results: SB2 showed significant higher bond strength than SB, OS and NT. The SEM/EDS and TEM analysis revealed nanofillers infiltrated within the interfibrillar spaces of the SB2-hybrid layer. Fillers were concentrated around patent tubular orifices and in the adhesive layer for OS and NT.Conclusion: The presence of nanofillers within the interfibrillar spaces of the SB2-hybrid layer suggests its importance in the improvement of the μ-TBS. (Eur J Dent 2012;6:349-360)

Download Full-text

The Effect of Biodentine Maturation Time on Resin Bond Strength When Aged in Artificial Saliva

International Journal of Dentistry ◽

10.1155/2020/8831813 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Ruba M. Mustafa ◽

Suhad J. Al-Nasrawi ◽

Abtesam I. Aljdaimi

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Artificial Saliva ◽

Adhesive System ◽

Failure Patterns ◽

Etch And Rinse ◽

Significant Difference ◽

Post Hoc ◽

Composite Restoration

Biodentine is a calcium silicate cement (CSC) that has been broadly applied in vital pulp therapy. The quality of the Biodentine-composite bond has a significant effect on the longevity of the definitive restoration. The aim of this study is to investigate the shear bond strength (SBS) between Biodentine and composite restoration at different maturation times of Biodentine aged in artificial saliva. Fifteen Biodentine discs were allocated into three groups (n = 5) based on the timeframe of performance of composite restoration: immediate (after 12 min), after 14 days, and after 28 days of Biodentine maturation. Total etch and rinse adhesive system and bulk-fill regular resin composite were used. The shear bond strength and the failure pattern were assessed. One-way ANOVA with the Bonferroni post hoc test was applied for statistical analysis at p < 0.05 . The highest (32.47 ± 8.18 MPa) and the lowest (4.08 ± 0.81 MPa) SBS values were recorded for 14 days and 12 min groups, respectively. Significant statistical differences were reported among the groups, and a high statistically significant difference was found between the immediate group and the other groups. Adhesive failure patterns were evident in all groups. More clinically acceptable bond strength between the Biodentine and overlaid composite restoration is at 14 days after Biodentine maturation. Delaying the coverage of Biodentine later than 14 days may significantly reduce the SBS. Using the artificial saliva as an aging medium may affect the SBS between Biodentine and composite material.

Download Full-text

Does Bonding Approach Influence the Bond Strength of Universal Adhesive to Dentin of Primary Teeth?

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-41.3.214 ◽

2017 ◽

Vol 41 (3) ◽

pp. 214-218 ◽

Cited By ~ 1

Author(s):

Tathiane Larissa Lenzi ◽

Fabio Zovico Maxnuck Soares ◽

Rachel de Oliveira Rocha

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Primary Tooth ◽

Adhesive Systems ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Etch And Rinse ◽

Tooth Dentin ◽

Self Etch ◽

One Way Anova

Objective: To evaluate the effect of bonding strategy on microtensile bond strength (μTBS) of a new universal adhesive system to primary tooth dentin. Study design: Flat dentin surfaces from 25 primary molars were assigned to 5 groups according to the adhesive and bonding approach: Adper Single Bond 2 (two-step etch-and-rinse adhesive) and Clearfil SE Bond (two-step self-etch system), as controls; Scotchbond Universal Adhesive–self-etch, dry or wet-bonding etch-and-rinse strategies. Composite buildups were constructed and the teeth were sectioned to obtain bonded sticks (0.8 mm2) to be tested under tension at 1mm/min. The μTBS means were analyzed by one-way ANOVA and Tukey's tests (α = 0.05). Failure mode was evaluated using a stereomicroscope (400×). Results: Universal adhesive applied following both dry and wet-bonding etch-and-rinse strategies showed similar bond strength compared with control adhesive systems. Self-etch approach resulted in the lowest μTBS values. For all groups, adhesive/mixed failure prevailed. The percentage of premature debonded specimens was higher when the universal adhesive was used as self-etch mode. Conclusion: The universal adhesive does not share the same versatility of being used in the etch-and-rinse and self-etch approaches; however, the use of the new adhesive following either wet or dry-bonding may be a suitable option as alternative to two-step etch-and-rinse adhesive protocol.

Download Full-text

Effect of Different Adhesion Protocols on the Shear Bond Strength of Universal Adhesive Systems to Sound and Artificial Caries-Affected Dentin

European Journal of General Dentistry ◽

10.1055/s-0041-1732776 ◽

2021 ◽

Author(s):

Omnia M. Sami ◽

Essam A. Naguib ◽

Rasha H. Afifi ◽

Shaymaa M. Nagi

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Failure Modes ◽

Resin Composite ◽

Single Bond ◽

Adhesive Systems ◽

Universal Adhesive ◽

Etch And Rinse ◽

Significant Difference ◽

Self Etch

Abstract Objective Bonding to different dentin substrates influences resin composite restoration outcomes. This study investigated the influence of different adhesion protocols on the shear bond strength of universal adhesive systems to sound and artificial caries-affected dentin (CAD). Materials and Methods Occlusal enamel of 80 premolars were wet grinded to obtain flat midcoronal dentin. Specimens were equally divided according to the substrate condition: sound and CAD by subjecting to pH-cycling for 14 days. Each dentin substrate was bonded with the adhesive systems used in this study: Single Bond universal adhesive or Prime&Bond universal (applied either in etch-and-rinse or self-etch adhesion protocol). Adhesive systems were utilized according to manufacturers’ instructions, then resin composite was built up. Specimens were tested for shear bond strength. The data were analyzed by three-way analysis of variance, and failure modes were determined using stereomicroscope. Results There was no statistically significant difference between the two tested adhesive systems on artificially created CAD with different adhesion protocols. On sound dentin, Single Bond universal, either in the etch-and-rinse or self-etch adhesion protocols, revealed higher statistically significant shear bond strength mean values compared with CAD. Conclusion Single Bond universal adhesive in an etch-and-rinse adhesion protocol improved only bonding to sound dentin, while no added positive effect for the etching step with Prime&Bond universal adhesive was found when bonded to both sound and CAD substrates. The influence of CAD on the performance of the universal adhesives was material-dependent.

Download Full-text