Bond strength of universal adhesive applied to dry and wet dentin

Aim: This study evaluated the influence of dentin wettability on the immediate and extended microtensile bond strength (mTBS) of a universal adhesive system used in the etch-and-rinse strategy. Methods: Twenty human third molars were selected and divided into four groups according to the adhesive system and dentin wettability. The mTBS values of each group were registered 24 h and one year after adhesive system application and resin composite block build-up (n=30). Data were analyzed by the t-test (p<0.05). Results: When both adhesive systems were compared, there was no statistically significant difference when they were applied following wet bonding (p>0.05). However, the dry bonding reduced μTBS values of the Adper Single Bond 2 adhesive (p<0.05). Regarding storage time, both groups presented similar μTBS values at 24 h and one year (p>0.05). Conclusions: Therefore, the Scotchbond Universal Adhesive can be applied to dry or wet dentin without compromising the etch-and-rinse bonding quality and the durability of the restorations.

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Influence of Water Aging on Microtensile Bond Strength of a Flowable and a Packable Bulk-fill Resin Composites to Dentin

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.3652 ◽

2020 ◽

Vol 8 (D) ◽

pp. 112-117

Author(s):

Ayah Atif Selim ◽

Ahmed Fawzy Abo Elezz ◽

Rehab Khalil Safy

Keyword(s):

Bond Strength ◽

Storage Time ◽

Resin Composite ◽

Water Storage ◽

Aging Effect ◽

Mean Value ◽

Control Group ◽

Microtensile Bond Strength ◽

Significant Difference ◽

Influence Of Water

AIM: Investigation of the aging effect on the microtensile bond strength (μTBS) of bulk-fill resin composite (RC) versus a conventionally incrementally applied one. MATERIALS AND METHODS: A total number of 45 sound human impacted third molars extracted molars have been selected to prepare specimens for the μTBS test. Teeth were randomly divided into three groups (C) according to type of RC material which used for restoring the teeth. Where nanohybrid RC (Grandio®SO) was used as the control Group (C1), packable bulk-fill RC (X-tra fil®) was used for restoring teeth in C2 group and flowable bulk-fill RC (X-tra base®) was used for restoring teeth in C3 group. Each group was further subdivided into 3 subgroups (n = 5) according to the water storage time, where in subgroup 1; teeth were stored for 24 h, subgroup 2; teeth were stored for 3 months while for subgroup 3; and teeth were stored for 6 months. After water storage, teeth were sectioned for preparation of μTBS testing beams. Maximum tensile stresses were recorded in megapascal (MPa). RESULTS: After 24 h of water storage, the X-tra base® showed a higher statistically significant μTBS to dentin (33.82 ± 9.84 MPa) than did the other two types of RCs. After 3 months, the X-tra fil® showed the lowest mean value of μTBS (10.90 ± 5.66 MPa), meanwhile, after 6 months of water storage Grandio®SO showed the highest mean value of μTBS (15.85 ± 6.76 MPa). Regardless of the time the X-tra fil® showed the lowest mean of μTBS (15.07 ± 11.73 MPa), while there is no significant difference between the X-tra base® and Grandio®SO. Furthermore, the water aging adversely affects μTBS values which deceased gradually by time. CONCLUSION: The packable bulk-fill RC characterized by lower μTBS to dentin in comparison to the flowable bulk fill and the incrementally applied nanohybrid RCs. Furthermore, the μTBS of the three tested materials decreased gradually by aging.

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Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

Brazilian Dental Science ◽

10.14295/bds.2017.v20i4.1461 ◽

2017 ◽

Vol 20 (4) ◽

pp. 55

Author(s):

Rafael Avellar de Carvalho Nunes ◽

Flávia Lucisano Botelho do Amaral ◽

Fabiana Mantovani Gomes França ◽

Cecilia Pedroso Turssi ◽

Roberta Tarkany Basting

Keyword(s):

Bond Strength ◽

Failure Mode ◽

Failure Modes ◽

Resin Composite ◽

Adhesive System ◽

Fracture Pattern ◽

Test Machine ◽

Microtensile Bond Strength ◽

Etch And Rinse ◽

Strength Tests

Objective: the aim of the present study was to evaluate the influence of adding different concentrations of chitosan to an experimental two-step etch-and-rinse adhesive system on the bond strength and failure mode to dentin. Material and Methods: thirty-two flat dentin surfaces were obtained from extracted human third molars and divided into four groups (n=8) for application of the adhesive systems: AD - conventional two-step adhesive system (Adper Single Bond 2); EXP – experimental two-step etch-and-rinse adhesive system; Chi0.2% - EXP with addition of 0.2% Chitosan; Chi0.5% - EXP with addition of 0.5% Chitosan. Resin composite build-ups were made and the composite/dentin specimens were sectioned to obtain rectangular beams with a bond area of approximately 1mm2. After 24 hours, the sticks were submitted to microtensile bond strength tests in a universal test machine. The fracture pattern was evaluated under a stereoscopic loupe at 40X magnification. Results: one-way analysis of variance showed that the type of adhesive system had no significant effect on the bond strength values (p = 0.142), showing the mean bond strength values (standard deviation), in MPa, for the groups as follows: AD=20.1 (5.4); EXP=16.6 (2.3); Chi0.2%=16.1 (2.8); Chi0.5%=16.9 (2.3). In all the groups there was predominance of cohesive fractures in dentin, representing 68 to 82% of the failure modes. Conclusion: the addition of 0.2 or 0.5% of chitosan had no influence on the bond strength and failure mode of an experimental two-step etch-and-rinse adhesive system to dentin.KeywordsChitosan; Dental Adhesives; Failure Mode; Microtensile Bond Strength.

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The Effect of Biodentine Maturation Time on Resin Bond Strength When Aged in Artificial Saliva

International Journal of Dentistry ◽

10.1155/2020/8831813 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Ruba M. Mustafa ◽

Suhad J. Al-Nasrawi ◽

Abtesam I. Aljdaimi

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Artificial Saliva ◽

Adhesive System ◽

Failure Patterns ◽

Etch And Rinse ◽

Significant Difference ◽

Post Hoc ◽

Composite Restoration

Biodentine is a calcium silicate cement (CSC) that has been broadly applied in vital pulp therapy. The quality of the Biodentine-composite bond has a significant effect on the longevity of the definitive restoration. The aim of this study is to investigate the shear bond strength (SBS) between Biodentine and composite restoration at different maturation times of Biodentine aged in artificial saliva. Fifteen Biodentine discs were allocated into three groups (n = 5) based on the timeframe of performance of composite restoration: immediate (after 12 min), after 14 days, and after 28 days of Biodentine maturation. Total etch and rinse adhesive system and bulk-fill regular resin composite were used. The shear bond strength and the failure pattern were assessed. One-way ANOVA with the Bonferroni post hoc test was applied for statistical analysis at p < 0.05 . The highest (32.47 ± 8.18 MPa) and the lowest (4.08 ± 0.81 MPa) SBS values were recorded for 14 days and 12 min groups, respectively. Significant statistical differences were reported among the groups, and a high statistically significant difference was found between the immediate group and the other groups. Adhesive failure patterns were evident in all groups. More clinically acceptable bond strength between the Biodentine and overlaid composite restoration is at 14 days after Biodentine maturation. Delaying the coverage of Biodentine later than 14 days may significantly reduce the SBS. Using the artificial saliva as an aging medium may affect the SBS between Biodentine and composite material.

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Does Bonding Approach Influence the Bond Strength of Universal Adhesive to Dentin of Primary Teeth?

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-41.3.214 ◽

2017 ◽

Vol 41 (3) ◽

pp. 214-218 ◽

Cited By ~ 1

Author(s):

Tathiane Larissa Lenzi ◽

Fabio Zovico Maxnuck Soares ◽

Rachel de Oliveira Rocha

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Primary Tooth ◽

Adhesive Systems ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Etch And Rinse ◽

Tooth Dentin ◽

Self Etch ◽

One Way Anova

Objective: To evaluate the effect of bonding strategy on microtensile bond strength (μTBS) of a new universal adhesive system to primary tooth dentin. Study design: Flat dentin surfaces from 25 primary molars were assigned to 5 groups according to the adhesive and bonding approach: Adper Single Bond 2 (two-step etch-and-rinse adhesive) and Clearfil SE Bond (two-step self-etch system), as controls; Scotchbond Universal Adhesive–self-etch, dry or wet-bonding etch-and-rinse strategies. Composite buildups were constructed and the teeth were sectioned to obtain bonded sticks (0.8 mm2) to be tested under tension at 1mm/min. The μTBS means were analyzed by one-way ANOVA and Tukey's tests (α = 0.05). Failure mode was evaluated using a stereomicroscope (400×). Results: Universal adhesive applied following both dry and wet-bonding etch-and-rinse strategies showed similar bond strength compared with control adhesive systems. Self-etch approach resulted in the lowest μTBS values. For all groups, adhesive/mixed failure prevailed. The percentage of premature debonded specimens was higher when the universal adhesive was used as self-etch mode. Conclusion: The universal adhesive does not share the same versatility of being used in the etch-and-rinse and self-etch approaches; however, the use of the new adhesive following either wet or dry-bonding may be a suitable option as alternative to two-step etch-and-rinse adhesive protocol.

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Effect of Silver Diamine Fluoride on Microtensile Bond Strength to Dentin

Operative Dentistry ◽

10.2341/11-344-l ◽

2012 ◽

Vol 37 (6) ◽

pp. 610-616 ◽

Cited By ~ 26

Author(s):

RL Quock ◽

JA Barros ◽

SW Yang ◽

SA Patel

Keyword(s):

Bond Strength ◽

Resin Composite ◽

Bonding Agent ◽

Microtensile Bond Strength ◽

Silver Diamine Fluoride ◽

Microtensile Testing ◽

Etch And Rinse ◽

Significant Difference ◽

Diamond Saw ◽

Self Etch

SUMMARY The aim of this in vitro study was to investigate the effect of the cariostatic and preventive agent silver diamine fluoride (SDF) on the microtensile bond strength of resin composite to dentin. Forty-two caries-free, extracted molars were flattened occlusally and apically using a diamond saw, and the exposed occlusal dentin was polished with a series of silicon carbide papers, all under water irrigation. The teeth were then randomly divided into six groups of seven teeth each that were treated as follows: 1) Peak SE self-etch bonding agent; 2) 12% SDF + Peak SE; 3) 38% SDF + Peak SE; 4) Peak LC etch-and-rinse bonding agent; 5) 12% SDF + Peak LC; and 6) 38% SDF + Peak LC. Four-millimeter buildups of Amelogen Plus were incrementally placed on all teeth; after a 24-hour storage period in distilled water, the specimens were sectioned perpendicular to the adhesive interface to produce beams of cross-sectional surface area measuring approximately 1 mm2. The beams were placed on a microtensile testing machine, which utilized a single-speed pump motor and force gauge at 20 kgf × 0.01 second to record maximum tensile force before failure occurred. Two-way analysis of variance and post hoc Tukey tests were performed to compare the effects of the SDF on microtensile bond strength, with statistical significance set at α = 0.05. None of the experimental groups treated with different concentrations of SDF showed a significant difference in bond strength compared to the control groups, and there was no significant difference in bond strength between self-etch and etch-and-rinse groups. However, the effect of SDF on self-etch bonded teeth compared to etch-and-rinse bonded teeth was statistically significant (p=0.0363), specifically at the 12% concentration. SDF does not adversely affect the bond strength of resin composite to noncarious dentin.

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Effect of Different Adhesion Protocols on the Shear Bond Strength of Universal Adhesive Systems to Sound and Artificial Caries-Affected Dentin

European Journal of General Dentistry ◽

10.1055/s-0041-1732776 ◽

2021 ◽

Author(s):

Omnia M. Sami ◽

Essam A. Naguib ◽

Rasha H. Afifi ◽

Shaymaa M. Nagi

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Failure Modes ◽

Resin Composite ◽

Single Bond ◽

Adhesive Systems ◽

Universal Adhesive ◽

Etch And Rinse ◽

Significant Difference ◽

Self Etch

Abstract Objective Bonding to different dentin substrates influences resin composite restoration outcomes. This study investigated the influence of different adhesion protocols on the shear bond strength of universal adhesive systems to sound and artificial caries-affected dentin (CAD). Materials and Methods Occlusal enamel of 80 premolars were wet grinded to obtain flat midcoronal dentin. Specimens were equally divided according to the substrate condition: sound and CAD by subjecting to pH-cycling for 14 days. Each dentin substrate was bonded with the adhesive systems used in this study: Single Bond universal adhesive or Prime&Bond universal (applied either in etch-and-rinse or self-etch adhesion protocol). Adhesive systems were utilized according to manufacturers’ instructions, then resin composite was built up. Specimens were tested for shear bond strength. The data were analyzed by three-way analysis of variance, and failure modes were determined using stereomicroscope. Results There was no statistically significant difference between the two tested adhesive systems on artificially created CAD with different adhesion protocols. On sound dentin, Single Bond universal, either in the etch-and-rinse or self-etch adhesion protocols, revealed higher statistically significant shear bond strength mean values compared with CAD. Conclusion Single Bond universal adhesive in an etch-and-rinse adhesion protocol improved only bonding to sound dentin, while no added positive effect for the etching step with Prime&Bond universal adhesive was found when bonded to both sound and CAD substrates. The influence of CAD on the performance of the universal adhesives was material-dependent.

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Bond Strength and Cytotoxicity of a Universal Adhesive According to the Hybridization Strategies to Dentin

Brazilian Dental Journal ◽

10.1590/0103-6440201801698 ◽

2018 ◽

Vol 29 (1) ◽

pp. 68-75 ◽

Cited By ~ 6

Author(s):

Maria Luísa de Alencar e Silva Leite ◽

Carlos Alberto de Souza Costa ◽

Rosângela Marques Duarte ◽

Ana Karina Maciel de Andrade ◽

Diana Gabriela Soares

Keyword(s):

Bond Strength ◽

Cell Viability ◽

Cultured Cells ◽

Resin Composite ◽

Adhesive Layer ◽

Adhesive System ◽

Negative Control ◽

Hybrid Layer ◽

Universal Adhesive ◽

Etch And Rinse

Abstract This study evaluated application protocol (etch-and-rinse/ER and self-etching/SE) and dentin wettability (wet and dry) on microtensile bond strength (μTBS) and transdentinal cytotoxicity of ScotchbondTM Universal (SU) adhesive system. The μTBS values and fracture mode were registered 24 h after adhesive system application and resin composite block build-up (n=5). For analysis of transdentinal cytotoxicity, odontoblast-like MDPC-23 cells were seeded on pulpal surface of dentin discs (0.4 mm thick) adapted to artificial pulp chambers (n=8). The adhesive system was applied to occlusal surface, followed by 24-h incubation time. Cell viability (Alamar Blue) and morphology (SEM) were assessed. Adper Single Bond 2 and Clearfil SE Bond were used as positive controls of the ER and SE application protocols, respectively. No treatment was performed on negative control (NC) group. Data were analyzed by ANOVA and Tukey’s tests (α=5%). Higher μTBS values were found for ER mode in comparison with SE protocol (p<0.05). Dentin wettability had no effect on bond strength of SU in both the ER and SE techniques (p>0.05). Most fractures involved hybrid layer and/or adhesive layer. Neither variable prevented the intense toxic effects of adhesive systems on MDPC-23 cultured cells, since intense reduction in cell viability (±88%) and severe alterations in cell morphology were observed for all groups compared to NC, with no differences among them (p>0.05). Therefore, it was concluded that application of SU following the ER protocol had better adhesive performance. However, this adhesive system featured intense transdentinal cytotoxicity to pulp cells, regardless of application protocol and dentin wettability.

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Evaluation of Microtensile Bond Strength of Universal Self-etch Adhesive System to Wet and Dry Dentin

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.4109 ◽

2020 ◽

Vol 8 (D) ◽

pp. 77-81

Author(s):

Mohamed Ahmed Wakwak ◽

Eslam Hassan Gabr ◽

Ahmed Mohamed Elmarakby

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Solvent Free ◽

Microtensile Bond Strength ◽

Universal Adhesive ◽

Significance Level ◽

Significant Difference ◽

One Step ◽

Storage Times ◽

Self Etch

BACKGROUND: The durability of dentin-resin interfaces with the universal adhesive system is a crucial characteristic with chemical interactions between the exposed collagen and the adhesive monomers, but it is still compromised with wet and dry mode. AIM: The present study evaluated the effect of dentin wetness and solvents containing of one-step self-etch adhesives on the microtensile bond strength (μTBS) of dentin at different storage times. METHODS: Occlusal dentin of 54 extracted human molars was exposed. Each adhesive agent was applied according to manufacturer instructions to wet and dry dentin surfaces. Composite resin was incrementally built up. Bond strengths to dentin were determined using the μTBS test after water storage for 24 h, 1 month, and 6 months. One-way ANOVA was used to compare between more than two non-related samples. The significance level was set at p ≤ 0.05. RESULTS: Dryness of dentin increases the μTBS with solvent-containing adhesives while decrease the μTBS with solvent-free adhesive. There was an increase in microtensile bond strength values in the case of ethanol water-based self-etch adhesive over time. No statistically significant difference was found among different storage times regarding μTBS for solvent-free adhesive, while a statistically significant difference was found among different storage times in μTBS for solvent-containing adhesives. CONCLUSION: Universal adhesive systems improve the durability and stability of dentin bond strength.

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Effect Of Chitosan Treatment On Shear Bond Strength Of Composite To Deep Dentin Using Self-Etch And Total-Etch Adhesive Systems

Brazilian Dental Science ◽

10.14295/bds.2021.v24i2.2440 ◽

2021 ◽

Vol 24 (2) ◽

Author(s):

Fateme Vasei ◽

Farahnaz Sharafeddin

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Resin Composite ◽

Testing Machine ◽

Adhesive System ◽

Single Bond ◽

Adhesive Systems ◽

Etch And Rinse ◽

Significant Difference ◽

Self Etch

Objective: To assess the shear bond strength (SBS) of resin composite to deep dentin, using 1 and 2.5% chitosan pretreatment as well as different adhesive systems. Material and Methods: 80 human maxillary molars were randomly divided to eight groups according to the type of adhesive system and dentin pretreatment (n = 10): I) two-step self-etch system (Clearfil SE bond); II) two-step etch-andrinse system (Adper single bond 2); III) 2.5% chitosan + Clearfil SE bond; IV) 2.5% chitosan +etch + Adper single bond 2; V) etch + 2.5% chitosan + Adper single bond 2; VI) 1% chitosan + Clearfil SE bond; VII) 1% chitosan + etch + Adper single bond 2; VIII) etch + 1% chitosan + Adper single bond 2 (chitosan solution (w/v): 2.5 g and 1 g of chitosan (Sigma Aldrich, USA) was dissolved in 100 ml of 1% acetic acid). Plastic molds were positioned on dentin and filled with composite (Z350, 3M ESPE, USA). SBS (MPa) was tested using a universal testing machine. ANOVA tests, Tukey’s test, and independent t test were used to analyze data (p < 0.05). Results: The highest SBS value among self-etch groups was observed with 1% chitosan (p = 0.001). In the etch-and-rinse group, the SBS of 1% chitosan was significantly lower than the other groups. Chitosan treatment following acid etching led to higher SBS in comparison to when chitosan was applied before etching, with the significant difference in 1% concentration (p = 0.030). A predominance of mix fractures was observed in dentin. Conclusion: Improved dentin bond strength can be achieved through immediate dentin pretreatment with 1% chitosan in self-etch adhesive systems. Chitosan Pretreatment may not be advantageous for etch-and-rinse adhesive systems. Keywords Adhesive system; Chitosan; Deep dentin; Shear strength.

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Influence of a heating device and adhesive temperature on bond strength of a simplified ethanol-based adhesive system

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.07315 ◽

2016 ◽

Vol 45 (2) ◽

pp. 97-102 ◽

Cited By ~ 2

Author(s):

Marcos Paulo Marchiori CARVALHO ◽

Rachel de Oliveira ROCHA ◽

Ivo KREJCI ◽

Tissiana BORTOLOTTO ◽

Fábio Ecke BISOGNO ◽

...

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Microtensile Bond Strength ◽

Air Jet ◽

Etch And Rinse ◽

Significant Difference ◽

The Mean ◽

Acid Gel ◽

One Way Anova ◽

Warming Device

Abstract Introduction Increased adhesive temperature has been reported to promote solvent evaporation, reduce viscosity, and improve monomeric permeation into dentin. Objective The aim of this study was to determine the influence of different heating methods on the microtensile bond strength of an etch-and-rinse adhesive to dentin. Material and method Twenty-four caries-free extracted human third molars were transversally sectioned to expose a flat dentinal surface. The samples were etched with 37% phosphoric acid gel and divided into three groups (n = 8): 1) Control - the adhesive system (Adper Single Bond 2; 3M ESPE) was applied at room temperature; 2) Warming device - the adhesive was warmed to 37°C in a custom device before application; and 3) Warm air - the adhesive was warmed to 50°C with an air jet after application on dentin. The specimens were restored with a composite resin (Filtek Z250 A2, 3M ESPE) and prepared for microtensile bond strength testing, after 24 h in water storage. The data were subjected to one-way ANOVA and Tukey's test (p < 0.05). Result There was no significant difference among the groups (p > 0.05). The mean bond strength values in the control, the warming device, and the warm air groups were 48.5 (± 5.2), 40.35 (± 4.9), and 47.2 (± 5.3) MPa, respectively (p = 0.05). Conclusion The different heating methods had no significant influence on the immediate microtensile bond strength of an etch-and-rinse ethanol-based adhesive to dentin.

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