scholarly journals Evaluation of the WaveOne Gold and One Shape New Generation in Reducing Enterococcus faecalis from Root Canal

2018 ◽  
Vol 29 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Raquel Esmeralda Guillén ◽  
Cleber Keiti Nabeshima ◽  
Hector Caballero-Flores ◽  
Miguel Roig Cayón ◽  
Montse Mercadé ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
File Systems ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Single File ◽  
Bacterial Removal ◽  
Significant Difference ◽  
Post Hoc ◽  
New Generation

Abstract The aim of this study was to evaluate the WaveOne Gold and One Shape New Generation systems regarding the bacterial removal from root canals infected with Enterococcus faecalis by comparing them to the conventional WaveOne and One Shape systems. Forty-eight distobuccal root canals of maxillary molars sterilized with ethylene oxide were infected with E. faecalis for 21 days, and then root canal initial bacterial sample was collected with paper cones and plated on M-enterococcus agar. The specimens were randomly divided into 4 groups according to the instrumentation: WaveOne Gold, One Shape New Generation, WaveOne and One Shape. After instrumentation, samples were collected with use of scraping and paper cones at immediate and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by Friedman test and intergroup analysis by Kruskal-Wallis with Dunn’s post-hoc test, all at 5% significance. All techniques significantly reduced the number of bacteria in the root canal (p<0.05). WaveOne Gold and One Shape New Generation promoted higher bacterial reduction than WaveOne and One Shape systems (p<0.05), but no significant difference was found between WaveOne Gold and One Shape New Generation or between WaveOne and One Shape (p>0.05). Novel single-file systems promote better bacterial removal than the conventional single-file systems.

scholarly journals Effectivity of convex triangular and rectangular cross section instruments on decreasing the amount of root canal enterococcus faecalis bacteria colonies

2016 ◽  
Vol 1 (2) ◽  
pp. 120
Author(s):  
Meilina Goenawan ◽  
Juni J. Nugroho ◽  
Christine R. Anastasia ◽  
Aries C. Trilaksana ◽  
Nurhayaty Natsir ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Cross Section ◽  
Root Canal ◽  
Rectangular Cross Section ◽  
Bacterial Count ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Triangular Cross Section ◽  
Rotary Instruments ◽  
Significant Difference

This study aims to evaluate effectiveness difference between convex triangular and rectangular cross section rotary  instruments on root canal Enterococcus faecalis bacteria colonies. Samples mandibular premolar teeth with one narrow and straight root canal divided into 3 groups : instrumented with convex triangular cross section rotary instruments, rectangular cross section rotary instruments, and convex triangular cross section manual  instruments. Each group consisted of 9 samples. Standardized teeth length, instrumented to #15 K-file  and sterilized. E. faecalis suspension in BHIB was standardized then 27 root canals contaminated. Root canals were incubated for 21 days. Every 48 hours root canals added with BHIB. Initial samples were collected, plated in agar culture medium and incubated for bacterial count. and then bacterial count was performed again. Data were collected and analysed using Paired T-test and ANOVAThe results showed there are significant  difference on Enterococcus faecalis bacteria colonies amount before and after preparation with all instruments that was used and bacterial reduction amount in root canals were greater on convex triangular cross section instruments than on rectangular cross section instruments even there was no significant difference.

scholarly journals Bacterial removal in flattened root canals with different tapers of ProDesign Logic single-file system

2021 ◽  
Vol 10 (9) ◽  
pp. e5910917701
Author(s):  
Vanessa Sandini ◽  
Viviane Godoy ◽  
Marina Carvalho Prado ◽  
Ricardo Ferreira ◽  
Adriana De-Jesus-Soares ◽  
...  
Keyword(s):  
Bacterial Biofilm ◽  
Bacterial Reduction ◽  
Sterile Saline ◽  
Root Canals ◽  
Bacterial Removal ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Level Of Significance ◽  
Post Hoc

Objective: This study evaluated the bacterial removal in flattened root canals after preparation with different tapers of ProDesign Logic instruments. Methodology: Fifty-five mandibular incisors with flattened root canals were contaminated with a pure culture of Enterococcus faecalis ATCC 29212 for 21 days at 37ºC. Root canals preparation was performed using ProDesign Logic and 0.9% sterile saline solution for irrigation. The specimens were randomly allocated to three experimental groups (n = 15) according to the taper of the instrument used: 25.03, 25.04, or 25.06. Intracanal bacteriological samples were taken before (S1) and after (S2) preparation and the number of colony-forming units per milliliter (CFU/mL) was calculated. Differences in bacterial counts before and after treatment, within each group, were analyzed by the paired t-test. Groups were compared by ANOVA with a post hoc test. The level of significance was set at p <0.05. Results: Mature biofilm formation was confirmed at root canal walls by scanning electron microscopy. Bacterial colonies were significantly reduced in all experimental groups at S2 compared to S1 (p <0.001). No significant difference was observed between groups regarding the percentage of bacterial reduction (p >0.05). Conclusion: Preparation of flattened root canals using ProDesign Logic instruments with tapers of 0.03, 0.04 and 0.06 resulted in similar planktonic and bacterial biofilm reduction. None of the instruments rendered root canals completely free of bacteria.

scholarly journals Effectivity of convex triangular and rectangular cross section instruments on decreasing the amount of root canal Enterococcus faecalis bacteria colonies

2016 ◽  
Vol 1 (2) ◽  
pp. 264
Author(s):  
Meilina Goenawan ◽  
Juni Jekti Nugroho ◽  
Christine Anastasia Rovani ◽  
Aries Chandra Trilaksana ◽  
Nurhayaty Natsir ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Cross Section ◽  
Root Canal ◽  
Rectangular Cross Section ◽  
Bacterial Count ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Triangular Cross Section ◽  
Rotary Instruments ◽  
Significant Difference

<div class="WordSection1"><p>this study aims to evaluate effectiveness difference between convex triangular and rectangular cross section rotary  instruments on root canal Enterococcus faecalis bacteria colonies. Samples mandibular premolar teeth with one narrow and straight root canal divided into 3 groups : instrumented with convex triangular cross section rotary instruments, rectangular cross section rotary instruments, and convex triangular cross section manual  instruments. Each group consisted of 9 samples. Standardized teeth length, instrumented to #15 K-file  and sterilized. E. faecalis suspension in BHIB was standardized then 27 root canals contaminated. Root canals were incubated for 21 days. Every 48 hours root canals added with BHIB. Initial samples were collected, plated in agar culture medium and incubated for bacterial count. and then bacterial count was performed again. Data were collected and analysed using Paired T-test and ANOVA</p><p>The results showed there are significant  difference on Enterococcus faecalis bacteria colonies amount before and after preparation with all instruments that was used and bacterial reduction amount in root canals were greater on convex triangular cross section instruments than on rectangular cross section instruments even there was no significant difference.</p></div>

scholarly journals Quantitative evaluation of apically extruded debris during root canal instrumentation with ProTaper Universal, ProTaper Next, WaveOne, and self-adjusting file systems

2014 ◽  
Vol 08 (04) ◽  
pp. 504-508 ◽  
Author(s):  
Damla Ozsu ◽  
Ertugrul Karatas ◽  
Hakan Arslan ◽  
Meltem C. Topcu
Keyword(s):  
Root Canal ◽  
File Systems ◽  
Universal Group ◽  
Significance Level ◽  
Root Canals ◽  
Single File ◽  
Measurable Amount ◽  
Significant Difference ◽  
The One ◽  
Root Canal Instrumentation

ABSTRACT Objectives: The aim of this study was to compare the amount of apically extruded debris during preparation with ProTaper Universal (Dentsply Maillefer, Ballaigues, Switzerland), ProTaper Next (Dentsply Maillefer), a reciprocating single-file (WaveOne; VDW GmbH, Munich, Germany), and a self-adjusting file (SAF; ReDent Nova, Ra'anna, Israel). Materials and Methods: Fifty-six intact mandibular premolar teeth were randomly assigned to four groups. The root canals were prepared according to the manufacturers’ instructions using the ProTaper Universal, ProTaper Next, WaveOne, and SAF. Apically extruded debris was collected in preweighted Eppendorf tubes during instrumentation. The net weight of the apically extruded debris was determined by subtracting the preweights and postweights of the tubes. The data were statistically analyzed using the one-way analysis of variance and the least significant difference tests at a significance level of P < 0.05. Results: A measurable amount of debris was apically extruded in all groups, and the amounts of debris extrusion in the groups were statistically significant (P < 0.001). The ProTaper Next and WaveOne groups resulted in less debris extrusion than the ProTaper Universal group (P < 0.05), and the SAF group resulted in the least debris extrusion. Conclusions: Within the limitations of the present study, it can be concluded that all systems extruded debris beyond the apical foramen.

scholarly journals Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

2018 ◽  
Vol 29 (5) ◽  
pp. 459-464 ◽  
Author(s):  
Larissa Tais Soligo ◽  
Ediléia Lodi ◽  
Ana Paula Farina ◽  
Matheus Albino Souza ◽  
Cristina de Mattos Pimenta Vidal ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Enterococcus Faecalis ◽  
Grape Seed ◽  
Control Group ◽  
Bacterial Reduction ◽  
Calcium Hypochlorite ◽  
Root Canals ◽  
Colony Forming Units ◽  
Before And After ◽  
Post Hoc

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

scholarly journals Efficiency of the Self Adjusting File, WaveOne, Reciproc, ProTaper and hand files in root canal debridement

2014 ◽  
Vol 08 (03) ◽  
pp. 326-329 ◽  
Author(s):  
K. Meltem Topcu ◽  
Ertugrul Karatas ◽  
Damla Ozsu ◽  
Ibrahim Ersoy
Keyword(s):  
Contrast Medium ◽  
Root Canal ◽  
File Systems ◽  
The Self ◽  
Root Canals ◽  
Single File ◽  
Canal Systems ◽  
Better Than

ABSTRACT Objectives: The aim of this study was to compare the canal debridement capabilities of three single file systems, ProTaper, and K-files in oval-shaped canals. Materials and Methods: Seventy-five extracted human mandibular central incisors with oval-shaped root canals were selected. A radiopaque contrast medium (Metapex; Meta Biomed Co. Ltd., Chungcheongbuk-do, Korea) was introduced into the canal systems and the self-adjusting file (SAF), WaveOne, Reciproc, ProTaper, and K-files were used for the instrumentation of the canals. The percentage of removed contrast medium was calculated using pre- and post-operative radiographs. Results: An overall comparison between the groups revealed that the hand file (HF) and SAF groups presented the lowest percentage of removed contrast medium, whereas the WaveOne group showed the highest percentage (P < 0.001). The ProTaper group removed more contrast medium than the SAF and HF groups (P < 0.05). Conclusions: None of the instruments was able to remove the contrast medium completely. WaveOne performed significantly better than other groups.

scholarly journals Extrusion of Debris from Primary Molar Root Canals following Instrumentation with Traditional and New File Systems

2017 ◽  
Vol 18 (11) ◽  
pp. 1040-1044 ◽  
Author(s):  
Bhagyashree Thakur ◽  
Ajinkya M Pawar ◽  
Anda Kfir ◽  
Prasanna Neelakantan
Keyword(s):  
Primary Teeth ◽  
File Systems ◽  
Primary Molar ◽  
Group Iii ◽  
Root Canals ◽  
Group I ◽  
Significant Difference ◽  
Molar Teeth ◽  
Post Hoc ◽  
Root Canal Instrumentation

ABSTRACT Introduction To assess the amount of debris extruded apically during instrumentation of distal canals of extracted primary molars by three instrument systems [ProTaper Universal (PTU), ProTaper NEXT (PTN), and self-adjusting file (SAF)] compared with conventional stainless steel hand K-files (HF, control). Materials and methods Primary mandibular molars (n = 120) with a single distal canal were selected and randomly divided into four groups (n = 30) for root canal instrumentation using group I, HF (to size 0.30/0.02 taper), group II, PTU (to size F3), group III, PTN (to size X3), and group IV, SAF. Debris extruded during instrumentation was collected in preweighed Eppendorf tubes, stored in an incubator at 70°C for 5 days and then weighed. Statistical analysis was performed by one-way analysis of variance (ANOVA), followed by Turkey's post hoc test (p = 0.05). Results All the groups resulted in extrusion of debris. There was statistically significant difference (p < 0.001) in the debris extrusion between the three groups: HF (0.00133 ± 0.00012), PTU (0.00109 ± 0.00005), PTN (0.00052 ± 0.00008), and SAF (0.00026 ± 0.00004). Conclusion Instrumentation with SAF resulted in the least debris extrusion when used for shaping root canals of primary molar teeth. Clinical significance Debris extrusion in primary teeth poses an adverse effect on the stem cells and may also alter the permanent dental germ. Debris extrusion is rarely reported for primary teeth and it is important for the clinician to know which endodontic instrumentation leads to less extrusion of debris. How to cite this article Thakur B, Pawar AM, Kfir A, Neelakantan P. Extrusion of Debris from Primary Molar Root Canals following Instrumentation with Traditional and New File Systems. J Contemp Dent Pract 2017;18(11):1040-1044.

scholarly journals Evaluation of Cyclic Fatigue of Hyflex EDM, Twisted Files, and ProTaper Gold Manufactured with Different Processes: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Pooja D. Khandagale ◽  
Prashant P. Shetty ◽  
Saleem D. Makandar ◽  
Pradeep A. Bapna ◽  
Mohmed Isaqali Karobari ◽  
...  
Keyword(s):  
Fatigue Resistance ◽  
Root Canal ◽  
File Systems ◽  
In Vitro Study ◽  
Cyclic Fatigue ◽  
Segment Length ◽  
Significant Difference ◽  
Number Of Cycles ◽  
Post Hoc

Introduction. The main aims of root canal instrumentation are to provide an environment that will lead to healing and to provide a root canal shape that is comfortable to clean and seal. When working with rotary endodontic instruments, the most significant concerns are that the instrument might fracture in the root canal, thus affecting the treatment outcome. Hence, it is of immense importance to know which file systems have more cyclic fatigue resistance. Methodology. This study evaluated the effect of the curved segment length of the artificial canal (the arch), and the number of cycles necessary in fracture of Hyflex EDM, Twisted files, and ProTaper Gold were recorded. Sixty NiTi rotary instruments of 25 mm length (Hyflex EDM (20), Twisted files (20), and ProTaper Gold (20)) were tested in a metal block with simulated canal having 90° angle of curvature. The study was performed with a specific radius and degree of curvature, i.e., 8 mm radius and 90⁰ angle of curvature, and data obtained were subsequently subjected to statistical evaluation using one-way analysis of variance and Tukey’s post hoc test. Result. The Hyflex EDM (774.29) exhibited the maximum cyclic fatigue resistance compared to Twisted files (654.875) and ProTaper Gold (375.575). A statistically significant difference was observed between the tested groups. Conclusion. The Hyflex EDM files showed the highest cyclic fatigue resistance, followed by Twisted files and ProTaper Gold files.

scholarly journals Evaluation of Apically Extruded Debris and Irrigant Produced by Different Nickel-Titanium Instrument Systems in Primary Teeth

2015 ◽  
Vol 16 (11) ◽  
pp. 864-868 ◽  
Author(s):  
Ebru Kucukyilmaz ◽  
Selcuk Savas ◽  
Gokhan Saygili ◽  
Banu Uysal
Keyword(s):  
Primary Teeth ◽  
File System ◽  
File Systems ◽  
Nickel Titanium ◽  
Root Canals ◽  
System A ◽  
Bonferroni Adjustment ◽  
Single File ◽  
Significant Difference ◽  
Irrigation Solution

ABSTRACT Aim The purpose of this study was to determine the amount of extruded debris and irrigant associated with different single-file systems and one multiple-file system. Materials and methods Forty-five newly extracted single rooted primary canine teeth were used in this study. The root canals were instrumented using the reciprocating single-file system, the single-file rotary system and the multiple-file rotary system. A 10–6 precision micro-balance was used to calculate the amount of extruded debris and irrigant. The incubation period was set as 15 days at 37°C to obtain dry debris. The preparation time for instrumentation was also recorded. The data were statistically analyzed by Manova and Bonferroni adjustment. Results Considering the apically extruded debris, while there was statistically significant difference between Reciproc and OneShape groups (p < 0.05), no statistically significant difference was found among the other groups (p > 0.05). In terms of irrigation solution, although significant difference was obtained between the Reciproc and other two groups (p < 0.05), no statistically significant difference was obtained between the two rotary instruments (p > 0.05). Instrumentation was significantly faster using Reciproc than with all other instrument (p < 0.05). Conclusion All systems caused apical debris and irrigant extrusion. Full-sequences rotary instrumentation systems may be preferable for preparation primary teeth. Clinical significance This is the first study carrying out to compare both apical debris and irrigation solution extrusion with reciprocal and rotary single-file systems with other preparation systems in primary teeth. How to cite this article Kucukyilmaz E, Savas S, Saygili G, Uysal B. Evaluation of Apically Extruded Debris and Irrigant Produced by Different Nickel-Titanium Instrument Systems in Primary Teeth. J Contemp Dent Pract 2015;16(11):864-868.

scholarly journals Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study

2017 ◽  
Vol 18 (3) ◽  
pp. 214-217
Author(s):  
Vinisha Pandey ◽  
Harsh Priyank ◽  
Abhishek Bagul ◽  
Kishore Kumar Majety ◽  
Parul Verma ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Saline Solution ◽  
Brain Heart Infusion ◽  
Root Canals ◽  
Irrigation Methods ◽  
Group I ◽  
Significant Difference

ABSTRACT Introduction Endodontic treatment removes all pathogens, such as Enterococcus faecalis from pulp and root canals. The aim of this study is to assess the usefulness of sodium hypochlorite (NaOCl) in removing E. faecalis from the root canal used with three different irrigation methods. Materials and methods This study was conducted on freshly extracted maxillary incisors. After biomechanical preparation, root canals were injected with E. faecalis. Three groups were made which contained 30 teeth in each group; 2 mL of NaOCl solution was used for irrigation followed by agitation with K-files in group I; 2 mL of NaOCl solution was used for irrigation and ultrasonic agitation was done in group II. In group III, an alternate irrigation with NaOCl and 3% hydrogen peroxide was done. The fourth group (control) was irrigated with sterile saline solution. E. faecalis bacteria were sampled to the root canals with paper points and were transferred to tubes that contained 5 mL of brain heart infusion broth. Tubes were incubated and the presence of broth turbidity was suggestive of bacteria remaining in the root canal. Results All three groups showed no statistically significant difference. However, difference existed between experimental groups and control groups. Conclusion The author concluded that all three methods of application of NaOCl were effective in disinfecting the root canal than the saline solution. Clinical significance No single irrigant has 100% efficiency. Thus by this study, a best irrigating solution with maximum properties can be established. How to cite this article Priyank H, Pandey V, Bagul A, Majety KK, Verma P, Choudhury BK. Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study. J Contemp Dent Pract 2017;18(3):214-217.

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