scholarly journals Bacterial removal in flattened root canals with different tapers of ProDesign Logic single-file system

2021 ◽  
Vol 10 (9) ◽  
pp. e5910917701
Author(s):  
Vanessa Sandini ◽  
Viviane Godoy ◽  
Marina Carvalho Prado ◽  
Ricardo Ferreira ◽  
Adriana De-Jesus-Soares ◽  
...  
Keyword(s):  
Bacterial Biofilm ◽  
Bacterial Reduction ◽  
Sterile Saline ◽  
Root Canals ◽  
Bacterial Removal ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Level Of Significance ◽  
Post Hoc

Objective: This study evaluated the bacterial removal in flattened root canals after preparation with different tapers of ProDesign Logic instruments. Methodology: Fifty-five mandibular incisors with flattened root canals were contaminated with a pure culture of Enterococcus faecalis ATCC 29212 for 21 days at 37ºC. Root canals preparation was performed using ProDesign Logic and 0.9% sterile saline solution for irrigation. The specimens were randomly allocated to three experimental groups (n = 15) according to the taper of the instrument used: 25.03, 25.04, or 25.06. Intracanal bacteriological samples were taken before (S1) and after (S2) preparation and the number of colony-forming units per milliliter (CFU/mL) was calculated. Differences in bacterial counts before and after treatment, within each group, were analyzed by the paired t-test. Groups were compared by ANOVA with a post hoc test. The level of significance was set at p <0.05. Results: Mature biofilm formation was confirmed at root canal walls by scanning electron microscopy. Bacterial colonies were significantly reduced in all experimental groups at S2 compared to S1 (p <0.001). No significant difference was observed between groups regarding the percentage of bacterial reduction (p >0.05). Conclusion: Preparation of flattened root canals using ProDesign Logic instruments with tapers of 0.03, 0.04 and 0.06 resulted in similar planktonic and bacterial biofilm reduction. None of the instruments rendered root canals completely free of bacteria.

scholarly journals Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

2018 ◽  
Vol 29 (5) ◽  
pp. 459-464 ◽  
Author(s):  
Larissa Tais Soligo ◽  
Ediléia Lodi ◽  
Ana Paula Farina ◽  
Matheus Albino Souza ◽  
Cristina de Mattos Pimenta Vidal ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Enterococcus Faecalis ◽  
Grape Seed ◽  
Control Group ◽  
Bacterial Reduction ◽  
Calcium Hypochlorite ◽  
Root Canals ◽  
Colony Forming Units ◽  
Before And After ◽  
Post Hoc

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

scholarly journals Evaluation of the WaveOne Gold and One Shape New Generation in Reducing Enterococcus faecalis from Root Canal

2018 ◽  
Vol 29 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Raquel Esmeralda Guillén ◽  
Cleber Keiti Nabeshima ◽  
Hector Caballero-Flores ◽  
Miguel Roig Cayón ◽  
Montse Mercadé ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
File Systems ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Single File ◽  
Bacterial Removal ◽  
Significant Difference ◽  
Post Hoc ◽  
New Generation

Abstract The aim of this study was to evaluate the WaveOne Gold and One Shape New Generation systems regarding the bacterial removal from root canals infected with Enterococcus faecalis by comparing them to the conventional WaveOne and One Shape systems. Forty-eight distobuccal root canals of maxillary molars sterilized with ethylene oxide were infected with E. faecalis for 21 days, and then root canal initial bacterial sample was collected with paper cones and plated on M-enterococcus agar. The specimens were randomly divided into 4 groups according to the instrumentation: WaveOne Gold, One Shape New Generation, WaveOne and One Shape. After instrumentation, samples were collected with use of scraping and paper cones at immediate and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by Friedman test and intergroup analysis by Kruskal-Wallis with Dunn’s post-hoc test, all at 5% significance. All techniques significantly reduced the number of bacteria in the root canal (p<0.05). WaveOne Gold and One Shape New Generation promoted higher bacterial reduction than WaveOne and One Shape systems (p<0.05), but no significant difference was found between WaveOne Gold and One Shape New Generation or between WaveOne and One Shape (p>0.05). Novel single-file systems promote better bacterial removal than the conventional single-file systems.

scholarly journals Application of XP-Endo finisher files in the reduction of microbial load in oval-shaped root canals - An ex vivo study

2019 ◽  
Author(s):  
Giovanna Dornelas Mantovani ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Lidiane Mendes Louzada ◽  
Rodrigo Arruda Vasconcelos ◽  
Maria Cristina Carvalho ◽  
...  
Keyword(s):  
Saline Solution ◽  
Ex Vivo ◽  
Microbial Reduction ◽  
Bacterial Reduction ◽  
Significance Level ◽  
Root Canals ◽  
Colony Forming Units ◽  
Post Hoc ◽  
Cleaning Efficacy ◽  
Ex Vivo Study

The present study evaluated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in the reduction of Enterococcus faecalis in oval-shaped root canals. Eighty mandibular incisors with single oval-shaped root canals were contaminated with E. faecalis for 10 days at 37°C with centrifugation on alternate days. The teeth were randomly assigned to eight experimental groups (n=10) as follows: G1 – XP-Endo Shaper, G2 – XP-Endo Shaper + XP-Endo Finisher, G3 – Reciproc Blue, and G4 – Reciproc Blue + XP-Endo Finisher. Chemomechanical preparation was performed with saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Microbial reduction was assessed by colony forming units (CFU/mL) count before (S1) and after (S2) CMP by using sterile paper points. ANOVA, Tukey’s test and Bonferroni’s post-hoc tests were used at 5% significance level. Bacteria were present in all initial samples (P>.05). All instrumentation techniques reduced bacteria, irrespective of the irrigating solution (P<.05). With the use of NaCl, Reciproc Blue was more effective than XP-Endo Shaper (P=.035). The association of XP-Endo Finisher improved the cleaning efficacy of both systems (P=.239). With the use of NaOCl, XP-Endo Shaper and Reciproc Blue presented similar effectiveness (P=.779). XP-Endo Finisher enhanced the bacterial reduction of both systems tested (P<.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (P<.05).

The Effectiveness of Three Instrumentation Techniques on the Elimination of Entererococcus Faecalis from a Root Canal: An In Vitro Study

2005 ◽  
Vol 6 (1) ◽  
pp. 94-106 ◽  
Author(s):  
Meltem Çolak ◽  
Sinan Evcil ◽  
Y. Ziya Bayindir ◽  
Nimet Yigit
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Bacterial Cells ◽  
Sterile Saline ◽  
Root Canals ◽  
Rotary Instruments ◽  
Colony Forming Units ◽  
Before And After

Abstract The in vitro reduction of a bacterial population in a root canal by mechanical instrumentation using three techniques was evaluated. Root canals inoculated with a Enterococcus faecalis (E. faecalis) suspension were instrumented using hand Hedstroem files, Giromatic files, and Hero 642 rotary instruments. Irrigation was performed using sterile saline solution. Root canals were sampled before and after instrumentation. After serial dilutions, samples were plated onto Mitis-Salivarius agar and the colony forming units grown were counted. All instruments tested were able to significantly reduce the number of bacterial cells in the root canal, however, the results of this study indicated that Hedstroem files, Giromatic, and Hero 642 techniques were not significantly different in their ability to reduce intracanal bacteria. Citation Colak M, Evcil S, Bayindir Y, Yigit N. The Effectiveness of Three Instrumentation Techniques on the Elimination of Enterococcus Faecalis from a Root Canal: An In Vitro Study. J Contemp Dent Pract 2005 February;(6)1:094-106.

scholarly journals Evaluation of the Sealing Capability of the Internal Conical Connections of Implants with Titanium and Zirconia Abutments

2017 ◽  
Vol 18 (10) ◽  
pp. 915-922 ◽  
Author(s):  
Ilser Turkyilmaz ◽  
Damian L Black ◽  
Chol H Chong
Keyword(s):  
P Value ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Implant Abutment ◽  
Level Of Significance ◽  
Torque Values ◽  
Post Hoc ◽  
Positive Controls ◽  
Abutment Screw

ABSTRACT Aim The purpose of this in vitro investigation was to evaluate the sealing capability of the conical implant–abutment interfaces under different abutment screw torque values using titanium and zirconia abutments with Morse taper designs. Materials and methods A total of 42 dental implants (n = 21 for titanium abutments and n = 21 for zirconia abutments) were inoculated internally with three bacteria. These assemblies were divided into four test groups (n = 10) based on screw fixation torques of 35 or 20 Ncm and placed in sterile broth; the remaining abutments were used as positive controls and torqued to 10 Ncm. Microleakage was quantified by enumerating the bacteria from the colony-forming units. An analysis of variance for the estimates of bacteria enumerated and microgaps was used with a post hoc analysis as indicated. A p-value of 0.05 was used as the level of significance. Results There was no statistically significant difference in microleakage among the four test groups; there were no significant effects of screw torque or abutment type on the bacteria enumerated. There was a significantly smaller mean microgap with the zirconia abutments. Conclusion The results of this study indicated no statistically significant difference in the sealing capabilities between titanium and zirconia abutments, having internal conical connections, after increasing the abutment screw torque. Clinical significance It is important for clinicians to follow the guidelines suggested by the implant companies to avoid biomechanical complications over time. How to cite this article Black DL, Turkyilmaz I, Lien W, Chong CH. Evaluation of the Sealing Capability of the Internal Conical Connections of Implants with Titanium and Zirconia Abutments. J Contemp Dent Pract 2017;18(10):915-922.

Influence of moderate cigarette-smoking on the peri-implant clinicoradiographic inflammatory parameters around cement- and screw-retained dental implants

2020 ◽  
Author(s):  
Mana Alqahtani
Keyword(s):  
Cigarette Smoking ◽  
Dental Implants ◽  
Smoking History ◽  
Inflammatory Parameters ◽  
Significant Difference ◽  
Implant Retention ◽  
Level Of Significance ◽  
Soft Tissue Inflammation ◽  
Post Hoc ◽  
Adjustment Test

The aim was to assess the influence of moderate cigarette-smoking on the clinical (bleeding on probing [BoP] and probing depth [PD]) and radiographic (crestal bone resorption [CBR]) around cement- and screw-retained dental implants at 5 years’ follow-up. A questionnaire was used to collect information about age, gender, smoking history, duration of implants in function, jaw location of the implant, and daily toothbrushing and flossing. Peri-implant BoP, PD and CBR were measured in all groups. Group comparisons were performed using one-way analysis of variance and for multiple comparisons, the Bonferroni Post hoc adjustment test was performed. Level of significance was set at P&lt;0.05. Forty-eight patients (25 smokers and 23 non-smokers) had cement-retained dental implants; and 48 (24 smokers and 24 non-smokers) had screw-retained dental implants. Among patients with cement and screw-retained dental implants, PD (P&lt;0.05) and CBR (P&lt;0.05) were significantly higher among smokers than non-smokers. The peri-implant sites that demonstrated BoP were statistically significantly higher among non-smokers (P&lt;0.05) than smokers among patients with cement- and screw-retained dental implants. There was no statistically significant difference in peri-implant PD and CBR among smokers with cement- and screw-retained dental implants. Among non-smokers with cement and screw-retained dental implants, there was no statistically significant difference in BoP, PD and CBR. Cigarette-smoking is associated with an increased PD and CBR around cement- and screw-retained dental implants. Cigarette-smoking increases peri-implant soft tissue inflammation as well as loss of crestal bone and this relationship is independent of the type of implant retention protocol used.The author recommends that cement- and screw-retained dental implants are suitable for prosthesis restoration in non-smokers. Further studies on dual-smokers (individuals smoking cigarettes and other forms of tobacco products) are needed related to the clinicoradiographic inflammatory parameters around cement- and screw-retained dental implants

scholarly journals Biomechanical properties of the bone during implant placement

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ádám László Nagy ◽  
Zsolt Tóth ◽  
Tamás Tarjányi ◽  
Nándor Tamás Práger ◽  
Zoltán Lajos Baráth
Keyword(s):  
Bone Structure ◽  
Biomechanical Properties ◽  
Control Group ◽  
Dynamic Tests ◽  
Bone Model ◽  
Implant Placement ◽  
Significant Difference ◽  
Before And After ◽  
Group 2 ◽  
Post Hoc

Abstract Background In this research the biomechanical properties of a bone model was examined. Porcine ribs are used as experimental model. The objective of this research was to investigate and compare the biomechanical properties of the bone model before and after implant placement. Methods The bone samples were divided in three groups, Group 1 where ALL-ON-FOUR protocol was used during pre-drilling and placing the implants, Group 2 where ALL-ON-FOUR protocol was used during pre-drilling, and implants were not placed, and Group 3 consisting of intact bones served as a control group. Static and dynamic loading was applied for examining the model samples. Kruskal–Wallis statistical test and as a post-hoc test Mann–Whitney U test was performed to analyze experimental results. Results According to the results of the static loading, there was no significant difference between the implanted and original ribs, however, the toughness values of the bones decreased largely on account of predrilling the bones. The analysis of dynamic fatigue measurements by Kruskal–Wallis test showed significant differences between the intact and predrilled bones. Conclusion The pre-drilled bone was much weaker in both static and dynamic tests than the natural or implanted specimens. According to the results of the dynamic tests and after a certain loading cycle the implanted samples behaved the same way as the control samples, which suggests that implantation have stabilized the skeletal bone structure.

scholarly journals Evaluation of color stability of different temporary restorative materials

2015 ◽  
Vol 44 (5) ◽  
pp. 262-267 ◽  
Author(s):  
José Vitor Quinelli Mazaro ◽  
Luiz Miguel Minani ◽  
Adriana Cristina Zavanelli ◽  
Caroline Cantieri de Mello ◽  
Cleidiel Aparecido Araújo Lemos
Keyword(s):  
Storage Time ◽  
Color Change ◽  
Artificial Saliva ◽  
Color Stability ◽  
Time Interval ◽  
Acrylic Resins ◽  
Significant Difference ◽  
Restorative Materials ◽  
Before And After ◽  
Level Of Significance

AbstractIntroductionTemporary restorative materials are widely used, however, little is know about their color stability.Objectiveto evaluate the color stability of the following temporary restorative materials: acrylic and bis-acrylic resins after immersion in pigmenting solutions for different periods of storage.Material and methodFour materials were tested (Dêncor/Clássico, Protemp 4/3M ESPE; Structur 2 SC/Voco; Luxatemp AM Plus/DMG) and 30 test specimens (15 mm in diameter and 2 mm thick) per material were fabricated. They were divided according to the storage medium (artificial saliva, saliva + cola type soda, and saliva + coffee) and storage time intervals (2, 5, 7 and 15 days). Color measurements were made before and after immersions, with use of a spectrophotometer, by means of the CIE L*a*b* system. The data were analyzed by the analysis of variance and the Tukey Test, at a level of significance of 5%.ResultAcrylic resin presented greater color stability in comparison with bis-acrylic resins (p<0.001). When bis-acrylic resins were compared no significant difference was observed between the resins Structur and Luxatemp (p=0.767). As regards solutions tested, coffee showed the highest color change values (p<0.001), and the longer the storage time interval, the greater was the color change in all the temporary restorative materials analyzed (p<0.001).ConclusionAcrylic resin presented greater color stability in comparison with bis-acrylic resins (p<0.001). Coffee caused the greatest color change, and immersion time was determinant in color stability of the temporary materials analyzed.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals Fracture resistance of immature teeth filled with mineral trioxide aggregate, bioaggregate, and biodentine

2016 ◽  
Vol 10 (02) ◽  
pp. 220-224 ◽  
Author(s):  
Emre Bayram ◽  
Huda Melike Bayram
Keyword(s):  
Fracture Resistance ◽  
Mineral Trioxide Aggregate ◽  
Mean Values ◽  
Root Canals ◽  
Immature Teeth ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Post Hoc ◽  
Group 1

ABSTRACT Objective: The purpose of this study was to evaluate fracture resistance of teeth with immature apices treated with coronal placement of mineral trioxide aggregate (MTA), bioaggregate (BA), and Biodentine. Materials and Methods: Forty-one freshly extracted, single-rooted human premolar teeth were used for the study. At first, the root length was standardized to 9 mm. The crown-down technique was used for the preparation of the root canals using the rotary ProTaper system (Dentsply Maillefer, Ballaigues, Switzerland) of F3 (30). Peeso reamer no. 6 was stepped out from the apex to simulate an incompletely formed root. The prepared roots were randomly assigned to one control (n = 5) and three experimental (n = 12) groups, as described below. Group 1: White MTA (Angelus, Londrina, Brazil) was prepared as per the manufacturer's instructions and compacted into the root canal using MAP system (Dentsply Maillefer, Ballaigues, Switzerland) and condensed by pluggers (Angelus, Londrina, Brazil). Group 2: The canals were filled with DiaRoot-BA (DiaDent Group International, Canada). Group 3: Biodentine (Septodont, Saint Maur des Fosses, France) solution was mixed with the capsule powder and condensed using pluggers. Instron was used to determine the maximum horizontal load to fracture the tooth, placing the tip 3 mm incisal to the cementoenamel junction. Mean values of the fracture strength were compared by ANOVA followed by a post hoc test. P < 0.05 was considered statistically significant. Results: No significant difference was observed among the MTA, BA, and biodentine experimental groups. Conclusion: All the three materials tested, may be used as effective strengthening agents for immature teeth.

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