scholarly journals Rangeliosis: histopathological analysis, hematology and molecular detection of canine Rangelia vitalii in Rio Grande do Sul, Brazil

2017 ◽  
Vol 47 (10) ◽  
Author(s):  
Gabriela Fredo ◽  
Ronaldo Viana Leite-Filho ◽  
Camila De Ávila Pietzsch ◽  
Caroline Pinto de Andrade ◽  
Naila Cristina Blatt Duda ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Plasma Cells ◽  
Infectious Agent ◽  
Rio Grande ◽  
Extramedullary Hematopoiesis ◽  
Lymphoid Hyperplasia ◽  
Histopathological Analysis ◽  
Rio Grande Do Sul ◽  
Genetic Sequencing

ABSTRACT: In the period from January 2004 to December 2015, 56 dogs were diagnosed with rangeliosis in the Setor de Patologia Veterinária at Universidade Federal do Rio Grande do Sul (SPV-UFRGS). The main hematological abnormalities were thrombocytopenia and anemia. The affected dogs showed signs of apathy, anorexia, fetid and bloody diarrhea, vomiting, and dehydration. At necropsy, the main changes were jaundice, splenomegaly, hepatomegaly, and lymphadenomegaly. Histological analyses revealed parasitophorous vacuoles of Rangelia vitalii in cytoplasmic endothelial cells, mainly in the heart, kidneys, lymph nodes, intestines, and pancreas. Inflammation characterized by mononuclear cells was predominant in the analysis, and most was due to the presence of plasma cells. Other lesion types observed were lymphoid hyperplasia, extramedullary hematopoiesis, erythrophagocytosis, and erythroid lineage hyperplasia in bone marrow. Of the total number of animals, 49 were diagnosed using necropsy and histological analysis, and seven were diagnosed using a molecular analysis (i.e., PCR and genetic sequencing of blood samples). This paper presented a different method of diagnosing rangeliosis in canines. This approach involved histological methods including the quantification and determination of the intensity and distribution of the infectious agent in different organs.

scholarly journals CHEMICAL CHARACTERIZATION OF ATMOSPHERIC PARTICLES IN URBAN AREAS OF RIO GRANDE DO SUL, BRAZIL-PRELIMINARY STUDY

2006 ◽  
Vol 14 (14) ◽  
pp. 1-16
Author(s):  
E. Teixeira ◽  
J. Fachel Braga ◽  
J.D. D. Migliavacca ◽  
M.L.L.Fomoso Sanchez
Keyword(s):  
Chemical Composition ◽  
Urban Areas ◽  
Chemical Characterization ◽  
Rio Grande ◽  
Atmospheric Particles ◽  
Rio Grande Do Sul ◽  
Power Stations ◽  
Cluster Data ◽  
Steel Industries

This work reports the determination of the concentration and chemical composition of atmospheric particles in the urban districts of Charqueadas and Sapucaia do Sul, State of Rio Grande do Sul, Brazil. Chemical composition, morphology, and particle size were analyzed using a scanning electron microscope with energy dispersive x=ray microanalysis (SEM-EDS). Cluster analysis showed that there were six types of particles: Fe-Zn, Fe, Si=Al, Si, Ca-S, and Na. Factorial analysis from cluster data showed that particles rich in Fe-Zn, Si-Al, and Ca-S appeared more frequently, indicating anthropogenic influence (vehicles, steel plants, coal-fired power stations). The experimental results and consideration of the wind directions show that the main source of pollution in Charqueadas appears to be due to coal mining and steel industries, while in Sapucaia do Sul due to steel plants and vehicles.

scholarly journals Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 741 ◽  
Author(s):  
Ying Zou ◽  
Guosi Xie ◽  
Tianchang Jia ◽  
Tingting Xu ◽  
Chong Wang ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Infectious Agent ◽  
Systematic Investigation ◽  
Shrimp Farming ◽  
Histopathological Analysis ◽  
Penaeus Vannamei ◽  
Challenge Tests ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Farming Industry

A new emerging disease called “translucent post-larvae disease” (TPD) or “glass post-larvae disease” (GPD) of Penaeus vannamei, characterized by pale or colorless hepatopancreas and digestive tract, has become an urgent threat to the shrimp farming industry. Following this clue that treatment of an antibacterial agent could alleviate the disease, systematic investigation of the potential infectious agent of TPD was conducted using bacterial identification and artificial challenge tests to fulfill Koch’s postulates. A dominant bacterial isolate, Vp-JS20200428004-2, from the moribund individuals was isolated and identified as Vibrio parahaemolyticus based on multi-locus sequence analysis. However, Vp-JS20200428004-2 differed from the V. parahaemolyticus that caused typical acute hepatopancreatic necrosis disease. Immersion challenge tests revealed that Vp-JS20200428004-2 could cause 100% mortality within 40 h at a dose of 1.83 × 106 CFU/mL, and experimental infected shrimp showed similar clinical signs of TPD. The Vp-JS20200428004-2 could be re-isolated and identified from the experimental infected individuals. Moreover, histopathological analysis of diseased samples indicated that Vp-JS20200428004-2 caused severe necrosis and sloughing of epithelial cells of the hepatopancreas and midgut in shrimp individuals both naturally and experimentally infected. Our present results indicated that Vp-JS20200428004-2 is a highly virulent infectious agent associated with the TPD and deserves further attention.

Determination of cocaine and its main adulterants in seized drugs from Rio Grande do Sul, Brazil, by a Doehlert optimized LC-DAD method

2016 ◽  
Vol 8 (26) ◽  
pp. 5212-5217 ◽  
Author(s):  
M. C. A. Marcelo ◽  
T. R. Fiorentin ◽  
K. C. Mariotti ◽  
R. S. Ortiz ◽  
R. P. Limberger ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Rio Grande ◽  
The State ◽  
Array Detector ◽  
Diode Array ◽  
Rio Grande Do Sul ◽  
Diode Array Detector

A liquid chromatography with a diode array detector (LC-DAD) method was developed through Doehlert optimization for analysis of cocaine seized in the state of Rio Grande do Sul, Brazil.

scholarly journals Evaluation of sperm quality of Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae)

2017 ◽  
Vol 77 (3) ◽  
pp. 553-557
Author(s):  
A. C. Silva ◽  
A. S. Varela Junior ◽  
T. F. Cardoso ◽  
E. F. Silva ◽  
D. Loebmann ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Coastal Region ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Sperm Analysis ◽  
Pampa Region

Abstract Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.

scholarly journals Potabilidade da água de poços artesianos: diagnóstico de amostras dos municípios do Rio Grande do Sul

2019 ◽  
Vol 41 ◽  
pp. 1
Author(s):  
Sandra Cadore Peixoto ◽  
Carolina Denardi Merlugo ◽  
Anderson Maciel ◽  
Tiago Barboza Solner
Keyword(s):  
Water Quality ◽  
Ph Value ◽  
Iron Concentration ◽  
Rio Grande ◽  
Natural State ◽  
Human Consumption ◽  
Rio Grande Do Sul ◽  
Artesian Wells ◽  
Micro Organisms

Drinking water is all water for consumption. It must have certain amount of dissolved minerals, be free of toxic materials and micro-organisms. It is considered an indispensable resource for man. One of the main problems for the use of water is related to its contamination, that is, this resource has been polluted in such a way that it can no longer consume it in its natural state. However, despite all the efforts to store and reduce its consumption, it is becoming scarce and its quality deteriorates faster and faster. In this way, groundwater has become an important alternative for water supply to communities. Considering the relevance of this theme, this study seeks to make a diagnosis of the water quality of artesian wells of the Central Region of the State of Rio Grande do Sul, in order to prove the potability. For these determinations the experimental procedures were: determination of the pH value; turbidity; temperature; total solids dissolved; color; hardness and residual iron concentration. The evaluation of the results obtained in this study was based on the standards of potability established in Ordinance No. 2,914, of December 12, 2011, of the Ministry of Health, which establishes the procedures for control and surveillance of water quality for human consumption and its standard of potability.Drinking water is all water for consumption. It must have certain amount of dissolved minerals, be free of toxic materials and micro-organisms. It is considered an indispensable resource for man. One of the main problems for the use of water is related to its contamination, that is, this resource has been polluted in such a way that it can no longer consume it in its natural state. However, despite all the efforts to store and reduce its consumption, it is becoming scarce and its quality deteriorates faster and faster. In this way, groundwater has become an important alternative for water supply to communities. Considering the relevance of this theme, this study seeks to make a diagnosis of the water quality of artesian wells of the Central Region of the State of Rio Grande do Sul, in order to prove the potability. For these determinations the experimental procedures were: determination of the pH value; turbidity; temperature; total solids dissolved; color; hardness and residual iron concentration. The evaluation of the results obtained in this study was based on the standards of potability established in Ordinance No. 2,914, of December 12, 2011, of the Ministry of Health, which establishes the procedures for control and surveillance of water quality for human consumption and its standard of potability.

scholarly journals Comparação entre dois modelos de função footprint aplicados ao sítio experimental de Pedras Altas

2018 ◽  
Vol 40 ◽  
pp. 199
Author(s):  
Tiago Bremm ◽  
Ivan Mauricio Cely Toro ◽  
Gustavo Pujol Veeck ◽  
Débora Regina Roberti ◽  
Lidiane Buligon
Keyword(s):  
Stochastic Model ◽  
Analytical Model ◽  
High Frequency ◽  
Rio Grande ◽  
Field Of View ◽  
Western Region ◽  
Rio Grande Do Sul ◽  
Surface Flows ◽  
Atmospheric Data

Micrometeorological towers are installed in several regions in order to collect atmospheric data at high frequency for the estimation of micrometeorological parameters and surface flows of energy and mass. The determination of the towers field of view and, therefore, the flow measured at the sensors is defined by the footprint, which is directly influenced by the geometry of the terrain and by the vegetation of the site on which the sensor is installed. In this way, the present work aims to analyze the predominant area of the flow contribution by two different methods of footprint determination: one analytical model and one lagrangian stochastic. The data were analyzed for the micrometeorological station of Pedras Altas, located in the south western region of Rio Grande do Sul. The results show that the stochastic model considers the tower field of view closer than the analytical model, consequently it covers a smaller area.

scholarly journals Embryonic external morphogenesis of Rhammatocerus conspersus (Bruner) (Orthoptera: Acrididae: Gomphocerinae) and determination of the diapausing embryonic stage

1998 ◽  
Vol 27 (4) ◽  
pp. 557-583 ◽  
Author(s):  
Cintia C. Niva ◽  
Miriam Becker
Keyword(s):  
Embryonic Development ◽  
Rio Grande ◽  
Embryonic Stage ◽  
Embryonic Diapause ◽  
Rio Grande Do Sul

The grasshopper Rhammatocerus conspersus (Bruner) (Orthoptera: Acrididae: Gomphocerinae) is an occasional pest in pasturelands of Rio Grande do Sul State. It is a univoltine species with an embryonic diapause. Nymphal and adult stages occur during the warmer months (November-March). Eggs were dissected periodically for characterization of embryonic external morphogenesis in 1994 and 1995. Ten embryonic stages were illustrated. Two diapausing stages were verified in R. conspersus: one at 25% and another at 50% of total embryonic development.

Determination of Maximum Water Infiltration Areas in Vacacaí-Mirim Basin in Southern Brazil

2021 ◽  
Vol 44 ◽  
Author(s):  
Pedro Daniel Da Cunha Kemerich ◽  
Willian Fernando De Borba ◽  
Masato Kobiyama ◽  
Sérgio Roberto Martins ◽  
Galileo Adeli Buriol ◽  
...  
Keyword(s):  
Rio Grande ◽  
Water Infiltration ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Maximum Water ◽  
Santa Maria

A determinação das taxas de infiltração de água no solo é de fundamental importância, pois além de determinar a recarga de aquíferos estima a vulnerabilidade a contaminação a qual o mesmo está sujeito. Com base nisso o presente trabalho tem por objetivo determinar o índice de infiltração de água no solo pelo método DRASTIC na bacia hidrográfica do rio Vacacaí-Mirim, no estado do Rio Grande do Sul. Para a determinação desses índices, seguiu-se a metodologia descrita por Oliveira e Ferreira (2002), onde as análises para os parâmetros físicos foram obtidos no laboratório de física do solo da Universidade Federal de Santa Maria, campus de Frederico Westphalen-RS. Os índices de facilidade a infiltração nos anos de 2010 e 2011 variaram de 10 (áreas pouco fissuradas) a 30 (áreas com altas perfurações). Com relação as áreas de infiltração máxima, no ano de 2010, 24 % da área da bacia apresentou tal condição, já para o ano de 2011, esse percentual caiu para 18,55 %. Com base nisso, a metodologia se mostra interessante para a determinação das taxas de infiltração de água na bacia hidrográfica do rio Vacacaí-Mirim. As áreas que mais se destacaram com as taxas de infiltração máxima localizam-se nas várzeas da bacia hidrográfica, onde predominam o cultivo de arroz, sendo assim é de fundamental importância a preservação de tais áreas com vista a manutenção da qualidade da água subterrânea no local.

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