scholarly journals Tobacco waste hydrolysate of stem and root of the tobacco plant for biostimulation in rice and corn seed germination

2020 ◽  
Vol 50 (8) ◽  
Author(s):  
Paulo Roberto Fetter ◽  
Manuela Gassen ◽  
Lucélia Hoehne ◽  
Michele Hoeltz ◽  
Lisianne Brittes Benitez ◽  
...  

ABSTRACT: Stimulation of seed germination may be due to acceleration of germination as well as due to seedling growth-promotion during early development. Plant hydrolysate can be applied as a stimulant. Thus, we aimed to verify the influence of the hydrolysates, obtained by alkaline or acid treatment, from tobacco (Nicotiana tabacum L.) crop residues (root and stem) on the seed germination process. Seed germination was studied with Oryza sativa (rice) and Zea mays (corn). Sixteen germination experiments of 50 seeds each were undertaken, with 4 replicates, soaked with hydrolysates diluted at 20 and 80%, in 2 and 3 mL of hydration volumes for 48 h. Germinated seeds were counted, at which point radicular protrusions were observed. Rootlets and aerial parts were collected, dried and weighed. The hydrolysates presented seedling nutrition potential to the corn, with ~50% more mass compared to the results with water at the same conditions, and the germination acceleration was not significant. For the tested rice seeds, the results were reversed, and the germination acceleration was significant with rates up to 94% after 48 h of incubation. Better results of germination were obtained with hydrolysate from acid treatment, and root or stem tobacco can be used for this purpose.

1990 ◽  
Vol 82 (3) ◽  
pp. 555-560 ◽  
Author(s):  
V. L. Martin ◽  
E. L. McCoy ◽  
W. A. Dick

Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.


2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.


Rodriguésia ◽  
2016 ◽  
Vol 67 (3) ◽  
pp. 661-666
Author(s):  
João Fabrício Mota Rodrigues ◽  
Sâmia Paiva de Oliveira

Abstract Ingestion of seeds by vertebrates may influence seed germination and dispersion. However the mechanisms responsible by these effects and the importance of turtles in these interactions are still poorly understood. This study aims to evaluate how Chelonoidis carbonarius influences the germination process of Leucaena leucocephala using an experiment conducted at the Federal University of Ceará, Fortaleza-CE, Brazil, that simulates the passage of the seeds of this plant through the digestive tract of C. carbonarius: stomach acidic condition and seeds sown in feces. We demonstrated that sowing the seeds in feces of Chelonoidis carbonarius has a negative effect on the germination of Leucaena leucocephala, decreasing the germination speed index of the seeds, while the acidic conditions have a neutral one. This study is a first attempt to understand how turtles and tortoises may influence the seed germination, and the implications of these findings in natural environments deserve further investigations.


AGRICA ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 1-8
Author(s):  
Maria Goreti Firma

This study aims to determine the effect of tobacco plant extracts on mortality of armyworm pests (Spodoptera litura F.) and determine the optimum concentration of tobacco leaf extracts on mortality of armyworm caterpillars on mustard plants in the field. The design used in this study was a Randomized Block Design (RBD) consisting of 5 treatments and 4 replications namely T1: 175 ml/L concentration of tobacco leaf extract, T2: 350 ml/L concentration of tobacco leaf extract T3: Concentration of 525 ml/L tobacco leaf extract, T4: Concentration of 700 ml/L tobacco leaf extract, T5: Concentration of 875 ml/L of tobacco leaf extract. The observation variables in this study were pest mortality (%), damage intensity (%), plant fresh weight, and fresh weight per hectare (ton). The results showed that the concentration of tobacco leaf extracts significantly affected the mortality of Spodoptera litura F. At the concentration of T5 treatment (875 ml) were the lowest damage, pest mortality, fresh weight of the mustard plant, and fresh weight per hectare of was 9.75%, 88%, 109.25 gr, and27.31 tons respectively.


2020 ◽  
Vol 42 ◽  
Author(s):  
Michel Esper Neto ◽  
David W. Britt ◽  
Kyle Alan Jackson ◽  
Alessandro Lucca Braccini ◽  
Tadeu Takeyoshi Inoue ◽  
...  

Abstract: Fertilizer formulation alternatives that avoid unnecessary losses and environmental impacts are being investigated in agricultural management. Seed priming with nanofertilizers prior to planting, reduces concerns about non-target dispersion; however, priming formulations and concentrations must be carefully selected to avoid undesired effects. Here, seed germination and seedling development were evaluated after seed priming with CuO nanoparticles (NPs), CuO bulk and CuCl2. The seeds were immersed in priming solutions of 0, 20, 40, 80 and 160 mg.L−1 Cu for the three Cu sources. Following 8 hours priming, the seeds were evaluated for germination and vigor (first germination count). Root and shoot lengths were measured as well as shoot and root dry biomass. The copper NP did not show any toxic effects on corn seed germination and growth, and also promoted higher biomass when compared to the other Cu sources. On the other hand, CuCl2 primed seeds exhibited Cu-toxicity in roots and shoots for all concentrations tested. Bulk Cu priming results indicated the better role of NPs size effects. These findings support NP-seed priming as an alternative to delivery of essential micronutrients, such as copper, to corn seedlings.


2021 ◽  
Vol 43 ◽  
Author(s):  
Marcelo Coelho Sekita ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Daniel Teixeira Pinheiro ◽  
Aparecida Leonir da Silva ◽  
Antônio César Batista Matos ◽  
...  

Abstract: Nitric oxide (NO) can act in biochemical pathways of the germination process; however, there is little information about how it acts on the performance of pea seeds. The aim of this study was to evaluate the physiological and biochemical effects of NO on pea seed germination and vigor. Pea seeds cv. Itapuã 600 obtained from three seed lots with different levels of physiological quality were sown in a substrate moistened with water (control) or sodium nitroprusside (SNP) solution, a NO donor (50 μM), to assess germination, vigor, activity of antioxidant enzymes, reactive oxygen species, lipid peroxidation, and amylase activity. NO application does not alter pea seed germination, but it increases vigor. It is more effective in seeds with lower physiological potential. In addition, NO leads to reduction in oxidative stress, favors the translocation of reserves to the embryo, and has potential for use in the treatment of pea seeds to increase seed vigor.


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