scholarly journals Cytotoxicity of aqueous extracts of Rosmarinus officinalis L. (Labiatae) in plant test system

2014 ◽  
Vol 74 (4) ◽  
pp. 886-889 ◽  
Author(s):  
GHS Cardoso ◽  
EBS Dantas ◽  
FRC Sousa ◽  
AP Peron
Keyword(s):  
Cell Cycle ◽  
Test System ◽  
Rosmarinus Officinalis ◽  
Control Group ◽  
Leaf Extracts ◽  
Root Cells ◽  
Plant Test ◽  
Rosmarinus Officinalis L ◽  
Meristematic Root ◽  
Number Of Cells

This study investigated the cytotoxic activity of Rosmarinus officinalis L. (rosemary) aqueous extract on the cell cycle of Allium cepa. To this end, crude aqueous leaf extracts at four concentrations, 0.02, 0.04, 0.06 and 0.08 mg/mL, were tested on A. cepa meristematic root cells, at exposure times of 24 and 48h. Slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5,000 for each control group and concentration. The four concentrations tested, including the lowest and considered ideal for use, at all exposure times, showed a significant antiproliferative effect on the cell cycle of this test system and presented a high number of cells in prophase. Our results evidenced the cytotoxicity of rosemary extracts, under the studied conditions.

scholarly journals Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

2014 ◽  
Vol 86 (3) ◽  
pp. 1147-1150 ◽  
Author(s):  
LOURRAN P. LACERDA ◽  
GEIZ MALAQUIAS ◽  
ANA PAULA PERON
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Test System ◽  
Control Group ◽  
Aqueous Extracts ◽  
Root Cells ◽  
Antiproliferative Action ◽  
Allium Cepa L ◽  
Meristematic Root ◽  
Number Of Cells

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

scholarly journals Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L.

2014 ◽  
Vol 86 (3) ◽  
pp. 1131-1137 ◽  
Author(s):  
ERASMOVLANE S.B. NEVES ◽  
PAULO MICHEL PINHEIRO FERREIRA ◽  
LEONARDO H.G.M. LIMA ◽  
ANA PAULA PERON
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Root Meristem ◽  
Test System ◽  
Aqueous Extracts ◽  
Phyllanthus Niruri ◽  
Root Cells ◽  
Allium Cepa L ◽  
Chi Squared ◽  
Meristematic Root

This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical analysis (p<0.05). From the results obtained it was observed that all four concentrations tested had significant antiproliferative effect on the cell cycle of this test system. We also found the presence of cellular aberrations such as colchicined metaphases, anaphasic and telophasic bridges, and micronuclei in the two exposure times for all concentrations evaluated. Therefore, under the conditions studied the concentrations of aqueous extracts of leaves of P. niruri showed to be cytotoxic and genotoxic.

scholarly journals The Effect of Rosemary (Rosmarinus officinalis. L) Plant Extracts on the Immune Response and Lipid Profile in Mice

2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Fawzi Mohammad Al Sheyab ◽  
Nizar Abuharfeil ◽  
Lina Salloum ◽  
Rehan Bani Hani ◽  
Dalal Saleh Awad
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coconut Oil ◽  
Normal Diet ◽  
Rosmarinus Officinalis ◽  
Immunoglobulin M ◽  
Rosemary Extract ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Rosmarinus Officinalis L

The hypolipidemic activity of leaves of rosemary (Rosmarinus officinalis), a herbal plant was studied. Three groups of BALB/c mice were treated as following: the first group was fed with normal diet (sugar & water), the second group was fed with high cholesterol (HC) (2% by weight) and coconut oil (25% by weight), for 36 days, and the third group was fed with HC diet and given 100 mg/kg rosemary extract (10% w/v) during the last 15 days of treatment period. There was a significant decrease (P < 0.001) in plasma total cholesterol (TC)(- 68.57%), low density lipoprotein (LDL) (- 56.34%), and triglycerides (TG) (- 182.61%). A significant increase in high density lipoprotein (HDL) (38.53%) was obtained in rosemary-fed mice compared to HC mice. In another experiment, the immunomodulatory activity of aqueous extract of R. officinalis was evaluated in BALB/c mice. Mice were treated with three doses of extract (10, 50, 100 mg/kg body weight) for 8 weeks. Humoral immunity against membrane proteins of sheep erythrocytes measured by ELISA showed that IgM (Immunoglobulin M) response significantly increased by 26.95%, 36.5%, 70.78% respectively, in mice fed with 10, 50, and 100 mg/kg of rosemary as compared to the control group (P < 0.05). IgG (Immunoglobulin G) response increased significantly at all antibody titers, in mice fed with rosemary at doses of 10, 50, and 100 mg/kg. Meanwhile, there was no significant different in IgM and IgG responses between 50 and 100 mg/kg (P > 0.05). On the other hand, concanavalin A-stimulated proliferation of spleen cells from mice fed with 100 mg rosemary extract was significantly higher by 57% than that of cells from the corresponding control animals.

scholarly journals ANTIFUNGAL ACTIVITIES OF ETHANOL EXTRACT OF ROSEMARY LEAF (Rosemarinus officinalis L.) AGAINST Candida albicans

2020 ◽  
Vol 2 (1) ◽  
pp. 28
Author(s):  
Dewa Ayu Made Adnya Swari ◽  
I Wayan Martadi Santika ◽  
I Gusti Made Aman
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Rosmarinic Acid ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Control Group ◽  
Rosmarinus Officinalis L

Background: This study was a preliminary study aimed to investigating the in vitro activity of rosmarinic acid contained on Rosemary leaves (Rosemarinus officinalis L.) on the Candida albicans. Rosmarinic acid can be an inhibitor of the RTPase enzyme in the fungus Candida albicans. Objective: This study is expected to provide information in the development of science about the antifungal activity of ethanol extract of Rosemary leave (Rosmarinus officinalis L.) againsts C.albicans. The recent research was an laboratory experimental. Experiments on an ethanol extract of Rosemary leaves (Rosemarinus officinalis L.) and post-test design of the control group. Methods: The extraction method used in this study was a multilevel extraction method. The antifungal activity testing method uses discs and MIC methods. Replication was done six times at two concentration namely, 80% (b/v) and 100% for each measurement. Results: The data analysis was carried out using SPSS with 95% of confidence level with the Kruskal Wallis analysis then completed with the Mann Whitney U test. The results of the MIC experiment showed all the various concentrations of ethanol extract performed Candida albicans growth on the surface of the media. Extract with 80% (b/v) concentration showed greater antifungal activity. Furthermore the MIC was shown by 80% (b/v) concentration of the ethanol extract of Rosemary leaves against Candida albicans. The test results of the antifungal activity of the Rosemary leaf ethanol extract showed the presence of inhibitory diameter in the ethanol extract of the Rosemary leaf concentration of 80% (b/v) with a mean ± SD of 10.6 ± 0.57735 and RSD of 5.774%. Conclusion: The ethanol extract of Rosemary leaves (Rosmarinus officinalis L.) has an inhibitory effect on the fungus Candida albicans at a concentration of 80% (b / v) Keywords: Antifungal activity, Candida albicans, Rosemary, RTPase, ethanol extract

scholarly journals Rosmarinus officinalis L. (Rosemary): An Ancient Plant with Uses in Personal Healthcare and Cosmetics

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 77
Author(s):  
Francisco José González-Minero ◽  
Luis Bravo-Díaz ◽  
Antonio Ayala-Gómez
Keyword(s):  
Folk Medicine ◽  
Rosmarinus Officinalis ◽  
Carnosic Acid ◽  
Leaf Extracts ◽  
Ancient Egyptian ◽  
High Prevalence ◽  
Perennial Shrub ◽  
In The Wild ◽  
Rosmarinus Officinalis L ◽  
Derivatives Of

This work is a bibliographical review of rosemary (Rosmarinus officinalis) that focuses on the application of derivatives of this plant for cosmetic products, an application which has been recognized and valued since Ancient Egyptian times. Rosemary is a plant of Mediterranean origin that has been distributed throughout different areas of the world. It has many medicinal properties, and its extracts have been used (mainly orally) in folk medicine. It belongs to the Labiatae family, which contains several genera—such as Salvia, Lavandula, and Thymus—that are commonly used in cosmetics, due to their high prevalence of antioxidant molecules. Rosemary is a perennial shrub that grows in the wild or is cultivated. It has glandular hairs that emit fragrant volatile essential oils (mainly monoterpenes) in response to drought conditions in the Mediterranean climate. It also contains diterpenes such as carnosic acid and other polyphenolic molecules. Herein, the botanical and ecological characteristics of the plant are discussed, as well as the main bioactive compounds found in its volatile essential oil and in leaf extracts. Afterward, we review the applications of rosemary in cosmetics, considering its preservative power, the kinds of products in which it is used, and its toxicological safety, as well as its current uses or future applications in topical preparations, according to recent and ongoing studies.

scholarly journals Effect of Hydroalcoholic Extract of Rosmarinus officinalis L. Leaf on Anxiety in Mice

2016 ◽  
Vol 21 (4) ◽  
pp. NP85-NP90 ◽  
Author(s):  
Mosayeb Noori Ahmad Abadi ◽  
Mohsen Mortazavi ◽  
Navid Kalani ◽  
Hadi Zare Marzouni ◽  
Wesam Kooti ◽  
...  
Keyword(s):  
Side Effects ◽  
Statistical Tests ◽  
Rosmarinus Officinalis ◽  
Rosemary Extract ◽  
Control Group ◽  
Standard Drug ◽  
Hydroalcoholic Extract ◽  
Plus Maze ◽  
Significant Difference ◽  
Rosmarinus Officinalis L

In today’s stressful world, psychopathy (especially anxiety) is receiving increased importance. Most of the drugs used to treat this disease have several side effects. Medicinal plants derived from natural products have fewer side effects and can be used in the treatment of this disease. The aim of this study was to evaluate the effect of the hydroalcoholic extract of Rosmarinus officinalis L. on anxiety in mice. In this experimental study, 50 male mice were randomly divided into 5 groups. To evaluate anxiety, the Elevated Plus Maze test was performed. The control group received normal saline, the positive control group received diazepam (1 mg/kg) as intraperitoneal injection, and the experimental groups received doses of 100, 200, and 400 mg/kg body weight of rosemary extract. The data were analyzed using SPSS 15 and ANOVA statistical tests. The results show that rosemary extract dose-dependently increases the mice spending time and the entries number of mice in plus maze open arms (indicating less stress). This effect at a dose of 400 mg/kg was similar to diazepam, which, in comparison to the control group, was statistically significant ( P < .01), while the evaluation of locomotor activity in treated groups, compared with the control groups, showed no significant difference ( P > .05). On the other hand, the rosemary extract, similar to the standard drug diazepam, showed an anti-anxiety effect. This effect is probably due to the presence of flavonoids in this plant and their antioxidant property.

scholarly journals GC-MS Analysis of Methanolic Extract of Leaves of Rosmarinus officinalis L.

2020 ◽  
Vol 2 (1) ◽  
pp. 68
Keyword(s):  
Medicinal Plants ◽  
Crude Extract ◽  
Mediterranean Region ◽  
Methanolic Extract ◽  
Rosmarinus Officinalis ◽  
Natural Source ◽  
Leaf Extracts ◽  
Scientific Communities ◽  
Pharmaceutical Science ◽  
Rosmarinus Officinalis L

Currently, medicinal plants are gaining importance in pharmaceutical and scientific communities. Medicinal plants are the richest natural source of valuable phytochemicals, which can be very useful to treat human diseases and their dysfunctions. Rosmarinus officinalis L. is an important medicinal shrub that belongs to family Lamiaceae and is native to the Mediterranean region. During the present work, an investigation on the photochemical profiling of Rosmarinus officinalis leaves was done. The extraction was made by maceration using methanol as a solvent, and the dried crude extract was analyzed by GC-MS analyzer. Twenty-six compounds were observed from the leaf extracts and found that they have great significance in pharmaceutical science for therapeutically efficient formulations in order to combat various diseases.

Cytotoxic Effect of Rosmarinus officinalis L. Leaf Extracts on Tumor Cell Line

2015 ◽  
Vol 18 (4) ◽  
pp. 98-102 ◽  
Author(s):  
Shahlaa M. Salih ◽  
◽  
Khalid H. Alobaidi ◽  
Zinah F. Alobaidi ◽  
◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Tumor Cell Line ◽  
Rosmarinus Officinalis ◽  
Leaf Extracts ◽  
Rosmarinus Officinalis L

FORMULADO DE ALECRIM NO CONTROLE DE ANTRACNOSE E CONSERVAÇÃO EM PÓS-COLHEITA DE BANANA NANICÃO

2019 ◽  
Vol 2 (2) ◽  
pp. 16
Author(s):  
Eloisa Lorenzetti ◽  
Dablieny Hellen Garcia Souza ◽  
Juliano Tartaro ◽  
José Renato Stangarlin ◽  
Gilberto Costa Braga
Keyword(s):  
Rosmarinus Officinalis ◽  
Rosmarinus Officinalis L ◽  
Colletotrichum Musae

A banana apresenta elevada suscetibilidade a doenças como as podridões que diminuem o tempo de prateleira do fruto. Uma das formas de controlar as doenças seria a utilização de métodos alternativos como os extratos de plantas medicinais. Assim, o objetivo deste estudo foi verificar a ação de formulados de alecrim (Rosmarinus officinalis L.) pó e líquido no controle de antracnose causada pelo fungo Colletotrichum musae (Berk & Curt.) Von Arx. e na maturação de banana ‘Nanicão’ em pós-colheita. Foram utilizadas as concentrações 0%; 0,25%; 0,5%; 1%; 1,5% e 2% do pó solúvel de extrato de alecrim diluído em água destilada, e as concentrações 0%; 0,5%; 1%; 2%; 4% e 6% do formulado aquoso contendo 21% de resíduo seco de alecrim diluído em água destilada. Foi realizada a mensuração da área de cada fruto e avaliada a severidade e a maturação a partir de porcentagem de área dos frutos, sendo os resultados utilizados para calcular a área abaixo da curva de progresso da doença (AACPD) e a porcentagem de maturação dos frutos. Realizou-se analise de regressão a partir dos dados obtidos e pode-se verificar que para o formulado de alecrim em pó a AACPD reduziu mais de 29% e para a maturação a redução foi de 21,72%. Para o formulado líquido a redução foi de 14,31% para AACPD e cerca de 10% para maturação. Assim, concluiu-se que houve eficiência por parte dos formulados de alecrim no controle da antracnose e no aumento da vida de prateleira da banana ‘Nanicão’.

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