scholarly journals Assessment of FTA card employment for Pasteurella multocida DNA transport and detection of virulence-associated genes in strains isolated from fowl cholera in the United States

2018 ◽  
Vol 70 (6) ◽  
pp. 1855-1861 ◽  
Author(s):  
C.N. Almeida ◽  
T.Q. Furian ◽  
K.A. Borges ◽  
G. Perdoncini ◽  
M.J. Mauel ◽  
...  
Keyword(s):  
United States ◽  
Pasteurella Multocida ◽  
Virulence Genes ◽  
Genetic Material ◽  
Storage Period ◽  
The United States ◽  
Material Transport ◽  
Dna Transport ◽  
Fowl Cholera ◽  
Fta Cards

ABSTRACT Fowl Cholera (FC) is a disease caused by Pasteurella multocida. The severity of this disease is partly caused by virulence factors. Genes encoding fimbriae, capsule, sialidases and proteins for iron metabolism may be related to P. multocida’s ability to infect the host. Besides to examining DNA for the presence of virulence genes, DNA is essential for the diagnostic and FTA cards are an alternative for genetic material transport. The study aims to evaluate the viability of P. multocida DNA transport using the cards and to detect 14 virulence genes in 27 strains isolated from FC cases in the United States by multiplex-PCR. No growth was observed in any of the FTA cards, which was essential to assess the security. Furthermore, DNA detection was possible in 100% of the samples, independent of the storage period (7 to 35 days) and temperature (4°C and 37°C). ptfA, exbd-tonB, hgbA, nanB, oma87, hyaD-hyaC, sodC, hgbB, sodA, nanH and pfhA genes were detected in more than 80% of the samples. FTA cards have proven to be a viable and safe tool for DNA transport of P. multocida. A majority of genes showed a high frequency, which was similar to strains isolated from FC cases.

Detection of Virulence Plasmid–Encoded Genes in Salmonella Typhimurium and Salmonella Kentucky Isolates Recovered from Commercially Processed Chicken Carcasses

2019 ◽  
Vol 82 (8) ◽  
pp. 1364-1368 ◽  
Author(s):  
RIZWANA TASMIN ◽  
PAUL A. GULIG ◽  
SALINA PARVEEN
Keyword(s):  
United States ◽  
Salmonella Typhimurium ◽  
Virulence Genes ◽  
Salmonella Enterica Serovar Typhimurium ◽  
The United States ◽  
Clinical Isolates ◽  
Virulence Plasmid ◽  
Chicken Carcass ◽  
Serovar Typhimurium ◽  
Salmonella Virulence

ABSTRACT Salmonella enterica serovar Typhimurium is one of the leading causes of nontyphoidal gastroenteritis of humans in the United States. Commercially processed poultry carcasses are frequently contaminated with Salmonella serovar Kentucky in the United States. The aim of the study was to detect the Salmonella virulence plasmid containing the spv genes from Salmonella isolates recovered from commercially processed chicken carcasses. A total of 144 Salmonella isolates (Salmonella Typhimurium, n = 72 and Salmonella Kentucky, n = 72) were used for isolation of plasmids and detection of corresponding virulence genes (spvA, spvB, and spvC). Only four (5.5%) Salmonella Typhimurium isolates tested positive for all three virulence genes and hence were classified as possessing the virulence plasmid. All isolates of Salmonella Kentucky were negative for the virulence plasmid and genes. These results indicate that the virulence plasmid, which is very common among clinical isolates of Typhimurium and other Salmonella serovars (e.g., Enteritidis, Dublin, Choleraesuis, Gallinarum, Pullorum, and Abortusovis), may not be present in a significant portion of commercially processed chicken carcass isolates.

scholarly journals Distribution of tet(H) among Pasteurella isolates from the United States and Canada.

1996 ◽  
Vol 40 (6) ◽  
pp. 1558-1560 ◽  
Author(s):  
L M Hansen ◽  
P C Blanchard ◽  
D C Hirsh
Keyword(s):  
United States ◽  
Transposable Element ◽  
Plasmid Dna ◽  
Pasteurella Multocida ◽  
The United States ◽  
Pasteurella Haemolytica ◽  
Chromosomal Dna ◽  
H Gene

Tetracycline-resistant isolates of Pasteurella multocida and Pasteurella haemolytica obtained from various locations in the United States and Canada were studied to determine the distribution of the tet(H) gene. Of the 31 isolates examined, 25 were found to contain the tet(H) gene. Chromosomal or plasmid DNA obtained from those that did not contain the tet(H) gene did not hybridize with probes specific for classes A through G, though chromosomal DNA from one isolate lacking tet(H) hybridized with a probe specific for class M. The tet(H) gene was found on plasmid as well as on chromosomal DNA, suggesting that it is carried on a transposable element.

scholarly journals Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli, Including Sequence Type 131 (ST131), from Retail Chicken Breasts in the United States in 2013

2017 ◽  
Vol 83 (6) ◽  
Author(s):  
James R. Johnson ◽  
Stephen B. Porter ◽  
Brian Johnston ◽  
Paul Thuras ◽  
Sarah Clock ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Virulence Genes ◽  
Meat Products ◽  
The United States ◽  
Sequence Type ◽  
The Other ◽  
Antibiotic Resistant ◽  
Content Type ◽  
E Coli

ABSTRACT Chicken meat products are hypothesized to be vehicles for transmitting antimicrobial-resistant and extraintestinal pathogenic Escherichia coli (ExPEC) to consumers. To reassess this hypothesis in the current era of heightened concerns about antimicrobial use in food animals, we analyzed 175 chicken-source E. coli isolates from a 2013 Consumer Reports national survey. Isolates were screened by PCR for ExPEC-defining virulence genes. The 25 ExPEC isolates (12% of 175) and a 2:1 randomly selected set of 50 non-ExPEC isolates were assessed for their phylogenetic/clonal backgrounds and virulence genotypes for comparison with their resistance profiles and the claims on the retail packaging label (“organic,” “no antibiotics,” and “natural”). Compared with the findings for non-ExPEC isolates, the group of ExPEC isolates had a higher prevalence of phylogroup B2 isolates (44% versus 4%; P < 0.001) and a lower prevalence of phylogroup A isolates (4% versus 30%; P = 0.001), a higher prevalence of multiple individual virulence genes, higher virulence scores (median, 11 [range, 4 to 16] versus 8 [range, 1 to 14]; P = 0.001), and higher resistance scores (median, 4 [range, 0 to 8] versus 3 [range, 0 to 10]; P < 0.001). All five isolates of sequence type 131 (ST131) were ExPEC (P = 0.003), were as extensively resistant as the other isolates tested, and had higher virulence scores than the other isolates tested (median, 12 [range, 11 to 13] versus 8 [range, 1 to 16]; P = 0.005). Organic labeling predicted lower resistance scores (median, 2 [range, 0 to 3] versus 4 [range, 0 to 10]; P = 0.008) but no difference in ExPEC status or virulence scores. These findings document a persisting reservoir of extensively antimicrobial-resistant ExPEC isolates, including isolates from ST131, in retail chicken products in the United States, suggesting a potential public health threat. IMPORTANCE We found that among Escherichia coli isolates from retail chicken meat products purchased across the United States in 2013 (many of these isolates being extensively antibiotic resistant), a minority had genetic profiles suggesting an ability to cause extraintestinal infections in humans, such as urinary tract infection, implying a risk of foodborne disease. Although isolates from products labeled “organic” were less extensively antibiotic resistant than other isolates, they did not appear to be less virulent. These findings suggest that retail chicken products in the United States, even if they are labeled “organic,” pose a potential health threat to consumers because they are contaminated with extensively antibiotic-resistant and, presumably, virulent E. coli isolates.

Blasian Invasion

2017 ◽  
Author(s):  
Myra S. Washington
Keyword(s):  
United States ◽  
Asian Americans ◽  
Genetic Material ◽  
Mixed Race ◽  
The United States ◽  
Asian Ancestry ◽  
Dominant Discourses

This book examines the racialization of Blasians – mixed race people with Black and Asian ancestry – that neither sees them as new or unique, nor as a racial salve to move the United States past the problem of the colour line. The emergence of Blasian celebrities and the analyses of these stars acknowledges that to understand what and who is a Blasian means to first understand hegemonic notions of both Blacks and Asian/Americans. Contextualized against those dominant discourses Blasians explode the narrow boundaries of authenticity around racialized categories. Multiracial people are just as capable as monoracial people of upholding hierarchies of identity, as well as dismantling those hierarchies. Thus, in this book Blasians do not escape race, or erase race, but they do deconstruct normative instantiations of identity. The presence, mobility, and utility of these multiracial celebrities within both U.S. and global racial schemas simultaneously realize and complicate potential alternatives to racial and racist paradigms. These mixed race stars draw attention to how risible and absurd the biological and cultural premises for racialization truly are, and demonstrate potential alternatives for affiliation that do not rely on genetic material.

scholarly journals Draft Genome Sequence of Pasteurella multocida Isolate P1062, Isolated from Bovine Respiratory Disease

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Juan E. Abrahante ◽  
Samuel S. Hunter ◽  
Samuel K. Maheswaran ◽  
Melissa J. Hauglund ◽  
Fred M. Tatum ◽  
...  
Keyword(s):  
United States ◽  
Respiratory Disease ◽  
Genome Sequence ◽  
Pasteurella Multocida ◽  
Bovine Respiratory Disease ◽  
Draft Genome ◽  
The United States ◽  
Draft Genome Sequence

Here, we report the draft genome of Pasteurella multocida isolate P1062 recovered from pneumonic bovine lung in the United States in 1959.

scholarly journals Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR

2013 ◽  
Vol 33 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Thales Q. Furian ◽  
Karen A. Borges ◽  
Silvio L.S. Rocha ◽  
Everton E. Rodrigues ◽  
Vladimir P. do Nascimento ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
High Frequency ◽  
Respiratory Diseases ◽  
Pasteurella Multocida ◽  
Virulence Genes ◽  
Simultaneous Detection ◽  
Fowl Cholera ◽  
Avian Diseases ◽  
Genetic Profiles ◽  
Current Systems

The current systems of breeding poultry, based on high population density, increase the risk of spreading pathogens, especially those causing respiratory diseases and those that have more than one host. Fowl Cholera (FC) is one such pathogen, and even though it represents one of several avian diseases that should be considered in the differential diagnosis of notifiable diseases that present with sudden death, the pathogenesis and virulence factors involved in FC are still poorly understood. The objective of this study was to investigate twelve genes related to virulence in 25 samples of Pasteurella multocida isolated from FC cases in the southern region of Brazil through the development of multiplex PCR protocols. The protocols developed were capable of detecting all of the proposed genes. The ompH, oma87, sodC, hgbA, hgbB, exBD-tonB and nanB genes were present in 100% of the samples (25/25), the sodA and nanH genes were present in 96% (24/25), ptfA was present in 92% (23/25), and pfhA was present in 60% (15/25). Gene toxA was not identified in any of the samples studied (0/25). Five different genetic profiles were obtained, of which P1 (negative to toxA) was the most common. We concluded that the multiplex-PCR protocols could be useful tools for rapid and simultaneous detection of virulence genes. Despite the high frequency of the analyzed genes and the fact that all samples belonged to the same subspecies of P. multocida, five genetic profiles were observed, which should be confirmed in a study with a larger number of samples.

scholarly journals Genetic Characterization of Escherichia coli O104 Isolates from Different Sources in the United States

2011 ◽  
Vol 78 (5) ◽  
pp. 1615-1618 ◽  
Author(s):  
Lydia V. Rump ◽  
Sonya Bodeis-Jones ◽  
Jason Abbott ◽  
Shaohua Zhao ◽  
Julie Kase ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Virulence Genes ◽  
Genetic Characterization ◽  
The United States ◽  
Content Type ◽  
E Coli ◽  
Virulence Markers ◽  
Different Sources

ABSTRACTEscherichia coliO104 isolates collected from different sources in the United States were examined for virulence genes typical of enterohemorrhagicE. coliand those identified in the O104:H4 isolate associated with the 2011 German outbreak. The unexpected presence of virulence markers in these isolates highlights the importance of screening unusual and potentially pathogenic Shiga toxin-producingE. coliserotypes.

scholarly journals From Ashes to Diamonds

2021 ◽  
Vol 26 ◽  
pp. 122-138
Author(s):  
Filipe Calvão ◽  
Lindsay Bell
Keyword(s):  
United States ◽  
Human Body ◽  
Genetic Material ◽  
The United States ◽  
Life Forms ◽  
Life And Death ◽  
Synthetic Diamonds ◽  
Artificial Memory ◽  
Emotional Value ◽  
Living Objects

This article examines the making and makers of “memorial diamonds.” These are “natural” diamonds identical to gemstones found in nature but produced in laboratories with carbon sourced from genetic material (cremation ashes) or other objects of symbolic and emotional value. Threading corporality and objectified life forms, we examine the transformation from ashes to the “afterlife” of these “living” objects that are at once synthetic and organic. We ask, first, what material and affective properties distinguish synthetic diamonds from those extracted from nature? Second, how are these living and memorialized representations of inert substances – in continuity with bodily elements of the deceased – valued and mediated through “real” human, though artificially grown, natural objects? Drawing from research with the leading companies in the memorial diamond business in Switzerland and the United States, this article suggests that these diamonds’ singular connection to the human body offer a window into the transmutations between nature and the artificial, memory and material likeness, life and death.

scholarly journals Changes of Races and Virulence Genes in Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen, in the United States from 1968 to 2009

Plant Disease ◽  
2017 ◽  
Vol 101 (8) ◽  
pp. 1522-1532 ◽  
Author(s):  
Tinglan Liu ◽  
Anmin Wan ◽  
Dengcai Liu ◽  
Xianming Chen
Keyword(s):  
United States ◽  
Stripe Rust ◽  
Virulence Genes ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Single Gene ◽  
The United States ◽  
Wheat Cultivars ◽  
New Races ◽  
Serious Disease

Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici, is a serious disease of wheat in the world. The obligate biotrophic fungal pathogen changes its virulence rapidly, which can circumvent resistance in wheat cultivars and cause severe epidemics. Because P. striiformis f. sp. tritici races have been identified in the United States using different wheat genotypes in different time periods, it is difficult to make direct comparisons of the current population with historical populations. The objective of this study was to characterize historical populations with 18 Yr single-gene lines that are currently used to differentiate P. striiformis f. sp. tritici races in order to understand virulence and race changes of the pathogen over 40 years in the United States. From 908 P. striiformis f. sp. tritici isolates collected from 1968 to 2009 in the United States, 171 races were identified and their frequencies were determined. More races, more new races, and races with more virulence genes were detected since the year 2000 than prior to 2000. None of the races were virulent to Yr5 and Yr15, indicating that these genes have been effective since the late 1960s. Virulence genes to the remaining 16 Yr genes were detected in different periods, and most of them increased in frequency over time. Some virulence genes such as those to Yr17, Yr27, Yr32, Yr43, Yr44, YrTr1, and YrExp2 appeared 14 to 37 years earlier than previously reported, indicating the greater value of using Yr single-gene lines as differentials. Positive and negative associations were detected between virulence genes. The continual information on virulence and races in the P. striiformis f. sp. tritici populations is useful for understanding the evolution of the pathogen and for breeding wheat cultivars with effective resistance to stripe rust.

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