scholarly journals Antioxidant activity of anthocyanins from quixabeira (Sideroxylon obtusifolium) fruits

2015 ◽  
Vol 17 (3) ◽  
pp. 473-479 ◽  
Author(s):  
F.J. FIGUEIREDO ◽  
V.L.A.G. LIMA
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Skin Color ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Positive Control ◽  
Differential Method ◽  
Butylated Hydroxytoluene ◽  
Total Anthocyanins ◽  
Dpph Scavenging

ABSTRACT The fruits of Sideroxylon obtusifolium T.D. Penn. are small berries which have dark purple skin color at maturity due the anthocyanins presence. Therefore, the aim of this research was to evaluate the antioxidant activity of anthocyanins from mature fruits which were obtained at a semi-arid region of Paraiba/BR. In addition, the total anthocyanins content was measured by the pH differential method. The anthocyanins were purified by solid-phase extraction (SPE) using C18 Sep-Pak cartridges and submitted to antioxidant activity determined by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH·). The butylated hydroxytoluene (BHT) was the positive control and the results were expressed as DPPH· scavenging activity (%). Anthocyanins content were higher in the husks (236.15 mg cyanidin-3-glucoside 100g-1 fw) than in the pulp (30.49 mg cyanidin-3-glucoside 100g-1 fw). The results showed that the potential free radical scavenging grew with the increase of concentration used and the reaction time. The ability of the anthocyanins in scavenging the free radical was statistically greater than the BHT one. The anthocyanins which were present in this fruit may supply substantial dietary source of antioxidant that may promote health and produce disease prevention effects or that could be applied in food industry as a good source of natural pigments

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

Study on the Antioxidant Activities of Gel Containing Tagetes erecta (L.)

2013 ◽  
Vol 747 ◽  
pp. 741-744 ◽  
Author(s):  
Tunyaluk Limsuwan ◽  
Prapaporn Boonme ◽  
Thanaporn Amnuaikit
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Common Garden ◽  
Butylated Hydroxytoluene ◽  
Gelling Agent ◽  
Tagetes Erecta ◽  
Radical Scavenging Properties

Tagetes erecta Linn. (Asteraceae), commonly known as marigold, is a common garden plant in Thailand. It was reported that antioxidant, antimycotic, and analgesic activities were found in Tagetes erecta. In this study, the alcoholic crude extracts of flowers of Tagetes erecta were evaluated its free radical scavenging properties by using 2,2diphenyl-1-picrylhydrazyl (DPPH) free radical. Butylated hydroxytoluene (BHT) was used as standard antioxidant. Tagetes erecta extract possessed the potency of DPPH radical scavenging activity (EC50 6.56±1.46 μg/ml) more than BHT (EC50 11.06±1.75 μg/ml). Later, it was prepared in gel formulation at concentration of 0.1 % w/w Tagetes erecta extract which Carbopol Ultrez 21 or Sepigel 305 was used as a gelling agent. Propylene glycol, Uniphen P-23, triethanolamine, and distilled water were used as ingredients. The physicochemical property was evaluated in terms of physical appearances, pH, viscosity, and antioxidant activity. All formulations possessed good antioxidant activity. In addition, it has good physical appearances, and pH ranged of 4 to 6. However, the viscosity depended on a polymer type and concentration of polymer.

scholarly journals Antioxidant Activity Test of 2,6-bis-(2’-furilidyn)-Cyclohexanone, ; 2,5-bis-(2’-furilidyn)-Cyclopentanone; 1,5-Difuryl-1,4-pentadien-3-one

2010 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Ismi Rahmawati ◽  
Endang Sri Rejeki ◽  
Sardjiman Sardjiman
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Positive Control ◽  
Log P ◽  
Dpph Radical ◽  
Curcumin Analog ◽  
Activity Test ◽  
Activity Structure ◽  
Ic50 Value

Antioxidant is an essential compound to keep man’s health due to its function as radical scavenging. Curcumin analog compounds can function as antioxidant (Sardjiman, 2000). The aim of the experiment was to find out the antioxidant activity of 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one compounds, and the antioxidant activity of each compound against DPPH  radical with IC50 parameter as well as the correlation of compounds structure’s activities against antioxidant. The antioxidant activity of curcumin analog compounds wereas tested against DPPH free radical. The test was conducted in 5 series of concentrations by adding 4.0 ml test solutions with 1.0 ml DPPH. The antioxidant activity against free radical was measured usingwith spectrophotometer at 517 nm wavelength and determined for the IC50 value. The experiment employed rutin as positive control. The result of the experiment showed that curcumin analog compounds have antioxidant activity with IC50 of rutin, 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one as follows: 4.93 ppm, 22.73 ppm, 20.67 ppm, and 18.80 ppm  respectively. The highest antioxidant activity belonged to 1,.5-difuril-1.4-pentadien-3-one compound which is 18.80 ppm . Correlation of activity structure of the 3 compounds can be seen from the log p parameter and energy space of HOMO-LUMO.Key words: Antioxidant, DPPH, Analog Curcumin

scholarly journals Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay

2019 ◽  
pp. 1-6
Author(s):  
Alhassan M. Garba ◽  
Habiba R. Isa ◽  
Sadiq Abubakar ◽  
Saudat Ja’afar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Dried and powdered leaves of Albizia chevalieri were extracted using ethanol. The extract was fractionated to give methanol, chloroform and pet-ether. The four extracts obtained; ethanol, chloroform, methanol and pet-ether were evaluated for antioxidant activity using 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) free radical scavenging activity assay. The results of the DPPH scavenging activity indicated a concentration-dependent antioxidant activity. The DPPH scavenging activity of the ethanol, chloroform and methanol extracts were found to be promising. There is no significant difference in the antioxidant activity between the ethanol, chloroform and methanol extracts with that of standard Ascorbic acid at 10, 25, 250 and 500 μg/ml concentrations. This showed that the ethanol, chloroform and methanol leave extracts of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.

scholarly journals SYNTHESIS AND ANTIOXIDANT ACTIVITY OF 2-SUBSTITUTED-5-NITRO BENZIMIDAZOLE DERIVATIVES

2016 ◽  
Vol 9 (1) ◽  
pp. 308 ◽  
Author(s):  
Sabrina Rahman Archie ◽  
Biplab Kumar Das ◽  
Md. Shahadat Hossain ◽  
Uttom Kumar ◽  
Abu Shara Shamsur Rouf
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Benzimidazole Derivatives ◽  
Antioxidant Compounds ◽  
Sodium Metabisulfite ◽  
The Future ◽  
Substituted Benzaldehydes

<p><strong>Objective: </strong>This study was conducted to synthesise some 2-substituted-5-nitro benzimidazole derivatives to evaluate their antioxidant activity.</p><p><strong>Methods: </strong>The titled compounds were synthesised by sodium metabisulfite mediated reaction of 4-nitro-1, 2-phenylenediamine with a number of <em>para</em>-substituted benzaldehydes. The antioxidant activity was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.</p><p><strong>Results: </strong>All the compounds exhibited good antioxidant activity having IC<sub>50</sub> values in the range of 3.17 to 7.59 µg/ml while that of standard butylated hydroxytoluene (BHT) was 18.42 µg/ml.</p><strong>Conclusion: </strong>Among the synthesised compounds, compounds 3a-c have been shown to possess the most prominent antioxidant activity which might be attributed due to the presence of chloro, bromo and fluoro substituents in the molecule. The compounds may act as the future lead(s) for the development of potential antioxidant compounds.<p> </p>

scholarly journals Free radical scavenging activity of taro (Colocasia esculenta (L.) Schott) varieties with varying flesh color

2020 ◽  
pp. 23-32
Author(s):  
Erlinda Vasquez ◽  
Precila Contero ◽  
Dilberto Ferraren
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reduction Rate ◽  
Free Radical Scavenging ◽  
Differential Method ◽  
Free Radical Scavenging Activity ◽  
Antioxidant Compounds ◽  
Scavenging Activity

Corms of five taro varieties namelyztwo NSIC-registered varieties, VG-1 (Kalpao) and VG-2 (Iniito), and three farmer’s variety, PRG 322 (Chowking), PRG 381 (Pandaga), and PRG 380 (Salot), were evaluated for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) assay before and after cooking. The purple-fleshed varieties PRG 322, PRG 380 and VG-2 showed higher antioxidant activity than theyellow- and cream-fleshed varieties, The monomeric anthocyanin contents of PRG 380 and VG-1 PRG 322 and VG-2, which showed the highest free radical scavenging activity were measured using the pH differential method. The free» radical scavenging activity ofthe five taro varieties ranged from 134 to 154TEμm g-1 when raw and 127 to 147TEμm g-1 when cooked with the highest activity in raw PRG 322 followed by raw PRG 380 and VG-2. Cooked PRG 381 and VG-1 showed the lowest activity, Cooking reduced the activity by 4.5-10.2% with PRG 380 showing the highest reduction rate while the other four varieties did not vary significantly from 4.5-5.9%. Decreased in antioxidant activity can be due to the degradation and leaching out of antioxidant compounds during steamed cooking.

Antioxidant p-terphenyl compound, isolated from edible mushroom, Boletopsis leucomelas

2021 ◽  
Author(s):  
Ferry Ferdiansyah Sofian ◽  
Nodoka Kikuchi ◽  
Takuya Koseki ◽  
Yuko Kanno ◽  
Shota Uesugi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Spectral Analysis ◽  
Free Radical ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Scavenging

Abstract A new acetyl p-terphenyl derivative, boletopsin 15, was isolated from the ethyl acetate extract of fruit bodies of the Basidiomycete Boletopsis leucomelas, together with four known compounds. The structures of these compounds were elucidated by spectral analysis, as well as by directly comparing the spectral data of the new compound with those of known compounds. The free radical-scavenging activity of the compounds was assayed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The results showed that compounds 1 and 2 exhibited significant antioxidant activity (1: EC50 = 2.1 μM and 2: EC50 = 6.6 μM).

scholarly journals Utilization of tomato waste as a source of polyphenolic antioxidants

2010 ◽  
pp. 187-194 ◽  
Author(s):  
Sladjana Savatovic ◽  
Gordana Cetkovic ◽  
Jasna Canadanovic-Brunet ◽  
Sonja Djilas
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Ultrasonic Bath ◽  
Tomato Waste ◽  
Flavonoid Fraction

This study is concerned with the effects of two extraction procedures (using ultrasonic bath and high performance homogenizer) on the extraction efficiency of polyphenolics present in the tomato waste. The isolation of flavonoid fraction of obtained extracts was performed by solid-phase extraction. The antioxidant activity of flavonoid fractions was determined using different spectrophotometric tests, including reducing power and 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. The content of total polyphenolics and flavonoids in extract obtained employing homogenizer (E2) was higher than in the extract obtained employing ultrasonic bath (E1), and it was 14.33 mg/g and 7.70 mg/g, respectively. The flavonoid fraction (EF2) of extract E2 showed higher antioxidant activity than flavonoid fraction (EF1) of extract E1. The DPPH free radical scavenging activity of fractions EF1 and EF2, expressed as EC50 value, were 0.78 mg/ml and 0.45 mg/ml, respectively. The obtained results show that tomato wastes can be used as an easily accessible source of antioxidant polyphenolics.

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1325
Author(s):  
Mohamed Z. M. Salem ◽  
Abeer A. Mohamed ◽  
Hayssam M. Ali ◽  
Dunia A. Al Al Farraj
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Leaf Extracts ◽  
Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

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