scholarly journals Antioxidant Activity Test of 2,6-bis-(2’-furilidyn)-Cyclohexanone, ; 2,5-bis-(2’-furilidyn)-Cyclopentanone; 1,5-Difuryl-1,4-pentadien-3-one

2010 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Ismi Rahmawati ◽  
Endang Sri Rejeki ◽  
Sardjiman Sardjiman
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Positive Control ◽  
Log P ◽  
Dpph Radical ◽  
Curcumin Analog ◽  
Activity Test ◽  
Activity Structure ◽  
Ic50 Value

Antioxidant is an essential compound to keep man’s health due to its function as radical scavenging. Curcumin analog compounds can function as antioxidant (Sardjiman, 2000). The aim of the experiment was to find out the antioxidant activity of 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one compounds, and the antioxidant activity of each compound against DPPH  radical with IC50 parameter as well as the correlation of compounds structure’s activities against antioxidant. The antioxidant activity of curcumin analog compounds wereas tested against DPPH free radical. The test was conducted in 5 series of concentrations by adding 4.0 ml test solutions with 1.0 ml DPPH. The antioxidant activity against free radical was measured usingwith spectrophotometer at 517 nm wavelength and determined for the IC50 value. The experiment employed rutin as positive control. The result of the experiment showed that curcumin analog compounds have antioxidant activity with IC50 of rutin, 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one as follows: 4.93 ppm, 22.73 ppm, 20.67 ppm, and 18.80 ppm  respectively. The highest antioxidant activity belonged to 1,.5-difuril-1.4-pentadien-3-one compound which is 18.80 ppm . Correlation of activity structure of the 3 compounds can be seen from the log p parameter and energy space of HOMO-LUMO.Key words: Antioxidant, DPPH, Analog Curcumin

scholarly journals ANTIOXIDANT ACTIVITY AND CARDIOPROTECTIVE ACTIVITY OF BANGUN-BANGUN LEAVES (PLECTRANTHUS AMBOINICUS LOUR.) ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (9) ◽  
pp. 165
Author(s):  
Modesta Harmoni Tarigan ◽  
Urip Harahap ◽  
Aminah Dalimunthe ◽  
Nerdy Nerdy
Keyword(s):  
Antioxidant Activity ◽  
Troponin T ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Heart Tissue ◽  
Positive Control ◽  
Control Group ◽  
Activity Test ◽  
Ic50 Value ◽  
Plectranthus Amboinicus

Objectives: The objectives of this study were to determine the antioxidant activity and cardioprotective activity of bangun-bangun leaves ethanolic extract.Methods: Bangun-bangun leaves ethanolic extract was obtained by maceration process. The antioxidant activity test was performed by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging method with various concentrations of extract. The absorbance was measured by visible spectrophotometric method and calculated the inhibitory concentration (IC50) value for antioxidant activity analysis. Cardioprotective activity test was performed by measuring the cardiac troponin T (cTnT) level, creatine kinase-muscle/brain (CK-MB) level, and histology of the heart tissue. Animals induced with doxorubicin at the 8th day and the 9th day, bangun-bangun leaves ethanolic extract was administered from the 1st day to the 9th day with various doses of extract.Results: Bangun-bangun leaves ethanolic extract had IC50 value of 57.79 μg/mL. Difference dose of bangun-bangun leaves ethanolic extract shows difference cardioprotective activity. Bangun-bangun leaves ethanolic extract at dose 300 mg/kg bw did not differ significantly to the positive control group and normal group. The higher the dose of an extract the greater the decrease in cTnT and CK-MB levels and increase protection against heart damage.Conclusion: Bangun-bangun leaves ethanolic extract had strong antioxidant and had cardioprotective activity. 

scholarly journals ANTIOXIDANT ACTIVITY OF METHYL GALLATE ISOLATED FROM THE LEAVES OF Toona sureni

2010 ◽  
Vol 9 (3) ◽  
pp. 457-460 ◽  
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methyl Gallate ◽  
Antioxidant Compound ◽  
Ic50 Value ◽  
Isolated Compound ◽  
Dpph Radical Scavenging

An antioxidant compound has been isolated from the leaves of Toona sureni (Blume) Merr. The structure was determined to be methyl 3,4,5-trihydroxybenzoate (methyl gallate), based on UV-vis, FTIR, NMR and MS spectra. The isolated compound exhibited potent antioxidant activity in the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging test, with IC50 value 1.02 μg/mL.   Keywords: antioxidant activity, methyl gallate, Toona sureni

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

Antioxidant Activity of Selected Thyme (Thymus L.) Species and Study of the Equivalence of Different Measuring Methodologies

2015 ◽  
Vol 98 (4) ◽  
pp. 876-882 ◽  
Author(s):  
Marta Orłowska ◽  
Teresa Kowalska ◽  
Mieczysław Sajewicz ◽  
Katarzyna Pytlakowska ◽  
Mariola Bartoszek ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Resonance Spectroscopy ◽  
Dpph Radical ◽  
Dot Blot ◽  
Phenolic Fraction ◽  
Measuring Techniques

Abstract This study presents the results of comparative evaluation of the antioxidant activity of the phenolic fraction exhaustively extracted with aqueous methanol from 18 different thyme (Thymus L.) specimens and species. This evaluation is made with use of the same free radical source (DPPH• radical), three different free radical scavenging models (gallic acid, ascorbic acid, and Trolox), and three different measuring techniques (the dot blot test, UV-Vis spectrophotometry, and electron paramagnetic resonance spectroscopy, EPR). A comparison of the equivalence of these three different measuring techniques (performed with use of hierarchical clustering with Euclidean distance as a similarity measure and Ward's linkage) is particularly important in view of the fact that different laboratories use different antioxidant activity measuring techniques, which makes any interlaboratory comparison hardly possible. The results obtained confirm a semiquantitative equivalence among the three compared methodologies, and a proposal is made of a simple and cost-effective dot blot test that uses the DPPH• radical and provides differentiation of antioxidant activity of herbal matter comparable with the results of the UV-Vis spectrophotometry and EPR.

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals ANTIOXIDANT ACTIVITIES OF HYDROLYSATES ORIGINATED FROM SOYBEAN AND SOY MILK RESIDUE

2018 ◽  
Vol 55 (5A) ◽  
pp. 134
Author(s):  
Ngo Minh Ngoc
Keyword(s):  
Antioxidant Activity ◽  
Protein Content ◽  
Manufacturing Industry ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging ◽  
Soy Milk ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Ic50 Values

Soybean (Glycine max) and soy milk residue (okara) are protein-rich materials. Soybean possesses the highest protein content among different types of beans (protein content of soybean varies from 40–42 %). Soy milk residue, a by-product of the soy milk manufacturing industry, contains approximately 27 % protein (by dry weight). A number of recent studies have investigated the improvement of functional properties of protein contained in soybean and okara by fermentation or by the use of proteolytic enzymes. The aim of this study was to evaluate the antioxidant activities of soybean and okara hydrolysates obtained by the fermentation with Aspergillus oryzae or by using proteolytic enzymes (neutrase and flavourzyme). DPPH radical scavenging assay was used to determine the antioxidant activities of hydrolysates. The concentration of peptides required to scavenge DPPH radical by 50 % (IC50 value) was used to evaluate the antioxidant activity of peptides produced obtained from hydrolysates. The results showed that when fermented with A. oryzae, the okara hydrolysate had higher antioxidant activity than the soybean hydrolysate, with IC50 values of 0.447 mg/ml and 3.95 mg/ml, respectively. The hydrolyzed okara obtained from hydrolysis using Neutrase had higher antioxidant activity than the one obtained from hydrolysis using Flavourzyme, with IC50 values of0.200 mg/ml and 0.407 mg/ml, respectively. Different peptide fractions obtained from the hydrolysates using cut-off membrane (10 kDa, 3 kDa and 1 kDa) possessed different antioxidant activities. The < 1 kDa peptide fraction exhibited the highest antioxidant activity with an IC50 value of 0.158 mg/ml.

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK TUMBUHAN PAKU EKOR KUDA (Equisetum debile L.) TERHADAP PEROKSIDASI LIPID PLASMA DARAH MENCIT (Mus musculus)

2017 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Riana Dyah Suryaningrum ◽  
Ni Made Puspawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Free Radical ◽  
Blood Plasma ◽  
Mus Musculus ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Activity Test ◽  
Dpph Method

The purpose of this research was to study the antioxidant activity from ethanol extract of horstail (Equisetum debile L.) in the free radical scavenging in mice blood plasma. The antioxidant activity test was conducted with the DPPH method and measuring the MDA concentration in mice blood. The antioxidant activity test with the DPPH results showed that the ethanol extract of horstail (Equisetum debile L.) had the antioxidant activity of IC50 which was 1.604 mg/mL or 1,604 ppm. The statistical analysis result of the MDA blood plasma in mice with various doses (125 mg/kgBM, 250 mg/kgBM, 375 mg/kgBM and 500 mg/kgBM) showed that the 500 mg/kgBM dose extract was able to reduce the MDA concentration in mice blood which given the most amount of exercise.

EVALUATION OF ANTIOXIDANT ACTIVITY OF UNRIPE AEGLE MARMELOS CORR. FRUITS

2018 ◽  
pp. 4-7
Author(s):  
Gupta D ◽  
John PP ◽  
Kumar P ◽  
Jain J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Aegle Marmelos ◽  
Indian Origin ◽  
Ic50 Value ◽  
Phytochemical Compounds ◽  
Free Radical Scavenging Assay

Aegle marmelos Corr. (Rutaceae), commonly known as Bael, is a tree of Indian origin, well known from ancient period andprescribed for various ailments in Ayurveda. Utilization of bael fruit in day-to-day life has a great nutritional, environmental as well ascommercial importance. Every part of Aegle marmelos including stem, bark, root, leaves, fruit and seeds at all stages of maturity possesmedicinal virtues and has been used in Ethno medicine to exploit its medicinal properties. Objective: This study was undertaken to examine theantioxidant activity of methanolic extract of Aegle marmelos unripe or half ripe fruits. Material and Methods: The antioxidant activity was doneby using DPPH free radical scavenging assay. The IC50 (The concentration of sample required to scavenge 50% of DPPH free radical) wascalculated by plotting graph between % inhibition vs concentration. The ascorbic acid was used as standard antioxidant. Result and Discussion:The IC50 value of extract and ascorbic acid was found to be 62.59μg/ml and 2.80μg/ml. The antioxidant activity found in Aegle marmelos maybe associated with their main phytochemical compounds like flavonoids, phenols and tannins. Conclusion: This activity supports that the fruitcan be used as natural antioxidant to treat free radical induced cellular damages and can also be used as adjuvant with other drugs to givesynergistic effects.

scholarly journals Antioxidant activity of anthocyanins from quixabeira (Sideroxylon obtusifolium) fruits

2015 ◽  
Vol 17 (3) ◽  
pp. 473-479 ◽  
Author(s):  
F.J. FIGUEIREDO ◽  
V.L.A.G. LIMA
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Skin Color ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Positive Control ◽  
Differential Method ◽  
Butylated Hydroxytoluene ◽  
Total Anthocyanins ◽  
Dpph Scavenging

ABSTRACT The fruits of Sideroxylon obtusifolium T.D. Penn. are small berries which have dark purple skin color at maturity due the anthocyanins presence. Therefore, the aim of this research was to evaluate the antioxidant activity of anthocyanins from mature fruits which were obtained at a semi-arid region of Paraiba/BR. In addition, the total anthocyanins content was measured by the pH differential method. The anthocyanins were purified by solid-phase extraction (SPE) using C18 Sep-Pak cartridges and submitted to antioxidant activity determined by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH·). The butylated hydroxytoluene (BHT) was the positive control and the results were expressed as DPPH· scavenging activity (%). Anthocyanins content were higher in the husks (236.15 mg cyanidin-3-glucoside 100g-1 fw) than in the pulp (30.49 mg cyanidin-3-glucoside 100g-1 fw). The results showed that the potential free radical scavenging grew with the increase of concentration used and the reaction time. The ability of the anthocyanins in scavenging the free radical was statistically greater than the BHT one. The anthocyanins which were present in this fruit may supply substantial dietary source of antioxidant that may promote health and produce disease prevention effects or that could be applied in food industry as a good source of natural pigments

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

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