REACTION OF PSIDIUM SPP. ACCESSIONS TO DIFFERENT LEVELS OF INOCULATION WITH MELOIDOGYNE ENTEROLOBII

ABSTRACT Meloidogyne enterolobii associated with Fusarium solani causes the guava decay, which is the main factor of yield loss in guava crops and limits guava production in Brazil. Therefore, searching for guava genotypes (Psidium spp.) with resistance to M. enterolobii is important to control this root-knot nematode. The objective of the present work was to evaluate the reaction of Psidium spp. accessions from the Germplasm Bank of the Bahia State University (UNEB) to different levels of inoculation with M. enterolobii. Guava seedlings were inoculated with 600, 1,600, and 2,000 eggs + J2 second stage juveniles of M. enterolobii and the root fresh weight, total number of eggs (TNE), and reproduction factor (RF) were evaluated. The results were subjected to ANOVA and means were clustered using the Scott-Knott test at 5% probability. The diversity of accessions was estimated using the Tocher's clustering and UPGMA methods. The nematode RF reduced in some accessions with increasing inoculation levels of the nematode. However, the RF increased in some accessions with high levels of inoculation. Plants of the Y50 accession were immune or resistant to M. enterolobii, indicating variability of reaction of plants to the nematode within the Psidium genus. The resistance of Psidium accessions to M. enterolobii needs to be measured with different inoculation levels to verify the existence of false-positive results, since the evaluated accessions, including the Paluma cultivar, presented distinct reactions regarding RF in the three levels of inoculation used.

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Reaction of potential guava rootstocks to Meloidogyne enterolobii

Revista CERES ◽

10.1590/0034-737x201865030010 ◽

2018 ◽

Vol 65 (3) ◽

pp. 291-295 ◽

Cited By ~ 2

Author(s):

Fernando Marcelo Chiamolera ◽

Antonio Baldo Geraldo Martins ◽

Pedro Luiz Martins Soares ◽

Tatiana Pagan Loeiro da Cunha-Chiamolera

Keyword(s):

Root System ◽

Fusarium Solani ◽

Root Rot ◽

System Development ◽

Control Treatment ◽

Root Knot Nematode ◽

Second Stage ◽

Root System Development ◽

Meloidogyne Enterolobii ◽

Reproduction Factor

ABSTRACT Root-knot nematode Meloidogyne enterolobii is the main phytosanitary problem of guava cultivation in Brazil. Among the strategies to manage the problem, the best prospects are in identifying or developing cultivars or rootstocks that are resistant to this nematode. To identify plants with potential as rootstocks for guava, the reaction of araçá (wild guava) to M. enterolobii was assessed in a greenhouse experiment. Seven araçá species were evaluated (Eugenia stipitata, Psidium acutangulum, P. cattleyanum ‘yellow’, P. friedrichsthalianum, P. guajava var. minor, P. guineense, and Psidium sp.). The plants were inoculated with a suspension of 3,000 eggs of M. enterolobii, using eggplant as control treatment. The parameters fresh root mass, number of eggs and second stage juveniles (J2) per root system, the reproduction factor (RF = Pf/Pi), and araçá reaction were determined during the experiment. RF of the araçá species E. stipitata, P. cattleyanum ‘yellow’, and P. friedrichsthalianum was less than one (RP < 1), therefore resistant to M. enterolobii. The araçá trees had good root system development and the susceptible plants showed many root galls, high number of eggs and J2, and Fusarium solani and Rhizoctonia solani root rot. The araçá species, P. cattleyanum ‘yellow’, P. friedrichsthalianum, and E. stipitata are resistant to M. enterolobii and can be tested as potential guava rootstocks.

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Resistance sources to root-knot nematode Meloidogyne enterolobii in Solanum species

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i4.2096 ◽

2020 ◽

pp. 303

Author(s):

Jadir Borges Pinheiro ◽

Giovani Olegario da Silva ◽

Jhenef Gomes de Jesus ◽

Danielle Biscaia ◽

Raphael Augusto de Castro e Melo

Keyword(s):

Solanum Species ◽

Experimental Unit ◽

Egg Mass ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Second Stage ◽

Randomized Design ◽

Meloidogyne Enterolobii ◽

Plant Grown ◽

Reproduction Factor

The objective of this work was to prospect sources of resistance to root-knot nematode Meloidogyne enterolobii in Solanum species with potential to be used as rootstocks for cultivated Solanaceae. Nine accessions of Solanum sessiliflorum, 27 accessions of S. lycocarpum, 21 accessions of S. acanthodes, 22 accessions of S. scinericum and 26 accessions of S. scuticum for resistance to M. enterolobii. Rutgers and Nemadoro tomatoes were used as susceptible and resistant controls, respectively. The experiment was conducted in a greenhouse at Embrapa Vegetables, Brasília-DF, Brazil, in a completely randomized design with six replications. The experimental unit was a represented by a single plant grown in a plastic pot containing 3 L of substrate. 4000 eggs and eventual juveniles of second stage M. enterolobii were inoculated per pot. At 119 days after inoculation, gall index (Gi), egg mass index (EMI), number of eggs per root gram (NE) and reproduction factor (Fr) were evaluated. Data were subjected to analysis of variance and grouping of treatments by Scott-Knott. It was verified that S. acanthodes and S. Lycocarpum are species with high resistance to M. enterolobii, with accessions being classified identified as immune. S. scuticum also has great potential, as several resistant accessions were identified, although some accessions were quite susceptible; whereas for S. subinerme only 4 resistant accessions were identified, although all others presented a reproduction factor much lower than tomato cv. Nemadoro as control; and all evaluated S. sessiliflorum accessions were susceptible.

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Screening Sweetpotato Genotypes for Resistance to a North Carolina Isolate of Meloidogyne enterolobii

Plant Disease ◽

10.1094/pdis-02-20-0389-re ◽

2020 ◽

Author(s):

Tanner Schwarz ◽

Eric Davis ◽

G. Craig Yencho ◽

Kenneth Pecota ◽

Chunying Li ◽

...

Keyword(s):

North Carolina ◽

Ipomoea Batatas ◽

Post Inoculation ◽

State University ◽

Root Knot Nematode ◽

Soil Mixture ◽

Initial Inoculum ◽

Meloidogyne Enterolobii ◽

Nematode Eggs ◽

Reproduction Factor

Potential resistance to the guava root-knot nematode, Meloidogyne enterolobii, in ninety-one selected sweetpotato [Ipomoea batatas (L.) Lam.] genotypes was evaluated in six greenhouse experiments. Ten thousand eggs of M. enterolobii were inoculated on each sweetpotato genotype grown in a 3:1 sand to soil mixture. Sixty days post inoculation, percent of total roots with nematode-induced galls was determined, and nematode eggs were extracted from roots. Significant differences (P ˂ 0.001) among sweetpotato genotypes were found in all six tests for gall rating, total eggs, and eggs per gram of root. Resistant sweetpotato genotypes were determined by final eggs per root system divided by the initial inoculum where Pf/Pi < 1 (reproduction factor; final egg count divided by initial inoculum of 10,000 eggs), and statistical mean separations were confirmed by Fisher’s LSD t test. Our results indicated that 19 out of 91 tested sweetpotato genotypes were resistant to M. enterolobii. Some of the susceptible genotypes included ‘Covington’, ‘Beauregard’, ‘NCDM04-001’, and ‘Hernandez’. Some of the resistant sweetpotato genotypes included ‘Tanzania’, ‘Murasaki-29’, ‘Bwanjule’, ‘Dimbuka-Bukulula’, ‘Jewel’, and ‘Centennial’. Most of the 19 resistant sweetpotato genotypes supported almost no M. enterolobii reproduction with less than 20 eggs/g root of M. enterolobii. A number of segregants from a ‘Tanzania’ x ‘Beauregard’ cross demonstrated strong resistance to M. enterolobii observed in the ‘Tanzania’ parent. In collaboration with NC State University sweetpotato breeding program, several of the genotypes evaluated in these tests are now being used to incorporate the observed resistance to M. enterolobii into commercial sweetpotato cultivars.

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Respuesta del perejil crespo a diferentes densidades de población de Meloidogyne arenaria

Revista de Investigaciones Altoandinas - Journal of High Andean Research ◽

10.18271/ria.2021.226 ◽

2021 ◽

Vol 23 (1) ◽

pp. 55-60

Author(s):

Leslie Sharon Lozada-Villanueva ◽

◽

Teodocia Gloria Casa-Ruiz ◽

Cristiano Bellé ◽

◽

...

Keyword(s):

Population Density ◽

Initial Population ◽

Fresh Weight ◽

Root Knot Nematode ◽

Initial Population Density ◽

Second Stage ◽

Leaf Weight ◽

Gall Index ◽

Reproduction Factor ◽

Curly Leaf

The effect of initial population density (Pi) of the peanut root-knot nematode, Melodoigyne arenaria, on curly leaf parsley growth was assessed in this study. The population densities of M. arenaria ranged from 0 to 64 eggs + second-stage juveniles (J2)/cm3 soil in sterile sandbags. The root gall index (RGI), reproduction factor (RF), fresh leaf weight (FLW), dry leaf weight (DLW), root fresh weight (RFW), root length (RL), leaf height (LH), and chlorophyll index (SPAD) were determined at 90 days after inoculation. FLW, DLW, RFW, LH, and SPAD data were fitted to the Seinhorst equation, y = m + (1 - m) zPi-T, to determine the tolerance limit T = 0.25 eggs +J2/cm3 soil for FLW, DLW, RFW, and LH, with relative means (m) of 0.52, 0.24, 0.22, and 0.4 respectively; conversely, the T value for SPAD was 0.125 eggs + J2/cm3 soil and with a m of 0.26. All biometric variables decreased with an increase in the initial population density (Pi). Nevertheless, the highest RF of M. arenaria in parsley was 78.92 for a Pi = 8 eggs + J2/cm3 soil, with an RGI value of 5 from Pi = 1 eggs + J2/cm3. Curly leaf parsley growth decreased with an increase in Pi of M. arenaria.

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Do the stage of plant and initial population of root knot nematode influence on sweet pepper development?

O Biológico ◽

10.31368/1980-6221v82a10018 ◽

2020 ◽

Vol 82 (1) ◽

pp. 1-10

Author(s):

Samara A. de Oliveira ◽

Juliana M. O Rosa ◽

Juliana Eulálio ◽

Claudio Marcelo G. de Oliveira

Keyword(s):

Sweet Pepper ◽

Initial Population ◽

Root Knot Nematode ◽

One Pot ◽

Leaf Pair ◽

Final Population ◽

Randomized Design ◽

Meloidogyne Enterolobii ◽

Shoot Weight ◽

Reproduction Factor

The aim of this study was to investigate the response of three different stages (one, three and five leaf pairs) of sweet pepper (Capsicum annuum cv. Orazio) seedlings to five inoculation levels of Meloidogyne enterolobii (zero (control), 300, 1000, 3000 and 10000) under greenhouse conditions. Each plant was cultivated in one pot filled with 3.8 L of substrate. The test was a completely randomized design with four replications. The plants were assessed 60 days after inoculation, plant shoot weight, final population of nematodes, and reproduction factor were measured. The results were fitted to Seinhorst model: Y = m + (1-m). ZPi –T. The results showed a tolerance limit (T) of 2,500 nematodes for plants with one and three leaf pair, and 8,500 nematodes for the five-leaf pair plant.

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Reproduction of Meloidogyne enterolobii in lettuce cultivars of the American group

Horticultura Brasileira ◽

10.1590/s0102-053620150000200002 ◽

2015 ◽

Vol 33 (2) ◽

pp. 147-150

Author(s):

Érika CSS Correia ◽

Norberto Silva ◽

Marylia GS Costa ◽

Silvia RS Wilcken

Keyword(s):

Reproductive Potential ◽

Egg Mass ◽

Root Knot Nematodes ◽

Second Stage ◽

American Group ◽

Meloidogyne Enterolobii ◽

Meloidogyne Spp ◽

Gall Index ◽

Summer Time ◽

Reproduction Factor

Lettuce is the main vegetable cultivated in Brazil, in volume and in marketed value. There are ranges of diseases which may affect lettuce crops, including those caused by root-knot nematodes (Meloidogyne spp.). We evaluated the reproductive potential of Meloidogyne enterolobii in 22 lettuce cultivars. The plants were inoculated with 5,000 eggs and eventuals second stage juveniles of nematode per pot, two days after the transplanting. 'Rutgers' tomato was used as standard for inoculum viability of M. enterolobii. We evaluated, 60 days after inoculation, the gall index, egg mass index and reproduction factor (RF). The 'Ithaca', 'Raider Plus', 'RS-1397', 'L-104', 'Challenge', 'IP-11', 'Classic', 'Salinas 88', 'Vanguard 75', 'Calona' and 'Desert Queen' were resistant to M. enterolobii with RF varying from 0.22 to 0.9, and the cultivars Lady Winterset, Robinson, Sonoma, Raider, Lucy Brown, Bnondaga, Summer Time, Taina, Sundevil and L-109 were susceptible to this nematode, with RF ranging from 1.06 to 5.73.

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Quantification of the damage caused by Meloidogyne enterolobii in okra

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2019.v54.00050 ◽

2019 ◽

Vol 54 ◽

Author(s):

Edgard Henrique Costa Silva ◽

Renato Silva Soares ◽

Heloísa Oliveira Borges ◽

Carolina Andrade Franco ◽

Leila Trevisan Braz ◽

...

Keyword(s):

Agronomic Traits ◽

Abelmoschus Esculentus ◽

Second Stage ◽

Randomized Design ◽

Meloidogyne Enterolobii ◽

Completely Randomized Design ◽

Suitable Measure ◽

Reproduction Factor ◽

Selection Of

Abstract: The objective of this work was to estimate the damage caused by Meloidogyne enterolobii in okra (Abelmoschus esculentus) and to verify the reliability of the reproduction factor as a suitable measure for selecting resistant okra genotypes. Increasing populations of M. enterolobii - 0, 500, 1,500, 3,000, 5,000, and 7,000 eggs and second-stage juveniles (J2) per plant, - were evaluated, in a completely randomized design. The pathogen showed a parasitism pattern similar to that of M. incognita, causing a significant decrease in morphological and agronomic traits. The pathogen reproduction factor should be used in the selection of okra genotypes for tolerance to M. enterolobii, in populations above 3,000 eggs or J2.

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Reproduction of Meloidogyne enterolobii in rootstocks and cucumber hybrids

Horticultura Brasileira ◽

10.1590/s0102-05362013000400018 ◽

2013 ◽

Vol 31 (4) ◽

pp. 618-621 ◽

Cited By ~ 1

Author(s):

Silvia Renata S Wilcken ◽

Juliana MO Rosa ◽

Juliana N Westerich ◽

Maria José de M Garcia ◽

Antonio Ismael Inácio Cardoso

Keyword(s):

Experimental Design ◽

Initial Population ◽

Second Stage ◽

Final Population ◽

Meloidogyne Enterolobii ◽

Reproduction Factor

This study aimed to determine the resistance of six rootstocks for cucumber (squash 'Menina Brasileira', pumpkin 'Exposição', 'Shelper', 'Tetsukabuto Takaiama', 'B8-A Tetsukabuto' and 'Excite Ikki') and four Japanese cucumber hybrids ('Yoshinari', 'Kouki', 'Taisho' and 'Tsuyataro') to Meloidogyne enterolobii. The experiment was conducted in greenhouse; each plot consisted of one plant per pot containing 2 liters of autoclaved soil. The experimental design was completely randomized, with five replicates per treatment. Two days after transplanting the seedlings, each plant was inoculated with 5,000 eggs and second-stage juveniles (initial population = Pi) of M. enterolobii. Tomato 'Rutgers' was used as indicative of inoculum viability. Sixty days after inoculated, each plant was evaluated for total number of nematodes in the roots (final population = Pf) and reproduction factor (FR=Pf/Pi). All rootstocks and cucumber hybrids allowed M. enterolobii multiplication, but FR values were lower in 'Shelper', 'Excite Ikki KY' and 'Menina Brasileira' rootstocks. In a second experiment, the low FR in these three rootstocks was confirmed, but with higher values in 'Menina Brasileira'. So, these rootstocks can be recommended to M. enterolobii infested areas, but with low populations, because they don't permit great multiplication of the patogen. It is concluded that 'Shelper' and 'Excite Ikki KY' rootstocks had the lowest FR and can be classified as moderate resistant to this nematode.

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The effect of sandy soil, bacterium dose and time on the efficacy of Pasteuria penetrans to control Meloidogyne incognita race 1 on coffee

Nematology ◽

10.1163/156854107782331171 ◽

2007 ◽

Vol 9 (6) ◽

pp. 845-851 ◽

Cited By ~ 1

Author(s):

Maria Célia Cordeiro ◽

Regina Carneiro ◽

Pedro Cirotto ◽

Luiz de Mesquita ◽

Maria Ritta Almeida ◽

...

Keyword(s):

Biological Control ◽

Sandy Soil ◽

Meloidogyne Incognita ◽

Pasteuria Penetrans ◽

Root Knot Nematode ◽

Nematode Reproduction ◽

Second Stage ◽

Race 1 ◽

Coffee Plants ◽

Reproduction Factor

AbstractAn obligate parasite bacterium of the root-knot nematode, Pasteuria penetrans strain P10, isolated from Meloidogyne incognita females on banana roots in Imperatriz Maranhão State, Brazil, was evaluated in glasshouse conditions, using two doses of a dry root bionematicide (107 endospores (5.0 g/seedling) and 106 endospores (0.5 g/seedling)) on seedlings of cv. Mundo Novo coffee. The soil in which coffee seedlings were raised was inoculated previously with these two doses of P. penetrans and after 2 months the plants were transferred to soils of different textures: clay-sandy soil (38% clay, 2% silt and 60% sand) and sandy soil (17% clay, 0% silt and 83% sand). When the coffee plants were 30 cm high, they were inoculated with 20 000 eggs/plant of M. incognita race 1. The coffee plants were examined 8, 16 and 24 months after nematode plant infestation. The effectiveness of the biological control was determined by the reduction of nematode reproduction factor, which ranged from 62 to 67% in clay-sandy soil and 80 to 85% in sandy soil. The mechanism of suppression caused by the bacterium was evaluated by the percentage of infected second-stage juveniles (J2), number of endospores attached/J2 and number of infected females. The high levels of suppression were related to time, increasing from 8 to 24 months, and to the percentage of sand in the soil.

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