Antioxidative stress effects of Humulus japonicus extracts on neuronal PC12 cells

Oxidative stress is regarded as a mediator of nerve cell death in several neurodegenerative disorders, such as Parkinson's disease. Sesamin, a lignan mainly found in sesame oil, is currently under study for its anti-oxidative and possible neuroprotective properties. We used 1-methyl-4-phenyl-pyridine (MPP+) ion, the active metabolite of the potent parkinsonism-causing toxin 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, to produce oxidative stress and neurodegeneration in neuronal PC12 cells, which express dopamine, as well as neurofilaments. Our results show that picomolar doses of sesamin protected neuronal PC12 cells from MPP+-induced cellular death, as revealed by colorimetric measurements and production of reactive oxygen species. We also demonstrated that sesamin acted by rescuing tyrosine hydroxylase levels from MPP+-induced depletion. Sesamin, however, did not modulate dopamine transporter levels, and estrogen receptor-alpha and -beta protein expression. By examining several parameters of cell distress, we found that sesamin also elicited a strong increase in superoxide dismutase activity as well as protein expression and decreased catalase activity and the MPP+stimulated inducible nitric oxide synthase protein expression, in neuronal PC12 cells. Finally, sesamin possessed significant anti-inflammatory properties, as disclosed by its potential to reduce MPP+-induced interleukin-6 mRNA levels in microglia. From these studies, we determined the importance of the lignan sesamin as a neuroprotective molecule and its possible role in complementary and/or preventive therapies of neurodegenerative diseases.

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The dynamic pattern of glucocorticoid receptor-mediated transcriptional responses in neuronal PC12 cells

Journal of Neurochemistry ◽

10.1111/j.1471-4159.2006.04187.x ◽

2006 ◽

Vol 99 (4) ◽

pp. 1282-1298 ◽

Cited By ~ 39

Author(s):

M. C. Morsink ◽

M. Joëls ◽

R. A. Sarabdjitsingh ◽

O. C. Meijer ◽

E. R. De Kloet ◽

...

Keyword(s):

Glucocorticoid Receptor ◽

Pc12 Cells ◽

Transcriptional Responses ◽

Dynamic Pattern ◽

Neuronal Pc12 Cells

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Effects of a novel pesticide-particle conjugate on viability and reactive oxygen species generation in neuronal (PC12) cells

Drug and Chemical Toxicology ◽

10.3109/01480545.2014.928723 ◽

2014 ◽

Vol 38 (2) ◽

pp. 205-211 ◽

Cited By ~ 1

Author(s):

Aishwarya Sooresh ◽

Christie M. Sayes ◽

Michelle Pine

Keyword(s):

Reactive Oxygen Species ◽

Pc12 Cells ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species ◽

Neuronal Pc12 Cells

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Effect of Neuronal PC12 Cells on the Functional Properties of Intestinal Epithelial Caco-2 Cells

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.67.1312 ◽

2003 ◽

Vol 67 (6) ◽

pp. 1312-1318 ◽

Cited By ~ 8

Author(s):

Hideo SATSU ◽

Tatsuya YOKOYAMA ◽

Nobumasa OGAWA ◽

Yoko FUJIWARA-HATANO ◽

Makoto SHIMIZU

Keyword(s):

Pc12 Cells ◽

Functional Properties ◽

Intestinal Epithelial ◽

Neuronal Pc12 Cells

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Nerve growth factor withdrawal-induced cell death in neuronal PC12 cells resembles that in sympathetic neurons.

The Journal of Cell Biology ◽

10.1083/jcb.119.6.1669 ◽

1992 ◽

Vol 119 (6) ◽

pp. 1669-1680 ◽

Cited By ~ 156

Author(s):

P W Mesner ◽

T R Winters ◽

S H Green

Keyword(s):

Cell Death ◽

Protein Synthesis ◽

Nerve Growth Factor ◽

Growth Factor ◽

Programmed Cell Death ◽

Pc12 Cells ◽

Sympathetic Neurons ◽

Nerve Growth ◽

Neuronal Pc12 Cells ◽

New Protein

Previous studies have shown that in neuronal cells the developmental phenomenon of programmed cell death is an active process, requiring synthesis of both RNA and protein. This presumably reflects a requirement for novel gene products to effect cell death. It is shown here that the death of nerve growth factor-deprived neuronal PC12 cells occurs at the same rate as that of rat sympathetic neurons and, like rat sympathetic neurons, involves new transcription and translation. In nerve growth factor-deprived neuronal PC12 cells, a decline in metabolic activity, assessed by uptake of [3H]2-deoxyglucose, precedes the decline in cell number, assessed by counts of trypan blue-excluding cells. Both declines are prevented by actinomycin D and anisomycin. In contrast, the death of nonneuronal (chromaffin-like) PC12 cells is not inhibited by transcription or translation inhibitors and thus does not require new protein synthesis. DNA fragmentation by internucleosomal cleavage does not appear to be a consistent or significant aspect of cell death in sympathetic neurons, neuronal PC12 cells, or nonneuronal PC12 cells, notwithstanding that the putative nuclease inhibitor aurintricarboxylic acid protects sympathetic neurons, as well as neuronal and nonneuronal PC12 cells, from death induced by trophic factor removal. Both phenotypic classes of PC12 cells respond to aurintricarboxylic acid with similar dose-response characteristics. Our results indicate that programmed cell death in neuronal PC12 cells, but not in nonneuronal PC12 cells, resembles programmed cell death in sympathetic neurons in significant mechanistic aspects: time course, role of new protein synthesis, and lack of a significant degree of DNA fragmentation.

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Extracellular ATP activates NFAT-dependent gene expression in neuronal PC12 cells via P2X receptors

BMC Neuroscience ◽

10.1186/1471-2202-12-90 ◽

2011 ◽

Vol 12 (1) ◽

pp. 90 ◽

Cited By ~ 7

Author(s):

Prabin Prasai ◽

Georgios C Stefos ◽

Walter Becker

Keyword(s):

Gene Expression ◽

Pc12 Cells ◽

P2x Receptors ◽

Extracellular Atp ◽

Neuronal Pc12 Cells

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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/3184019 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Dejene Disasa ◽

Lihong Cheng ◽

Majid Manzoor ◽

Qian Liu ◽

Ying Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Antioxidant Activity ◽

Natural Products ◽

Survival Rate ◽

Pc12 Cells ◽

Neuroprotective Effects ◽

Replicative Lifespan ◽

Antioxidative Stress ◽

Gentiana Rigescens

In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10 μM. Furthermore, amarogentin improved the survival rate of yeast under oxidative stress by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and these enzymes’ gene expression. In addition, this compound did not extend the replicative lifespan of sod1, sod2, uth1, and skn7 mutants with K6001 background. These results suggested that amarogentin exhibited antiaging effect on yeast via increase of SOD2, CAT, GPx gene expression, enzyme activity, and antioxidative stress. Moreover, we evaluated antioxidant activity of this natural products using PC12 cell system, a useful model for studying the nervous system at the cellular level. Amarogentin significantly improved the survival rate of PC12 cells under H2O2-induced oxidative stress and increased the activities of SOD and SOD2, and gene expression of SOD2, CAT, GPx, Nrf2, and Bcl-x1. Meanwhile, the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) of PC12 cells were significantly reduced after treatment of the amarogentin. These results indicated that antioxidative stress play an important role for antiaging and neuroprotection of amarogentin. Interestingly, amarogentin exhibited neuritogenic activity in PC12 cells. Therefore, the natural products, amarogentin from G. rigescens with antioxidant activity could be a good candidate molecule to develop drug for treating neurodegenerative diseases.

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Preemptive Analgesic and Antioxidative Effect of Curcumin for Experimental Migraine

BioMed Research International ◽

10.1155/2017/4754701 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

Adriana E. Bulboacă ◽

Sorana D. Bolboacă ◽

Ioana C. Stănescu ◽

Carmen A. Sfrângeu ◽

Angelo C. Bulboacă

Keyword(s):

Formalin Test ◽

Saline Solution ◽

Oxidative Status ◽

Control Group ◽

Antioxidative Effect ◽

Stress Effects ◽

Oxidative Stress Parameters ◽

Total Oxidative Status ◽

Antioxidative Stress ◽

Stress Parameters

Objective. Our study aimed to investigate the analgesic and antioxidative stress effects of Curcumin (CC) in experimental migraine induced by Nitroglycerin (NTG) on rats, compared with Indomethacin (ID) and Propranolol (PP) treatments. Material and Methods. Five groups of 10 rats treated i.p. were investigated: control group (healthy rats) injected with saline solution (0.9%), NTG-control group injected with NTG (1 mg/100 gbw, bw = body weight), and three groups with pretreatment applied 30 min previous to the formalin test (NTG + CC group: Curcumin (10 mg/100 gbw), NTG + PP group: Propranolol (100 μg/100 gbw), and NTG + ID group: Indomethacin (0.5 mg/100 gbw)). Formalin test was performed and number of flinches and shakes were counted. Several oxidative stress parameters were also assessed. Results. The smallest values of malondialdehyde (MDA), nitric oxide (NOx), and total oxidative status (TOS) were observed on NTG + CC with significant differences as compared with the control group (p<0.0001). The group pretreated with Curcumin proved significantly smaller number of flinches and shakes compared with both NTG + PP and NTG + ID. Conclusion. Our study demonstrates a superior activity of Curcumin not only versus control, but also versus Propranolol and Indomethacin.

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