scholarly journals Factors associated to the positive cerebrospinal fuid culture in the tuberculous meningitis

2007 ◽  
Vol 65 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Marzia Puccioni-Sohler ◽  
Carlos Otávio Brandão
Keyword(s):  
Tuberculous Meningitis ◽  
Neurological Complication ◽  
Central Nervous System Involvement ◽  
Positive Culture ◽  
Acute Stage ◽  
Chain Reaction ◽  
High Protein Content ◽  
Factors Associated ◽  
Polymerase Chain ◽  
Csf Examination

Central nervous system involvement is the most common neurological complication in the course of tuberculosis. The lack of rapid and sensitive tests delays the early diagnosis. Here, we retrospectively reviewed the cerebrospinal fluid (CSF) examination of 30 patients with tuberculous meningitis confirmed by bacteriological tests (culture and/or polymerase chain reaction). The purpose of the present study was to determine the CSF parameters associated to the positive CSF culture for Mycobacterium tuberculosis in tuberculous meningitis. We found higher frequency of positive CSF culture in patients infected with HIV as well in patients with high number of neutrophils and high protein content (characteristic in the early or acute-stage patients), which suggests that the positive culture found in these patients may be associated with the presence of high bacillary load in CSF occurring in these stages.

Early diagnosis of tuberculous meningitis by polymerase chain reaction

Neurology ◽  
1995 ◽  
Vol 45 (12) ◽  
pp. 2228-2232 ◽  
Author(s):  
L. F.F. Kox ◽  
S. I. Kuijper ◽  
A. H.J. Kolk
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Diagnosis ◽  
Tuberculous Meningitis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals TRC4 Gene Based PCR Assay in Diagnosis of Tuberculous Meningitis

2017 ◽  
Vol 8 (2) ◽  
pp. 19-22
Author(s):  
Abu Naser Ibne Sattar ◽  
Sanjida Khondakar Setu ◽  
Ahmed Abu Saleh ◽  
Sharmeen Ahmed
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Manifestation ◽  
Tuberculous Meningitis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Early Stages ◽  
Lowenstein Jensen ◽  
Polymerase Chain ◽  
Tb Pcr ◽  
Is6110 Element

The prevalence of Tuberculous meningitis remains largely underestimated due to nonspecific clinical manifestation at early stages. A total of 20 CSF samples were studied from clinically suspected cases of tuberculous meningitis. All the samples were processed for ZN staining, TB culture on LJ media and TB-PCR using IS6110 and TRC4 primers by standard protocols. Of the total 20 CSF samples, 12 cases were diagnosed as TB meningitis. Most of the tuberculous meningitis cases were found positive by PCR using TRC4 primers (83.33%) followed by IS6110 primer (66.67%) and culture on L-J media(8.33%). None were found positive by ZN staining. TB-PCR usingTRC4 primer showed higher positivity than using IS6110 in detecting tuberculous meningitis, since some strains of MTB may lack the IS6110 element in their genome. PCR assay using TRC4 primer is superior in diagnosing tuberculous meningitis. This study was aimed to evaluate the diagnostic potentials of CSF polymerase chain reaction (PCR) by using TRC4 and IS6110 primers. Further the results were also compared with culture on Lowenstein-Jensen (LJ) media and AFB smear by Zeihl-Nelson (ZN) staining.Bangladesh J Med Microbiol 2014; 08 (02): 19-22

scholarly journals Factors Associated with HIV prevalence among HIV-exposed Infants with HIV DNA Polymerase Chain Reaction Tests in Malawi: 2013-2020

2021 ◽  
Author(s):  
Wingston Ng'ambi ◽  
Janne Estill ◽  
Fatma Aziza Merzouki ◽  
Erol Orel ◽  
Tiwonge Chimpandule ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Temporal Trends ◽  
Chain Reaction ◽  
Factors Associated ◽  
Hiv Dna ◽  
Polymerase Chain ◽  
Hiv Exposed ◽  
Spatial And Temporal Trends ◽  
Dna Pcr ◽  
Test Result

Background: Despite the high availability of individual-level data of infants accessing HIV DNA polymerase chain reaction (DNA-PCR) testing service, there has been little in-depth analysis of such data. Therefore, we describe spatial and temporal trends in risk of HIV infection among Malawi HIV-exposed infants (HEI) with DNA-PCR HIV test result from 2013 to 2020. Methods: This is an implementation study using routinely collected patient-level HIV DNA-PCR test result data extracted from the national Laboratory Management Information System database managed by the Department of HIV/AIDS between 1 January 2013 and 30 June 2020. We calculated frequencies, proportions and odds ratios (OR) with their associated 95% confidence intervals (95%CI). We performed a random-effects logistic regression to determine the risk factors associated with HIV infection in infants, controlling for the spatial autocorrelation between districts and adjusting for other variables. Results: We evaluated 255,229 HEI across 750 facilities in 28 districts. The overall risk of HIV infection among all tested HEI between 2013 and 2020 was 7.2% (95%CI: 7.1-7.3). We observed a decreasing trend in the proportion of HEI that tested HIV positive from 7.0% (95%CI: 6.6-7.4) in 2013 to 5.7% (95%CI: 5.4-5.9) in 2015 followed by an increase to 9.9% (95%CI: 9.6-10.2) in 2017 and then a decreasing trend to 4.2% (95%CI: 3.7-4.6) in 2020. The risk of HIV infection increased by age of the HEI. There was spatial heterogeneity of HIV prevalence between districts of Malawi. Conclusion: We summarised spatial and temporal trends of risk of HIV infection amongst HEI in Malawi between 2013 and 2020. There is need for further strengthening of EID program to ensure that all the HEI are enrolled in care by eight weeks of age in order to further reduce mother-to-child transmission of HIV.

scholarly journals Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon

2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Andréia Ferreira da Silva ◽  
Ágatha Rossanni Alves Damasceno ◽  
Welton Seabra Prado ◽  
Rossela Damasceno Caldeira ◽  
Francisco Dantas Sampaio-Junior ◽  
...  
Keyword(s):  
Canine Visceral Leishmaniasis ◽  
Risk Map ◽  
Intact Skin ◽  
Chain Reaction ◽  
Blood Samples ◽  
Factors Associated ◽  
Forested Areas ◽  
Polymerase Chain ◽  
Collection Sites ◽  
Skin Samples

ABSTRACT: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and Itaboca in the municipality of Inhangapi, Pará, Brazil. Whole blood and intact skin samples were collected from 143 dogs, and a questionnaire was applied. L. infantum DNA was detected by polymerase chain reaction (PCR) using primers RV1 and RV2. Collection sites were georeferenced to obtain a spatial distribution of the residences visited and infected dogs. L. infantum DNA was detected in 8.4% (12/143) of the skin samples and in 1.4% (2/143) of the blood samples. On the risk map, three clusters were observed in Itaboca and one was observed in Menino Jesus de Petimandeua. We observed that most of the inhabitants in these maroon communities live close to forested areas and do not use protection against insect vectors. The presence of canine reservoirs of L. infantum associated to environment characteristics (preserved forests and deforested areas) and habits of dog owners (living near forested areas and not using any protection against insects) may favor the transmission of L. infantum in the studied areas.

The Potential Contribution of the Polymerase Chain Reaction to the Diagnosis of Tuberculous Meningitis

1996 ◽  
Vol 53 (8) ◽  
pp. 771-776 ◽  
Author(s):  
L. N. Nguyen ◽  
L. F. F. Kox ◽  
L. D. Pham ◽  
S. Kuijper ◽  
A. H. J. Kolk
Keyword(s):  
Polymerase Chain Reaction ◽  
Tuberculous Meningitis ◽  
Potential Contribution ◽  
Chain Reaction ◽  
Polymerase Chain
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