scholarly journals Occurence of Giardia lamblia in children of municipal day-care centers from Botucatu, São Paulo State, Brazil

1995 ◽  
Vol 37 (6) ◽  
pp. 501-506 ◽  
Author(s):  
Semíramis Guimarães ◽  
Maria Inês L. Sogayar
Keyword(s):  
Giardia Lamblia ◽  
Day Care ◽  
Stool Specimen ◽  
School Age Children ◽  
Blastocystis Hominis ◽  
Day Care Centers ◽  
Staff Members ◽  
Parasitological Survey ◽  
Stool Specimens ◽  
The City

Considering that the number of day-care centers for pre-school-age children has expanded rapidly in developing countries, and that these institutions presenting conditions that facilitate the transmission of many enteric agents, a parasitological survey was carried out in three municipal day-cares from Botucatu: two in the urban area (one in downtown area and the other one in the city periphery area) and the third in the rural area. Three separate stool specimens were collected from 147 children ranging from 0 to 72 months old and 20 staff members. Each stool specimen was processed by Lutz and zinc sulfate flotation methods. The frequency of giardiasis observed among children of downtown, periphery and rural day-cares was 69.6%, 52.7% and 69.6%, respectively. Only one employee was positive for G. lamblia. The examination of three stool specimens increased the positivity for G. lamblia: from the ninety three final positive examinations, 24 (25.5%) and 8 (8.5%) were positives only after examination of the second and third samples, respectively. Others intestinal organisms like Ascaris lumbricoides (20.4%), Trichuris trichiura (19.0%). Hymenolepis nana (8.8%), Entamoeba coli (22.4%) and Blastocystis hominis (32.0%) were frequently found in the children. There was no significant association among localization of the day-cares, sex of the children and the levels of G. lamblia infection. According to the age, G. lamblia was found mainly in children between 12 to 47 months old.

Stool Survey of Asymptomatic Diapered Children in Day Care

PEDIATRICS ◽  
1994 ◽  
Vol 94 (6) ◽  
pp. 1026-1027
Author(s):  
Michele M. Ginsberg ◽  
Kathleen Keenan ◽  
Muriel Thompson ◽  
Bronwen Anders
Keyword(s):  
Giardia Lamblia ◽  
Day Care ◽  
Asymptomatic Infection ◽  
Day Care Centers ◽  
Enteric Diseases ◽  
Department Of Health ◽  
Asymptomatic Children ◽  
Stool Samples ◽  
Stool Specimens ◽  
Stool Cultures

A survey of diaper-wearing children in community-day-care centers was conducted by the San Diego Department of Health Services during the period February-April 1986 to determine the presence of enteric pathogens in asymptomatic children. Written consent to collect stool samples from diapers was obtained from parents of children at four preschools. No reportable enteric diseases or outbreaks had occurred at the schools in the preceding year. For each child, the preschool teachers maintained a record of symptoms (eg, diarrhea, fever) during the week of stool collection. Stool specimens were collected from 63 diapered children. The samples were examined for parasites and cultured for bacteria and viruses. Giardia lamblia was identified in 18% to 32% of stool samples collected from children at three of the four centers. Prevalence of Giardia varied with the age of the child (0% age <12 months to 33% age <23 months). Microscopic examinations for Cryptosporidium and pathogenic amoeba were negative. Stool cultures were negative for Salmonella, Shigella, and Campylobacter. The prevalence of adenoviruses identified by viral cultures ranged from 32% to 80% at four sites. Echovirus was isolated from 36% of samples at one center. All stools were negative for rotavirus. Giardia was identified in the stools of 22% of children in day care in the absence of diarrhea or of concurrent illness. These findings indicate that asymptomatic infection with Giardia occurs commonly among children attending day-care centers. The lack of any documented outbreak or symptoms of enteric disease among attendees suggests that these asymptomatic children with Giardia need not be excluded from day care.

scholarly journals Detection of anti-Giardia lamblia serum antibody among children of day care centers

2002 ◽  
Vol 36 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Semíramis Guimarães ◽  
Maria Inês L Sogayar
Keyword(s):  
Giardia Lamblia ◽  
Day Care ◽  
Serum Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Children ◽  
Serum Antibodies ◽  
Serological Tests ◽  
Day Care Centers ◽  
Blood Samples ◽  
Stool Specimens

OBJETIVES: To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas. METHODS: Three separate stool specimens and filter paper blood samples were collected from 147 children ranging from 0 to 6 years old. Each stool sample was processed using spontaneous sedimentation and zinc sulfate flotation methods. Blood samples were tested by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) for Giardia IgG. RESULTS AND CONCLUSIONS: Of 147 individuals tested, 93 (63.3%) showed Giardia cysts in their feces. Using IIF and ELISA, serum antibodies were detected in 93 (63.3%) and 100 (68%) samples , respectively. Sensitivity of IIF and ELISA was 82% and 72%, respectively. However, ELISA revealed to be less specific (39%) than IIF (70%). IIF also showed a higher concordance with microscopic examination than ELISA.

scholarly journals Longitudinal testing for SARS-CoV-2 RNA in day care centers in Hesse, Germany, during increased local incidence and with VOC Alpha as dominant variant: Results of the SAFE KiDS 2 and SAFE KiDS 3 study

2021 ◽  
Author(s):  
Barbara Schenk ◽  
Sebastian Hoehl ◽  
Olga Rudych ◽  
Emilie Kreutzer ◽  
Dominic Menger ◽  
...  
Keyword(s):  
Day Care ◽  
Care Setting ◽  
Local Community ◽  
Infectious Period ◽  
Day Care Centers ◽  
Staff Members ◽  
Viral Shedding ◽  
Daycare Centers ◽  
Alpha Variant ◽  
The Impact

In the summer of 2020, we investigated the rate of inapparent shedding of SARS-CoV-2 in a representative sample of day care centers from Hesse, Germany, and found a low positivity rate during a period of low local community spread. To investigate the influence of a high local incidence setting, we conducted the SAFE KiDS 2 study. 577 children and 334 staff members of 47 daycare centers were tested for respiratory and gastrointestinal shedding of SARS-CoV-2, and three infections with SARS-CoV-2 in the infectious period were detected. We conclude that viral shedding occurred infrequently while the original "wild-type" variant was dominant. The more transmissible SARS-CoV-2 variant Alpha (B.1.1.7) became the dominant strain after the SAFE KiDS 2 was concluded. The SAFE KiDS 3 study investigated the impact of the Alpha variant of SARS-Co-2 on inapparent viral shedding in the day care setting. In this study, 756 children and 226 staff members from 46 day care centers provided self-collected saliva swabs, the so-called "Lollipop" swabs, which were tested by RT-PCR. In the four-week study period, none of the participants tested positive for SARS-CoV-2 RNA, demonstrating that inapparent shedding of SARS-CoV-2 in the day care setting was also rare during the dominance of the Alpha variant. The influence of the variant of concern Delta on day care centers has yet to be examined.

Endemic Giardiasis and Day Care

PEDIATRICS ◽  
1983 ◽  
Vol 72 (2) ◽  
pp. 154-158
Author(s):  
David P. Sealy ◽  
Stanley H. Schuman
Keyword(s):  
South Carolina ◽  
Preschool Children ◽  
Day Care ◽  
Care Setting ◽  
First Grade ◽  
Rural County ◽  
White Children ◽  
Day Care Centers ◽  
Naturally Occurring ◽  
Stool Specimens

Five surveys of 1,731 children for stool ova and parasites (1971 to 1981) in a rural county provide a unique perspective on naturally occurring, nonepidemic giardiasis. Currently white children in day care centers in Hampton County, South Carolina, experience attack rates of 26%. They enter the first grade with at least six times as much infection as those who do not attend day care. A trend toward more giardiasis linked to working mothers and day care is evident among white preschool children. This has not yet occurred among black preschool-aged children. These and other epidemiologic data indicate that as few as 100 children can maintain endemic levels of infection in a county of 18,000 residents. Person-to-person transmission in the day care setting is sufficient to explain this county's rising rate of stool positivity of infection (8% of all stool specimens submitted to the state laboratory).

scholarly journals TRIMETHOPRIM RESISTANT (TMPR) E. COLI IN STOOL SPECIMENS FROM CHILDREN IN DAY CARE CENTERS (DCC)

1987 ◽  
Vol 21 (4) ◽  
pp. 332A-332A
Author(s):  
Randall R Reves ◽  
Barbara E Murray ◽  
Mina Fong ◽  
Larry K Pickering
Keyword(s):  
Day Care ◽  
Day Care Centers ◽  
E Coli ◽  
Stool Specimens

scholarly journals Controlled trial of Giardia lamblia: control strategies in day care centers.

1991 ◽  
Vol 81 (8) ◽  
pp. 1001-1006 ◽  
Author(s):  
A V Bartlett ◽  
S J Englender ◽  
B A Jarvis ◽  
L Ludwig ◽  
J F Carlson ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Day Care ◽  
Control Strategies ◽  
Controlled Trial ◽  
Day Care Centers

Agitation Among Elderly Persons at Adult Day-Care Centers: The Experiences of Relatives and Staff Members

1995 ◽  
Vol 7 (3) ◽  
pp. 447-458 ◽  
Author(s):  
Jiska Cohen-Mansfield ◽  
Perla Werner ◽  
Valerie Watson ◽  
Sonia Pasis
Keyword(s):  
Nursing Home ◽  
Day Care ◽  
Adult Day Care ◽  
Elderly Persons ◽  
Factor Solution ◽  
Nursing Home Population ◽  
Day Care Centers ◽  
Staff Members ◽  
Verbal Behaviors ◽  
Three Factor

Two hundred participants (mean age = 80 years) from five senior day-care centers were included in a study of agitation. Staff members at the centers and participants' relatives rated the frequency with which participants displayed agitated behaviors, via an expanded version of the Cohen-Mansfield Agitation Inventory. The most frequent behaviors noted were general restlessness, repetitious sentences, verbal interruptions, and pacing. A three-factor solution for staff members' ratings included (a) physically nonaggressive behaviors, including general restlessness and pacing; (b) verbally agitated behaviors, including complaining and constant requests for attention; and (c) aggressive verbal behaviors, including cursing and temper outbursts. A three-factor solution for relatives' ratings included (a) physically nonaggressive behaviors, including general restlessness and pacing; (b) verbally agitated behaviors, including constant requests for attention and related interruptions; and (c) aggressive behaviors, including cursing, grabbing, kicking, and pushing. The syndromes of both models showed similarity to the factors found in a nursing home population, although differences were also apparent.

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