scholarly journals Caracterização da agressividade de isolados de Colletotrichum de maracujá amarelo com marcadores bioquímico, fisiológico e molecular

2007 ◽  
Vol 32 (4) ◽  
pp. 318-328 ◽  
Author(s):  
Luiz Carlos C. Almeida ◽  
Rildo S.B. Coêlho
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Glomerella Cingulata ◽  
Colletotrichum Spp

A antracnose é a doença pós-colheita mais importante do maracujá amarelo, cujo agente etiológico, no Brasil, foi identificado como Colletotrichum gloeosporioides. Visando caracterizar o patógeno, foram obtidos 33 isolados de três regiões produtoras do estado de Pernambuco. Critérios morfológicos como cor de colônia, forma e dimensão de conídios, a produção de peritécio e o uso de primers específicos para C. acutatum, C. gloeosporioides e "Colletotrichum de Passiflora" permitiram identificar Glomerella cingulata patótipo 1, G. cingulata patótipo 2, Colletotrichum sp. de Passiflora e Colletotrichum sp. de maracujá amarelo. Inoculações em maracujá amarelo possibilitaram separar os isolados em dois grupos, um de agressividade alta (GA-1) e outro de agressividade baixa (GA-2). Os marcadores bioquímicos como atividade enzimática amilolítica, celulolítica, lipolítica e proteolítica assim como o marcador fisiológico crescimento micelial não separaram os isolados pela agressividade. O padrão de marcas geradas pela amplificação dos DNAs dos isolados usando primers RAPD evidenciou que os isolados do GA-1 variaram menos geneticamente entre si do que os isolados do GA-2, demonstrando que os do GA-1 evoluíram mais recentemente. A amplificação do DNA dos isolados com o primer OPA-9 gerou um marcador que possibilitou caracterizar 85,7% dos isolados do GA-1 e também alguns isolados do GA-2 com agressividade próxima às dos isolados do GA-1, e por isto o primer OPA-9 pode ser usado para caracterizar isolados de Colletotrichum spp. de alta agressividade em programa de resistência genética.

Differentiation of Isolates of Glomerella cingulata and Colletotrichum spp. Associated with Glomerella Leaf Spot and Bitter Rot of Apples Using Growth Rate, Response to Temperature, and Benomyl Sensitivity

2005 ◽  
Vol 6 (1) ◽  
pp. 6 ◽  
Author(s):  
Eugenia González ◽  
Turner B. Sutton
Keyword(s):  
Growth Rate ◽  
Leaf Spot ◽  
Molecular Diversity ◽  
Colletotrichum Gloeosporioides ◽  
Glomerella Cingulata ◽  
Optimum Growth Temperature ◽  
Bitter Rot ◽  
Colletotrichum Spp ◽  
Glomerella Leaf Spot ◽  
Response To Temperature

Cultural characteristics were investigated as a way to distinguish isolates of Glomerella cingulata and Colletotrichum spp. associated with Glomerella leaf spot and bitter rot of apples from those that cause only bitter rot. The growth rate, response to temperature, and benomyl sensitivity of 27 isolates of Glomerella cingulata, 12 isolates of Colletotrichum gloeosporioides, and 7 isolates of C. acutatum, collected from apple orchards located in the U.S. and Brazil and previously characterized based on morphology, vegetative compatibility, and mitochondrial DNA (mtDNA) haplotypes, were determined. These isolates represent the genetic and molecular diversity within isolates of C. gloeosporioides, C. acutatum, and G. cingulata from apples found in a previous study. Slower growth, lower optimum growth temperature, and less sensitivity to benomyl distinguished isolates of C. acutatum from isolates of G. cingulata and C. gloeosporioides. However, growth rate and benomyl sensitivity were not useful for distinguishing between G. cingulata and C. gloeosporioides or differentiating isolates of G. cingulata that cause leaf spot and bitter rot from those that only cause bitter rot. Accepted for publication 17 May 2005. Published 19 July 2005.

scholarly journals Identificación y caracterización de Colletotrichum spp. causante de antracnosis en aguacate de Nayarit, México

2017 ◽  
pp. 3953 ◽  
Author(s):  
Eddy Trinidad-Ángel ◽  
Felipe De Jesús Ascencio-Valle ◽  
Osé Armando Ulloa ◽  
Osé Carmen Ramírez-Ramírez ◽  
Juan Arturo Ragazzo-Sánchez ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Spp

La antracnosis, ocasionada por Colletotrichum spp., es una de las principales enfermedades del aguacate que afecta la calidad del fruto y merma su producción. En los últimos años se han reportado dificultades para la prevención y erradicación de esta enfermedad. Por ello, el objetivo del presente trabajo fue identificar y caracterizar cepas de Colletotrichum spp. como agente causal de antracnosis en el aguacate ‘Hass’., mediante estudios morfológicos, patogénicos y moleculares, a partir de aislamientos provenientes de las principales zonas productoras de aguacate del estado de Nayarit, México. Se recolectaron frutos con síntomas típicos de la enfermedad, colectados en cuatro ejidos pertenecientes a los municipios de Xalisco y Tepic, Nayarit, México. Se obtuvo una colección de 20 aislamientos, de los cuales 13 son de C. gloeosporioides, una C. hymenocallidis, cinco C. siamense y una de C. tropicale, siendo la más patógena el Colletotrichum gloeosporioides. Es importante destacar que este es el primer reporte de C. hymenocallidis y C. siamense en aguacate.    

scholarly journals Identification and Characterization of Benomyl-Resistant and -Sensitive Populations of Colletotrichum gloeosporioides from Statice (Limonium spp.)

2006 ◽  
Vol 96 (5) ◽  
pp. 542-548 ◽  
Author(s):  
Marcel Maymon ◽  
Aida Zveibil ◽  
Shimon Pivonia ◽  
Dror Minz ◽  
Stanley Freeman
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Sequence Analyses ◽  
Specific Primers ◽  
Tubulin Genes ◽  
Colletotrichum Spp ◽  
Polymerase Chain ◽  
Species Specific ◽  
Identification And Characterization ◽  
Propagation Material

Sixty-four isolates of Colletotrichum gloeosporioides were isolated from infected Limonium spp. cultivated in 12 different locations in Israel. All isolates were identified as belonging to the C. gloeosporioides complex by species-specific primers. Of these isolates, 46 were resistant to benomyl at 10 μg/ml and 18 were sensitive to this concentration of fungicide. Based on arbitrarily primed polymerase chain reaction of all isolates and internal transcribed spacer-1 sequence analyses of 12 selected isolates, the benomyl-resistant and -sensitive populations belong to two distinct genotypes. Sequence analyses of the β-tubulin genes, TUB1 and TUB2, of five sensitive and five resistant representative isolates of C. gloeosporioides from Limonium spp. revealed that the benomyl-resistant isolates had an alanine substitute instead of a glutamic acid at position 198 in TUB2. All data suggest that the resistant and sensitive genotypes are two independent and separate populations. Because all Limonium plant propagation material is imported from various geographic regions worldwide, and benomyl is not applied to this crop or for the control of Colletotrichum spp. in Israel, it is presumed that plants are bearing quiescent infections from the points of origin prior to arrival.

scholarly journals Genetic Diversity of Colletotrichum spp. Causing Strawberry Anthracnose in Zhejiang, China

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1351-1357 ◽  
Author(s):  
X. Y. Chen ◽  
D. J. Dai ◽  
S. F. Zhao ◽  
Y. Shen ◽  
H. D. Wang ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Dominant Species ◽  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Zhejiang Province ◽  
Control Measures ◽  
Chitin Synthase ◽  
Colletotrichum Gloeosporioides Complex ◽  
Strawberry Anthracnose ◽  
Colletotrichum Spp

Anthracnose is a serious fungal disease that primarily infects strawberry roots and stolons during development. Here, 91 isolates from different areas of Zhejiang province, China, were collected. Morphological characteristics were analyzed, and a phylogenetic analysis based on multiple genes (actin, internal transcribed spacer, calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase) was performed. We found that all of the Colletotrichum species causing strawberry anthracnose belonged to the Colletotrichum gloeosporioides complex. Among them, we identified 48 isolates of C. fructicola, 21 isolates of C. siamense, 13 isolates of C. gloeosporioides, and 9 isolates of C. aenigma. C. siamense was distributed in the central and eastern regions of Zhejiang province (Hangzhou, Jinhua, Shaoxing, Ningbo, and Taizhou). This is the first report of C. siamense causing strawberry anthracnose in Zhejiang province. C. fructicola was the most dominant species causing strawberry anthracnose in Zhejiang province. We identified the four species causing strawberry anthracnose in Zhejiang province, which will improve our understanding of the strawberry anthracnose epidemic and will benefit the development of future control measures.

scholarly journals Colletotrichum gloeosporioides sensu stricto Is a Pathogen of Leaf Anthracnose on Evergreen Spindle Tree (Euonymus japonicus)

Plant Disease ◽  
2016 ◽  
Vol 100 (4) ◽  
pp. 672-678 ◽  
Author(s):  
Lin Huang ◽  
Qiu-Cheng Li ◽  
Ya Zhang ◽  
De-Wei Li ◽  
Jian-Ren Ye
Keyword(s):  
Fungal Pathogen ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungi ◽  
Sensu Stricto ◽  
Economic Importance ◽  
Fungal Isolate ◽  
Important Group ◽  
Wide Range ◽  
Colletotrichum Spp ◽  
Euonymus Japonicus

The genus Colletotrichum is considered the eighth most important group of plant-pathogenic fungi in the world due to its scientific and economic importance. Colletotrichum spp. cause anthracnose disease in a wide range of economically important plants. Euonymus japonicus Thunb. (Celastraceae) is a broad-leaved evergreen tree that is widely planted in the parks and landscapes of China. An anthracnose occurs on E. japonicus in China but there has been a disagreement on the identity of the fungal pathogen. In this study, the fungal isolate HYCG2-3 was determined by Koch’s postulates to be a pathogen on E. japonicus. Based on the morphological and molecular methods, isolate HYCG2-3 was identified as Colletotrichum gloeosporioides sensu stricto within the C. gloeosporioides species complex.

Susceptibility of Fuerte avocado fruit at various stages of growth, to infection by anthracnose and stem end rot fungi

1978 ◽  
Vol 18 (90) ◽  
pp. 158 ◽  
Author(s):  
RA Peterson
Keyword(s):  
Field Experiments ◽  
Colletotrichum Gloeosporioides ◽  
Natural Infection ◽  
Persea Americana ◽  
Glomerella Cingulata ◽  
Stages Of Growth ◽  
Small Fruit ◽  
Avocado Fruit ◽  
Time Of Infection

In a series of field experiments, Fuerte avocado fruit (Persea americana) were susceptible to infection by Glomerella cingulata var. minor (Colletotrichum gloeosporioides var, minor) and Dothiorella aromatics, from set until harvest. Natural infection was detected from December to May and field inoculations demonstrated that small fruit in October were also susceptible. The time of infection varied with the season and was related to rainfall incidence. The significance of this finding is discussed.

Anthracnose and Berry Disease of Coffee in Puerto Rico

1969 ◽  
Vol 69 (1) ◽  
pp. 107-117
Author(s):  
J. S. Mignucci ◽  
P. R. Hepperly ◽  
J. Ballester ◽  
C. Rodríguez-Santiago
Keyword(s):  
Puerto Rico ◽  
Colletotrichum Gloeosporioides ◽  
High Rate ◽  
Glomerella Cingulata ◽  
Collar Rot ◽  
In Vitro Inoculation ◽  
Cercospora Coffeicola ◽  
Necrotic Spots ◽  
Normal Fertilization

A survey revealed that Anthracnosis (Glomerella cingulata asex. Colletotrichum gloeosporioides) was the principal aboveground disease of field coffee in Puerto Rico. Isolates of C. gloeosporioides from both diseased soybeans and coffee caused typical branch necrosis in coffee after in vitro inoculation. Noninoculated checks showed no symptoms of branch necrosis or dieback. Necrotic spots on coffee berries collected from the field were associated with the coffee anthracnose fungus (C. gloeosporioides), the eye spot fungus (Cercospora coffeicola) and the scaly bark or collar rot fungus (Fusarium stilboides). Typical lesions were dark brown, slightly depressed and usually contained all three fungi. Fascicles of C. coffeicola conidiophores formed a ring inside the lesion near its periphery. Acervuli of C. gloeosporioides and the sporodochia of F. stilboides were mixed in the center of the lesions. Monthly fungicide sprays (benomyl plus captafol) and double normal fertilization (454 g 10-5-15 with micronutrients/tree, every 3 months) partially controlled berry spotting. Double normal fertilizer applications alone appeared to reduce the number of diseased berries by approximately 41%, but fungicide sprays gave 57% control. Combining high rate of fertilization and fungicide applications resulted in a reduction of approximately 85% of diseased berries.

scholarly journals PRODUTOS ALTERNATIVOS NO CONTROLE DE Colletotrichum spp. ISOLADOS DE MANGA E BANANA

2018 ◽  
Vol 5 (3) ◽  
pp. 104-112
Author(s):  
Amanda Cristina De Araújo ◽  
Eliane Divina Toledo ◽  
William Rosa de Oliveira Soares
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Spp ◽  
Colletotrichum Musae

A antracnose causada pelo fungo Colletotrichum spp. ocorre tanto na cultura da manga quanto na cultura da banana, em campo e em pós colheita. A busca pela incorporação de uma nova consciência ecológica vem trazendo conceitos de sustentabilidade para o meio agrícola. Nesse sentido, objetivou-se com este trabalho avaliar o efeito de produtos alternativos em diferentes concentrações sobre o desenvolvimento de Colletotrichum spp. in vitro. Os isolados foram obtidos de frutos com sintomas de antracnose. A atividade antifúngica das concentrações dos tratamentos foi avaliada através da medição em centímetros do crescimento micelial do patógeno. Utilizaram-se cinco concentrações (1, 2, 4, 8, e 16%) dos tratamentos  com essenciais de eucalipto, copaíba , coco, cravo-da-Índia   e alecrim  dos extratos de citronela canela e cravo-da-Índia , do hidrolato de cravo-da-Índia  e do composto majoritário  do óleo de cravo-da-Índia Eugenol  em meio de cultura BDA. O ensaio foi conduzido em dois experimentos com delineamento inteiramente casualizado, em esquema fatorial 10x5 e 7x5 para Colletotrichum musae e Colletotrichum gloeosporioides respectivamente em cada produto utilizado/concentração foram utilizadas quatro repetições. os tratamentos eugenol e óleo de alecrim apresentaram os melhores resultados quanto a inibição do crescimento do fungo em todas concentrações utilizadas. Destaca-se o tratamento com óleo de eucalipto e de cravo que também inibiram totalmente o crescimento de C. musae a partir da concentração 2%. Na avaliação do crescimento de C. gloeosporioides, dentre os sete tratamentos avaliados, apenas o eugenol apresentou potencial total de inibição de crescimento independente da concentração utilizada, seguido do óleo de copaíba que apresentou redução do crescimento em todas as concentrações quando comparado aos demais tratamentos.

scholarly journals Caracterização morfo-fisiológica e patogenicidade de Colletotrichum gloeosporioides da pupunheira

2006 ◽  
Vol 32 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Rudimar Mafacioli ◽  
Dauri José Tessmann ◽  
Álvaro Figueiredo dos Santos ◽  
João Batista Vida
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Sao Paulo ◽  
São Paulo ◽  
Bactris Gasipaes ◽  
Glomerella Cingulata ◽  
Espírito Santo ◽  
Espirito Santo

Isolados de Colletotorichum sp. de folhas de pupunheira (Bactris gasipaes) com sintomas de antracnose foram comparados fenotipicamente, visando a sua caracterização e identificação. Foram analisados 17 isolados, oriundos dos Estados do Acre, Rondônia, Espírito Santo, São Paulo e Paraná. Caracterizaram-se os sintomas da doença e os isolados foram comparados em relação à forma e tamanho de conídios e apressórios, e quanto a coloração das colônias, crescimento micelial e esporulação em meio de cultura. A patogenicidade dos isolados foi confirmada em folhas de pupunheira destacadas. Todos os isolados foram identificados como Colletotrichum gloeosporioides e a fase meiospórica in vitro (Glomerella cingulata) ocorreu apenas em um isolado procedente de Linhares, Espírito Santo.

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