scholarly journals Root colonization and interaction among growth promoting rhizobacteria isolates and eucalypts species

2009 ◽  
Vol 33 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Reginaldo Gonçalves Mafia ◽  
Acelino Couto Alfenas ◽  
Eraclides Maria Ferreira ◽  
Daniel Henrique Breda Binoti ◽  
Gizella Machado Ventura Mafia ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stenotrophomonas Maltophilia ◽  
Root Colonization ◽  
Growth Promotion ◽  
Eucalyptus Species ◽  
Growth Promoter ◽  
Pseudomonas Sp ◽  
Potential Growth ◽  
Growth Promoting

This work aimed to evaluate root colonization and interaction among isolates of rhizobacteria and eucalypt species. The method used to evaluate "in vitro" root colonization was able to indicate if the effect was benefic or deleterious allowing to pre-select isolates as potential growth promoter. There was interaction among isolates of rhizobacteria and Eucalyptus species for seed germinating and seedling growth. MF2 (Pseudomonas sp.) was the best rhizobacteria isolate for growth promotion of E. cloeziana e E. grandis. S1 (Bacillus subtilis) was the most effective for E. globulus, and Ca (Pseudomonas fulva), MF2 (Pseudomonas sp.), CIIb (Stenotrophomonas maltophilia) and S2 (B. subtilis) were the most promising isolates for the E. urophylla.

Enhancement of verticillium wilt resistance in tomato transplants by in vitro co-culture of seedlings with a plant growth promoting rhizobacterium (Pseudomonas sp. strain PsJN)

1998 ◽  
Vol 44 (6) ◽  
pp. 528-536 ◽  
Author(s):  
V K Sharma ◽  
J Nowak
Keyword(s):  
Plant Growth ◽  
Verticillium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Pseudomonas Sp ◽  
Plant Growth Promoting ◽  
Growth Promoting

The potential utilization of a plant growth promoting rhizobacterium, Pseudomonas sp. strain PsJN, to enhance the resistance of tomato transplants to verticillium wilt was investigated. Plant growth and disease development were tested on the disease-susceptible cultivar Bonny Best after Verticillium dahliae infection of tissue culture plantlets bacterized in vitro (by co-culturing with the bacterium) and seedlings bacterized in vivo (after 3 weeks growth in the greenhouse). Significant differences in both disease suppression and plant growth were obtained between in vitro bacterized and nonbacterized (control) plants. The degree of protection afforded by in vitro bacterization depended on the inoculum density of V. dahliae; the best and worst protection occurred at the lowest (103 conidia ·mL-1) and highest (106 conidia ·mL-1) levels, respectively. In contrast, the in vivo bacterized tomatoes did not show plant growth promotion when compared to the nonbacterized control plants. When challenged with Verticillium, significant growth differences between in vivo bacterized plants (26.8% for shoot height) and nonbacterized controls were only seen at the 3rd week after inoculation. Compared with the in vitro inoculation, there was no delay in the verticillium wilt symptom expression, even at the lowest concentration of V. dahliae, by in vivo PsJN inoculation. These results suggest that endophytic colonization of tomato tissues is required for the Verticillium-resistance responses. Plant growth promotion preceeds the disease-resistance responses and may depend on the colonization thresholds and subsequent sensitization of hosts.Key words: Pseudomonas sp., plant growth promoting rhizobacterium, Verticillium dahliae, tomato, colonization, plant growth promotion, disease suppression.

scholarly journals In Vitro Evaluation and Genome Mining of Bacillus subtilis Strain RS10 Reveals Its Biocontrol and Plant Growth-Promoting Potential

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1273
Author(s):  
Sajid Iqbal ◽  
Nimat Ullah ◽  
Hussnain Ahmed Janjua
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Genome Analysis ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Subtilis Strain ◽  
Antifungal Metabolites ◽  
Plant Growth Promoting ◽  
Growth Promoting

Recently, crop management has involved excessive use of chemical fertilizers and pesticides, compromising public health and environmental integrity. Rhizobacteria, which can enhance plant growth and protect plants from phytopathogen, are eco-friendly and have been attracting increasing attention. In the current study, Bacillus subtilis RS10 isolated from the rhizosphere region of Cynodon dactylon, inhibited the growth of indicator strains and exhibited in vitro plant growth-promoting traits. A whole-genome analysis identified numerous biosynthetic gene clusters encoding antibacterial and antifungal metabolites including bacillibactin, bogorol A, fengycin, bacteriocin, type III polyketides (PKs), and bacilysin. The plant growth-promoting conferring genes involved in nitrogen metabolism, phosphate solubilization, hydrogen sulfide, phytohormones, siderophore biosynthesis, chemotaxis and motility, plant root colonization, lytic enzymes, and biofilm formation were determined. Furthermore, genes associated with abiotic stresses such as high salinity and osmotic stress were identified. A comparative genome analysis indicated open pan-genome and the strain was identified as a novel sequence type (ST-176). In addition, several horizontal gene transfer events were found which putatively play a vital role in the evolution and new functionalities of a strain. In conclusion, the current study demonstrates the potential of RS10 antagonism against important pathogens and plant growth promotion, highlighting its application in sustainable agriculture.

scholarly journals Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.

2016 ◽  
Vol 68 (2) ◽  
pp. 363-374 ◽  
Author(s):  
Pankaj Kumar ◽  
Ramesh Dubey ◽  
Dinesh Maheshwari ◽  
Yong-Ha Park ◽  
Vivek Bajpai
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Growth Promotion ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Pseudomonas Sp ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

In vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase and urease activities, and utilized lactose and some amino acids. All the isolates were indole acetic acid (IAA) positive, however PPR8 solubilized potassium and zinc along with various other types of inorganic (tricalcium, dicalcium and zinc phosphate) and organic (calcium phytate) phosphates, as well as producing siderophore and ACC deaminase. PPR8 also produced cyanogens, extracellular chitinase, ?-1,3-glucanase, ?-1,4-glucanase and oxalate oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent biocontrol agent, inhibiting the growth of various plant pathogenic fungi. This study reveals the potential of Pseudomonas sp. PPR8 to be used as a good bioinoculant for growth promotion of common bean and for the protection of important legume crops from various deleterious phytopathogens.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Deletion of Rap‐Phr systems in Bacillus subtilis influences in vitro biofilm formation and plant root colonization

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Mathilde Nordgaard ◽  
Rasmus Møller Rosenbek Mortensen ◽  
Nikolaj Kaae Kirk ◽  
Ramses Gallegos‐Monterrosa ◽  
Ákos T. Kovács
Keyword(s):  
Bacillus Subtilis ◽  
Biofilm Formation ◽  
Root Colonization ◽  
Plant Root

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

Evaluation of the phytotoxicity of 2,4-diacetylphloroglucinol and Pseudomonas brassicacearum Q8r1-96 on different wheat cultivars

2021 ◽  
Author(s):  
Mingming Yang ◽  
Linda S Thomashow ◽  
David M Weller
Keyword(s):  
Seed Germination ◽  
Pacific Northwest ◽  
Root Colonization ◽  
The United States ◽  
Wheat Cultivars ◽  
The Pacific ◽  
Root Necrosis ◽  
Growth Promoting ◽  
Pseudomonas Brassicacearum

Pseudomonas brassicacearum Q8r1-96 and other 2,4-diacetylphloroglucinol (DAPG)-producing pseudomonads of the Pseudomonas fluorescens complex possess both biocontrol and growth-promoting properties and play an important role in suppression of take-all of wheat in the Pacific Northwest (PNW) of the United States. However, P. brassicacearum can also reduce seed germination and cause root necrosis on some wheat cultivars. We evaluated the effect of Q8r1-96 and DAPG on the germination of 69 wheat cultivars that have been or currently are grown in the PNW. Cultivars varied widely in their ability to tolerate P. brassicacearum or DAPG. The frequency of germination of the cultivars ranged from 0 to 0.87 and from 0.47 to 0.90 when treated with Q8r1-96 and DAPG, respectively. There was a significant positive correlation between the frequency of germination of cultivars treated with Q8r1-96 in assays conducted in vitro and in the greenhouse. The correlation was greater for spring than for winter cultivars. In contrast, the effect of Q8r1-96 on seed germination was not correlated with that of DAPG alone, suggesting that DAPG is not the only factor responsible for the phytotoxicity of Q8r1-96. Three wheat cultivars with the greatest tolerance and three cultivars with the least tolerance to Q8r1-96 were tested for their ability to support root colonization by strain Q8r1-96. Cultivars with the greatest tolerance supported significantly greater populations of strain Q8r1-96 than those with the least tolerance to the bacteria. Our results show that wheat cultivars differ widely in their interaction with P. brassicacearum and the biocontrol antibiotic DAPG.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals Variovorax sp. Has an Optimum Cell Density to Fully Function as a Plant Growth Promoter

2019 ◽  
Vol 7 (3) ◽  
pp. 82 ◽  
Author(s):  
Oyungerel Natsagdorj ◽  
Hisayo Sakamoto ◽  
Dennis Santiago ◽  
Christine Santiago ◽  
Yoshitake Orikasa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cell Density ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Plant Growth Promoting Bacteria ◽  
Biochemical Tests ◽  
Green Pepper ◽  
Plant Growth Promoter ◽  
Plant Growth Promoting ◽  
Growth Promoting

Utilization of plant growth-promoting bacteria colonizing roots is environmentally friendly technology instead of using chemicals in agriculture, and understanding of the effects of their colonization modes in promoting plant growth is important for sustainable agriculture. We herein screened the six potential plant growth-promoting bacteria isolated from Beta vulgaris L. (Rhizobium sp. HRRK 005, Polaromonas sp. HRRK 103, Variovorax sp. HRRK 170, Mesorhizobium sp. HRRK 190, Streptomyces sp. HRTK 192, and Novosphingobium sp. HRRK 193) using a series of biochemical tests. Among all strains screened, HRRK 170 had the highest potential for plant growth promotion, given its ability to produce plant growth substances and enzymes such as siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively, concomitantly with active growth in a wider range of temperatures (10–30 °C) and pH (5.0–10.0). HRRK 170 colonized either as spots or widely on the root surface of all vegetable seedlings tested, but significant growth promotion occurred only in two vegetables (Chinese cabbage and green pepper) within a certain cell density range localized in the plant roots. The results indicate that HRRK 170 could function as a plant growth promoter, but has an optimum cell density for efficient use.

Earthworm Extracts Facilitate PC12 Cell Differentiation and Promote Axonal Sprouting in Peripheral Nerve Injury

2010 ◽  
Vol 38 (03) ◽  
pp. 547-560 ◽  
Author(s):  
Chao-Tsung Chen ◽  
Jaung-Geng Lin ◽  
Tung-Wu Lu ◽  
Fuu-Jen Tsai ◽  
Chih-Yang Huang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Peripheral Nerves ◽  
Peripheral Nerve Regeneration ◽  
In Vitro Study ◽  
Potential Growth ◽  
Marked Enhancement ◽  
Growth Promoting ◽  
Success Percentage

The present study provides in vitro and in vivo evaluations of earthworm (Pheretima aspergilum) on peripheral nerve regeneration. In the in vitro study, we found the earthworm (EW) water extracts caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as the expressions of growth associated protein 43 and synapsin I. In the in vivo study, silicone rubber chambers filled with EW extracts were used to bridge a 10 mm sciatic nerve defect in rats. Eight weeks after implantation, the group receiving EW extracts had a much higher success percentage of regeneration (90%) compared to the control (60%) receiving the saline. In addition, quantitative histology of the successfully regenerated nerves revealed that myelinated axons in EW group at 31.25 μg/ml was significantly more than those in the controls (p < 0.05). These results showed that EW extracts can be a potential growth-promoting factor on regenerating peripheral nerves.

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