Substrate with Organosuper® for cucumber seedlings formation in protected environments and polystyrene trays

Culture environments, trays and doses of organic compost were evaluated in the formation of cucumber seedlings (Cucumis sativus L.). Five environmental conditions were tested: (A1) a greenhouse with height of 2.5 m, covered with polyethylene film, (A2) nursery with height of 2.5 m, monofilament fabric, 50% shading, (A3) nursery with height of 2.5 m, heat-reflective screen, 50% shading, (A4) nursery with a height of 1.8 m, covered with coconut tree straw and (A5) greenhouse with height of 4.0 m, covered with polyethylene film, with zenith opening and thermo-reflective cloth under the plastic. Trays of 72 (R1) and 128 (R2) cells were filled with 93% soil and 7% organic compound (S1), 86% soil and 14% organic compound (S2) and 79% soil and 21% organic compound (S3). It was used a randomized design in split-split-plot scheme, with five replicates (environments x trays x substrates). The greenhouses provide the best environments for the formation of cucumber seedlings. A tray of 72 cells is the best container, promoting more vigorous seedlings in substrate with soil and 7 or 14% organic compound.

Download Full-text

Assessment of Salicylic Acid Impacts on Seedling Characteristic of Cucumber (Cucumis sativus L.) under Water Stress

Notulae Scientia Biologicae ◽

10.15835/nsb417258 ◽

2012 ◽

Vol 4 (1) ◽

pp. 112-115 ◽

Cited By ~ 3

Author(s):

Hossein MARDANI ◽

Hassan BAYAT ◽

Amir Hossein SAEIDNEJAD ◽

Ehsan Eyshi REZAIE

Keyword(s):

Salicylic Acid ◽

Water Stress ◽

Water Deficit ◽

Cucumis Sativus ◽

Foliar Application ◽

Dry Weight ◽

Water Deficit Stress ◽

Cucumis Sativus L ◽

Water Stress Tolerance ◽

Randomized Design

Impacts of various concentrations of salicylic acid (SA) on cucumber (Cucumis sativus L.) seedling characteristic were evaluated under different water stress levels by using a factorial arrangement based on completely randomized design with three replications at experimental greenhouse of Ferdowsi University of Mashhad, Iran. The studied factors included three water deficit levels (100% FC, 80% FC, and 60% FC) considered as first factor and five levels of SA concentrations (0, 0.25, 0.5, 0.75, and 1 mM) as second factor. Results showed that foliar application of SA at the highest concentration enhanced leaf area, leaf and dry weight while decreased stomatal conductance under high level of water deficit stress. Though, severe water deficit stress sharply raised the SPAD reading values. In general, exogenous SA application could develop cucumber seedling characteristic and improve water stress tolerance.

Download Full-text

A Vacuolar Invertase CsVI2 Regulates Sucrose Metabolism and Increases Drought Tolerance in Cucumis sativus L.

International Journal of Molecular Sciences ◽

10.3390/ijms23010176 ◽

2021 ◽

Vol 23 (1) ◽

pp. 176

Author(s):

Lin Chen ◽

Fenghua Zheng ◽

Zili Feng ◽

Yue Li ◽

Muxuan Ma ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Cucumis Sativus ◽

Sucrose Synthase ◽

Sucrose Phosphate Synthase ◽

Invertase Activity ◽

Cucumis Sativus L ◽

Vacuolar Invertase ◽

Cucumber Seedlings ◽

Sucrose Phosphate

Vacuolar invertase (VI) can irreversibly degrade sucrose into glucose and fructose and involve in plants abiotic-stress-tolerance. Cucumber (Cucumis sativus L.) is susceptible to drought stress, especially during the seedling stage. To date, the involvement of VI in drought tolerance in cucumber seedlings is in urgent need of exploration. In the present study, a cucumber vacuolar invertase gene, CsVI2, was isolated and functionally characterized. The results showed that (1) CsVI2 showed vacuolar invertase activity both in vivo and in vitro; (2) the transcript level of CsVI2, along with VI activity, was significantly induced by drought stress. Moreover, the expression of sucrose synthase 3 (CsSUS3) was increased and that of sucrose phosphate synthase 1 (CsSPS1) was decreased after exposure to drought stress, which was followed by an increase in sucrose synthase activity and a decrease in sucrose phosphate synthase activity; (3) CsVI2-overexpressing transformed cucumber seedlings showed enhanced vacuolar invertase activity and drought tolerance and 4) protein–protein interaction modelling indicated that a cucumber invertase inhibitor, CsINVINH3, can interact with CsVI2. In summary, the results indicate that CsVI2 as an invertase can regulate sucrose metabolism and enhance drought stress in cucumber seedlings.

Download Full-text

Oligotrophic pseudomonads in the rhizosphere: Suppressiveness to Pythium damping-off of cucumber seedlings (Cucumis sativus L.)

Biology and Fertility of Soils ◽

10.1007/bf00336231 ◽

1990 ◽

Vol 9 (3) ◽

pp. 231-234 ◽

Cited By ~ 10

Author(s):

E. E. Sugimoto ◽

H. A. J. Hoitink ◽

O. H. Tuovinen

Keyword(s):

Cucumis Sativus ◽

Damping Off ◽

Cucumis Sativus L ◽

Cucumber Seedlings

Download Full-text

Bioassay for Determining Persistence of the Herbicide Saflufenacil in an Oxisol

Planta Daninha ◽

10.1590/s0100-83582019370100052 ◽

2019 ◽

Vol 37 ◽

Author(s):

F. DIESEL ◽

M.M. TREZZI ◽

R.A. VIDAL ◽

M.V.J. BARANCELLI ◽

P.H.F. MIZERSKI

Keyword(s):

Cucumis Sativus ◽

Beta Vulgaris ◽

Dry Matter ◽

Soil Samples ◽

Cucumis Sativus L ◽

Randomized Design ◽

Completely Randomized Design ◽

Stand Height ◽

Herbicide Effects ◽

Applied Knowledge

ABSTRACT: Persistence of an herbicide is defined as the ability to maintain the integrity of its molecule and chemical, physical, and phytotoxic properties in the environment where it was applied. Knowledge of persistence has implications both for the toxicity generated on species grown in succession and for the period in which the herbicide controls weeds. This study aimed to assess the persistence of the herbicide saflufenacil in an Oxisol by means of bioassays. Two experiments with the application of 29.4 g ha-1 of saflufenacil were carried out under field conditions: the first in the 2011/2012 season and the second in the 2012/2013 season. Soil samples were collected at depths of up to 10 cm at different times after application. Subsequently, two bioassays were carried out in a greenhouse by using beet (Beta vulgaris L.) and cucumber (Cucumis sativus L.) as bioindicators. The studies were carried out in a completely randomized design with four replications. Treatments consisted of periods after saflufenacil application (0, 5, 10, 15, 25, 35, 50, and 100 days). The crop was also considered as a factor. Stand, height, and phytotoxicity of plants were assessed in both agricultural seasons at 14, 21, and 28 days after sowing (DAS), as well as shoot fresh (SFM) and dry matter (SDM) at 28 DAS. In general, saflufenacil persistence was between 25 and 35 days. Beet was more sensitive to the presence of saflufenacil in the soil when compared to cucumber. Herbicide effects were higher in the 2012/2013 season when compared to the 2011/2012 season.

Download Full-text

Reduction of nitrates in Cucumis sativus L. seedlings I. Influence of tungsten and vanadium on absorption and reduction of nitrates

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1980.023 ◽

2014 ◽

Vol 49 (3) ◽

pp. 259-267 ◽

Cited By ~ 1

Author(s):

Józef Buczek ◽

Ewa Kowalińska ◽

Krystyna Kuczera

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Plant Tissues ◽

Inhibiting Activity ◽

Cucumis Sativus L ◽

Cucumber Seedlings

Investigations were conducted on the influence of tungsten (Na2WO4) and meta-vanadate (NaVO3) on uptake and reduction of nitrates by cucumber seedlings. Tungsten and vanadium almost completely inhibited nitrate reductase activity (NR) after treating the plants for 24 hours with nitrates in the presence of 10-4 M WO4-2 and 10-3 M VO3-. Uptake of NO3- in the presence of WO4-2 in this same period of time was lowered by about 50 %, while in the presence of VO3- by seventy percent. The ratio of NO3- absorbed to that reducted in control plants was around 3, whereas in the presence of WO4-2 and VO3- 9 and 8 respectively. The effect of NR inhibiting activity by WO4-2 and VO3- was significant but somewhat weaker if both inhibitors were applied to the plants 24 hours after formation of the NR system, whereas NO3- uptake was subject to a slight lowering. The above data suggest that WO4-2 and VO3- after a 24 hour application of these inhibitors to plants, primary inhibit biosynthesis and activity of NR and to a lesser extent effect NO3- absorption. On the basis of results reached the Butz and Jackson (1977) hypothesis concerning the model of NO3- uptake and reduction by plant tissues is discussed.

Download Full-text

Evaluation of Quitomax® in the production of Cucumis sativus L. in protected culture

Revista de la Facultad de Agronomía, Universidad del Zulia ◽

10.47280/revfacagron(luz).v37.n4.06 ◽

2020 ◽

Vol 37 (4) ◽

pp. 430-441

Author(s):

Yoan Salgado-Valle ◽

Félix Michel Henríquez-Díaz ◽

Miguel Ángel Ramírez-Arrebato ◽

Aida Tania Rodríguez-Pedroso ◽

Michel Ruiz-Sánchez ◽

...

Keyword(s):

Cucumis Sativus ◽

Foliar Application ◽

Cucumis Sativus L ◽

Randomized Design ◽

Plant Stem ◽

Del Rio ◽

Fruit Mass

To evaluate the foliar application of the Quitomax® biostimulant in Cucumis sativus crop, an experiment was developed at the municipality of Pinar del Río province, Cuba, under protected crop conditions. The treatments consisted in applying 300 and 500 mg.ha-1 10 and 25 days after transplantation (ddt) and a commercial Bayfolan Forte® control. A randomized design with four long replicates was used and variables such as height and thickness of the plant stem of Cucumis sativus, number of total fruits, fruit mass and number of exportable quality fruits were measured during five harvest times (32, 39, 46, 60 and 67 ddt). The results showed that the higher concentration of Quitomax® applied (500 mg.ha-1) in the two growth moments produces the highest values for the growth variables and the highest exportable quality fruits.

Download Full-text

The Influence of Colour and Thickness of Nylon Mulch on Soil Temperature, Moisture, Percent Germination and Some Growth Parameters of Cucumber (cucumis sativus L.) Seedlings

Land Science ◽

10.30560/ls.v2n1p13 ◽

2020 ◽

Vol 2 (1) ◽

pp. p13

Author(s):

Kayode Moses Babatunde ◽

Kabiru Alani Shittu ◽

Olusogo Adeyemi Adekanmbi ◽

Mukaila Adetunji Asimi

Keyword(s):

Soil Moisture ◽

Soil Temperature ◽

Plant Height ◽

Cucumis Sativus ◽

Growth Parameters ◽

Cucumis Sativus L ◽

Randomized Design ◽

Percent Germination ◽

Number Of Leaves ◽

Completely Randomized Design

Heat, moisture and oxygen are important factors that has controlled seed germination, thus A 2 ×4 factorial greenhouse experiment was conducted to determine the influence of colour and thickness of nylon mulch on soil temperature, moisture, percent germination and selected growth indicators of cucumber (Cucumis sativus L.) seedlings in a completely randomized design (CRD), where the treatments; nylon colour (white and black) and thicknesses (0, 1.5, 3.0, and 4.5 mm) were replicated three times. The analysis of variance showed that the white nylon thickness had positive significant (P ≤ 0.05) influence on soil temperature, soil moisture, plant height and number of leaves, while black nylon thickness had positive significant (P ≤ 0.05) influence on soil moisture and plant height and a negative significant (P ≤ 0.01) influence on percent germination, it was also discovered that 1.5 mm thickness of the white nylon contributed significantly (p ≤ 0.05) to the parameters measured, therefore, white nylon of 1.5 mm thickness could be recommended as mulch material for cucumber production in a sandy clay soil.

Download Full-text

Genetic Analysis and QTL Mapping of Resistance to Gummy Stem Blight in Cucumis sativus Seedling Stage

Plant Disease ◽

10.1094/pdis-08-16-1116-re ◽

2017 ◽

Vol 101 (7) ◽

pp. 1145-1152 ◽

Cited By ~ 5

Author(s):

Shulin Liu ◽

Yanxia Shi ◽

Han Miao ◽

Min Wang ◽

Baoju Li ◽

...

Keyword(s):

Genetic Analysis ◽

Cucumis Sativus ◽

Recombinant Inbred Lines ◽

Didymella Bryoniae ◽

Cucumis Sativus L ◽

Cucumber Seedlings ◽

Stem Blight ◽

Gummy Stem Blight ◽

Ssr Primers ◽

Important Disease

Gummy stem blight (GSB), caused by Didymella bryoniae (syn. Stagonosporopsis cucurbitacearum), is an important disease of cucumber (Cucumis sativus L.) worldwide. To better understand the resistance to GSB in cucumber seedlings, a set of 160 F9 recombinant inbred lines (RILs) and a total of 405 pairs of SSR primers were employed to detect quantitative trait loci (QTLs) conferring the resistance. Genetic analysis indicated that the resistance to GSB in PI 183967 seedlings was quantitative and mainly governed by two pairs of major QTLs and multiple minor QTLs. Six QTLs, gsb3.1, gsb3.2, gsb3.3, gsb4.1, gsb5.1, and gsb6.1, for resistance to GSB in cucumber seedlings were detected. The stable locus gsb5.1 on Chr.5 was repeatedly detected in three seasons. Locus gsb5.1 accounted for the highest phenotypic variation, 17.9%, and was flanked by SSR15321 and SSR07711 within the genetic distance of 0.5 cM. There were 102 candidate genes predicted in the region harboring the stable QTL gsb5.1, of which seven genes were related to disease resistance. These results can provide a good base for further study and molecular markers for fine-mapping the major QTL conferring GSB resistance in cucumber.

Download Full-text

Response of Cucumis sativus L. seedlings to Pb exposure

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202009000300002 ◽

2009 ◽

Vol 21 (3) ◽

pp. 175-186 ◽

Cited By ~ 6

Author(s):

Jamile F. Gonçalves ◽

Alexssandro G. Becker ◽

Luciane B. Pereira ◽

João B. T. da Rocha ◽

Denise Cargnelutti ◽

...

Keyword(s):

Cucumis Sativus ◽

Soluble Protein ◽

Chlorophyll Concentration ◽

Dry Weight ◽

Total Soluble Protein ◽

Cucumis Sativus L ◽

Cucumber Seedlings ◽

Pb Exposure ◽

Pb Concentration

In this study, the effects of lead (Pb) on growth, photosynthetic pigments concentration, lipid peroxidation, electrolyte leakage percentage (ELP), protein oxidation, aminolevulinate dehydratase (ALA-D; E.C. 4.2.1.24), ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid (AsA), non-protein thiol groups (NPSH) and total soluble protein concentrations in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing three Pb levels as (C2H3O2)Pb.3H2O (0, 100, 400, and 1000 µmol L-1) for 10 d. Increasing Pb concentrations in substrate enhanced Pb concentration in both roots and shoot. Pb accumulated at a higher amount in roots. Root length and total fresh weight were decreased at the two highest Pb concentrations. Cucumber showed no reduction in shoot length and total dry weight at any Pb level. The highest Pb concentration decreased water content and ALA-D activity as well as increased malondialdehyde, carbonyls and total soluble protein concentrations. Carotenoids concentration enhanced at 100 and 400 µmol Pb L-1, while chlorophyll concentration and ELP were not affected by Pb stress. Activity of APX was inhibited while the activities of CAT and SOD were increased at all Pb concentrations. AsA was enhanced at 400 and 1000 µmol Pb L-1 whereas NPSH were increased only at the highest Pb concentration. Therefore, high Pb-exposure caused oxidative stress, and the antioxidant system of the cucumber seedlings was not sufficient to revert it, contributing for growth reduction.

Download Full-text

The effect of ammonium ions on the activity of glutamate dehydrogenase, alanine aminotransferase and aspartate aminotransferase in Cucumis sativus L. seedlings

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1981.066 ◽

2014 ◽

Vol 50 (3) ◽

pp. 429-436

Author(s):

Genowefa Kubiak-Dobosz

Keyword(s):

Glutamate Dehydrogenase ◽

Cucumis Sativus ◽

Aspartate Aminotransferase ◽

Alanine Aminotransferase ◽

Ammonium Ions ◽

Cucumis Sativus L ◽

Factors Affecting ◽

Cucumber Seedlings ◽

Young Leaves ◽

Medium Activity

Changes in the activity of glutamate dehydrogenase (GDH), alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) were studied in various organs of Cucumis sativus L. seedlings in relation to the uptake of mineral nitrogen (in form of N03- or NH4+ ) from the medium. Activity of GDH, GPT, and GOT was higher in young leaves and roots of cucumber seedlings if the plants developed- in an ammonium medium. No similar changes of aminotransferases activity were noted in the cotyledons. Factors affecting varying effect of ammonium ions upon GPT and GOT activity are discussed for particular organs of cucumber seedlings.

Download Full-text