Test of exudates color hues for evaluating the physiological potential of coffee (Coffea arabica L.) seeds

Coffee seeds have slow and irregular germination, losing fast their viability during storage, and the standard germination test of these seeds requires at least 30 days. Besides, the results may not reflect the actual physiological quality of these seeds. The objective of this work was to develop a fast and practical test for evaluating the viability of coffee seeds, which is based on the interpretation of different color hues of exudates from seeds. Coffee seeds of the cultivar Catuai 44 from six lots were submitted to germination, accelerated aging, and electrical conductivity tests. In the exudates color hue test, coffee seeds without the parchment and the silvery pellicle (four replications of 10 seeds each) were distributed on top of paper towels moistened and then maintained into a germinator, at 25 ºC for 24, 48, 72, 96, and 120 h. Three classes of color hues were established: colorless, light color hue, and dark color hue, assigning the values of 0, 1, and 3, for each class, respectively. The proposed exudates color hue test can be recommended for the fast assessment of viability for coffee seeds. The most promising results were obtained for seeds with 12% moisture content, after imbibition periods of 72, 96, and 120 h; and with 30% moisture content, after imbibition periods of 72 and 120 h.

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Foliar application of silicon and the effect on wheat seed yield and quality

Journal of Seed Science ◽

10.1590/s2317-15372013000100012 ◽

2013 ◽

Vol 35 (1) ◽

pp. 86-91 ◽

Cited By ~ 7

Author(s):

Samantha Rigo Segalin ◽

Caroline Huth ◽

Thais D'Avila Rosa ◽

Diógenes Barella Pahins ◽

Liliane Marcia Mertz ◽

...

Keyword(s):

Seed Yield ◽

Foliar Application ◽

Accelerated Aging ◽

Dry Weight ◽

Wheat Cultivars ◽

Wheat Seed ◽

Germination Test ◽

Yield And Quality ◽

Physiological Quality

Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Physiological performance of wheat seeds treated with micronutrients and protection products during storage

Journal of Seed Science ◽

10.1590/2317-1545v39n2176874 ◽

2017 ◽

Vol 39 (2) ◽

pp. 182-188 ◽

Cited By ~ 2

Author(s):

Joice Aline Freiberg ◽

Marcos Paulo Ludwig ◽

Suemar Alexandre Gonçalves Avelar ◽

Eduardo Girotto ◽

Darlan de Maria Eickstedt ◽

...

Keyword(s):

Moisture Content ◽

Seed Treatment ◽

Storage Time ◽

Accelerated Aging ◽

High Quality ◽

Physiological Performance ◽

Physiological Quality ◽

Wheat Seeds

Abstract: Seeds with high quality are one of the important factors to consider in the crops establishment on field. Therefore, the physical and physiological quality of wheat seeds treated with mixes of micronutrients including zinc and protective products were aimed. The wheat seeds were analyzed in a factorial scheme 5x3x4, with five storage time: 0, 60, 120, 180 and 240 days; three micronutrients: micronutrients 1 (1% Mn; 0.1% Mo; 10% Zn); micronutrients 2 (0.3% B; 0.3% Co; 3% Zn) and no micronutrients, and four protective products: untreated seeds, polymer (Colorseed He), fungicide carboxin + thiram (Vitavax® Thiram 200 SC) + insecticide thiamethoxam (Cruiser® 350 FS) and polymer + fungicide + insecticide. After treatment, the seeds were stored under uncontrolled conditions for 240 days and at intervals of 60 days, the germination, accelerated aging and moisture content were determined. Wheat seeds untreated and treated with polymer have the best physiological performance during storage, regardless micronutrients treatments. The wheat seeds treated with carboxin + thiram + thiamethoxam and polymer + carboxin + thiram + thiamethoxam can be storage up to 120 days, under uncontrolled conditions, regardless micronutrients treatments. The seed treatment does not alter the moisture content of wheat seeds.

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Seed quality of rice cultivars stored in different environments

Journal of Seed Science ◽

10.1590/s2317-15372014000100004 ◽

2014 ◽

Vol 36 (1) ◽

pp. 32-39 ◽

Cited By ~ 3

Author(s):

Elizabeth Rosemeire Marques ◽

Eduardo Fontes Araújo ◽

Roberto Fontes Araújo ◽

Sebastião Martins Filho ◽

Plínio César Soares

Keyword(s):

Seed Quality ◽

Accelerated Aging ◽

Storage Period ◽

Rice Cultivars ◽

Germination Test ◽

Randomized Design ◽

Physiological Quality ◽

Completely Randomized Design ◽

And Storage

The objective of this study was to evaluate dormancy and physiological quality of seeds of rice cultivars during storage in different environments. After harvesting, the seeds of three rice cultivars (Seleta, Curinga and Relâmpago) were dried in the sun, to reach moisture content at around 13%. Then, they were packed in paper and stored in four environments: 5 ± 2 ºC / 70 ± 5% RH, 12 ± 2 ºC / 70 ± 5% RH, 18 ± 2 ºC / 65 ± 5% RH and in uncontrolled condition of temperature and relative humidity (natural). Physiological quality was evaluated at the beginning and at 3, 6, 9 and 12 months of storage by germination test, electrical conductivity, accelerated aging and sand emergence. The experiment was conducted in split plots in a completely randomized design with three replications. Environmental factor was applied in the plots, cultivars in the subplots and storage period in subplots. Cultivar Seleta showed higher dormancy, which was surpassed during storage regardless of environment conservation. In general, seeds stored in natural environment showed lower physiological quality. Only the seeds of cultivar Seleta, regardless of the environment, maintained germination above the minimum required for commercialization until six months of storage.

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O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ

ENERGIA NA AGRICULTURA ◽

10.17224/energagric.2021v36n1p28-40 ◽

2021 ◽

Vol 36 (1) ◽

pp. 28-40

Author(s):

Jaquelini Garcia ◽

Cileide Maria Medeiros Coelho

Keyword(s):

Moisture Content ◽

Seed Viability ◽

Mechanical Damage ◽

Accelerated Aging ◽

Seed Vigor ◽

Rice Seed ◽

Randomized Design ◽

Physiological Quality ◽

Drying System

O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ JAQUELINI GARCIA1, CILEIDE MARIA MEDEIROS COELHO1 1Programa de Pós-graduação em Produção vegetal, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brasil, [email protected], [email protected] RESUMO: O grau de umidade na colheita e o sistema de secagem podem ser determinantes na qualidade das sementes produzidas. Neste trabalho foi avaliado se o sistema de secagem e o grau de umidade na colheita interferem na qualidade fisiológica das sementes de arroz. Foi realizado em delineamento inteiramente casualizado em esquema fatorial triplo. Foi utilizada seis cultivares produzidas no Alto Vale do Itajaí/SC na safra 2016/17 com grau de umidade na colheita igual ou superior a 17% (19,30 - 17,30%) e inferior a 17% (16,40 - 15,03%) (base úmida). As sementes foram submetidas aos sistemas de secagem estacionário e intermitente. Realizou-se testes de germinação, viabilidade, vigor pelo envelhecimento acelerado e frio. Sementes colhidas com grau de umidade ≥ 17% apresentaram maior vigor. A cultivar foi um fator de grande relevância na sensibilidade ao dano térmico e mecânico. O sistema de secagem não afetou a viabilidade das sementes, com exceção da cultivar SCS122 Miura que foi sensível a dano térmico e mecânico. A secagem estacionária comprometeu a qualidade fisiológica. Conclui-se que a secagem intermitente e a colheita com grau de umidade igual ou superior a 17%, mantém a qualidade fisiológica das cultivares SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru e SCS121 CL. Palavras-chaves: Secagem estacionária, secagem intermitente, qualidade fisiológica. THE DEGREE OF MOISTURE IN THE HARVEST AND THE DRYING SYSTEM ARE DETERMINANT FOR THE RICE SEED VIGOR ABSTRACT: The degree of moisture at harvest and the drying system can determine the quality of the seeds produced. In this work, it was evaluated whether the drying system and the degree of moisture at harvest affect the physiological quality of rice seeds. It was conducted in a completely randomized design in a triple factorial scheme. Six cultivars produced in Alto Vale do Itajaí/SC in the 2016/17 crop were used, with moisture content at harvest equal or greater than 17% (19.30 – 17.30%) and less than 17% (16,40 - 15,03%) (wet basis). Seeds were submitted to stationary and intermittent drying systems. Tests of germination, viability, vigor by accelerated aging and cold were performed. Seeds harvested with moisture content ≥ 17% showed greater vigor. The cultivar was a factor of great relevance in the sensitivity to thermal and mechanical damage. The drying system did not affect seed viability, except for the cultivar SCS122 Miura, which was sensitive to thermal and mechanical damage. Stationary drying compromised physiological quality. It was concluded that intermittent drying and harvesting with moisture content equal or greater than 17% maintain the physiological quality of the SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru and SCS121 CL cultivars. Keywords: Stationary drying, intermittent drying, physiological quality.

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Exudate coloring test suitability for assessing the viability of coffee seeds ( Coffea arabica L.)

Journal of Seed Science ◽

10.1590/2317-1545v38n3162923 ◽

2016 ◽

Vol 38 (3) ◽

pp. 212-218 ◽

Cited By ~ 1

Author(s):

Paulo César Hilst ◽

Denise Cunha Fernandes dos Santos Dias ◽

Guilherme Fontes Valory Gama ◽

Joyce de Oliveira Araújo

Keyword(s):

Moisture Content ◽

Water Content ◽

Coffea Arabica ◽

Test Results ◽

Germination Test ◽

Paper Towel ◽

Coffea Arabica L ◽

Water Contents

Abstract: The exudate coloring test has been promising in order to quickly evaluate the quality of coffee seeds. The objective of the research was to adjust the coloring exudate test for coffee seeds and to evaluate the influence of the water content of seeds and of the imbibition period on the test results. Seeds from five lots of 'Catuaí 44' were used, with the following water contents: 30%, 20% and 12%. For the exudate coloring test, the parchment and silver skin (spermoderm) from the seeds were removed. Then, the seeds were distributed on a paper towel, moistened with water, and kept in a germinator at 25 °C for 24, 48, 72, 96 and 120 h. Four classes of coloring intensity were established: absence of color (A), light (L), medium (M) and strong (S) intensities, assigning the values 0, 3, 5 and 10 for each class, respectively. The Viability Index (VI) was calculated by the equation VI=100-(0xA)-(3xL)-(5xM)-(10xS). The exudate coloring test may be recommended to estimate the viability of coffee seeds, providing results correlated to the germination test. The best results were obtained for the seeds with 12% moisture content imbibed for 72, 96 and 120 h and seeds with 30% moisture content after 72 and 120 h of imbibition.

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Physiological quality of popcorn seeds assessed by the accelerated aging test

Journal of Seed Science ◽

10.1590/2317-1545v40n4191101 ◽

2018 ◽

Vol 40 (4) ◽

pp. 428-434 ◽

Cited By ~ 2

Author(s):

Cristiane da Silva Rocha ◽

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Heloisa Karoline Kavan ◽

Talis Melo Claudino ◽

...

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Accelerated Aging ◽

Seed Moisture Content ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

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Drying kinetics and physiological quality of ‘Cabacinha’ pepper seeds during storage

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v22n4p292-297 ◽

2018 ◽

Vol 22 (4) ◽

pp. 292-297 ◽

Cited By ~ 1

Author(s):

Hellismar W. da Silva ◽

Luís S. R. Vale Junior ◽

Camila F. Silva ◽

Rannie do C. Souza ◽

Renato S. Soares

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Accelerated Aging ◽

High Moisture ◽

Germination Test ◽

Different Temperatures ◽

Physiological Quality ◽

Drying Curves ◽

And Storage

ABSTRACT Drying air temperature has direct influence on seed quality, especially for vegetable species harvested with high moisture content. This study aimed to determine and model the drying curves of ‘Cabacinha’ pepper seeds (Capsicum chinense L.) at different temperatures, and to evaluate their physiological quality after drying and during storage. The experimental design was completely randomized in a 3 x 3 factorial scheme, with three drying temperatures (35, 38 and 42 °C) and three storage periods (0, 4 and 8 months), with four replicates. Seed quality was evaluated by germination test, first germination count, accelerated aging, electrical conductivity, seedling emergence, and speed of emergence. The Two Terms is the model that best represents the drying curves of ‘Cabacinha’ pepper seeds at temperatures of 35, 38 and 42 °C. Higher germination and vigor values are obtained in seeds dried at 35 and 38 °C. Drying at 42 °C and storage reduce the physiological quality of the seeds.

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Physiological quality of soybean seeds treated with imidacloprid before and after storage

Plant Soil and Environment ◽

10.17221/364/2020-pse ◽

2020 ◽

Vol 66 (No. 10) ◽

pp. 513-518

Author(s):

Renata Cristiane Pereira ◽

Murilo Fuentes Pelloso ◽

Larissa Vinis Correia ◽

Thaisa Cavalieri Matera ◽

Rayssa Fernanda dos Santos ◽

...

Keyword(s):

Electrical Conductivity ◽

Seed Treatment ◽

Seedling Emergence ◽

Accelerated Aging ◽

Soybean Seeds ◽

Germination Test ◽

Soybean Cultivars ◽

Before And After ◽

Physiological Quality

This study aimed to evaluate the effect of different slurry volume of imidacloprid insecticide on the physiological potential during the storage of seeds of three soybean cultivars. A completely randomised experimental design was adopted, in a 6 × 3 × 2 factorial scheme, which treatments were six slurry volumes with imidacloprid insecticide (0, 200, 500, 800, 1 100 and 1 400 mL/100 kg of seeds), 3 soybean cultivars (SYN15630, M5947, and NS5959) and 2 storage periods (0 and 30 days after seed treatment), with four replications. The treated seeds were stored in kraft paper bags with controlled temperature and humidity. The physiological potential of the seeds was evaluated by standard germination test, electrical conductivity, accelerated aging, seedling emergence in the sand seedbed, and the field. The germination and vigor of soybean seeds were reduced during storage, especially with the increase in the volumes of the insecticide solution.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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