scholarly journals Assessment of physiological potential of cucumber seeds using the software Seedling Vigor Imaging System® (SVIS®)

2012 ◽  
Vol 34 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Adrielle Aparecida Chiquito ◽  
Francisco Guilhien Gomes Junior ◽  
Julio Marcos-Filho
Keyword(s):  
Seedling Growth ◽  
Imaging System ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Objective Evaluation ◽  
Seed Vigor ◽  
Cucumis Sativus L ◽  
Seedling Length ◽  
Definition Of

The software Seed Vigor Imaging System (SVIS®), has been successfully used to evaluate seed physiological potential by automated analyses of scanned seedlings. In this research, the efficiency of this system was compared to other tests accepted for assessing cucumber (Cucumis sativus L.) seed vigor of distinct seed lots of Supremo and Safira cultivars. Seeds were subjected to germination, traditional and saturated salt accelerated aging, seedling emergence, seedling length and SVIS analyses (determination of vigor indices and seedling growth uniformity, lengths of primary root, hypocotyl and whole seedlings). It was also determined whether the definition of seedling growth/uniformity ratios affects the sensitivity of the SVIS®. Results showed that analyses SVIS have provided consistent identification of seed lots performance, and have produced information comparable to those from recommended seed vigor tests, thus demonstrating a suitable sensitivity for a rapid and objective evaluation of physiological potential of cucumber seeds. Analyses of four-days-old cucumber seedlings using the SVIS® are more accurate and growth/uniformity does not affect the precision of results.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Seedling image analysis and traditional tests to evaluate onion seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Nayara Roberto Gonçalves ◽  
Silvio Moure Cicero ◽  
Haynna Fernandes Abud
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Onion Seed ◽  
Emergence Percentage

Abstract: The aim of this study was to verify the possibility of using computerized seedling image analysis to detect differences in vigor among onion seed lots and to compare this analysis with traditional vigor tests. We used five seed lots of two onion cultivars, Bella Catarina and Bella Vista. The Seed Vigor Imaging System (SVIS®) software was used to process the seedling images and assess seedling length and vigor through seedling uniformity and growth indexes. These determinations were compared to traditional tests used to evaluate onion seed vigor: germination percentage and first germination count, seedling emergence percentage and emergence speed, accelerated aging, and electrical conductivity. Results showed that vigor differences among onion seed lots can be identified using the SVIS® in a manner similar to traditional vigor tests; assessments should be performed at five days after sowing.

scholarly journals Assessment of the physiological potential of super sweet corn seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho ◽  
Tathiana Silva Timóteo
Keyword(s):  
Seed Quality ◽  
Sweet Corn ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
Corn Seed ◽  
Control Programs ◽  
Seedling Length

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Effect of seed vigor and sowing densities on the yield and physiological potential of wheat seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli
Keyword(s):  
Seed Yield ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Crop ◽  
Seedling Length ◽  
Physiological Quality ◽  
Initial Seed ◽  
Wheat Seeds

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.

scholarly journals Digital images of seedling for evaluating coffee seed vigor

2019 ◽  
Vol 41 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Heiber Andres Trujillo ◽  
Francisco Guilhien Gomes-Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Imaging System ◽  
Primary Root ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Scanned Images ◽  
Coffea Arabica L ◽  
Coffee Seed

Abstract: The digital image analysis of seedlings has become largely employed in seed quality-control programs due to its feasibility, objectivity and fast results. Despite these advantages, no studies have yet demonstrated the efficiency of this technique for analyzing coffee seed vigor. The present study aimed at evaluating the efficiency of the Seed Vigor Imaging System (SVIS®) in determining the vigor of coffee seeds (Coffea arabica L.) from digital images of seedlings. Also, the results of these analyses were contrasted with conventional vigor tests. Six seed lots from each cultivar, Bourbon and Catucaí 20/15, were used. The research was conducted in two experimental times, and the seed vigor was determined by the tests of first germination count, accelerated aging, electrical conductivity, primary root protrusion speed, as well as by the analysis of scanned images of seedlings, made possible by the SVIS® software. For the Bourbon and Catucaí 20/15 cultivars, the SVIS® analysis was able to discriminate lots of both high and low vigor (vigor indexes of 317 to 752, and 181 to 703, respectively). A similar outcome was obtained from the conventional tests appraised in this research. Eventually, it was possible to conclude that the digital analysis of 20-day-old seedlings by SVIS® is an efficient method to evaluate coffee seed vigor.

scholarly journals Assessing the physiological quality of common bean seeds using the Vigor-S® system and its relation to the accelerated aging test

2019 ◽  
Vol 41 (2) ◽  
pp. 187-195 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Laércio Junio da Silva ◽  
Nayara Pereira Capobiango ◽  
Camila Andrade Fialho ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Common Bean ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Growth Index ◽  
Automated Analysis ◽  
Seed Vigor ◽  
Analysis Technique ◽  
Physiological Quality

Abstract: Automated analysis of seed vigor stands out by allowing greater accuracy, standardization, objectivity, and speed in evaluation of the physiological potential of seed lots. The objective of this study was to evaluate the efficiency of the Vigor-S® system in assessing the physiological quality of common bean seeds compared to the information provided by the traditional vigor tests recommended for this species. Four genotypes of common bean were used, each one represented by four seed lots. Characterization of the physiological potential of the lots was carried out by the following tests: germination, first count of germination, seedling emergence, accelerated aging, and electrical conductivity. The results of these tests were compared with the data obtained from the image analysis technique, specifically the Vigor-S® system, which was used to evaluate seedling growth at two, three, and four days after the beginning of the germination test. Shoot length, primary root length, and seedling length were measured, as well as the growth index, uniformity index, and vigor index were calculated. Computerized analysis of seedling images using the Vigor-S® software is a reliable alternative for evaluation the physiological potential of bean seeds, and it produces information similar to evaluations traditionally used for that purpose.

scholarly journals PRODUTIVIDADE E QUALIDADE FISIOLÓGICA DE SEMENTES DE CULTIVARES DE TRIGO EM DIFERENTES DENSIDADES DE SEMEADURA

2018 ◽  
Vol 14 (3) ◽  
pp. 122-131
Author(s):  
Jéssica de Lucena Marinho ◽  
José Henrique Bizzarri Bazzo ◽  
Carolina Pereira Cardoso ◽  
Claudemir Zucareli ◽  
Inês Cristina de Batista Fonseca
Keyword(s):  
Agricultural Research ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Seed Vigor ◽  
Seeding Density ◽  
Seed Productivity ◽  
Seedling Length ◽  
Physiological Quality

Determination of optimum wheat sowing density, adequate to the genotype and the environment, is essential to avoid lodging and competition between plants, which can reduce seed productivity and quality. In this sense, the objective of this study was to evaluate the effect of seeding density (in cultivars BRS Sabiá e BRS Gralha-Azul) on productivity and physiological quality of seeds of two wheat cultivars. The seeds were produced in the experimental field of the Brazilian Agricultural Research Corporation, in Londrina-PR, at the National Center for Soy Research (Embrapa Soja) in experimental design of randomized blocks in a 2x4 factorial scheme, with four replications. The following evaluations were performed: seed productivity, thousand seeds mass, first count of germination, germination, seedling length, seedling dry weight, accelerated aging, seedling emergence in sand and emergency speed index. In general, the increase in seed density increases seed production and does not alter germination and emergence of seedlings of both cultivars. The cultivar BRS Gralha-Azul proved to be more productive than BRS Sabiá. The increase in seeding density increases seed vigor for BRS Sabiá cultivar and reduces to BRS Gralha-Azul cultivar.

scholarly journals Saturated salt accelerated aging and computerized analysis of seedling images to evaluate soybean seed performance

2014 ◽  
Vol 36 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Juliana Tieme Yagushi ◽  
Denis Santiago Costa ◽  
José de Barros França-Neto
Keyword(s):  
Seed Quality ◽  
Imaging System ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Tetrazolium Test

Seed deterioration causes losses to seed companies during the storage. For researches, the challenge has been to develop techniques which improve the seed longevity as well as laboratory analysis methods required for evaluating the evolution of deterioration in storage. This study had the objective of determining the effectiveness of the accelerated aging test with a saturated NaCl solution and Seed Vigor Imaging System (SVIS) analysis in checking soybean seed physiological in comparison with the recommended vigor tests. Soybean seeds from the cultivars BRS 184 and M-Soy 7908 RR were used, each of them were represented by six lots and stored for six months under two environment conditions. Seed physiological quality was checked every two months in storage through germination evaluations, accelerated aging (different methods), tetrazolium test (vigor and viability) seedling emergence, and SVIS analysis. The tetrazolium test is the most advisable method for evaluating soybean seed quality during storage. The results of the saturated salt accelerated aging test with NaCl at 41 ºC for 72 or 96 hours and the SVIS evaluation are also both efficient for estimating the physiological potential of seeds.

scholarly journals Computerized imaging analysis of seedlings for assessment of physiological potential of wheat seeds

2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Relative Humidity ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Cultivars ◽  
Imaging Analysis ◽  
Wheat Seeds

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.

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