scholarly journals Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties

2012 ◽  
Vol 11 (1) ◽  
pp. 102 ◽  
Author(s):  
Zied Zarai ◽  
Ines Chobba ◽  
Riadh Mansour ◽  
Ahmed Békir ◽  
Néji Gharsallah ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ricinus Communis ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Ricinus Communis L

scholarly journals Antitumor Effect of the Essential Oil from the Leaves of Croton matourensis Aubl. (Euphorbiaceae)

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2974 ◽  
Author(s):  
Emilly Lima ◽  
Rafaela Alves ◽  
Gigliola D´Elia ◽  
Talita Anunciação ◽  
Valdenizia Silva ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Cancer Cell ◽  
Hepg2 Cells ◽  
Membrane Integrity ◽  
In Vitro Cytotoxicity ◽  
Cell Cycle Distribution ◽  
Antitumor Effects

Croton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as “orelha de burro”, “maravuvuia”, and/or “sangrad’água”, is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC–MS) and gas chromatography with flame ionization detection (GC–FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included β-caryophyllene, thunbergol, cembrene, p-cymene, and β-elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.

scholarly journals Evaluating the Anti-proliferative Effects of Nanoemulsion Containing Licorice Extract and Lavender Essential Oil on Cancer

2021 ◽  
Vol 24 (1) ◽  
pp. 84-97
Author(s):  
Zohreh Karimi Taheri ◽  
◽  
Mohammad Hosein Aarabi ◽  
Ali Nazari Alam ◽  
Majid Nejati ◽  
...  
Keyword(s):  
Essential Oil ◽  
Cell Lines ◽  
Staphylococcus Epidermidis ◽  
Colorimetric Method ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Drug Formulation ◽  
Study Results ◽  
Licorice Extract

Background and Aim: Despite the anti-cancer and antimicrobial properties of licorice extract and lavender essential oil, some factors, such as low bioavailability and biodegradable, limit their therapeutic use. Using nanoparticles is a method to overcome these restrictions. This study aimed to investigate the anti-proliferative effects of nanoemulsion containing licorice extract and lavender essential oil on cancer cells; we also evaluated its antimicrobial properties in vitro. Methods & Materials: In this experimental study, nanoemulsions, containing licorice extract and lavender essential oil were developed by the spontaneous emulsion method. The anti-proliferative effect of nanoemulsion was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric method on two cell lines HepG2 and SK-MEL-3. To measure the antimicrobial effect of 4 standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Minimum Inhibitory Concentration (MIC) method was used. Ethical Considerations: This study was approved by the Ethics Committee of Kashan University of Medical Sciences (Code: IR.KAUMS.MEDNT.REC.1396.106). Results: The results of MTT test on HepG2 cells indicated that the concentrations of 630, 1250, and 2500 μg/mL nanoemulsions caused toxicity to the cell and led to the death of >50% of the cells (IC50=401μg/mL; P<0.05). Evaluating SK-MEL3 cells revealed that except for 75 μg of nanoemulsion, other concentrations induced death in >50% of the cells (IC50 = 82 μg/mL; P<0.05). In addition, nanoemulsions, with antimicrobial properties, were studied in 4 strains of bacteria; the highest antimicrobial properties were observed in Staphylococcus epidermidis. Conclusion: Nanoemulsion containing licorice extract and lavender essential oil presents antimicrobial and antiproliferative effects on the two cell lines studied. The current study results indicated that the nano emulsification of lavender essential oil and licorice extract can enhance their biological impact; thus, they can be used as a drug formulation.

scholarly journals Chitosan/Essential Oils Formulations for Potential Use as Wound Dressing: Physical and Antimicrobial Properties

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2223 ◽  
Author(s):  
Elaine Pereira dos Santos ◽  
Pedro Henrique Medeiros Nicácio ◽  
Francivandi Coêlho Barbosa ◽  
Henrique Nunes da Silva ◽  
André Luís Simões Andrade ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antimicrobial Properties ◽  
Wound Dressings ◽  
Qualitative Assessment ◽  
Diffusion Method ◽  
Mechanical Resistance ◽  
Film Forming ◽  
Good Transparency ◽  
The Impact

Film-forming emulsions and films, prepared by incorporating different concentrations of clove essential oil (CEO) and melaleuca essential oil (MEO) into chitosan (CS) were obtained and their properties were evaluated. Film-forming emulsions were characterized in terms of qualitative assessment, hydrogen potential and in vitro antibacterial activity, that was carried by the agar diffusion method, and the growth inhibition effects were tested on the Gram-positive microorganism of Staphylococcus aureus, Gram-negative microorganisms of Escherichia coli, and against isolated fungi such as Candida albicans. In order to study the impact of the incorporation of CEO and MEO into the CS matrix, the appearance and thickness of the films were evaluated. Furthermore, Fourier transform infrared spectroscopy (FTIR), contact angle measurements, a swelling test, scanning electron microscopy and a tensile test were carried out. Results showed that the film-forming emulsions had translucent aspect with cloudy milky appearance and showed antimicrobial properties. The CEO had the highest inhibition against the three strains studied. As regards the films’ properties, the coloration of the films was affected by the type and concentration of bioactive used. The chitosan/CEO films showed an intense yellowish coloration while the chitosan/MEO films presented a slightly yellowish coloration, but in general, all chitosan/EOs films presented good transparency in visible light besides flexibility, mechanical resistance when touched, smaller thicknesses than the dermis and higher wettability than chitosan films, in both distilled water and phosphate-buffered saline (PBS). The interactions between the chitosan and EOs were confirmed by. The chitosan/EOs films presented morphologies with rough appearance and with EOs droplets in varying shapes and sizes, well distributed along the surface of the films, and the tensile properties were compatible to be applied as wound dressings. These results revealed that the CEO and MEO have a good potential to be incorporated into chitosan to make films for wound-healing applications.

scholarly journals In vitro establishment of a highly effective method of castor bean (Ricinus communis L.) regeneration using shoot explants

2016 ◽  
Vol 15 (6) ◽  
pp. 1417-1422 ◽  
Author(s):  
Ji-xing ZHANG ◽  
Xiao-yu WANG ◽  
Zi-zhou FENG ◽  
Xue-jun GENG ◽  
Sha-moli MU ◽  
...  
Keyword(s):  
Castor Bean ◽  
Ricinus Communis ◽  
Ricinus Communis L ◽  
Highly Effective ◽  
In Vitro Establishment

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Encapsulation of Babchi Oil in Cyclodextrin-Based Nanosponges: Physicochemical Characterization, Photodegradation, and In Vitro Cytotoxicity Studies

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 169 ◽  
Author(s):  
Sunil Kumar ◽  
Pooja ◽  
Francesco Trotta ◽  
Rekha Rao
Keyword(s):  
Essential Oil ◽  
Physicochemical Characterization ◽  
In Vitro Cytotoxicity ◽  
Traditional Medicines ◽  
Molar Ratios ◽  
Cyclodextrin Nanosponges ◽  
Cytotoxicity Studies ◽  
The Fourier Transform ◽  
Photo Stability

Babchi (Psoralea corylifolia) oil is an important essential oil used in several traditional medicines to cure various disorders. This phytotherapeutic agent possesses a number of pharmacological activities including antibacterial, antifungal, antioxidant, anti-inflammatory, immunomodulatory, and antitumor factors. However, volatile nature, poor stability, and solubility of babchi oil (BO) restrict its pharmaceutical applications. Therefore, the aim of the present work was to encapsulate this oil in β-cyclodextrin nanosponges (NS) in order to overcome the above limitations. To fabricate nanosponges, β-cyclodextrin was cross-linked with diphenyl carbonate in different molar ratios viz. 1:2, 1:4, 1:6, 1:8, and 1:10. The blank nanosponges were loaded with BO using the freeze-drying method. The particle size of the BO loaded nanosponges was found to lie between 200 and 500 nm with low polydispersity index. Furthermore, the zeta potential, the Fourier transform infrared spectroscopy, X-ray diffraction, thermal analysis, and electron microscopy were carried out for characterization of BO nanosponges. Results obtained from spectral analysis ascertained the formation of inclusion complexes. Additionally, solubilisation efficiency of BO was checked in distilled water and found enhanced by 4.95 times with optimized β-cyclodextrin nanosponges. The cytotoxicity study was carried out by the MTT assay using HaCaT cell lines. A significant improvement in photo-stability of essential oil was also observed by inclusion innanosponges. Lastly, the optimized formulation was tested for antibacterial activity using Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Therefore, encapsulation of BO in nanosponges resulted in efficacious carrier system in terms of solubility, photo-stability, and safety of this oil along with handling benefits.

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