Identification of spacewise dependent right-hand side in two dimensional parabolic equation

In this paper numerical solution of the inverse problem of determining a spacewise dependent right-hand side function in two dimensional parabolic equation is considered. Usually, the right-hand side function dependent on spatial variable is obtained from measured data of the solution at the final time point. Many mathematical modeling problems in the field of physics and engineering will encounter the inverse problems to identify the right-hand terms. When studying an inverse problem of identifying the spacewise dependent right-hand function, iterative methods are often used. We propose a new conjugate gradient method based on the constructed self-adjoint operator of the equation for numerical solution of the function and numerical examples illustrate the efficiency and accuracy.

Peri-Implantitis: Application of a Protocol for the Regeneration of Deep Osseous Defects. A Retrospective Case Series

Background and aims: Peri-implantitis is a complex pathology, both in its diagnosis and in the identification of etiological causes. Although we have been studying more and more over the years to try to answer the many questions that remain regarding everything that circulates around this disease which affects implants, nothing has yet been taken as an official consensus regarding its surgical treatment. There are still many proposed protocols, each of which has been shown to have comforting results and promising prospects, but no total predictability. The aim of this case series is to assess the clinical outcomes of a mixed protocol for the regeneration of deep osseous defects. Materials and methods: The data and clinical records of 23 patients, with 29 implants affected by peri-implantitis treated surgically in private practice, were analyzed retrospectively. The method used for the surgical treatment was a mixed protocol of mechanical–chemical decontamination and bone regeneration with bovine xenograft. Results: All patients were followed for at least 2 years, averaging 28.9 months (a range of 24–38 months) with a reduction in the probing depth (PD) at one year from the initial 8.14 ± 1.156 mm to 3.72 ± 0.649 mm, and to 4.14 ± 1.093 mm at the final assessment. The differences between assessment time points were always statistically significant. The data regarding bleeding on probing (BoP) and suppuration also showed a statistically significant reduction at the final time point compared to the baseline. Only one patient, at 24 months, still showed BoP, suppuration, and a PD deeper than 5 mm, indicating a recurrence of the disease compared to the initial improvement of the PD (5 mm) at one year. Conclusions: In conclusion, within the limits of this retrospective analysis, it can be affirmed that this combined mechanical–chemical and regenerative decontamination therapy is effective in the treatment of peri-implantitis.

Now for the Good News: Self-Perceived Positive Effects of the First Pandemic Wave on Romantic Relationships Outweigh the Negative

Media attention has highlighted the COVID-19 pandemic’s negative effects on romantic relationships (e.g., increased partner aggression). The current mixed-method study also explored potential positive effects, and how the relative balance of positive versus negative effects might have changed over time during the first pandemic wave. Individuals ( N = 186) who participated in a pre-COVID study were recruited through MTurk to participate in a four-wave longitudinal follow-up, every 2 weeks from mid-April to late May 2020. Participants completed an 8-item self-report measure assessing perceived negative and positive effects of the pandemic on their romantic relationship. Multi-level models revealed that perceived positive effects were substantially higher than perceived negative effects at each timepoint, even amongst those who reported being more heavily impacted by the pandemic. Both positive and negative effects were stable across time. Open-ended questions at the final time point were coded for common themes. Positive themes were more frequent than negative themes. The most common negative theme centered on increased stress or tension in the relationship, while the most common positive theme discussed the importance of focusing on and appreciating the relationship, including taking advantage of the gift of increased time together the pandemic had brought. Amongst all of the pandemic’s bad news, it is refreshing to consider the possibility of pandemic-related benefits for people’s romantic relationships.

Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract: A Randomized, Crossover, Double-Blind, and Placebo-Controlled Study

Spore-based probiotics offer important advantages over other probiotics as they can survive the harsh gastric conditions of the stomach and bile salts in the small intestine, ultimately germinating in the digestive tract. A novel clinical trial in 11 ileostomy participants was conducted to directly investigate the presence and germination of the probiotic strain Bacillus subtilis DE111® in the small intestine. Three hours following ingestion of DE111®, B. subtilis spores (6.4 × 104 ± 1.3 × 105 CFU/g effluent dry weight) and vegetative cells (4.7 × 104 ± 1.1 × 105 CFU/g effluent dry weight) began to appear in the ileum effluent. Six hours after ingestion, spore concentration increased to 9.7 × 107 ± 8.1 × 107 CFU/g and remained constant to the final time point of 8 h. Vegetative cells reached a concentration of 7.3 × 107 ± 1.4 × 108 CFU/g at 7 h following ingestion. These results reveal orally ingested B. subtilis DE111® spores are able to remain viable during transit through the stomach and germinate in the small intestine of humans within 3 h of ingestion.

A Little Good News - Relationships During Early Stages of COVID-19 Pandemic

Media attention has highlighted the Covid-19 pandemic’s negative effects on romantic relationships (e.g., increased partner aggression). The current mixed-method study also explored potential positive effects, and how the relative balance of positive versus negative effects might have changed over time during the first pandemic wave. Individuals (N = 186) who participated in a pre-COVID study were recruited through MTurk to participate in a four-wave longitudinal follow-up, every two weeks from mid-April to late May 2020. Participants completed an 8-item self-report measure assessing perceived negative and positive effects of the pandemic on their romantic relationship. Multi-level models revealed that perceived positive effects were substantially higher than perceived negative effects at each timepoint, even amongst those who reported being more heavily impacted by the pandemic. Both positive and negative effects were stable across time. Open-ended questions at the final time point were coded for common themes. The most common negative theme centered on increased stress or tension in the relationship, while the most common positive theme discussed the importance of focusing on and appreciating the relationship, including taking advantage of the gift of increased time together the pandemic had brought. Amongst all of the pandemic’s bad news, it is refreshing to consider the possibility of pandemic-related benefits for people’s romantic relationships.

Comparative Pharmacokinetic Study of Forchlorfenuron in Adult and Juvenile Rats

Forchlorfenuron (CPPU) is a plant growth regulator extensively used in agriculture. However, studies on CPPU pharmacokinetics are lacking. We established and validated a rapid, sensitive, and accurate liquid chromatography–mass spectrometry method for CPPU detection in rat plasma. CPPU pharmacokinetics was evaluated in adult and juvenile rats orally treated with 10, 30, and 90 mg/kg of the compound. The area under the plasma drug concentration–time curve from 0 to 24 h (AUC), at the final time point sampled (AUC0–t), and the maximum drug concentration of CPPU increased in a dose-dependent manner. The pharmacokinetic parameters AUC0–t and absolute bioavailability were higher in the juvenile rats than in adult rats. The mean residence time and AUC0–t of juvenile rats in the gavage groups, except for the 10 mg/kg dose, were significantly higher in comparison to those observed for adult rats (p < 0.001). The plasma clearance of CPPU in juvenile rats was slightly lower than that in the adult rats. Taken together, juvenile rats were more sensitive to CPPU than adult rats, which indicates potential safety risks of CPPU in minors.

Exposure duration modulates the response of Caribbean corals to global change stressors

Global change is threatening coral reefs, with rising temperatures leading to repeat bleaching events (dysbiosis of coral hosts and their symbiotic algae) and ocean acidification reducing net coral calcification. Although global-scale mass bleaching events are revealing fine-scale patterns of coral resistance and resilience, traits that lead to persistence under environmental stress remain elusive. Here, we conducted a 95-day controlled-laboratory experiment to investigate how duration of exposure to ocean warming (28, 31°C), acidification (pCO2 = 400–2800 μatm), and their interaction influence the physiological responses of two Caribbean reef-building coral species (Siderastrea siderea, Pseudodiploria strigosa) from two reef zones of the Belize Mesoamerican Barrier Reef System. Every 30 days, calcification rate, total host protein and carbohydrate, chlorophyll a pigment concentration, and symbiont cell density were quantified for the same coral colony to characterize acclimatory responses of each genotype. Physiologies of the two species were differentially affected by these stressors, with exposure duration modulating responses. Siderastrea siderea was most affected by extreme pCO2 (~2800 μatm), which resulted in reduced calcification rate, symbiont density, and chlorophyll a concentration. Siderastrea siderea calcification rate initially declined under extreme pCO2 but recovered by the final time point, and overall demonstrated resistance to next-century pCO2 and temperature stress. In contrast, P. strigosa was more negatively impacted by elevated temperature (31°C). Reductions in P. strigosa calcification rate and total carbohydrates were consistently observed over time regardless of pCO2 treatment, with the greatest reductions observed under elevated temperature. However, nearshore colonies of P. strigosa maintained calcification rates under elevated temperature throughout all exposure durations, suggesting individuals from this environment may be locally adapted to the warmer temperatures characterizing their natal reef zone. This experiment highlights how tracking individual coral colony physiology across broad exposure durations can capture acclimatory responses of corals to global change stressors.

Effect of Kelulut Honey on the Cellular Dynamics of TGFβ-Induced Epithelial to Mesenchymal Transition in Primary Human Keratinocytes

Over-induction of epithelial to mesenchymal transition (EMT) by tumor growth factor beta (TGFβ) in keratinocytes is a key feature in keloid scar. The present work seeks to investigate the effect of Kelulut honey (KH) on TGFβ-induced EMT in human primary keratinocytes. Image analysis of the real time observation of TGFβ-induced keratinocytes revealed a faster wound closure and individual migration velocity compared to the untreated control. TGFβ-induced keratinocytes also have reduced circularity and display a classic EMT protein expression. Treatment of 0.0015% (v/v) KH reverses these effects. In untreated keratinocytes, KH resulted in slower initial wound closure and individual migration velocity, which sped up later on, resulting in greater wound closure at the final time point. KH treatment also led to greater directional migration compared to the control. KH treatment caused reduced circularity in keratinocytes but displayed a partial EMT protein expression. Taken together, the findings suggest the therapeutic potential of KH in preventing keloid scar by attenuating TGFβ-induced EMT.

Comparison of High Intensity Accelerated Corneal Cross Linking Protocols in Treatment of Progressive Keratoconus;

Purpose: To compare the safety and efficacy of Accelerated Corneal Cross Linking (AXL) protocols ,9mW/cm2 for 10 min with 18mW/cm2 for 5 min in terms of refractive and topographic keratometric indices in patients with progressive keratoconus over 3, 6, 12 and 18 months follow-up. Study Design: Quasi experimental study. Place and Duration of Study: Armed Forces Institute of Ophthalmology Rawalpindi Pakistan, from Nov 2016 to Jun 2018. Material and Methods: Sixty eyes, 30 in each group, of 55 consecutive patients diagnosed with progressive keratoconus were enrolled through convenience sampling and were subjected to AXL with irradiance protocols of 18 watt/cm2 for 5 minutes in group 1 and 9mwatt/cm2 for 10 minutes in group 2. All patients underwent a comprehensive ophthalmic examination at baseline and postoperative follow up visits at 3, 6, 12 and 18 months. Primary outcome parameter was disease stability defined as increase in maximum keratometry over baseline K max of no more than 1 diopter at 1 year after AXL. Statistical analysis of data was performed with IBM SPSS software (version 20.0 SPSS). P value of < 0.05 was considered as statistically significant. Results: Disease stability was 96 % in each group. At the final time point of 18 months, group 2 (AXL 9mmwatt/cm2 for 10 min) was superior as compared to group 1 (AXL 18mmwatt/cm2 for 5 min) in terms of flattening of steep and sim K (p = 0.007, 0.023 respectively). Conclusion: The two AXL protocols are safe and appear to show comparable efficacy in disease stability. They can be used alternatively in the treatment of progressive keratoconus.

Attention, Speech-Language Dissociations, and Stuttering Chronicity

Summary The purpose of this study was to investigate the singular and joint contributions of speech-language dissociations and attention (i.e., distractibility and attention span) to stuttering chronicity. Method Participants, aged 3;0–4;11 (years;months) at an initial visit, were classified as persisting ( n = 10; 9 boys), recovered ( n = 32; 23 boys), and nonstuttering ( n = 28; 19 boys) based on multiple speech and language evaluations spread across 2 years. The evaluations included assessments of articulation, receptive and expressive vocabulary, and omnibus receptive and expressive language. These measures were used to identify speech-language dissociations using a correlation-based statistical approach. Attentional characteristics, which included measures of distractibility and attention span, were based on parent report. Analyses investigated between-group differences related to dissociations and attentional characteristics as well as the relation between these indices. Results There were no significant between-group differences for the persisting and recovered groups on measures of speech-language dissociations; however, the recovered group was found to exhibit less optimal attention span than the persisting group. In addition, children with dissociations exhibited less optimal distractibility and attention spans at the final time point than children without dissociations. Conclusions Present results indicate that attention is related to both stuttering chronicity and the presence of speech-language dissociations; however, they do not support the notion that dissociations are associated with stuttering persistence. These results provide novel insights into the complex nature of the association between developmental stuttering, speech-language dissociations, and attention.