scholarly journals Temporal expression pattern of the insulin-like growth factor II and fibroblast growth factor transcripts in avian embryogenesis

2008 ◽  
Vol 51 (5) ◽  
pp. 949-955
Author(s):  
Jane Eyre Gabriel ◽  
Lúcia Elvira Alvares ◽  
Luiz Lehmann Coutinho
Keyword(s):  
Growth Factor ◽  
Expression Pattern ◽  
Pcr Analysis ◽  
Fibroblast Growth ◽  
Temporal Expression ◽  
Rt Pcr ◽  
In Ovo ◽  
Chicken Embryos ◽  
Factor Ii ◽  
Temporal Expression Pattern

In this study, the abundance of IGF-II and bFGF transcripts was estimated in the chicken embryos using the competitive RT-PCR analysis. Significant enhancements in the abundance of IGF-II mRNA were observed at stages HH1 and 5, and a new accumulation in these levels was observed at stage HH18 in comparison to the basal levels. The abundance of bFGF mRNA increased significantly at stages HH18 and 20, followed by an upregulation in the expression of these transcripts at stage HH26. These findings provided important information about the temporal expression pattern of IGF-II and bFGF transcripts in the whole chicken embryos during in ovo development.

scholarly journals The spatial and temporal expression pattern of Nesp and its antisense Nespas, in mid-gestation mouse embryos

2001 ◽  
Vol 100 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Simon T. Ball ◽  
Christine M. Williamson ◽  
Christopher Hayes ◽  
Terry Hacker ◽  
Jo Peters
Keyword(s):  
Expression Pattern ◽  
Mouse Embryos ◽  
Temporal Expression ◽  
Temporal Expression Pattern

Pirfenidone inhibits PDGF isoforms in bleomycin hamster model of lung fibrosis at the translational level

1999 ◽  
Vol 276 (2) ◽  
pp. L311-L318 ◽  
Author(s):  
G. Gurujeyalakshmi ◽  
M. A. Hollinger ◽  
S. N. Giri
Keyword(s):  
Growth Factor ◽  
Lung Fibrosis ◽  
Platelet Derived Growth Factor ◽  
Pcr Analysis ◽  
Saline Control ◽  
Protective Effects ◽  
Rt Pcr ◽  
Hamster Model ◽  
Pdgf Isoforms ◽  
Translational Level

Pirfenidone (PD) is known for its antifibrotic effects in the bleomycin (BL) hamster model of lung fibrosis. We evaluated whether pretreatment of hamsters with PD could influence the effects of BL-induced overexpression of platelet-derived growth factor (PDGF)-A and PDGF-B genes and proteins in the same model of lung fibrosis. We demonstrate elevated levels of PDGF-A and PDGF-B mRNAs in bronchoalveolar lavage (BAL) cells from lungs of BL-treated compared with saline control hamsters by RT-PCR analysis. However, these levels were not altered in BAL cells obtained from BL-treated hamsters on diets containing 0.5% PD. Western blot analysis of BAL fluid for PDGF isoforms demonstrated that PD treatment inhibited the synthesis of both PDGF-A and PDGF-B isoforms. PD treatment also decreased the mitogenic activity in the BAL fluid from BL-treated hamster lungs. Taken together, these data provide evidence that the protective effects of PD against BL-induced lung fibrosis may be mediated by a reduction in PDGF isoforms produced by lung macrophages.

scholarly journals Gene expression of growth factor BMP15, GDF9, FGF2 and their receptors in bovine follicular cells

2018 ◽  
pp. 6778-6787 ◽  
Author(s):  
Pablo S Reineri ◽  
María S. Coria ◽  
María G. Barrionuevo ◽  
Olegario Hernández ◽  
Santiago Callejas ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Growth Factor Receptor ◽  
Follicular Development ◽  
Fibroblast Growth ◽  
Rt Pcr ◽  
Follicular Cells

Introduction. Growth and follicular maturation involve transformations of various components of the follicle, such as the oocyte, granulosa and techa cells. Several growth factors, including differentiation growth factor 9 (GDF9), bone morphogenic protein 15 (BMP15) and basic fibroblast growth factor (FGF2) are important for follicular development and oocyte maturation, by its ability to increase the proliferation of granulosa, techa cells and the ovarian stroma. Objetive. Evaluate mRNA expression of GDF9, BMP15, FGF2 and their main receptors, transforming growth factor beta receptor 1 (TGFβ-R1), bone morphogenetic protein receptor, type IB (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) in bovine follicular cells. Materials and methods. Total RNA was isolated from pooled samples of oocytes (OOs), cumulus cells (CCs) of cumulus oocyte complexes (COCs) and follicular cell pellets (PCs) of 70 ovaries obtained from 96 beef heifers, collected at a local abattoir. The expression pattern of growth factors and their receptors in follicular bovine cells was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Results. The mRNA transcripts encoding GDF9, BMP15, FGF2, TGFβ-R1, BMPR-IB and FGFR2 genes were detected, by RT-PCR, in all studied cells. This is the first time that the expression of TGFβ-R1 and BMPR-IB receptors is reported in bovine oocytes. Conclusions. The presence of growth factors and receptor transcripts in the studied cells indicate that these factors could act as paracrine and autocrine regulators of folliculogenesis.

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