scholarly journals Gills of juvenile fish piaractus mesopotamicus as histological biomarkers for experimental sub-lethal contamination with the Organophosphorus Azodrin®400

2009 ◽  
Vol 52 (6) ◽  
pp. 1431-1441 ◽  
Author(s):  
Cácia Aparecida Mendes Rudnicki ◽  
Gisele Cristiane Melo ◽  
Lucélia Donatti ◽  
Helena Gonçalves Kawall ◽  
Edith Fanta
Keyword(s):  
Growth And Development ◽  
Juvenile Fish ◽  
Lethal Dose ◽  
Water Bodies ◽  
Native Fish ◽  
Active Principle ◽  
Gaseous Exchange ◽  
Piaractus Mesopotamicus ◽  
Pillar Cells ◽  
Important Time

Piaractus mesopotamicus is a freshwater native fish from rivers of the Paraná-Paraguay Basin and of the Pantanal region and has been used for repopulation programs in Brazil. Juvenile fishes were exposed to the sub-lethal dose of 1.08mg/L of the OP Azodrin®400 containing 0.43µL/L of the active principle monocrotophos for 96 h. A frequent pathology in the gills at all times of exposure was epithelial detachment, from minimal until 24 h of exposure, to intense after 48 h of contamination. Deformed pillar cells in the respiratory lamellae leading to irregular blood spaces and blood congestion, as well as hyperplasia and lamellar fusion were observed. These histopathologies suggested that 48 h after T0 was an important time when a reduction in the capability for gaseous exchange with consequent weakening of the fishes' condition could occur. This could impair growth and development of juveniles introduced in water bodies for repopulation programs.

Dependence of efficiency of natural fish reproduction in water bodies of Kazakhstan from water level

2021 ◽  
pp. 32-41
Author(s):  
K.B. Isbekov ◽  
E.V. Kulikov ◽  
S.Zh. Asylbekova
Keyword(s):  
Water Content ◽  
Water Regime ◽  
Juvenile Fish ◽  
Hydrological Regime ◽  
Water Bodies ◽  
Fish Reproduction ◽  
Data Set ◽  
Commercial Fish ◽  
Level Of Significance ◽  
The Impact

The article deals with the relationship between the hydrological regime of water bodies and the efficiency of fish reproduction. The hydrological regime of water bodies in years of different water content influences the reproduction and, accordingly, the quantitative and qualitative composition of ichthyocenoses. The easiest way to assess the efficiency of fish reproduction is by such an indicator as the productivity of juveniles, i.e. the number of juvenile fish per unit area or volume. The material for this work was carried out in large fishing reservoirs of the Republic of Kazakhstan in the process of fishery research (2013–2018). Comparison of hydrological parameters and productivity of juveniles by years shows their relationship. Correlation coefficient between water content (runoff) of the river Zhaiyk and juvenile yield +0.73 (data set for 11 years, reliable for the level of significance p = 0.01). The yield of juvenile fish from Lake Balkhash has a high correlative dependence on the inflow of water into the lake (r = +0.826) (massif 2013–2018, reliable for the level of significance p = 0.05). Correlation analysis showed that the productivity of juvenile fish in the Kapshagai reservoir has an average degree of dependence on water content (average annual level) (r = +0.641, reliable for the level of significance p = 0.05). Regular relationships between the water regime and the efficiency of reproduction (productivity of juveniles) of fish have been established. The efficiency of natural reproduction of commercial fish species in modern conditions of the water regime, water withdrawal for economic needs and the impact of fishing is significantly reduced, but with the implementation of the reclamation measures proposed in the work, its partial restoration is possible.

scholarly journals The off-host survival and viability of a native and non-native fish louse (Argulus, Crustacea: Branchiura)

2011 ◽  
Vol 57 (6) ◽  
pp. 828-835 ◽  
Author(s):  
P.D. Walker ◽  
I.J. Russon ◽  
R. Duijf ◽  
G. Van Der Velde ◽  
S.E. Wendelaar Bonga
Keyword(s):  
Invasive Species ◽  
Survival Time ◽  
Developmental Stages ◽  
Water Bodies ◽  
Effect Of Temperature ◽  
Native Fish ◽  
Abiotic Conditions ◽  
Clear Effect ◽  
Access To Food ◽  
Fish Louse

Abstract Fish ectoparasites are introduced to water bodies or aquaculture systems along with their hosts. Argulid fish lice can survive off-host for a given time, and in spite of quarantine measures might also be introduced via the transfer of water. However, they must cope with varying abiotic conditions without access to food. We investigated the effect of temperature (5 to 28oC) on the off-host survival time of Argulus japonicus, an invasive species which is apparently increasing its distribution, and compared this with the European A. foliaceus. A clear effect of temperature on the off-host survival time of all stages of both species was demonstrated. Larval and juvenile A. japonicus survived up to 9 days at 22oC and in the case of adults up to 13 days at 15oC. By comparison, larval A. foliaceus survived up to 5 days at 15oC, adults up to 14 days at 9oC and juveniles up to 7 days at 9oC and 15oC. Thus, A. japonicus is more resistant to starvation at higher temperatures under controlled off-host conditions and A. foliaceus is more resistant to starvation at lower temperatures. Infectivity of A. japonicus decreased linearly with the time spent off-host after 2 days for larvae and 4 days for adults. Temperature only had a significant effect on the infectivity of both developmental stages after 24 hours off-host between 13–23oC for larvae and 13–18oC for adults. In conclusion both species can potentially survive off-host periods in water transports for up to 13–14 days as adults. Their survival is shorter as larvae. This study demonstrates that transportation of water has the potential to introduce Argulus species.

scholarly journals TOXICIDADE AGUDA DO INSETICIDA PARATION METÍLICO E DO BIOPESTICIDA AZADIRACTINA DE FOLHAS DE NEEM (Azadirachta indica) PARA ALEVINO E JUVENIL DE PACU (Piaractus mesopotamicus)

2004 ◽  
Vol 14 ◽  
Author(s):  
CLAUDINEI CRUZ ◽  
JOAQUIM G. MACHADO-NETO ◽  
MANOEL LIMA DE MENEZES
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Environmental Risk ◽  
Azadirachta Indica ◽  
Methyl Parathion ◽  
Juvenile Fish ◽  
Aquatic Environments ◽  
Piaractus Mesopotamicus ◽  
Neem Leaves

Os objetivos deste trabalho foram calcular a CL (I) 50-96h do inseticida organofosforado paration metílico e do biopesticida azadiractina para alevinos e juvenis de pacu ( P. mesopotamicus). Também pretendeu-se avaliar o efeito do peso corpóreo sobre a toxicidade aguda do paration metílico e do neem para o pacu, bem como classificar o risco ambiental do uso de paration metílico e do neem para o controle de parasitas e patógenos de pacu. Foram realizados dois experimentos, em condições laboratoriais, para a determinação da concentração letal (CL (I) 50-96h). A CL (I) 50-96h calculada do paration metílico foi de 3,97 mg/L para os alevinos e de 9,89 mg/L para os juvenis. Para a azadiractina foi de 1,20 mg/L para os alevinos e de 1,18 mg/L para os juvenis. As concentrações de 1,0 mg/L de paration metílico para os alevinos e de 7,5 mg/L para os juvenis e as de 0,29 e 0,59 mg/L de azadiractina não provocaram mortalidade nos animais expostos e podem ser utilizadas como referência em estudos de controle de parasitas em pacu . O paration metílico foi menos tóxico para os alevinos e para os juvenis de pacu do que a azadiractina, indicando a necessidade de cuidados com a utilização de extratos aquosos de neem no ambiente aquático. ACUTE TOXICITY OF THE INSECTICIDE METHYL PARATHION AND OF THE BIOPESTICIDE AZADIRACHTIN FROM NEEM LEAVES (Azadirachta indica) TO ALEVINE AND JUVENILE PACU (Piaractus mesopotamicus) Abstract The objectives of the present study were: to calculate the lethal concentration LC (I) 50-96 h of methyl parathion and of the biopesticide azadirachtin to alevine and juvenile pacu ( P. mesopotamicus). Also to determine the effect of body weight on acute toxicity of methyl parathion and of neem for pacu, as well as to classify the environmental risk of the methyl parathion and neem uses for the control of pacu parasites and pathogens. Two experiments were performed under laboratory conditions for the determination of the LC (I) 50-96 h. The LC (I) 50-96 h of methyl parathion was 3.97 mg/L for alevine and 9.89 mg/L for juvenile fish. The LC (I) 50-96 h of azadirachtin was 1.20 mg/L for alevine and 1.18 mg/L for juvenile pacu. Concentrations of 1.0 and 7.5 mg/L of methyl parathion and of 0.29 and 0.59 mg/L of azadirachtin for alevine and juvenile pacu, respectively, did not cause mortality in the exposed animals and can be used as references in studies on the control of parasites in pacu. Methyl parathion was less toxic to alevine and juvenile pacu than azadirachtin, indicating the need for careful utilization of aqueous neem extracts in aquatic environments.

scholarly journals Growth and development of the green toad (Bufo viridis) from the water bodies of the oil producing regions of Kazakhstan

2018 ◽  
Vol 2 (55) ◽  
pp. 111-120
Author(s):  
L.R. Sutuyeva ◽  
◽  
T.M. Shalakhmetova ◽  
V.L. Trudeau ◽  
S.Zh. Kolumbayeva ◽  
...  
Keyword(s):  
Growth And Development ◽  
Water Bodies ◽  
Bufo Viridis ◽  
Green Toad ◽  
Toad Bufo

scholarly journals Estimation of natural reproduction efficiency of semi-anadromous and river fish species in inland water bodies of the Republic of Dagestan, Russia

2020 ◽  
Vol 15 (3) ◽  
pp. 31-42
Author(s):  
A. S. Abdusamadov ◽  
E. A. Akhmaev ◽  
A. A. Latunov ◽  
T. A. Abdusamadov ◽  
A. K. Butaeva ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Juvenile Fish ◽  
Relative Proportion ◽  
Water Bodies ◽  
Migration Routes ◽  
Inland Water ◽  
Natural Reproduction ◽  
Fish Juveniles ◽  
Inland Water Bodies ◽  
Reproduction Efficiency

Aim. Estimation of the natural reproduction efficiency and patterns of formation of fish stocks.Material and Methods. Collection of material was carried out in 2010-2019 in inland water bodies in Dagestan and its shallow Caspian Sea coastal zone. Estimation of the number of juveniles in rivers and canals was carried out using ichthyoplankton cone seines. Fry seines were used to count juveniles of fish in water bodies and in the coastal area. 470 samples of juveniles were collected and 5640 juveniles were analysed. The collection and processing of materials were carried out according to generally accepted methods.Results. The main environmental factors determining the productivity of fish juveniles in the study area in the long-term have been identified. During the observation period, juvenile fish of 15 species were recorded in spawning grounds of inland water bodies. Roach, bream, carp, crucian carp, rudd and vimba bream dominate. In terms of numbers, roach juveniles predominate: their relative proportion being 31.02% with an increase from 27.1% in 2010 to 35.4% in 2018.The second place was occupied by bream juveniles, whose average relative proportion was 12.1%. The third place was occupied by carp juveniles with an average proportion of 9%. Juvenile zander, catfish, pike and kutum are relatively low in abundance from 2 to 4%. The yield of juvenile fish in 2010-2019 decreased 1.7 times compared to 1990-1995.Conclusion. It was established that a decrease in the number of juvenile fishes occurs under the influence of unfavorable environmental factors: decrease in sea level; reduction in spawning areas; the shallowing and silting of migration routes due to the lack of a stable water supply. Measures are proposed to improve the living conditions of aquatic biological resources, aimed at increasing the efficiency of their natural reproduction.

Effects of dietary aflatoxin on biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish

2021 ◽  
pp. 1-10
Author(s):  
E. C. Michelin ◽  
C. M. Bedoya-Serna ◽  
L. C. S. Carrion ◽  
N. Levy-Pereira ◽  
F. S. Cury ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Juvenile Fish ◽  
Fatty Degeneration ◽  
Hepatic Tissue ◽  
Blood Collection ◽  
Fish Feed ◽  
Hydropic Degeneration ◽  
Piaractus Mesopotamicus ◽  
Liver Changes ◽  
Aflatoxin B

The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control – feed without toxin; Treatment A – feed + 10 μg aflatoxin B1 (AFB1)/kg; Treatment B – feed + 20 μg AFB1/kg; and Treatment C – feed + 50 μg AFB1/kg. Matrinxã and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxã, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxã it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxã, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB1.

scholarly journals Autochthonous probiotic mixture improves biometrical parameters of larvae of Piaractus mesopotamicus (Caracidae, Characiforme, Teleostei)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Marcos Gabriel Guidoli ◽  
Jorge Arnaldo Mendoza ◽  
Sofia Lizardo Falcón ◽  
Silvia Irene Boehringer ◽  
Sebastián Sánchez ◽  
...  
Keyword(s):  
Growth Factors ◽  
Fish Species ◽  
Native Fish ◽  
Feeding Period ◽  
Exogenous Feeding ◽  
Growth And Survival ◽  
Piaractus Mesopotamicus ◽  
Egg Incubation ◽  
Probiotic Product

ABSTRACT: Probiotics are a novel alternative to antibiotics as growth factors. Previously, our group isolated, selected and tested in vivo, eight autochthonous strains. They showed no significant effects when administered individually. However, the best doses, stages and ways of administration were combined in a multi strain formula. The aim of this research was to evaluate the effect of this probiotic product on the growth and survival of Piaractus mesopotamicus larvae. The administration was implemented during egg incubation and endogenous feeding period (5), during larvae exogenous feeding period (10) and all along the experiment (15). A group without microorganisms was used as control. The probiotic generates significant increments of mean weight and not significant increases of survival and biomass in two of the three tested stages. These results demonstrate the effectiveness of an autochthonous probiotic formula for the culture of this native fish species.

Safety and Security of Daflon 500 mg in Venous Insufficiency and in Hemorrhoidal Disease

Angiology ◽  
1994 ◽  
Vol 45 (6_part_2) ◽  
pp. 579-584 ◽  
Author(s):  
Olivier C. Meyer
Keyword(s):  
Clinical Trials ◽  
Breast Milk ◽  
Lethal Dose ◽  
Reproductive Function ◽  
The Elderly ◽  
Prolonged Treatment ◽  
Active Principle ◽  
Mutagenic Action ◽  
Long Term Treatment ◽  
One Year

Daflon 500 mg* is a new flavonoid vasoprotector venotonic agent whose active principle is micronized and contains 90% diosmin and 10% flavonoids expressed as hesperidin. In animal studies, the safety of Daflon 500 mg is shown by an LD50 (lethal dose so) of more than 3 g/kg, ie, 180 times the daily therapeutic dose, as well as by the absence of any toxic effect after repeated oral dosing for thirteen and twenty-six weeks, using a dose representing 35 times the daily dosage, in the rate and primate. Daflon 500 mg has no mutagenic action nor any significant effect on reproductive function. Gastrointestinal tolerance is good when administered orally in the rat. Transplacental passage and passage into breast milk are minimal. In the rat, 0.003% of the administered dose has been found in each fetus and 1% in breast milk. Clinical trials fulfill international scientific requirements and have collected more than 2850 patients treated with Daflon 500 mg at the dosage of two tablets per day for six weeks to one year. The proportion of patients with side effects (10% of those treated), essentially of a gastrointestinal or autonomic nature and leading to a rate of only 1.1% trial dropouts, is less than described in 225 patients given a placebo (13.9%) in controlled trials. Satisfactory clinical acceptability already confirmed in the short term was equally found in long-term treatment. Hemodynamic parameters (systolic and diastolic blood pressure) as well as laboratory parameters (hematology, liver and renal function, metabolic) were uninfluenced even by prolonged treatment for one year at the dosage of two tablets per day. No contraindications have been found during the therapeutic use of Daflon 500 mg, even in the elderly and in pregnant women. No evidence has been found of any interference with combined drugs. Daflon 500 mg is free of any photosensitizing action. Daflon 500 mg combines thoroughly proven therapeutic efficacy with excellent safety of use confirmed in specific and methodologically reliable toxicologic studies as well as in a large number of clinical trials with patients treated daily for six weeks to one year.

Sign in / Sign up

Export Citation Format

Share Document