scholarly journals Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue

2005 ◽  
Vol 13 (4) ◽  
pp. 382-386 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Danilo Alanis ◽  
Renato Menezes ◽  
Ariadne Letra ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Repair Process ◽  
Giant Cells ◽  
Tissue Response ◽  
Control Group ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis ◽  
Severe Adverse Reactions

The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.

scholarly journals Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft

2006 ◽  
Vol 17 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Felipe Ladeira Pereira ◽  
Tânia Mary Cestari ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Bone Graft ◽  
Inflammatory Infiltrate ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Test Group ◽  
Tissue Response ◽  
Test Material ◽  
Control Group ◽  
Bovine Bone ◽  
Body Type

The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. Xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. The purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. Forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. Samples were collected after 10, 20, 30 and 60 days and processed histologically. Histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. In later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. In conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.

scholarly journals Biocompatibility evaluation of a new bioresorbable pin for membrane fixation

2010 ◽  
Vol 21 (6) ◽  
pp. 482-490 ◽  
Author(s):  
Tania Mary Cestari ◽  
Rodrigo Cardoso de Oliveira ◽  
Jefferson Tomio Sanada ◽  
Gustavo Pompermaier Garlet ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Plasma Cells ◽  
Immune Reaction ◽  
Subcutaneous Tissue ◽  
Giant Cells ◽  
Collagen Fibers ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Bovine Bone ◽  
Microstructural Aspect ◽  
Alternative Material

The aim of this study was to morphometrically analyze the tissue response to a customized pin obtained from devitalized bovine cortical bone (DBCB-pin) implanted in the subcutaneous tissue of rats, as well as to assess its microstructural aspect by scanning electron microscopy (SEM). The pins were implanted in the subcutaneous tissue of 20 rats, which were killed at 7, 14, 28 and 60 days (5 rats/period) after implantation. In the subcutaneous tissue, DBCB-pin promoted the formation of a fibrous capsule. At 7 days, capsule showed thickness of 70 ± 3.2 µm with higher density of newly formed capillaries and smaller density of collagen fibers. Between 14 and 60 days, more organized fibrous capsule exhibited smaller thickness (53 ± 5.5 µm) with higher density of fibroblasts and collagen fibers. In this period, a small and slow bioresorption of the DBCB-pin by macrophages and rare multinucleated giant cells without tissue damage was observed. The thickness of DBCB-pin resorbed was in mean only of 9.3 µm. During all experimental periods not occurred presence of immune reaction cells as lymphocytes and plasma cells. It was concluded that the pin derived from cortical bovine bone was well tolerated by subcutaneous tissue of rats and slowly resorbed could be an alternative material for membrane fixation in the guided tissue regeneration procedures.

scholarly journals Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

2016 ◽  
Vol 27 (6) ◽  
pp. 657-663 ◽  
Author(s):  
Flávia Emi Razera Baldasso ◽  
Patrícia Maria Poli Kopper ◽  
Renata Dornelles Morgental ◽  
Liviu Steier ◽  
José Antônio Poli de Figueiredo ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Inflammatory Cells ◽  
Giant Cells ◽  
Negative Control ◽  
Tissue Response ◽  
Biological Behavior ◽  
Control Group ◽  
Tissue Samples ◽  
New Formulation ◽  
Post Hoc

Abstract Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

scholarly journals Bovine Onchocerciasis Caused by Onchocerca armillata and O. gutturosa

1978 ◽  
Vol 15 (4) ◽  
pp. 495-505 ◽  
Author(s):  
A. H. Cheema ◽  
B. Ivoghli
Keyword(s):  
Foreign Body ◽  
Connective Tissue ◽  
Plasma Cells ◽  
Giant Cells ◽  
Tissue Reaction ◽  
Foreign Body Reactions ◽  
Gross Lesions ◽  
Acute Changes

Onchocerca armillata was found in 284 (28%) of 1,016 aortas, and O. gutturosa in 82 (28.87%) of 284 nuchal ligaments and in 11 (7.85%) of 140 rumenosplenic areas in specimens from cattle slaughtered during a 12-month period. Adult parasites were not found in 600 hides. Gross lesions included parasitic tunnels, nodules, roughening and calcification in the aortic walls. The connective tissue of nuchal ligaments and rumenosplenic areas was increased in amount, was gelatinous and brown and had a few nodules. Microscopically there were acute changes with oedema, haemorrhages and cellular infiltrations predominantly by eosinophils. There were chronic granulomatous reactions characterized by accumulation of macrophages, lymphocytes, plasma cells, giant cells, calcification and fibrosis around degenerate and dead parasites. Neutral fat was in parasites and surrounding cells. Both changes often occurred in the same specimen and many samples had parasites but no tissue reaction. Hypersensitivity, foreign-body reactions and parasitic toxins apparently were involved in the genesis of these lesions.

scholarly journals The Dynamics of Subcutaneous Tissue Response to Microorganisms Associated with the Extract of Araçá (Psidium cattleianum): An Edemogenic and Microscopic Analysis

2017 ◽  
Vol 20 (2) ◽  
pp. 93 ◽  
Author(s):  
Alessandra Cury Machado ◽  
Denise Belucio Ruviere ◽  
Renata Zoccal Novais ◽  
Carlos Roberto Emerenciano Bueno ◽  
Elerson Gaetti Jardim Jr ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Male Rats ◽  
Saline Control ◽  
Control Group ◽  
Dorsal Region ◽  
Hydroalcoholic Extract ◽  
Psidium Cattleianum ◽  
Significant Difference

<p><strong>Objective:</strong> To evaluate <em>in vivo </em>tissue reaction to the extract of araçá (<em>Psidium cattleianum</em>) associated with inactivated microorganisms. <strong>Material and Methods:</strong> A 0.1 mL suspension was used containing Porphyromonas gingivalis, Prevotella intermedia, <em>Fusobacterium nucleatum, Enterococcus faecalis, Peptostreptococcus micros</em>, and <em>Porphyromonas endodontalis,</em> which were inactivated by heat and mixed into a 1.0 mL saline (control group), an aqueous solution, or a hydroalcoholic extract of araçá. Eighteen male rats (<em>Rattus norvegiccus</em>) under general anesthesia received 0.2 mL of 1% intravenous Evans blue. Thirty minutes later, 0.1 mL of one of the associations was injected into the animals’ dorsal region. The animals were euthanized after 3 and 6 hours, and the materials obtained were placed in formamide for 72 hours then analyzed in a spectrophotometer (λ=630 hm). For the morphological analysis, 30 rats received polyethylene tubes implants with the extracts or the saline with the associations in the dorsal region and euthanized after 7 and 30 days to be analyzed according to an inflammation cell score. <strong>Results:</strong> No significant difference (p&gt;0.05) was observed in the edema among groups. The optical microscopy results showed a repair in the 30-day-period, which was higher when compared to the 7-day-period (p&lt;0.0001). Nevertheless, in the 7-day-period, the hydroalcoholic extract presented a significant response compared to the aqueous extract (p=0.05) and a trend for better results than the control group. <strong>Conclusion: </strong>The aqueous and hydroalcoholic araçá extracts associated with inactivated microorganisms showed similar responses to control, indicating no interference on the toxic effects of the bacterial components in tissue repair.</p><p><strong>Keywords</strong></p><p>Anaerobic bacteria; Edema; Inflammation; Plant extracts; <em>Psidium.</em></p>

scholarly journals Tissue Response after Subcutaneous Implantation of Different Glass Ionomer-Based Cements

2019 ◽  
Vol 30 (6) ◽  
pp. 599-606
Author(s):  
Carolina Maschietto Pucinelli ◽  
Raquel Assed Bezerra da Silva ◽  
Luã Lopes Borges ◽  
Alberto Tadeu do Nascimento Borges ◽  
Paulo Nelson-Filho ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Infiltrate ◽  
Collagen Fiber ◽  
Fiber Formation ◽  
Tissue Response ◽  
Control Group ◽  
Advanced Stage ◽  
Glass Ionomer ◽  
Granulomatous Tissue ◽  
Subcutaneous Connective Tissue

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice after implantation of different glass ionomer-based cements (EQUIA® Forte Fil, EQUIA® Fil and Ketac™ Universal Aplicap™). Eighty-seven isogenic BALB/c mice were allocated in 12 groups, 9 were considered as experimental groups (Ketac, E. Fil and E. Forte at 7, 21 and 63 days) and 3 controls (empty polyethylene tubes at 7, 21 and 63 days). After the experimental periods, the subcutaneous connective tissue surrounding the implanted material was removed and subjected to histotechnical processing and staining with hematoxylin and eosin. A histopathological description of the tissue reaction surrounding each material and a semi-quantitative analysis of collagen fiber formation and inflammatory infiltrate were performed. Additionally, the thickness of the granulomatous tissue in contact with each material was measured. Data were analyzed statistically (α=0.05) by the Kruskal-Wallis test, followed by Dunn post-test. Initially, the collagen fiber formation was not different among all the tested materials (p>0.05) but was different at 21 days with the control group presenting the most advanced stage of collagen fiber formation. At 63 days, EQUIA® Forte Fil group showed the most advanced stage of collagen fiber formation, compared to EQUIA® Fil group (p<0.05). The inflammatory infiltrate was not different among the tested materials in any experimental period (p>0.05). The thickness of the granulomatous tissue was greater in the E. Forte group, compared to control in all periods. All glass ionomer-based cements showed tissue compatibility, according to the evaluated parameters.

scholarly journals A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2869
Author(s):  
Verónica Molina-Hernández ◽  
María T. Ruiz-Campillo ◽  
Francisco J. Martínez-Moreno ◽  
Leandro Buffoni ◽  
Álvaro Martínez-Moreno ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Plasma Cells ◽  
Fasciola Hepatica ◽  
Vaccine Trial ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Control Group ◽  
Infected Control Group ◽  
Hepatic Lesions ◽  
Host Parasite

Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial.

scholarly journals Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1171 ◽  
Author(s):  
Roberto Alameda Hoshino ◽  
Guilherme Ferreira da Silva ◽  
Mateus Machado Delfino ◽  
Juliane Maria Guerreiro-Tanomaru ◽  
Mario Tanomaru-Filho ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Physical Properties ◽  
Enterococcus Faecalis ◽  
Subcutaneous Tissue ◽  
Setting Time ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Group ◽  
Significant Difference

We investigated the physical properties, antimicrobial activity, and tissue reaction to Apexit Plus in comparison to Sealapex. Flow, radiopacity, setting time, and solubility were evaluated in each material. The antimicrobial activity against Enterococcus faecalis was performed. Polyethylene tubes containing Apexit Plus or Sealapex, and without material (control group) were implanted into the subcutaneous tissue of rats. At 7, 15, 30, and 60 days of implantation, the specimens were paraffin-embedded and the number of inflammatory cells (ICs) and the amount of birefringent collagen (BC) were quantified. The von Kossa reaction followed by immunohistochemistry for detection of alkaline phosphatase (ALP) was also performed. Statistical analysis was performed with ANOVA and Tukey test (p ≤ 0.05). The flow value of Apexit Plus was greater than Sealapex, whereas the radiopacity (3.44 mm Al) was lower than Sealapex (6.82 mm Al). Apexit Plus showed lower solubility and shorter initial and final setting (p < 0.0001), whereas the antimicrobial activity was significantly greater than Sealapex. Although the number of ICs was higher in Apexit Plus (p = 0.0009) at 7 days, no significant difference was detected between Apexit Plus and Sealapex at 15, 30, and 60 days. All groups showed higher values for BC in the capsules over time. ALP-immunolabelled cells were observed, mainly around von Kossa-positive structures, either in the capsules of Apexit Plus or Sealapex. Therefore, our results revealed that Apexit Plus exhibited a greater effectiveness against Enterococcus faecalis and better physical properties than Sealapex, except for the radiopacity. In vivo findings indicate that Apexit Plus is biocompatible and presents potential bioactivity in the subcutaneous tissue.

Long-term observation of subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

1997 ◽  
Vol 111 (8) ◽  
pp. 702-706 ◽  
Author(s):  
Dong-Jun Li ◽  
Katsuichiro Ohsaki ◽  
Kunio Ii ◽  
Qing Ye ◽  
Yoko Nobuto ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Cell Types ◽  
Giant Cells ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Histological Characteristics ◽  
Soft Tissue Response ◽  
Long Term Observation ◽  
Different Cell Types

AbstractThe present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram™ discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram™ is useful for reconstructive surgery.

scholarly journals Comparison of Rat Connective Tissue Response to BioMTA, Angelus MTA, and Root MTA

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Marzieh Alimohammadi ◽  
Sina Mirzaee-Rad ◽  
Farideh Feizi ◽  
Hadi Shirzad Juybari ◽  
Akam Saeidi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Wistar Rats ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Control Group ◽  
Capsule Formation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Difference ◽  
Fibrous Capsule Formation

Due to the widespread use of MTA in dentistry and various brands of this product, we decided to compare the three brands available in the country market by their biocompatibility. We divided 20 male Wistar rats into four groups. After local anesthesia and washing, we made two incisions on both sides (4 incisions in total). The experimental groups were Angelus MTA (Angelus, Brazil), BioMTA (CERKAMED, Poland), Root MTA (Dr. Lotfi, Tabriz, Iran), and the control group. The resulting paste was placed in a tube and implanted subcutaneously into male Wistar rats. Wistar rats were sacrificed 7, 15, 30, and 60 days later, with high anesthetic doses. The sample implanted in 10% formalin was stabilized after tissue processing and H&E staining under a microscope. The inflammatory reaction in the tissues received different scores at the beginning of the tube opening. BioMTA had the highest inflammatory response among the groups, but the difference was not statistically significant ( p  > 0.05). Also, there was no significant difference between the groups’ granulation and calcification ( p  < 0.05). There was a significant difference between BioMTA, Angelus MTA, Root MTA, and control groups in fibrous capsule formation ( p  < 0.05). Angelus MTA showed the lowest mean fibrous capsule formation in all periods. The effects of Angelus MTA, Root MTA, and BioMTA on connective tissue were investigated and compared. According to this study, these materials have good biocompatibility. According to the findings and statistical analysis, Angelus MTA has the most biocompatibility.

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