scholarly journals Biocompatibility evaluation of a new bioresorbable pin for membrane fixation

2010 ◽  
Vol 21 (6) ◽  
pp. 482-490 ◽  
Author(s):  
Tania Mary Cestari ◽  
Rodrigo Cardoso de Oliveira ◽  
Jefferson Tomio Sanada ◽  
Gustavo Pompermaier Garlet ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Plasma Cells ◽  
Immune Reaction ◽  
Subcutaneous Tissue ◽  
Giant Cells ◽  
Collagen Fibers ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Bovine Bone ◽  
Microstructural Aspect ◽  
Alternative Material

The aim of this study was to morphometrically analyze the tissue response to a customized pin obtained from devitalized bovine cortical bone (DBCB-pin) implanted in the subcutaneous tissue of rats, as well as to assess its microstructural aspect by scanning electron microscopy (SEM). The pins were implanted in the subcutaneous tissue of 20 rats, which were killed at 7, 14, 28 and 60 days (5 rats/period) after implantation. In the subcutaneous tissue, DBCB-pin promoted the formation of a fibrous capsule. At 7 days, capsule showed thickness of 70 ± 3.2 µm with higher density of newly formed capillaries and smaller density of collagen fibers. Between 14 and 60 days, more organized fibrous capsule exhibited smaller thickness (53 ± 5.5 µm) with higher density of fibroblasts and collagen fibers. In this period, a small and slow bioresorption of the DBCB-pin by macrophages and rare multinucleated giant cells without tissue damage was observed. The thickness of DBCB-pin resorbed was in mean only of 9.3 µm. During all experimental periods not occurred presence of immune reaction cells as lymphocytes and plasma cells. It was concluded that the pin derived from cortical bovine bone was well tolerated by subcutaneous tissue of rats and slowly resorbed could be an alternative material for membrane fixation in the guided tissue regeneration procedures.

scholarly journals Microscopic analisys of porous microgranular bovine anorganic bone implanted in rat subcutaneous tissue

2005 ◽  
Vol 13 (4) ◽  
pp. 382-386 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Danilo Alanis ◽  
Renato Menezes ◽  
Ariadne Letra ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Repair Process ◽  
Giant Cells ◽  
Tissue Response ◽  
Control Group ◽  
Microscopy Analysis ◽  
Light Microscopy Analysis ◽  
Severe Adverse Reactions

The tissue response to porous bovine anorganic bone implanted in rat connective tissue was evaluated by subjective light microscopy analysis. Forty rats were divided into two groups: control (empty collagen capsules) and test (collagen capsule filled with 0.1g biomaterial) and killed 10, 20, 30 and 60 days after implantation. At 10 days, intense chronic inflammatory infiltrate consisting mainly of macrophages and inflammatory multinucleated giant cells (IMGC) was observed. Neutrophils, plasma cells and lymphocytes were present in discrete amounts and slowly disappeared along the repair process. Porosity of the material was filled by reaction connective tissue exhibiting IMGC. The fibrosis was more intense after 60 days and clearly higher than the control group. Thus, the material did not cause any severe adverse reactions and did not stimulate the immune system. Based on the results it could be concluded that deproteinized bovine cancelous bone was well tolerated by rat connective tissue.

scholarly journals Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft

2006 ◽  
Vol 17 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Willian Fernando Zambuzzi ◽  
Rodrigo Cardoso de Oliveira ◽  
Felipe Ladeira Pereira ◽  
Tânia Mary Cestari ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Bone Graft ◽  
Inflammatory Infiltrate ◽  
Plasma Cells ◽  
Subcutaneous Tissue ◽  
Test Group ◽  
Tissue Response ◽  
Test Material ◽  
Control Group ◽  
Bovine Bone ◽  
Body Type

The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. Xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. The purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. Forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. Samples were collected after 10, 20, 30 and 60 days and processed histologically. Histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. In later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. In conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.

Long-term observation of subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

1997 ◽  
Vol 111 (8) ◽  
pp. 702-706 ◽  
Author(s):  
Dong-Jun Li ◽  
Katsuichiro Ohsaki ◽  
Kunio Ii ◽  
Qing Ye ◽  
Yoko Nobuto ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Cell Types ◽  
Giant Cells ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Histological Characteristics ◽  
Soft Tissue Response ◽  
Long Term Observation ◽  
Different Cell Types

AbstractThe present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram™ discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram™ is useful for reconstructive surgery.

THE REACTION OF VARIOUS TISSUES TO IMPLANTS OF A COLLAGEN DERIVATIVE

1949 ◽  
Vol 27e (2) ◽  
pp. 94-104 ◽  
Author(s):  
Arthur F. Battista
Keyword(s):  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Giant Cells ◽  
Foreign Body Response ◽  
Tissue Response ◽  
Histological Structure ◽  
Bovine Bone ◽  
Negative Results ◽  
Antigenic Properties ◽  
Two Stages

This study deals with the histological reaction of subcutaneous connective tissue, muscle, peritoneum, nervous tissue, and bone to implants of a collagen derivative obtained from bovine bone, which has been called Collatissue A. The tissue response was in two stages, first, a fibroblastic proliferation about the implant and second, an infiltration by small and large mononuclear cells. Capillary proliferation occurred around the implant. The material gradually lost its normal histological structure, became amorphous, and was replaced by fibrous tissue. There was no polymorphonuclear cell response and giant cells appeared rarely. Silk, catgut, and tantalum foil were used as control materials. They induced a typical foreign body response, which included polymorphonuclear and giant cells. The antigenic properties of Collatissue A were studied to a limited extent with negative results. Collatissue A has certain physical properties, such as tensile strength and flexibility, that suggest possible surgical applications of this material.

scholarly journals Rat subcutaneous tissue response to MTA Fillapex® and Portland cement

2013 ◽  
Vol 24 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Nádia Carolina Teixeira Marques ◽  
Natalino Lourenço Neto ◽  
Ana Paula Fernandes ◽  
Camila de Oliveira Rodini ◽  
Marco Antônio Hungaro Duarte ◽  
...  
Keyword(s):  
Portland Cement ◽  
Propylene Glycol ◽  
Inflammatory Reaction ◽  
Normal Tissue ◽  
Subcutaneous Tissue ◽  
Collagen Fibers ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Filling Material ◽  
Tissue Healing

The aim of this study was to evaluate the response of rat subcutaneous tissue to MTA Fillapex® (Angelus), an experimental root canal filling material based on Portland cement and propylene glycol (PCPG), and a zinc oxide, eugenol and iodoform (ZOEI) paste. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 7 and 15 days. The specimens were stained with hematoxylin and eosin, and evaluated regarding inflammatory reaction parameters by optical microscopy. The intensity of inflammatory response against the sealers was analyzed by two blinded and previously calibrated examiners for all experimental periods (kappa=0.96). The histological evaluation showed that all materials caused a moderate inflammatory reaction at 7 days, which subsided with time. A greater inflammatory reaction was observed at 7 days in the tubes filled with ZOEI paste. Tubes filled with MTA Fillapex presented some giant cells, macrophages and lymphocytes after 7 days. At 15 days, the presence of fibroblasts and collagen fibers was observed indicating normal tissue healing. The tubes filled with PCPG showed similar results to those observed in MTA Fillapex. At 15 days, the inflammatory reaction was almost absent at the tissue, with several collagen fibers indicating normal tissue healing. Data were analyzed by the nonparametric Kruskal-Wallis test (α=0.05). Statistically significant difference (p<0.05) was found only between PCPG at 15 days and ZOEI at 7 days groups. No significant differences were observed among the other groups/periods (p>0.05). MTA Fillapex and Portland cement added with propylene glycol had greater tissue compatibility than the PCPG paste.

scholarly journals Rat subcutaneous tissue response to modified Portland Cement, a new mineral trioxide aggregate

2009 ◽  
Vol 20 (2) ◽  
pp. 112-117 ◽  
Author(s):  
Ricardo Martínez Lalis ◽  
María Laura Esaín ◽  
Gabriel A. Kokubu ◽  
Julia Willis ◽  
Carolina Chaves ◽  
...  
Keyword(s):  
Foreign Body ◽  
Portland Cement ◽  
Inflammatory Infiltrate ◽  
Subcutaneous Tissue ◽  
Mineral Trioxide Aggregate ◽  
Giant Cells ◽  
Tissue Response ◽  
New Mineral ◽  
Tissue Samples ◽  
Foreign Body Giant Cells

The purpose of this study was compare the biocompatibility of modified Portland cement (CPM) and mineral trioxide aggregate (MTA) in a subcutaneous rat model. Twenty-four male Wistar rats were used. Three silicon tubes were placed on the dorsal subcutaneous tissue of each animal: one tube contained MTA, one tube contained CPM and the other was an empty tube. The rats were sacrificed in 3 groups of 8 animals at 7, 14 and 30 postoperative days, respectively. Tissue samples were fixed in 10% buffered formalin, embedded in paraffin, and serial sections were cut and stained with hematoxylin and eosin, Masson Trichrome and Luna's stain. At day 7, the empty tubes displayed a mild inflammatory infiltrate. In the CPM group, an inflammatory infiltrate was observed with some eosinophils and immature connective tissue. The MTA group showed a similar infiltrate without eosinophils and presence of abundant necrotic tissue and numerous multinucleate foreign body giant cells. At day 14, the chronic infiltrate with eosinophils persisted when in contact with CPM. In the MTA group, necrosis and distant giant cells could still be seen. At day 30, all 3 groups showed mature fibrous collagenous tissue. These findings indicate a different response to the materials evaluated in this study. Although, MTA and CPM induced a chronic inflammatory infiltrate, necrosis and multinucleated foreign body giant cells predominated in the MTA group, while in the CPM group numerous eosinophils were seen at all the observational periods.

Subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

1995 ◽  
Vol 109 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Peng-Cheng Cui ◽  
Katsuichiro Ohsaki ◽  
Kunio Ii ◽  
Satoru Tenshin ◽  
Terushige Kawata
Keyword(s):  
Inflammatory Reaction ◽  
Subcutaneous Tissue ◽  
Thick Layer ◽  
Cell Types ◽  
Giant Cells ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Sham Operation ◽  
Soft Tissue Response ◽  
Different Cell Types

AbstractA study was carried out in order to obtain further information about the soft tissue response to thin Apaceram® discs of dense hydroxyapatite (HA) implanted in rats for various periods of time between one day and 10 months. The Apaceram® discs were implanted subcutaneously into the interscapular region of 33 rats. A sham operation was performed on eight rats used as controls. Decalcified histological sections stained with haematoxylin and eosin and Mallory's azan were examined and the different cell types found around the implants were counted. It was found that an acute inflammatory reaction occurred after one day and disappeared at about two weeks after implantation. In the test groups, macrophages and lymphocytes disappeared about one week later, and no inflammatory reaction was observed from one to three months. However, a tissue reaction occurred at six months with the appearance of macrophages and lymphocytes, and decreased gradually at 10 months. Meanwhile, a few foreign body giant cells at the Apaceram®-tissue interface and a thick layer of fibrous connective tissue around the Apaceram® disc were observed at 10 months. No osteogenesis was observed in any specimen. The results obtained so far suggest that Apaceram® is still a useful material for reconstructive surgery, despite the possible appearance of a slight macrophage reaction at six months.

scholarly journals Biocompatibility nanostructured biomaterials based on calcium aluminate

2018 ◽  
Vol 146 (11-12) ◽  
pp. 634-640
Author(s):  
Ognjenka Jankovic ◽  
Smiljana Paras ◽  
Ljiljana Tadic-Latinovic ◽  
Renata Josipovic ◽  
Vukoman Jokanovic ◽  
...  
Keyword(s):  
Calcium Aluminate ◽  
Subcutaneous Tissue ◽  
Mineral Trioxide Aggregate ◽  
Nanostructured Material ◽  
Tissue Response ◽  
Fibrous Capsule ◽  
Control Group ◽  
Vascular Congestion ◽  
Significant Difference ◽  
The Difference

Introduction/Objective. The aim of this paper was to verify the biocompatibility of the newly synthesized nanostructured material based on calcium aluminate after implantation into the subcutaneous tissue of rats. Methods. The study included 18 rats aged 10?11 weeks, divided into two experimental groups (n = 9). In all animals, incision took place on the back and two pockets of 15 mm in depth were made, in which sterile polyethylene tubes with test materials [calcium aluminate cement (ALBO-CA), calcium silicate cement with the addition of hydroxyapatite (ALBO-CSHA), and mineral trioxide aggregate (MTA) for the control group) were implanted. Six rats of each group were sacrificed in three observational periods (seven, 15, 30 days). Pathohistological analysis included inflammation, bleeding, fibrous capsule, and tissue integrity around the implanted material. Results. After seven days of treatment, ALBO-CA and ALBO-CSHA showed better tissue response compared to MTA, with a statistically significant difference in inflammation intensity (p = 0.2781). The difference in vascular congestion and thickness of the fibrous capsule after implantation of ALBO-CA material compared to MTA was also statistically significant (p = 0.5567). At the end of the 30-day evaluation period, an identical inflammatory response of connective tissue at the site of implanting ALBO-CA, ALBO-CSHA, and MTA (score of 0.7) was recorded. The formation of thick or moderately thick fibrous capsule was found to be the thickest in ALBO-CA (grade 3.7). There were no statistically significant differences between the parameters analyzed after 30 days. Conclusion. Newly synthesized ALBO-CA showed a satisfactory tissue response and confirmed biocompatibility after implantation in subcutaneous tissue of rats.

scholarly journals Tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats

2004 ◽  
Vol 15 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Rodrigo Cardoso de Oliveira ◽  
Renato Menezes ◽  
Tânia Mary Cestari ◽  
Eulázio Mikio Taga ◽  
Rumio Taga ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Mononuclear Cells ◽  
Subcutaneous Tissue ◽  
Periodontal Regeneration ◽  
Bone Substitutes ◽  
Giant Cells ◽  
Bone Defects ◽  
Tissue Response ◽  
Junctional Epithelium ◽  
Periodontal Bone Defects

The treatment of persistent bone defects has encouraged the search for proper techniques or bone substitutes. In Dentistry, a common problem in the treatment of periodontal bone defects is the growth of tissues within the lesion, such as the junctional epithelium, which impair regeneration of these tissues. Guided tissue regeneration (GTR), based on the separation of the tissues by means of membranes or barriers, was developed in an attempt to improve periodontal regeneration. The aim of this study was to histologically evaluate the tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats. The study periods were 1, 3, 7, 15, 30 and 60 days after implantation. Analysis of the histological sections demonstrated a moderate to intense inflammatory response at 1 and 3 days, moderate at 7 and 15 days, and almost absent at 30 and 60 days. Resorption of the membrane began 15 days after implantation, and at 60 days only remnants could be detected in some animals. We concluded that the demineralized bovine cortical bone membrane was well tolerated by the tissues and is completely resorbed after 30-60 days by mononuclear cells and multinucleated giant cells, which disappear upon completion of the process.

scholarly journals Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

2016 ◽  
Vol 27 (6) ◽  
pp. 657-663 ◽  
Author(s):  
Flávia Emi Razera Baldasso ◽  
Patrícia Maria Poli Kopper ◽  
Renata Dornelles Morgental ◽  
Liviu Steier ◽  
José Antônio Poli de Figueiredo ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Inflammatory Cells ◽  
Giant Cells ◽  
Negative Control ◽  
Tissue Response ◽  
Biological Behavior ◽  
Control Group ◽  
Tissue Samples ◽  
New Formulation ◽  
Post Hoc

Abstract Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

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