scholarly journals Synergistic effect of acetyl salicylic acid and DL-Beta-aminobutyric acid on biocontrol efficacy of Bacillus strains against tomato bacterial wilt

2013 ◽  
Vol 38 (2) ◽  
pp. 102-113 ◽  
Author(s):  
Abdulwareth Abdlkader Almoneafy ◽  
Mohammad Reza Ojaghian ◽  
Xu Seng-fu ◽  
Muhammad Ibrahim ◽  
Xie Guan-Lin ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Synergistic Effect ◽  
Bacterial Wilt ◽  
Aminobutyric Acid ◽  
Acetyl Salicylic Acid ◽  
Biocontrol Efficacy ◽  
Tomato Bacterial Wilt ◽  
Bacillus Strains

scholarly journals The impact of Paenibacillus polymyxa HY96-2 luxS on biofilm formation and control of tomato bacterial wilt

2019 ◽  
Vol 103 (23-24) ◽  
pp. 9643-9657 ◽  
Author(s):  
Jincui Yi ◽  
Daojing Zhang ◽  
Yuejuan Cheng ◽  
Jingjing Tan ◽  
Yuanchan Luo
Keyword(s):  
Bacterial Wilt ◽  
Late Stage ◽  
Biofilm Formation ◽  
Early Stage ◽  
Regulatory Gene ◽  
Paenibacillus Polymyxa ◽  
Deletion Strain ◽  
Biocontrol Efficacy ◽  
The Impact ◽  
Tomato Bacterial Wilt

Abstract The focus of this study was to investigate the effects of luxS, a key regulatory gene of the autoinducer-2 (AI-2) quorum sensing (QS) system, on the biofilm formation and biocontrol efficacy against Ralstonia solanacearum by Paenibacillus polymyxa HY96-2. luxS mutants were constructed and assayed for biofilm formation of the wild-type (WT) strain and luxS mutants of P. polymyxa HY96-2 in vitro and in vivo. The results showed that luxS positively regulated the biofilm formation of HY96-2. Greenhouse experiments of tomato bacterial wilt found that from the early stage to late stage postinoculation, the biocontrol efficacy of the luxS deletion strain was the lowest with 50.70 ± 1.39% in the late stage. However, the luxS overexpression strain had the highest biocontrol efficacy with 75.66 ± 1.94% in the late stage. The complementation of luxS could restore the biocontrol efficacy of the luxS deletion strain with 69.84 ± 1.09% in the late stage, which was higher than that of the WT strain with 65.94 ± 2.73%. Therefore, we deduced that luxS could promote the biofilm formation of P. polymyxa HY96-2 and further promoted its biocontrol efficacy against R. solanacearum.

scholarly journals A GC-ECD Method for Estimation of Free and Bound Amino Acids, γ-Aminobutyric Acid, Salicylic Acid, and Acetyl Salicylic Acid from Solanum lycopersicum (L.)

2011 ◽  
Vol 94 (1) ◽  
pp. 232-242 ◽  
Author(s):  
Hari Charan Meher ◽  
Vijay T Gajbhiye ◽  
Ghanendra Singh
Keyword(s):  
Amino Acids ◽  
Salicylic Acid ◽  
Solanum Lycopersicum ◽  
Iodoacetic Acid ◽  
Operating Conditions ◽  
Aminobutyric Acid ◽  
Acetyl Salicylic Acid ◽  
Electron Capture Detection ◽  
Acid Vapor ◽  
Solanum Lycopersicum L

Abstract A gas chromatograph with electron capture detection method for estimation of selected metabolites—amino acids (free and bound), γ-aminobutyric acid (GABA), salicylic acid (SA), and acetyl salicylic acid (ASA) from tomato—is reported. The method is based on nitrophenylation of the metabolites by 1-fluoro-2,4-dinitrobenzene under aqueous alkaline conditions to form dinitrophenyl derivatives. The derivatives were stable under the operating conditions of GC. Analysis of bound amino acids comprised perchloric acid precipitation of protein, alkylation (carboxymethylation) with iodoacetic acid, vapor-phase hydrolysis, and derivatization with 1-fluoro-2,4-dinitrobenzene in that order. The metabolites were resolved in 35 min, using a temperature-programmed run. The method is rapid, sensitive, and precise. It easily measured the typical amino acids (aspartate, asparagine, glutamate, glutamine, alanine, leucine, lysine, and phenylalanine) used for identification and quantification of a protein, resolved amino acids of the same mass (leucine and isoleucine), satisfactorily measured sulfur amino acids (methionine, cystine, and cysteine), and quantified GABA, SA, and ASA, as well. The developed method was validated for specificity, linearity, and precision. It has been applied and recommended for estimation of 25 metabolites from Solanum lycopersicum (L.).

scholarly journals Effects of Combined Application of Potassium Silicate and Salicylic Acid on the Defense Response of Hydroponically Grown Tomato Plants to Ralstonia solanacearum Infection

2021 ◽  
Vol 13 (7) ◽  
pp. 3750
Author(s):  
Ni-Hao Jiang ◽  
Shi-Han Zhang
Keyword(s):  
Salicylic Acid ◽  
Combination Treatment ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Combined Effects ◽  
Combined Treatments ◽  
Tomato Plants ◽  
Combined Application ◽  
Infected Tomato ◽  
Tomato Bacterial Wilt

Bacterial wilt, caused by soilborne pathogenic bacterium Ralstonia solanacearum, is a serious and widespread disease that affects global tomato production. Both silicon (Si) and salicylic acid (SA) play important roles in enhancing tomato resistance against bacterial wilt, however, their combined effects on the defense responses of infected tomato plants remain unknown. Hence, the combined effects of Si and SA on physiological and biochemical parameters of R. solanacearum-infected tomato plants were investigated. The combination treatment of Si and SA significantly decreased disease incidences, lipoxygenase (LOX) activity and ethylene (ET) production. The combined treatments were more prominent in improving the morphological traits of root systems, such as root length, root surface area, average root diameter and root volume. The activities of polyphenol oxidase (PPO) and peroxidase (POD) and the concentrations of total soluble phenolics (TSPs) and lignin-thioglycolic acid (LTGA) derivatives were significantly increased in the plants with combined treatments. Si in combination with SA could significantly enhance neutral invertase (NI) and acid invertases (AI) activities in the leaves of tomato plants at 3 days post-infection (dpi) compared with application of Si alone. Three defense-related genes, PAL, POD and pathogenesis-related protein 1 (PR1), were significantly induced in Si+SA treatment at 7 dpi when compared with individual application of Si or SA. The expression level of salicylic acid-binding protein 2 (SABP2) was significantly higher for combination treatment when compared with treatment of Si or SA alone. The possible mechanisms involved in the synergistic effects of Si and SA on the control of tomato bacterial wilt were proposed. This study indicates that under hypertonic conditions, the combined application of 2.0 mM potassium silicate (K2SiO3) and 0.5 mM SA had a synergistic effect on the control of tomato bacterial wilt.

scholarly journals Synergistic Effect between Trichoderma virens and Bacillus velezensis on the Control of Tomato Bacterial Wilt Disease

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 439
Author(s):  
You Zhou ◽  
Laying Yang ◽  
Jun Wang ◽  
Lijia Guo ◽  
Junsheng Huang
Keyword(s):  
Treatment Group ◽  
Synergistic Effect ◽  
Bacterial Wilt ◽  
Enzyme Activities ◽  
Disease Incidence ◽  
Wilt Disease ◽  
Trichoderma Virens ◽  
Bacillus Velezensis ◽  
Bacterial Wilt Disease ◽  
Tomato Bacterial Wilt

(1) Background: Ralstonia solanacearum causes tomato bacterial wilt disease, one of the most serious tomato diseases. As the combination of Trichoderma virens (Tvien6) and Bacillus velezensis (X5) was more effective at controlling tomato bacterial wilt disease than a single agent, we investigated the synergistic effect of Tvien6 and X5 in controlling this disease; (2) Methods: The disease incidence, plant heights and weights, relative chlorophyll content (SPAD values), defensive enzymes (PPO, POD, and SOD) activities, and metabolome were estimated among four treatment groups (BR treatment, X5 + R. solanacearum (RS-15); TR treatment, Tvien6+ RS-15; TBR treatment, Tvien6 + X5 + RS-15; and R treatment, RS-15); (3) Results: The R treatment group had the highest disease incidence and lowest plant heights, plant weights, SPAD values, defensive enzyme activities, and D-fructose and D-glucose contents; the TBR treatment group had the lowest disease incidence and highest plant heights, plant weights, SPAD values, defensive enzyme activities, and D-fructose and D-glucose contents; (4) Conclusions: The results revealed that Tvien6 and X5 can both individually promote tomato plant growth, increase leaf chlorophyll content, enhance defensive enzyme activities, and induce the accumulation of D-fructose and D-glucose; however, they were more effective when combined.

Sign in / Sign up

Export Citation Format

Share Document