scholarly journals Perspectives for the use of plant extracts to control the cattle tick Rhipicephalus (Boophilus) microplus

2011 ◽  
Vol 20 (2) ◽  
pp. 89-96 ◽  
Author(s):  
Lígia Miranda Ferreira Borges ◽  
Lorena Alessandra Dias de Sousa ◽  
Carolina da Silva Barbosa
Keyword(s):  
Plant Extracts ◽  
Food Contamination ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Intrinsic Factors ◽  
Scientific Investigations ◽  
Development Of Resistance ◽  
Chemical Composition Of Plants ◽  
Evolution Of Resistance

The evolution of resistance of Rhipicephalus (Boophilus) microplus to synthetic acaricides has given rise to the need for new scientific investigations on alternative ways to control this tick. In this regard, various studies on plants have been developed in an attempt to find extracts with acaricidal properties. Evaluations on plant extracts for controlling R. (B.) microplus have grown intensely over the last decade. There are many advantages from using plant extracts: for example, they can be used in organic cattle farming or even replace synthetic acaricides and they are associated with lower environmental and food contamination, slower development of resistance and lower toxicity to animals and humans. In vitro studies on plant extracts have shown promising results, but most of these extracts have not been tested on animals to validate their use. Difficulties in preparing proper formulations, differences in the chemical composition of plants of the same species due to extrinsic and intrinsic factors and sparse information on active acaricide compounds are hindrances that need to be addressed in order to enable progress within this scientific field.

scholarly journals In vitro activity of the hydroalcoholic extract of Chenopodium ambrosioides against engorged females of Rhipicephalus (Boophilus) microplus

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Eduardo Oliveira ◽  
Manoela da Silva ◽  
Lew Sprenger ◽  
Daniela Pedrassani
Keyword(s):  
High Efficiency ◽  
Boophilus Microplus ◽  
Vitro Activity ◽  
Economic Losses ◽  
Cattle Tick ◽  
Distilled Water ◽  
In Vitro Activity ◽  
Chenopodium Ambrosioides ◽  
Cattle Ticks

ABSTRACT: Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.

scholarly journals Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

2011 ◽  
Vol 20 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Rodrigo Casquero Cunha ◽  
Renato Andreotti ◽  
Fábio Pereira Leivas Leite
Keyword(s):  
Pichia Pastoris ◽  
Boophilus Microplus ◽  
Economic Losses ◽  
Cattle Tick ◽  
High Antibody ◽  
Western Blot Assay ◽  
Development Of Resistance ◽  
Cattle Ticks ◽  
Antibody Titers

The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.

Resistance by cattle to cattle tick, Boophilus microplus. III. The development of resistance to experimental infestations by purebred Sahiwal and Australian Illawarra Shorthorn cattle

1971 ◽  
Vol 22 (2) ◽  
pp. 331 ◽  
Author(s):  
RW Hewetson
Keyword(s):  
Adult Female ◽  
Acquired Resistance ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Control Group ◽  
The Third ◽  
Development Of Resistance ◽  
Significant Difference ◽  
Sahiwal Cattle ◽  
Males And Females

Purebred Sahiwal cattle acquired resistance to Boophilus microplus in a similar manner to crossbred Sahiwal cattle as previously reported. The purebred entire males and females were more resistant than a control group of crossbred steers because they dropped significantly fewer replete ticks than the crossbreds at the third and fourth, but not at the first and second infestations. Adult female ticks dropped by purebred animals were significantly lighter than those dropped by crossbred animals at the fourth infestation only. There was no significant difference in the numbers of eggs laid and hatched from ticks dropped by purebred and crossbred animals. Australian Illawarra Shorthorn steers developed resistance at a second and third infestation which waned at a fourth infestation, but individual steers displayed developing resistance throughout the four infestations. Day of modal drop of replete female ticks appeared to be affected by breed, and developing resistance or season.

In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina

2020 ◽  
Vol 82 (3) ◽  
pp. 397-403
Author(s):  
Jorgelina Torrents ◽  
Nicolás Morel ◽  
Maria V. Rossner ◽  
Norberto C. Martínez ◽  
José R. Toffaletti ◽  
...  
Keyword(s):  
Boophilus Microplus ◽  
Cattle Tick ◽  
In Vitro Diagnosis

A new in vitro test to evaluate the resistance level against acaricides of the cattle tick, Rhipicephalus (Boophilus) microplus

2011 ◽  
Vol 182 (2-4) ◽  
pp. 269-280 ◽  
Author(s):  
L. Lovis ◽  
J.-L. Perret ◽  
J. Bouvier ◽  
J.-M. Fellay ◽  
R. Kaminsky ◽  
...  
Keyword(s):  
Boophilus Microplus ◽  
Cattle Tick ◽  
In Vitro Test ◽  
Resistance Level ◽  
Vitro Test

In vitro and in vivo acaricide action of juvenoid analogs produced from the chemical modification of Cymbopogon spp. and Corymbia citriodora essential oil on the cattle tick Rhipicephalus (Boophilus) microplus

2014 ◽  
Vol 205 (1-2) ◽  
pp. 277-284 ◽  
Author(s):  
Ana Carolina S. Chagas ◽  
Luciana F. Domingues ◽  
Rafaela R. Fantatto ◽  
Rodrigo Giglioti ◽  
Márcia C.S. Oliveira ◽  
...  
Keyword(s):  
Essential Oil ◽  
Chemical Modification ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Corymbia Citriodora

Boophilus microplus: the effect of histamine on the attachment of cattle-tick larvae–studies in vivo and in vitro

Parasitology ◽  
1980 ◽  
Vol 80 (3) ◽  
pp. 487-496 ◽  
Author(s):  
D. H. Kemp ◽  
A. Bourne
Keyword(s):  
Prostaglandin E2 ◽  
Host Resistance ◽  
Host Response ◽  
Attachment Site ◽  
Boophilus Microplus ◽  
Circumstantial Evidence ◽  
Cattle Tick ◽  
Larval Behaviour

SummaryCircumstantial evidence suggests that the earlier detachment of Boophilus microplus larvae from highly resistant cattle follows the release of histamine at the attachment site. In vivo and in vitro experiments show that a proportion of the larvae will detach following injection or infusion of histamine. Other mediators such as bradykinin, prostaglandin E2, 5-hydroxytryptamine and dopamine have little or no effect on tick behaviour in vivo. Sensitivity to histamine declines as larval attachments stabilize, and repeated injections have no effect on the weight of larvae after 3 days on the host. Response to histamine is discussed in relation to host resistance, histology of the feeding lesion and larval behaviour.

The inheritance of DDT-resistance in the cattle tick, Boophilus microplus

1962 ◽  
Vol 13 (5) ◽  
pp. 984 ◽  
Author(s):  
BF Stone
Keyword(s):  
Resistant Strain ◽  
Reference Strain ◽  
Boophilus Microplus ◽  
Autosomal Gene ◽  
Cattle Tick ◽  
Adult Females ◽  
Ddt Resistance ◽  
High Level ◽  
Multiresistant Strain

Adults of a DDT-resistant strain of the cattle tick from central Queensland were crossed with adults of a susceptible reference strain, by means of cardboard mating boxes glued to the skins of cattle. F1, backcross, and F2 larvae were tested for resistance to DDT by enclosure of larvae in filter paper packets impregnated with oil solutions of pp'-DDT. F1 and backcross engorged adult females were tested for resistance by injection with oil solutions of pp'-DDT. There was no evidence of departure from a 1 : 1 ratio in the backcrosses or from a 1 : 2 : 1 ratio in the F2, and there was little difference between the compositions of the F1 reciprocal crosses or among the backcrosses derived from them. Therefore DDT resistance in this strain was considered to be due to a single, incompletely recessive, autosomal gene. Engorged nymphs of the resistant strain moulted later in vitro than nymphs of the susceptible strain, and resistant engorged adult females detached from the host later than susceptible engorged adult females. After 13 generations of DDT-free culturing of a multiresistant strain, the percentage of homozygous DDT-resistant ticks in the strain had fallen from a high level to about 55%. This figure remained constant for a further 10 generations.

scholarly journals In vitro efficacy of plant extracts and synthesized substances on Rhipicephalus (Boophilus) Microplus (Acari: Ixodidae)

2011 ◽  
Vol 110 (1) ◽  
pp. 295-303 ◽  
Author(s):  
Ana Carolina de Souza Chagas ◽  
Luiz Daniel de Barros ◽  
Fernando Cotinguiba ◽  
Maysa Furlan ◽  
Rodrigo Giglioti ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Boophilus Microplus

scholarly journals Efficiency of tick biotherapic on the control of infestation by Rhipicephalus (Boophilus) microplus in Dutch dairy cows.

2021 ◽  
Vol 9 (33) ◽  
pp. 156-164
Author(s):  
Zilda Cristiani Gazim ◽  
Fabiana Borges Padilha Ferreira ◽  
Aristeu Vieira Da Silva ◽  
Kelly Cristina Bolognese ◽  
Ewerton Merlin ◽  
...  
Keyword(s):  
Boophilus Microplus ◽  
Mineral Salt ◽  
Cattle Tick ◽  
Reproductive Efficiency ◽  
In Vitro Tests ◽  
Egg Mass ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Significant Difference

Background: cattle tick Rhipicephalus (Boophilus) microplus poses serious problems for farmers in Brazil, especially because the parasite easily develops resistance to pesticide agents. For this reason, together with other factors including environmental, human and animal contamination and costs, alternative approaches have been sought for. Aims: this study sough to evaluate the efficiency of a tick biotherapic on tick-infested cows. Methods: 34 dairy Dutch cows were divided in 2 groups: one group received 100g/day of mineral salt supplement impregnated with tick biotherapic 12cH for 6 months, and then in alternate days with tick biotherapic 30cH to complete 28 months of treatment; the other group (control) received only the mineral salt supplement. After 28 months of treatment, engorged Rhipicephalus (boophilus) microplus females were collected in both groups, counted and weighed; in vitro tests were carried out to assess mass of ticks; egg mass; egg-hatching rate; and reproductive efficiency. Results: There was significant difference between both groups for all parameters evaluated; tick-mass (p = 0.0008); egg mass (p=0.0044); egg-hatching rate (p= 0.0017); and reproductive efficiency (p = 0.0044). Conclusion: treatment with tick biotherapic significantly decreased the mass of engorged females, deposition and hatching rate of eggs, resulting consequently in the decrease of the reproductive efficiency of ticks.

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