scholarly journals Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1643
Author(s):  
Baptiste Defaye ◽  
Sara Moutailler ◽  
Christian Pietri ◽  
Clemence Galon ◽  
Sébastien Grech-Angelini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Animal Species ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Animal Population ◽  
Blood Samples ◽  
Wild Boars ◽  
On This Island ◽  
First Time ◽  
Babesia Spp

Corsica is the main French island in the Mediterranean Sea and has high levels of human and animal population movement. Among the local animal species, the wild boar is highly prevalent in the Corsican landscape and in the island’s traditions. Wild boars are the most commonly hunted animals on this island, and can be responsible for the transmission and circulation of pathogens and their vectors. In this study, wild boar samples and ticks were collected in 17 municipalities near wetlands on the Corsican coast. A total of 158 hunted wild boars were sampled (523 samples). Of these samples, 113 were ticks: 96.4% were Dermacentor marginatus, and the remainder were Hyalomma marginatum, Hyalomma scupense and Rhipicephalus sanguineus s.l. Of the wild boar samples, only three blood samples were found to be positive for Babesia spp. Of the tick samples, 90 were found to be positive for tick-borne pathogens (rickettsial species). These results confirm the importance of the wild boar as a host for ticks carrying diseases such as rickettsiosis near wetlands and recreational sites. Our findings also show that the wild boar is a potential carrier of babesiosis in Corsica, a pathogen detected for the first time in wild boars on the island.

scholarly journals Blood parasites in dogs from the Belgrade area in the period 2014-2015

2017 ◽  
pp. 69-78
Author(s):  
Ivan Pavlovic ◽  
Ivana Kovacevic ◽  
Radoje Doder ◽  
Branislav Rangelov ◽  
Ivana Nikic ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Rhipicephalus Sanguineus ◽  
Blood Parasites ◽  
Ixodes Persulcatus ◽  
Blood Samples ◽  
Test Kit ◽  
Blood Smears ◽  
Shelter Dogs ◽  
First Time ◽  
Babesia Spp

Prevalence of blood parasites in dogs in the Belgrade area has been investigated continuously during the last 20 years, especially in clinically suspicious dogs. In the period from 2014 to 2015, 249 blood samples of pets (182) and shelter dogs (67) were examined. Using Giemsa-stained blood smears, the presence of Babesia spp. was examined in erythrocytes and the presence of morulae of Ehrlichia spp. and Anaplasma spp. in circu?lating monocytes and granulocytes. To confirm positive findings of ehrlichiosis and ana?plasmosis in blood smears, CaniV-4 Test Kit or IDEXX SNAP 4DX test was used. Infection with two pathogens was found in 78/249 (31.32%) cases; in all cases, the infection with one of the protozoa or bacteria was in combination with heartworms. In blood-smears, babesiosis was found in 39.75% of pet dogs and in 71.64% of shelter dogs, ehrlichiosis in 15.93% and 28.35%, and anaplasmosis in 6.04% and 19.40%, respectivelly. From colected ticks, relative abundance analysis revealed that the species Ixodes ricinus was absolutely dominant and found in 50.53% (47/93), followed by Rhipicephalus sanguineus ? 38.70% (36/93), Derma?centor marginatus ? 9.67% (9/93), D. reticulatus, and Ixodes persulcatus found in 3.22% (3/93), which for the first time occurred in dogs in the Belgrade area and in Serbia.

Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Handan Cetinkaya ◽  
Damla Haktanir ◽  
Seckin Arun ◽  
Cem Vurusaner
Keyword(s):  
Molecular Detection ◽  
Fragment Length Polymorphism ◽  
Common Species ◽  
Mycoplasma Species ◽  
Pcr Assay ◽  
Blood Samples ◽  
Total Prevalence ◽  
Polymerase Chain ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

AbstractThe aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.

scholarly journals Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Mariana Granziera Spolidorio ◽  
Mariana de Medeiros Torres ◽  
Wilma Neres da Silva Campos ◽  
Andréia Lima Tomé Melo ◽  
Michelle Igarashi ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Domestic Dogs ◽  
Hepatozoon Canis ◽  
Babesia Canis ◽  
Mato Grosso ◽  
First Time ◽  
The City ◽  
Babesia Spp

The objective of this study was to report for the first time infection by Hepatozoon spp. and Babesia spp. in 10 dogs from the city of Cuiabá, State of Mato Grosso, central-western Brazil. A pair of primers that amplifies a 574 bp fragment of the 18S rRNA of Hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18S rRNA for Babesia spp. were used. Six dogs were positive for Babesia spp., and 9 were positive for Hepatozoon spp. Co-infection of Babesia spp. and Hepatozoon spp. was seen in 5 dogs. Sequenced samples revealed 100% identity with B. canis vogeli, and H. canis. This is the first molecular detection of H. canis in domestic dogs from Cuiabá. Additionally, it is described for the first time the presence of B. canis vogeli circulating among dogs in Cuiabá.

scholarly journals Molecular detection of bovine hemotrophic mycoplasmas in Mexico

2020 ◽  
Vol 13 (52) ◽  
pp. 67-82
Author(s):  
Rosa Estela Quiroz Castañeda ◽  
Kytzya Mejía Aragón ◽  
Hugo Aguilar Diaz ◽  
Jesús Francisco Preciado de la Torre
Keyword(s):  
Molecular Detection ◽  
Detection Method ◽  
Animal Health ◽  
Clinical Signs ◽  
Health Condition ◽  
Duplex Pcr ◽  
Blood Samples ◽  
End Point ◽  
Mycoplasma Wenyonii ◽  
First Time

The presence of hemoplasmas Candidatus Mycoplasma haemobos and Mycoplasma wenyonii that infect bovine cattle has been reported during the last years. Hemoplasmas may affect animal health either alone or in coinfections with other microorganisms, resulting in anemia and other clinical signs. In Mexico, only Ca. M. haemobos has been detected in cattle; in this work, we report for the first time in our country the presence of M. wenyonii in animals from different geographical sources amd we detected both hemoplasmas by duplex PCR. Also, by single end-point PCR, we found Ca. M. haemobos and M. wenyonii in 96% and 96.29% of the blood samples, respectively. Both hemoplasmas were detected in 50% of the samples analyzed, which suggest that the duplex PCR developed in this work might improve if some modifications are performed. This molecular detection method will provide valuable information to know the health condition of national cattle to prevent pathogen dispersion.

scholarly journals Molecular epidemiology of Leptospira spp. among wild mammals and a dog in Amami Oshima Island, Japan

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249987
Author(s):  
So Shinya ◽  
Yukinori Muraoka ◽  
Daigo Negishi ◽  
Nobuo Koizumi
Keyword(s):  
Wild Boar ◽  
Housekeeping Genes ◽  
High Rate ◽  
Wild Animals ◽  
Nested Polymerase Chain Reaction ◽  
Wild Mammals ◽  
Wild Boars ◽  
Black Rat ◽  
On This Island ◽  
Black Rats

Leptospirosis is a worldwide zoonosis caused by the pathogenic Leptospira spp. Canine and human leptospirosis sometimes occur on Amami Oshima Island, located in the Nansei Archipelago, southwestern Japan; however, information on the causative Leptospira spp. on this island is quite limited. This study aimed to investigate the molecular and serological characteristics of Leptospira spp. isolated from wild animals and a dog in Amami Oshima Island. We obtained seven Leptospira strains by culturing kidney tissues of wild animals, such as black rats (2), wild boars (3), and rabbit (1) as well as blood from a symptomatic dog. Using flaB sequencing and microscopic agglutination test with antisera for 18 serovars, the isolates were identified as Leptospira borgpetersenii serogroups Javanica (black rat), L. interrogans serogroup Australis (black rat and dog), and L. interrogans serogroup Hebdomadis (wild boar and rabbit). The sequence type (ST) of L. borgpetersenii serogroup Javanica was determined to be ST143 via multilocus sequence typing (MLST) using seven housekeeping genes. For L. interrogans, MLST and multiple-locus variable-tandem repeat analysis (MLVA) revealed identical ST and MLVA types in rat and canine isolates, whereas two STs and MLVA types were identified in wild boar isolates. The STs and MLVA types of rabbit and one of the wild boars were identical. Bacterial culture and flaB-nested polymerase chain reaction demonstrated a high rate of Leptospira infection in wild boars (58.3%, 7/12), whereas Leptospira spp. were detected in 4.8% of black rats (2/42). This study revealed diverse Leptospira genotype and serotype maintenance in wild mammals on Amami Oshima Island. MLST and MLVA indicated that black rats were a source of canine infection. Wild boars carry L. interrogans and are considered an important maintenance host because antibodies against serogroup Hebdomadis were detected in human and canine leptospirosis patients on this island.

scholarly journals Survey of Ehrlichia canis, Babesia spp. and Hepatozoon spp. in dogs from a semiarid region of Brazil

2015 ◽  
Vol 24 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Tereza Emmanuelle de Farias Rotondano ◽  
Herta Karyanne Araújo Almeida ◽  
Felipe da Silva Krawczak ◽  
Vanessa Lira Santana ◽  
Ivana Fernandes Vidal ◽  
...  
Keyword(s):  
Blood Smear ◽  
Rhipicephalus Sanguineus ◽  
Immunofluorescence Assay ◽  
Ehrlichia Canis ◽  
Northeastern Brazil ◽  
Semiarid Region ◽  
Blood Samples ◽  
Babesia Vogeli ◽  
Blood Smear Examination ◽  
Babesia Spp

This study assessed the occurrence of Ehrlichia spp., Babesia spp. and Hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the State of Paraíba, Northeastern Brazil. Blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. Blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. The 1,151 ticks collected were identified as Rhipicephalus sanguineus; direct smears revealed E. canis-like morulae in the monocytes of 4% (4/100) of the non-vaccinated female dogs, and 34% and 25% of the dogs tested positive for Ehrlichia canis by indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR), respectively. Blood smear examination revealed Babesia-suggestive merozoites in the erythrocytes of 2% (2/100) of the animals. Babesia vogeli was detected by PCR in ten animals (10%) and was correlated with young age (p = 0.007) and thrombocytopenia (p = 0.01). None of the animals showed Hepatozoon spp. positivity. These results indicate that E. canis is the main tick-borne canine pathogen in the study area and provide the first report of B. vogeli infection in dogs from Paraiba State.

scholarly journals Detection of rabies antibodies in wild boars in north-east Romania by a rabies ELISA test

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Mihaela Anca Dascalu ◽  
Marine Wasniewski ◽  
Evelyne Picard-Meyer ◽  
Alexandre Servat ◽  
Florentina Daraban Bocaneti ◽  
...  
Keyword(s):  
Wild Boar ◽  
Fluorescent Antibody ◽  
Elisa Test ◽  
Hunting Season ◽  
Wild Boars ◽  
North East ◽  
North Eastern ◽  
Diagnostic Sensitivity And Specificity ◽  
Mandatory Vaccination ◽  
First Time

Abstract Background In the last few decades, Romania has been considered one of the European countries most affected by animal rabies, but a combination of oral rabies vaccination (ORV) campaigns in foxes alongside mandatory vaccination of pets has substantially decreased the number of rabies cases in recent years. The objective of this study was to detect rabies antibodies in wild boar serum and thoracic fluid samples collected during the hunting season after ORV campaigns in north-eastern Romania in order to identify if wild boars are substantial competitors to foxes for ORV baits. Results When the 312 wild boar samples were tested by ELISA (BioPro ELISA, Czech Republic), 42.31% (132/312) demonstrated rabies antibodies. In order to compare these wild boar results in terms of the percentage of immunisation, fox samples were also included in the study, and in this case only 28.40% (98/345) demonstrated rabies antibodies by ELISA. To check the diagnostic sensitivity and specificity of this ELISA, those samples with a sufficient volume from both species that had tested either negative or positive with an initial ELISA were then tested with the Fluorescent Antibody Virus Neutralisation (FAVN) assay. The overall concordance between the BioPro ELISA and FAVN test was 74.26% (75/101) in wild boar samples and 65.66% (65/99) in fox samples, 140 out of 200 samples being correlated with the two methods, although no significant statistical difference (p = 0.218) between the two species was registered. We found a good agreement by both tests for the ELISA-positive samples (91.30%), however the situation was different for the ELISA-negative samples, where a low agreement was demonstrated (41.18%). Conclusions This study reports for the first time the presence of rabies antibodies in wild boar samples collected during the hunting season in Romania after ORV campaigns in rabies endemic areas. It is also the first study to demonstrate that ELISA BioPro can be used on wild boar samples with satisfactory results compared to the FAVN test for this species.

scholarly journals Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities

2016 ◽  
Vol 70 (5-6) ◽  
pp. 175-184
Author(s):  
Darko Davitkov ◽  
Srecko Terzic ◽  
Dajana Davitkov ◽  
Milena Radakovic ◽  
Bojan Gajic ◽  
...  
Keyword(s):  
Causative Agent ◽  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Dermacentor Reticulatus ◽  
Species Determination ◽  
Pcr Rflp ◽  
Causative Agents ◽  
Long Time ◽  
Clinically Significant ◽  
Babesia Spp

Babesiosis of domestic animals is a vector transmissible and clinically significant disease, caused by protozoa of genus Babesia and Theileria. Possible causative agents for this disease in dogs in Europe are: Babesia canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis of dogs was for a long time based on the visual inspection of stained blood smear under a microscope, while today there have been increasingly used molecular methods of detection in precise, species diagnostics. The objective of this work was molecular detection of the cause of babesiosis of dogs in the ticks sampled from asymptomatic dogs in the region of some Belgrade municipalities, all for better understanding of epizootiological situation. From three sites in Belgrade, there were collected 49 ticks, sampled from the dogs with no symptoms. There was carried out the determination of the ticks, and after that, DNA was isolated for molecular examination. First, there was performed Polymerase Chain Reaction (PCR), for determining the species of the genus Babesia, and after that there was also carried out the determining of polymorphism in the length of restriction fragments (RFLP) for the purpose of the causative agent species determination. Out of the total number of the examined ticks, 18,34% were positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni was identified, while in 7 cas?es (14,92%) there were no restriction sites for the used enzymes, what suggests that most likely it was B. canis. The ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4 cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This work confirms the presence of Babesia spp. in the ticks sampled from asmptomatic dogs on the teritory of Belgrade as well as the significance of PCR-RFLP method in diagnostics and identification of the causative agent of babesiosis in dogs. For the first time in Serbia, there was determined the presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus)

scholarly journals Konsumsi Mamalia, Burung, dan Reptil Liar Pada Masyarakat Sulawesi Utara dan Aspek Konservasinya

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Saroyo Saroyo
Keyword(s):  
Wild Boar ◽  
Animal Species ◽  
Wild Animal ◽  
Local People ◽  
Breeding Programme ◽  
Animal Population ◽  
Wild Mammals ◽  
Traditional Markets ◽  
North Sulawesi

AbstrakSurvei ini dilaksanakan untuk menginventarisasi jenis-jenis mamalia, burung, dan reptil liar yang dikonsumsi oleh masyarakat Sulawesi Utara dalam kaitannya dengan aspek konservasi dan pemanfaatannya. Metode yang digunakan dalam penelitian ini adalah survei dari tahun 2006 sampai 2010 berdasarkan jenis-jenis yang diperdagangkan di pasar-pasar tradisional, kasus perburuan satwa liar, dan jenis-jenis yang disediakan dalam menu masyarakat pada pesta-pesta adat. Dari survei diperoleh hasil terdapat 39 jenis mamalia, burung, dan reptil liar yang dikonsumsi oleh masyarakat Sulawesi Utara. Konsumsi satwa liar telah menjadi kebiasaan bagi bagi masyarakatnya dan merupakan faktor utama penyebab penurunan populasi satwa liar. Banyak jenis satwa yang dikonsumsi, beberapa termasuk dilindungi, masuk daftar terancam IUCN, dan masuk dalam appendix CITES. Oleh sebab itu pemanfaatan beberapa jenis satwa liar harus mengikuti peraturan perlindungan dan upaya penangkaran tikus ekor putih (Paruromys dominator), babi hutan (Sus celebensis), dan rusa (Cervus timorensis) sangat memungkinkan dan memiliki nilai ekonomi yang tinggi.Kata kunci: burung, mamalia, reptil, Sulawesi UtaraAbstractThis survey was conducted to collect information about wild mammals, birds and reptiles consumed by North Sulawesi people regarding with its conservation aspect and utilization. The used method was survey from 2006 to 2010 based on the animal species sold in some traditional markets, hunting cases and serving food in traditional party menu in Bitung City, Tomohon City and North Minahasa District. The result showed that there were 39 consumed species of wild mammals, birds and reptiles. Consumption of wild animal by local people has become a tradition for the community and it mostly resulted in the decline of wild animal population. Some species are included in IUCN Redlist and CITES Appendices. Therefore, all protection laws should be followed in the utilization of those species. In addition, breeding programme of Sulawesi giant rat (Paruromys dominator), Sulawesi Wild Boar (Sus celebensis) and Timor Deer (Cervus timorensis), is very prospective as it has economically value.Keywords: birds, mammals, reptiles, North Sulawesi

scholarly journals Molecular detection of tick-borne haemopathogens in shelter dogs and Rhipicephalus sanguineus (sensu lato) ticks from Peninsular Malaysia

2020 ◽  
Author(s):  
Quincie Sipin ◽  
Farina Mustaffa Kamal ◽  
Malaika Watanabe ◽  
Puteri Azaziah Megat Abdul Rani ◽  
Nur Mahiza Md ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Rhipicephalus Sanguineus ◽  
Peninsular Malaysia ◽  
Single Infection ◽  
Babesia Gibsoni ◽  
Blood Samples ◽  
Babesia Vogeli ◽  
Microscopic Evaluation ◽  
Rhipicephalus Sanguineus Sensu Lato ◽  
Shelter Dogs

Abstract Background Canine tick-borne haemopathogens (TBH) constitute a significant concern worldwide. The detection of these TBH is mainly achieved by microscopic evaluation, seroprevalence, and molecular detection. The present study was designed to investigate the molecular detection of Anaplasma platys , Babesia gibsoni, Babesia vogeli , and Ehrlichia canis in shelter dogs and Rhipicephalus sanguineus ( sensu lato ) ticks infesting them in Malaysia.Results A total of 220 blood samples and 140 ticks were collected from 10 animal shelters in Peninsular Malaysia. The presence of haemopathogens was detected using conventional PCR, sequenced, and identified at the species level. Of the 220 blood samples, 77 (35%) were positive with at least one of the four haemopathogens, of which E. canis predominated (20%) followed by B. gibsoni and B. vogeli both with detection of 7%, and A. platys (12%). In tick samples, 5 (3.57%) of the collected samples were positive with at least one of the three haemopathogens. Low detection of E. canis and A. platys were present (n = 2; 1.43%) as well as B. vogeli shows only 1 sample positive (0.71%) and no detection of B. gibsoni . For co-infection in dogs, single infection is common (24%), while co-infection with two haemopathogens (10%) was also observed. The occurrence of infection with three TBH was also observed in the sampled dogs (1%).Conclusion E. canis is the most common TBH affecting shelter dogs in Peninsular Malaysia. Co-infection is quite common and the most common co-infection present was E. canis and A. platys. The study highlighted the first extensive molecular detection of TBH in dogs and R. sanguineus ( sensu lato ) ticks in Malaysia

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