scholarly journals Natural hemoplasma infection of cats in Cuiaba, Mato Grosso, Brazil

2018 ◽  
Vol 39 (2) ◽  
pp. 875 ◽  
Author(s):  
Herica Makino ◽  
Daphine Ariadne Jesus de Paula ◽  
Valéria Regia Franco Sousa ◽  
Adriane Jorge Mendonça ◽  
Valéria Dutra ◽  
...  
Keyword(s):  
Blood Count ◽  
Clinical Care ◽  
Mato Grosso ◽  
Blood Samples ◽  
Factors Associated ◽  
Mixed Breed ◽  
The City ◽  
Veterinary Hospital ◽  
Candidatus Mycoplasma Turicensis

The aim of this research was to investigate natural hemoplasma infection in cats treated at the Veterinary Hospital of the Federal University of Mato Grosso, and the factors associated with infection. Blood samples from 151 cats of different sexes, breeds, and ages were analyzed by PCR and blood count. The overall occurrence of hemoplasma was 25.8%. Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum (CMhm)’, and ‘Candidatus Mycoplasma turicensis’ (CMt) were observed in 15.2%, 14.6% and 2.6% of cats, respectively. In 6.6 % of cases, co-infection was observed. Male felines or mixed breed cats were associated with infection by CMhm (P = 0.02 and 0.04, respectively). The data obtained demonstrated an occurrence of 25.8% for hemoplasma infection in felines coming from clinical care in the city of Cuiabá, where males were at higher risk of acquiring the infection by these agents, in addition to a higher risk for CMhm in felines with no specific breed.

scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

scholarly journals Comparing the genetic and environmental architecture of blood count, blood biochemistry and urine biochemistry biological ages with machine learning

2021 ◽  
Author(s):  
Alan Le Goallec ◽  
Samuel Diai ◽  
Theo Vincent ◽  
Chirag J Patel
Keyword(s):  
Machine Learning ◽  
Blood Cells ◽  
Blood Count ◽  
Association Studies ◽  
Blood Biochemistry ◽  
Biological Age ◽  
Genome Wide Association Studies ◽  
Blood Samples ◽  
Factors Associated ◽  
Urine Biochemistry

While a large number of biological age predictors have been built from blood samples, a blood count-based biological age predictor is lacking, and the genetic and environmental factors associated with blood-measured accelerated aging remain elusive. In the following, we leveraged 31 blood count biomarkers measured from 489,079 blood samples, 28 blood biochemistry biomarkers measured from 245,147 blood samples, and four urine biochemistry biomarkers measured from 158,381 samples to build three distinct biological age predictors by training machine learning models to predict age. Blood biochemistry significantly outperformed blood count and urine biochemistry in terms of age prediction (RMSE: 5.92+-0.02 vs. 7.60+-0.02 years and 7.72+-0.04 years). We performed genome wide association studies [GWASs], and found accelerated blood biochemistry, blood count and urine biochemistry aging to be respectively 26.2+-0.3%, 18.1+-0.2% and 10.5+-0.5% GWAS-heritable. We identified 1,081 single nucleotide polymorphisms [SNPs] associated with accelerated blood biochemistry aging, 2,636 SNPs associated with accelerated blood cells aging and 24 SNPs associated with accelerated urine biochemistry aging. Similarly, we identified biomarkers, clinical phenotypes, diseases, environmental and socioeconomic factors associated with accelerated blood biochemistry, blood cells and urine biochemistry aging.

scholarly journals Seroprevalence and risk factors associated with Neospora caninum in crossbred dairy cows in Uberlândia

2018 ◽  
Vol 39 (4) ◽  
pp. 1585 ◽  
Author(s):  
Nayara Resende Nasciutti ◽  
Patrícia Magalhães de Oliveira ◽  
Vinícius De Morais Barbosa ◽  
Selwyn Arlington Headley ◽  
Felipe Gonçalves Garcia ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Cows ◽  
Neospora Caninum ◽  
Reproductive Disorders ◽  
Blood Samples ◽  
Factors Associated ◽  
History Of ◽  
Antibody Titers ◽  
Associated Risk Factors ◽  
The City

Neospora caninum causes reproductive disorders in dairy cattle such as repeat breeding, abortion, and anestrus, which are risk factors associated with the seroprevalence of N. caninum in Brazil and worldwide. This study aimed to verify the risk factors related to the seroprevalence of Neospora caninum in crossbred dairy cows in the city of Uberlândia, Minas Gerais. During, January to July 2013, 740 blood samples were collected from 57 herds with a history of reproductive disorders and determinations of antibody titers against N. caninum were obtained by ELISA assay. The seroprevalence of N. caninum was 18.4% (136/740). Risk factors for N. caninum were cows greater than 2 years of age (P = 0.034), mechanical milking (P = 0.012), and frequent purchase of animals (P = 0.004), in addition to individual history of abortion (P = 0.007) and repeat breeding (P = 0.030). The highest probability of occurrence of associated risk factors was associated with individual history of abortion and repeated breeding and the frequent purchase of animals, with odds of 12.8, 5.9, and 3.8 times, respectively. In conclusion, the risk factors associated with N. caninum: members of the female sex and cows with more than 2 years-of-age, mechanical milking, and the frequent purchase of animals, in addition to individual history of abortion and repeated breeding.

scholarly journals Factors Associated with Malaria Infection among Head Porters in Agbogbloshie Market in the Greater Accra Region of Ghana

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Patience Kwofie ◽  
John Tetteh ◽  
Reindorf Elijah Akakpo ◽  
Bismark Sarfo
Keyword(s):  
Marital Status ◽  
Malaria Infection ◽  
Intervention Programs ◽  
Chi Square ◽  
Blood Samples ◽  
Malaria Intervention ◽  
Factors Associated ◽  
Mobile Population ◽  
The City ◽  
Greater Accra Region

Background. Head porters constitute the mobile population who are at an increasing risk of being infected with malaria. They move around the city to carry out their duties with no accommodation. Therefore, they sleep wherever they find themselves in the evening and do not benefit from most of the malaria intervention programs such as the use of long-lasting insecticide net. The inability to identify them because they are mobile means that they can continue to drive malaria transmission even if malaria in the general population is controlled. Objectives. This study assessed the factors associated with malaria infection among head porters in the Agbogbloshie market in the Greater Accra Region of Ghana. Method. A total of 218 head porters were recruited from the Agbogbloshie market, and blood samples were collected from participants to test for malaria parasite infection using Rapid Diagnostic Test (RDT) and microscopy and were interviewed using a closed-ended questionnaire. The data were analyzed using Stata version 15. Simple descriptive statistics, Pearson chi-square, and Multiple Logistic Regression were performed with significance set at <0.05. Result. The study revealed 12% ( CI   95 % = 8.2 ‐ 16.9 ) and 9.6% ( CI   95 % = 6.3 ‐ 14.4 ) infection of malaria using RDT and microscopy, respectively. Plasmodium falciparum (21/218) was the main parasite detected in all positive blood samples. Age and marital status are significant factors associated with malaria infection among head porters. Age group 40 years and above had 89% ( AOR   0.11   CI   95 % = 0.01 ‐ 0.98 ) reduced odds of getting malaria compared to those below 20 years, while those who are single are 3.52 times more likely to be infected with malaria compared with those who are married ( AOR   95 % CI = 3.52   1.13 ‐ 10.92 ). Conclusion. This study concludes that the increasing age of head porters significantly decreased the probability of malaria infection, while head porters who are single have greater odds of being infected with malaria. Age and marital status are important factors to be considered for malaria intervention programs in head porters.

scholarly journals Prevalence and risk factors to Leptospira sp. infection in dogs attended at veterinary clinics in João Pessoa, Paraíba State, Northeastern Brazil

2018 ◽  
Vol 55 (3) ◽  
pp. e144154 ◽  
Author(s):  
Arthur Willian De Lima Brasil ◽  
Diego Figueiredo Da Costa ◽  
Carla Lauise Rodrigues Menezes Pimenta ◽  
Roberta Nunes Parentoni ◽  
Clebert José Alves ◽  
...  
Keyword(s):  
Risk Factors ◽  
Odds Ratio ◽  
Agglutination Test ◽  
Cutoff Point ◽  
Northeastern Brazil ◽  
Northeast Brazil ◽  
Wild Animals ◽  
Blood Samples ◽  
Factors Associated ◽  
The City

The aim of this study was to determine the prevalence and risk factors associated with Leptospira sp. in dogs attended at veterinary clinics in the city of João Pessoa, State of Paraíba, Northeast Brazil.  A total of 384 blood samples from dogs from 34 veterinary clinics were used from April 2015 to May 2016. The diagnosis of Leptospira sp. was carried out through Microscopic Agglutination Test (MAT), using a collection of 20 pathogenic antigens and adopting a 1: 100 dilution as cutoff point. An epidemiological questionnaire was applied to the owners of the animals to obtain data to be used in the analysis of risk factors. The prevalence of seropositive animals was 11.7% (45/384), with reactions for serogroups Icterohaemorrhagiae (62.3%), Grippotyphosa (22.2%), Canicola (13.3%), Djasiman 2%) and Pomona (2.2%). The following risk factors were identified: age from 49 to 72 months (odds ratio = 2.74), Age > 72 months (odds ratio = 3.22), and monthly cleaning of the environment where the animals are kept (odds ratio = 10,70). It is concluded that dogs attended at veterinary clinics in João Pessoa are exposed to infection by Leptospira sp., with predominance of serogroups kept by wild animals, and it is suggested that the periodic cleaning of the environment where the animals are kept.

scholarly journals Frequency of dogs with anti Leishmania Chagasi antibodies by immunochromatographic assay (DPP) in the city of Sinop, Mato Grosso, Brazil

2020 ◽  
Vol 13 (11) ◽  
pp. 97
Author(s):  
L. M. J. Lopes ◽  
M. V. Bonfim ◽  
F. S. C. Santi ◽  
T. R. Berndt ◽  
J. Bartolomei Neto ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Public Health Problem ◽  
Leishmania Chagasi ◽  
Mato Grosso ◽  
Wide Geographical Distribution ◽  
Health Agencies ◽  
Serious Disease ◽  
City State ◽  
The City ◽  
Veterinary Hospital

Dogs are considered the main reservoir for visceral leishmaniasis infection in humans, a serious disease that is a relevant public health problem due to its wide geographical distribution. To date, there haven’t been notification of Canine Visceral Leishmaniasis (CVL) in official health agencies of Sinop city, state of Mato Grosso. CVL diagnosis is difficult due to the variety of symptoms of the disease. In addition, animals may remain asymptomatic for life. In this study, the frequency of anti Leishmania chagasi antibodies was observed in 201 dogs referred to the clinical routine of the Veterinary Hospital of the Federal University of Mato Grosso, Campus of Sinop, during a 67 days period, through immunochromatographic method (DPP). Obtained results showed that seven animals were positive while one hundred ninety four were negative to the test. This result is relevant as far as it may increase the risk of CVL dispersion to non-endemic areas, highlighting the need of implementation effective measures to quickly detect Leishmania chagasi and prevent epidemiological situations.

scholarly journals Hemotropic mycoplasmas in naturally infected cats in Northeastern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 446-454
Author(s):  
Alexandre Dias Munhoz ◽  
Izabela Garcia Pinto Coelho Simões ◽  
Ana Paula Fernandes Calazans ◽  
Ludimila Santos Macedo ◽  
Rebeca Dálety Santos Cruz ◽  
...  
Keyword(s):  
Northeastern Brazil ◽  
Laboratory Findings ◽  
Blood Samples ◽  
Factors Associated ◽  
Serum Biochemical ◽  
Complete Blood Counts ◽  
Laboratory Changes ◽  
Candidatus Mycoplasma Turicensis

Abstract This study aimed to determine the prevalence, factors associated, laboratory findings (with and without coinfection by retroviruses) among naturally infected cats by hemoplasmas in northeastern Brazil. For convenience, 200 domesticated and healthy cats were selected. Blood samples were taken to perform complete blood counts, serum biochemical, immunochromatography tests and nPCR for FIV and FeLV, and PCR for hemoplasma recognition. An interview was conducted to determine the factors associated with hemoplasmas. A total of 71/200 (35.5%) cats were positive for at least one hemoplasma species. Isolated infections were observed in 12,5% for 'Candidatus Mycoplasma haemominutum', 12% for Mycoplasma haemofelis and 3% for 'Candidatus Mycoplasma turicensis'. Regarding copositivity, 2% of the animals were positive for M. haemofelis and 'Candidatus Mycoplasma haemominutum', 1.5% for M. haemofelis and 'Candidatus Mycoplasma turicensis', and 4.5% for ' Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis'. No clinical and laboratory changes were observed in the animals that were concomitantly positive for retroviruses and hemoplasmas. Periurban region cats were more likely to be infected by M. haemofelis, while contact with other cats and infection by ' Candidatus Mycoplasma turicensis' were associated with 'Candidatus Mycoplasma haemominutum'. This study indicates that infection by hemoplasmas is a common find in cats from northeastern Brazil.

scholarly journals Factors associated with the functional independence of elderly women in the city of Cuiabá

2016 ◽  
Vol 19 (5) ◽  
pp. 827-837
Author(s):  
Idilaine de Fátima Lima ◽  
Rosemeiry Capriata de Souza Azevedo ◽  
Annelita Almeida Oliveira Reiners ◽  
Ageo Mario Cândido da Silva ◽  
Luciane Cegati de Souza ◽  
...  
Keyword(s):  
Physical Activity ◽  
Healthy Aging ◽  
Elderly Women ◽  
Prevalence Ratio ◽  
Functional Independence ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Mato Grosso ◽  
Factors Associated ◽  
The City

Abstract Objective: To analyze the prevalence of and factors associated with functional independence among community based elderly women. Methods: A cross-sectional study was conducted in the urban area of the city of Cuiabá, in the state of Mato Grosso, involving 247 women aged 60 and over. Data was collected through interviews, using instruments such as the Mini Mental State Examination, a questionnaire about demographic and health data, the Katz Index and the Lawton and Brody Scale. Prevalence ratio and the chi-squared test (p=0.05) were used as measures of association, whereas for multivariate analysis, the Poisson regression model was used. Calculations were performed with the Statistical Package for Social Sciences 22.0 program. Results: The prevalence of functional independence was 63.2%. The variables associated with independence were a younger age, an income greater than the minimum wage; the use of up to two drugs, did not need hospitalization in the last 6 months, had not experienced immobilization that prevented locomotion after age 60, visiting friends and relatives, social participation and physical activity. Conclusion: All the variables were strongly associated with healthy aging. Even in the presence of pathologies considered common to the aging process, the practice of physical activity and social interaction are important markers of functional independence.

scholarly journals Erythrocyte phenotyping for feline AB system in domestic cats from the Ilhéus-Itabuna microregion, Bahia, Brazil

2021 ◽  
Vol 41 ◽  
Author(s):  
Jamille B. Carvalho ◽  
Camilla F. Oliveira ◽  
Paula Elisa B. Guedes ◽  
Katharine C. Santos ◽  
Alexandre D. Munhoz ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Home Visits ◽  
Domestic Cats ◽  
Santa Cruz ◽  
Blood Samples ◽  
Mixed Breed ◽  
The Cross ◽  
Veterinary Hospital ◽  
Made In

ABSTRACT: This study aimed to determine the erythrocyte phenotypes of the feline AB system and to check the presence of antigens other than those present in the feline AB system in domestic cats from Ilhéus-Itabuna microregion, Bahia, Brazil. Three-hundred feline blood samples were collected at the Veterinary Hospital of the “Universidade Estadual de Santa Cruz” (UESC) and in home visits to perform blood phenotyping using the tube-method testing. The reverse phenotyping was made between cats that tested phenotype B with blood samples of cats that tested phenotype A to confirm the blood phenotype B. The cross-tested among cats with phenotype A was made in order to verify the presence of different antigens of AB system in this blood phenotype. The results underwent macroscopic and microscopic analyses. Among the 300 animals tested, regarding breed, 290 were mixed-breed cats and among the remaining ten, five were Persians, four Siamese, and one Angora. 297 (99%) presented with phenotype A (including all the breeding cats) and three (1%) with phenotype B, and all this cats were mixed-breed cats. None (0%) of the cats showed the phenotype AB. All phenotype B bloods reacted to reverse phenotyping with phenotype A, confirming the phenotype B of these cats. All phenotype A bloods were compatible among each other, so no further erythrocyte antigens were detected through this test. The mother of one of the phenotype B cats was identified and had phenotype A, demonstrating phenotype A parents with phenotype B offspring. This finding indicates heterozygosis in the studied population. This data enable to conclude that the studied population presented different erythrocyte phenotypes, subsequently highlighting the importance of conducting phenotype analyses in these animals before performing blood transfusion to avoid serious hemolytic complications associated with incompatibility.

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