scholarly journals Parasites in the mangrove oyster Crassostrea rhizophorae cultivated in the estuary of the Graciosa River in Taperoá, Bahia

2015 ◽  
Vol 24 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Aliane Watanabe Cova ◽  
Moacyr Serafim Júnior ◽  
Guisla Boehs ◽  
Jackson Moreira de Souza
Keyword(s):  
Water Quality ◽  
Optical Microscope ◽  
Water Salinity ◽  
Monthly Basis ◽  
Histological Techniques ◽  
Low Intensity ◽  
Hematoxylin And Eosin ◽  
Neanthes Succinea ◽  
Crassostrea Rhizophorae

In this work, parasites associated with the mangrove oyster Crassostrea rhizophorae were studied at a cultivation unit in the estuary of the Graciosa River in Taperoá, Bahia. On a monthly basis, between April 2011 and March 2012, 20 oysters were collected, measured and fixed in 10% formaldehyde. In order to identify the parasites, routine histological techniques were used. The specimens were embedded in paraffin and sections, 7 µm thick, were cut. These were then stained with Harris hematoxylin and eosin and were examined using an optical microscope. The parameters analyzed to investigate water quality showed that the temperature ranged from 23.9 °C to 29.3 °C, water salinity from 0.4 to 24.2 Practical Salinity Units PSU and recorded rainfall from 80 mm to 406.4 mm/month. During the parasitological analyses, infestation of the polychaete Neanthes succinea was observed in 41% of the oysters. Through histological techniques, the following parasites were identified: colonies of Rickettsia-like organisms (RLOs); the protozoa Nematopsis sp. and Ancistrocoma sp.; the turbellarian Urastoma sp.; the metacestode Tylocephalum sp. and an unidentified tapeworm. The data collected showed that there was low-intensity infection with and prevalence of parasites, except for Nematopsis sp., thus attesting that these cultivated oysters were in a healthy condition.

scholarly journals PHYSICOCHEMICAL AND BIOLOGICAL WATER QUALITY ASSESSMENT OF LAKE HAWASSA FOR MULTIPLE DESIGNATED WATER USES

2016 ◽  
Vol 9 (2) ◽  
pp. 146-157
Author(s):  
Adimasu Woldesenbet Worako
Keyword(s):  
Water Quality ◽  
Water Quality Assessment ◽  
Quality Parameters ◽  
Ethiopian Rift ◽  
Trophic State Index ◽  
Monthly Basis ◽  
Biological Water ◽  
State Index ◽  
Rift Valley Lakes ◽  
Biological Water Quality

Lake Hawassa is one of the Major Ethiopian Rift Valley Lakes which is situated in southern regional state, which has a closed basin system and receives water from only Tikurwuha River and runoff from the catchment. Quality of the lake water is vital for the surrounding community for proper and safe use of the lake. The present study was designed to examine the physicochemical and biological water quality suitability for multiple purposes and to determine trophic state index of the lake for a period of three months from December to February, 2011/12. Water samples were collected from the lake on monthly basis and analyzed for all water quality parameters by using standard methods. Data analysis was performed by descriptive, multivariate analysis (MANOVA%

scholarly journals THE USE OF PATH ANALYSIS IN THE DETERMINATION OF ENVIRONMENTAL FACTOR EFFECTS ON THE TOTAL PRODUCTION OF AQUACULTURE PONDS IN PASURUAN, EAST JAVA PROVINCE

2016 ◽  
Vol 10 (2) ◽  
pp. 173
Author(s):  
Andi Indra Jaya Asaad ◽  
Erna Ratnawati ◽  
Akhmad Mustafa
Keyword(s):  
Water Quality ◽  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Path Analysis ◽  
Soil Quality ◽  
Water Salinity ◽  
Total Production ◽  
Direct Effects ◽  
Technical Application ◽  
Soil And Water

Environmental factors in the form of soil and water quality are the important factors of aquaculture pond productivity, including total production (tiger shrimps, Penaeus monodon, and milkfish, Chanos chanos) in Pasuruan, East Java Province. The objective of this study was to analyze the direct or indirect effects of soil and water quality on the total production of ponds in Pasuruan using a path analysis application. Data were collected in the pond areas around Pasuruan Regency including Nguling, Lekok, Rejoso, Keraton, and Bangil Sub-Districts as well as Pasuruan City. Soil quality was determined as a free variable and exogen; water quality as mediate variable, suspended, and endogen; as well as milkfish production as suspended variable and endogen. Environmental characteristics were illustrated using descriptive statistics, while environment factor effects on total production were analyzed using path analysis. The results of path analysis show that from the 12 analyzed soil quality variables, only two variables were affected in the total production of pond (tiger shrimps and milkfish) namely: contents of soil organic carbon and soil phosphate. While based on 11 water quality variables, two variables (water salinity and water iron) were affected the total production of ponds in Pasuruan Regency. The direct effects of soil organic carbon and phosphate on the total production were 0.314 and -0.600, respectively. Water salinity and water iron gave direct effects on total production amounting to -0.678 and 0.358 respectively. It is also found that two soil variables which were affected in the total production, did not indicate the effect towards water quality in ponds. Further implication of this research is put more attention for these variables into pond’s management in order to gain more production. Technical application could be appropriate for pond preparation and frequently water changing during grow out.

scholarly journals Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Cells

2012 ◽  
Vol 92 (9) ◽  
pp. 1208-1219 ◽  
Author(s):  
Cheng Zhong ◽  
Xin Zhang ◽  
Zhengjian Xu ◽  
Rongxin He
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Electromagnetic Fields ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Low Intensity ◽  
Hematoxylin And Eosin

Background Electromagnetic fields (EMFs) used in stem-cell tissue engineering can help elucidate their biological principles. Objective The aim of this study was to investigate the effects of low-intensity EMFs on cell proliferation, differentiation, and cycle in mouse bone marrow stromal cells (BMSCs) and the in vivo effects of EMFs on BMSC. Methods Harvested BMSCs were cultured for 3 generations and divided into 4 groups. The methylthiotetrazole (MTT) assay was used to evaluate cell proliferation, and alkaline phosphatase activity was measured via a colorimetric assay on the 3rd, 7th, and 10th days. Changes in cell cycle also were analyzed on the 7th day, and bone nodule formation was analyzed on the 12th day. Additionally, the expression of the collagen I gene was examined by reverse transcription-polymerase chain reaction (RT-PCR) on the 10th day. The BMSCs of the irradiated group and the control group were transplanted into cortical bone of different mice femurs separately, with poly(lactic-co-glycolic acid) (PLGA) serving as a scaffold. After 4 and 8 weeks, bone the bone specimens of mice were sliced and stained by hematoxylin and eosin separately. Results The results showed that EMFs (0.5 mT, 50 Hz) accelerated cellular proliferation, enhanced cellular differentiation, and increased the percentage of cells in the G2/M+S (postsynthetic gap 2 period/mitotic phase + S phase) of the stimulation. The EMF-exposed groups had significantly higher collagen I messenger RNA levels than the control group. The EMF + osteogenic medium–treated group readily formed bone nodules. Hematoxylin and eosin staining showed a clear flaking of bone tissue in the irradiated group. Conclusion Irradiation of BMSCs with low-intensity EMFs (0.5 mT, 50 Hz) increased cell proliferation and induced cell differentiation. The results of this study did not establish a stricter animal model for studying osteogenesis, and only short-term results were investigated. Further study of the mechanism of EMF is needed.

FEATURES OF THE PATHOMORPHOLOGICAL DIAGNOSIS OF MICROCRYSTALLINE ARTHROPATHIES IN THE PRACTICE OF SURGICAL MATERIAL EXAMINATION

2020 ◽  
Vol 58 (3) ◽  
pp. 286-289
Author(s):  
N. S. Migalkin ◽  
T. A. Stupina ◽  
A. V. Kaminsky ◽  
D. S. Mokhovikov ◽  
D. A. Shabalin ◽  
...  
Keyword(s):  
Staining Technique ◽  
Calcium Pyrophosphate ◽  
Alcohol Solution ◽  
Formalin Fixation ◽  
Histological Techniques ◽  
Tissue Sections ◽  
Stereo Microscope ◽  
Hematoxylin And Eosin ◽  
Tissue Specimens ◽  
Msu Crystals

The development of microcrystalline arthritides is most frequently associated with the formation of monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals. Their identification is of crucial importance in recognizing these diseases. Objective: to determine the possibilities of histological techniques in identifying MSU and CPP crystals and to evaluate the effectiveness of the techniques. Subjects and methods. Twenty-four tissue blocks (fragments of the affected areas of the elbow joint, the interphalangeal joint of the index finger, and hip joint) from 7 patients were examined. Paraffin sections were stained with a 0.5% alcohol solution of eosin, as well as with hematoxylin and eosin. Tissue specimens were examined and digitized using an AxioScope.A1 stereo microscope with Zenblue software (Carl Zeiss MicroImaging GmbH, Germany). Results and discussion. When staining the tissue sections with hematoxylin and eosin, microcrystals were not visualized; the major portions of MSU crystals was dissolved during fixation and staining, whereas CPP crystals were masked with hematoxylin as focal basophilic aggregates. The staining technique with an alcohol solution of eosin and short formalin fixation (within 12 hours) made it possible to avoid dissolution of MSU crystals and to visualize both MSU and CPP crystals, and to determine their shape and color. Conclusion. Light microscopy of the tissue sections stained with an alcohol solution of eosin along with short formalin fixation is a reliable method to differentiate MSU and CPP crystals. In patients undergoing endoprosthetic replacement, the significance of this technique for the pathomorphological study of surgical material consists in assessing inflammatory activity and in eliminating a disease, such as microcrystalline arthropathy.

scholarly journals ASSESSING WATER QUALITY OF BOYALI DAM LAKE (SINOP, TURKEY) BY USING ECOLOGICAL AND STATISTICAL INDICATORS

2021 ◽  
Vol 20 (1) ◽  
pp. 77-85
Author(s):  
Ekrem Mutlu ◽  
◽  
Naime Arslan ◽  
Cem Tokatli ◽  
◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Quality Index ◽  
Quality Index ◽  
Temporal Variations ◽  
Quality Parameters ◽  
World Health ◽  
Water Quality Control ◽  
Monthly Basis ◽  
Quality Material

Aim of the study: In the present study, the spatial – temporal variations of water quality in Boyalı Pond were analyzed. Water Quality Index (WQI) based on the World Health Organization's standards specified for drinking water, and Water Quality Control Regulations in Turkey (WQCR), as well as certain multi-statistical methods, were used in analyzing the water quality. Material and methods: Water samples were collected from 5 stations selected in the lake on monthly basis in 2019 and 30 water quality parameters were measured in total. Water Quality Index (WQI), Factor Analysis (FA), and Cluster Analysis (CA) were used in order to determine the differences between the spatial and temporal quality levels and to classify the investigated locations. Results and conclusions: According to data observed, Boyalı Dam Lake was found to have Class I and Class II water quality in general the WQI results obtained suggested that, although the water quality was found to significantly decrease in summer months, the reservoir was found to have an "A Grade – Excellent" water quality (<50) in all the months and stations analyzed here. WQI values recorded in the dam lake ranged between 16.4 and 27.8 and the detected limnologic parameters did not exceed the standards specified for drinking water in any of the investigated months and stations (<50 for WQI). As a result of FA, 3 factors explained 88.9% of total variances and as a result of CA, 2 statistical clusters were formed.

scholarly journals Potential of Floating Photovoltaic Technology and Their Effects on Energy Output, Water Quality and Supply in Jordan

Energies ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 8417
Author(s):  
Mohamad Al-Widyan ◽  
Mohammad Khasawneh ◽  
Muna Abu-Dalo
Keyword(s):  
Water Quality ◽  
Weather Conditions ◽  
Quality Parameters ◽  
Energy Output ◽  
Photovoltaic Systems ◽  
Average Increase ◽  
Data Logging ◽  
Floating Structure ◽  
Monthly Basis ◽  
Semi Arid Region

In this work, floating photovoltaic systems were experimentally studied under Jordan’s weather conditions to determine their effects on energy output, water quality and supply. A limited number of studies have addressed the effect of floating photovoltaic systems on water quality and evaporation reduction especially in a semi-arid region like Jordan. Energy measurements were taken from August 2020 to January 2021 using an Arduino board with data logging sensors. Water quality parameters were tested for collected samples on a monthly basis from August 2020 to February 2021 using a spectrophotometer. Results revealed that the floating panel temperature was lower than the ground-mounted counterpart. An average increase of 1.68% in voltage and 4.40% in current were observed for the floating panel compared to the ground-mounted panel which translates to an average increase of 5.33% in power generation over the ground-mounted panel. Furthermore, efficiency and fill factor increased by 4.89% and 5.51%, respectively. Evaporation results showed that covering water bodies with panels can save a considerable amount of water. Over a period of 30 days, the 30% coverage pan saved 31.2% (36 mm) of water while the 50% coverage pan saved 54.5% (63 mm) of water in the same period compared to the uncovered pan. Moreover, this study involved examining the effect of shading caused by the floating structure on water quality. Results showed a reduction in pH, improvement in transparency, and an increase in total organic carbon indicating water quality enhancement and algal biomass reduction. However, due to the respiration of algae, the dissolved oxygen declined significantly, accompanied by the release of phosphate due to algae decomposition. Overall, findings of this research provided better understanding of floating photovoltaic systems and their applicability in Jordan to provide a safe and reliable supply of water and energy. Additionally, such systems can help to diversify the energy mix and help Jordan to alleviate some of the problems associated with limited energy and water resources.

scholarly journals Parasitism by a Digenea in Lucina pectinata (Mollusca: Lucinidae)

2017 ◽  
Vol 78 (1) ◽  
pp. 94-97
Author(s):  
M. M. Ribeiro ◽  
J. B. Oliveira ◽  
G. Boehs
Keyword(s):  
River Estuary ◽  
Microscopic Analysis ◽  
Optical Microscope ◽  
Mangrove Sediment ◽  
Brazilian Coast ◽  
Economic Resource ◽  
Lucina Pectinata ◽  
Hematoxylin And Eosin ◽  
Chemical Conditions ◽  
Axis Length

Abstract Lucina pectinata is an important economic resource in the Brazilian coast. This study reports parasitism caused by a Digenea in this species. The specimens (n = 470) were collected in December 2012 in a mangrove swamp of the Cachoeira River estuary, Bahia, Brazil. They were measured along the anterior-posterior axis (length), and after macroscopic analysis for parasites and diseases cuts of 5 mm were fixated in Carnoy’s solution and processed by routine histology technique wherein sessions of 7 μm were stained with Harris hematoxylin and eosin (H&E). The tissues were examined using an optical microscope. The mean length of L. pectinata was 4.0 ± 0.53 cm. Microscopic analysis showed sporocysts containing both germ balls as cercariae of an unidentified Digenea (Platyhelminthes), these in various stages of development. The prevalence was 1.48% (7/470). In a parasitized specimen was macroscopic evidence of tissue densification of gills. The sporocysts were observed in mantle, gills, digestive gland and gonads, with evident alteration/destruction of tissues, including parasitic castration. There were no other parasites found, which is probably related to inaccessibility and chemical conditions in which lives L. pectinata, i.e., between 10 and 20 cm in mangrove sediment.

scholarly journals Parasites of the mangrove mussel Mytella guyanensis (Bivalvia: Mytilidae) in Camamu Bay, Bahia, Brazil

2012 ◽  
Vol 72 (3) ◽  
pp. 421-427 ◽  
Author(s):  
LO. Ceuta ◽  
G Boehs
Keyword(s):  
Temporal Variation ◽  
Water Temperature ◽  
Digestive Gland ◽  
High Intensity ◽  
Microscopic Analysis ◽  
Intensity Of Infection ◽  
Histological Techniques ◽  
Parasitic Castration ◽  
High Prevalence ◽  
Hematoxylin And Eosin

This contribution reports the parasites found in the mangrove mussel Mytella guyanensis in Camamu Bay, Bahia, Brazil. Samples were collected monthly from September 2006 through October 2007. A total of 460 individuals were collected, fixed in Davidson's solution, and processed by standard histological techniques, and the sections were stained with Harris hematoxylin and eosin (H&E). The water temperature ranged from 23.5 to 31.6 ºC, and the salinity from 25 to 37‰. Microscopic analysis showed Rickettsia-like organisms (RLOs), Nematopsis sp. (Apicomplexa), and Platyhelminthes, including a turbellarian, sporocysts of Bucephalus sp., metacercariae, and metacestodes of Tylocephalum sp. Parasites were observed mainly in the gills, mantle, and digestive gland. The prevalence of Nematopsis sp. was 100%, and in heavily infected mussels the tissues of the labial palps were damaged. RLOs occurred in high prevalence and intensity of infection in some periods. The digenean sporocysts showed moderate prevalence but high intensity of infection, and caused parasitic castration. In general, there was no significant spatial or temporal variation (p > 0.05) of the parasites, which is probably attributable to the small variations of temperature and salinity in the region.

scholarly journals Mangrove oyster (Crassostrea rhizophorae) (Guilding, 1928) farming areas as artificial reefs for fish: A case study in the State of Ceará, Brazil

2006 ◽  
Vol 54 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Luiz Eduardo Lima de Freitas ◽  
Caroline Vieira Feitosa ◽  
Maria Elisabeth de Araújo
Keyword(s):  
Water Quality ◽  
Artificial Reefs ◽  
Visual Census ◽  
Ecological Aspects ◽  
Physical And Chemical Variables ◽  
Crassostrea Rhizophorae ◽  
Tidal Variations ◽  
Physical And Chemical ◽  
Permanent Residents

A type of platform, known as a table, is now being used for mangrove oyster farming. In Fortim, Ceará, Brazil, this activity was begun in June 2000 and covers an area of 50 m² overlying a sand-clay substrate. The present study has the following main objectives: to identify and catalogue the ichthyofauna colonizing the Crassostrea rhizophorae farming platforms; to evaluate ecological aspects, such as the possible correlation between the physical and chemical variables for water quality and the occurrence of the ichthyofauna; and to observe the differences in the fish species found during tidal variations. Specimens were identified and quantified using the linear-transect, visual census methodology. The ichthyofauna observed comprised 3,030 individuals belonging to 28 species and 20 families. Of the 28 species found in the area studied, 14 were marine transients, 12 marine dependent, and only 2 permanent residents. A significant association was observed between the abundance of 11 species and the physical and chemical variables studied. Based on these results, it may be concluded that the platforms act as artificial reefs for the ichthyofauna, being colonized by at least 28 species, and providing protection from predators as well as a source of food and a reproductive substrate.

scholarly journals The Correlation Between Plankton Abundance And Water Quality in Donan River

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (3) ◽  
pp. 14
Author(s):  
Nuraina Andriyani ◽  
Arif Mahdiana ◽  
Endang Hilmi ◽  
Samuel Kristian
Keyword(s):  
Water Quality ◽  
River Water ◽  
Aquatic Ecosystem ◽  
Random Sampling ◽  
Water Salinity ◽  
Lake Ecosystem ◽  
Ocean Ecosystem

Planktons  have ability to live in aquatic ecosystem, including rivers ecosystem, estuary ecosystem, lake ecosystem and ocean ecosystem. The plankton abundance is estimated by correlation with water quality both of salinity, pH, temperature and others. This research aimed to analysis plankton abundance and to analysis correlation between plankton abundance and water quality. This research was conducted in Donan River using random sampling in 10 stations. The plankton are collected  using plankton net no 25 and Lackey Drop Mikrotranset Counting (plankton analysis) and APHA (2005) to analysis water quality. The results showed that the plankton abundance ranged between 524 – 6,406 ind L-1 and best correlation with water salinity had index 0.975. Keywords: plankton abundance, water quality, Donan river, water salinity, correlation

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