scholarly journals New approaches to studying morphological details of intramolluscan stages of Angiostrongylus vasorum

2020 ◽  
Vol 29 (2) ◽  
Author(s):  
Cíntia Aparecida de Jesus Pereira ◽  
Aytube Lucas Coaglio ◽  
Luciano Santos Capettini ◽  
Raphael Becattini ◽  
Ana Paula Pereira Neves Ferreira ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Distal Portion ◽  
Angiostrongylus Vasorum ◽  
Anterior Region ◽  
Achatina Fulica ◽  
Initial Portion ◽  
Baermann Technique ◽  
Wild Canid ◽  
Picrosirius Red ◽  
Oil Red O

Abstract Angiostrongylus vasorum is a pulmonary artery parasite of domestic and wild canid. On molluscs, intermediate host, first stage larvae (L1) are found after the first day of infection, in the 8th L2 and in the 30 th L3. It was evaluated L1, L2 and L3 recovered by Baermann technique from Achatina fulica infected with 1000 L1. Fifty larvae/stage were incubated with antibodies anti-β-tubulin, anti-α-tubulin, anti- α-actin, anti-β-actin and anti-collagen, and then with Alexa 633. Fifty larvae/stage were observed with picrosirius red and Oil Red O. It was also observed in the anterior region of L1 the beginning of the chitinous stems development, in the initial portion of the intestine and genital primordium. In L2 anterior region, the papillae, chitinous canes juxtaposed to the mouth and intestines bigger than L1. The L3 musculature is well defined, next to the chitinous stems, there are two round distally arranged from each other. It was observed the whole extension of the intestine genital primordium and intense cellularity in the L3 distal portion. With the picrosirius red the L1, L2 and L3 musculature could be observed, as the nerve ganglia on L3. Oil Red O revealed that L1, L2 and L3 store energy on lipid droplets.

CASE REPORT: LIPOSARCOMA WITH MICROVASCULAR PROLIFERATION IN A COCKATIEL (Nymphicus hollandicus)

2019 ◽  
Vol 45 (03) ◽  
pp. 85-89
Author(s):  
Jung-Chin Chang ◽  
Fang-Yi Tsai ◽  
Yu-Shing Lee ◽  
Ruo-Chan Wang ◽  
Ju-Pai Kao ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Lipid Droplets ◽  
Excisional Biopsy ◽  
Positive Staining ◽  
Uropygial Gland ◽  
Microvascular Proliferation ◽  
Nymphicus Hollandicus ◽  
Well Differentiated ◽  
Oil Red O ◽  
Growing Mass

A five-year-old female cockatiel weighing 117 g was presented with a fast-growing mass beside the uropygial gland. Excisional biopsy was performed and the mass measured [Formula: see text][Formula: see text]cm in size and weighed 30.6 g. On the surface of cut sections, the mass was yellow-brown with white or yellow colloidal substances and red exudate. Histopathology showed that the tumor mass was covered by the skin and located in the deep dermis and hypodermis. The tumor consisted of abundant vascular adipose tissue and lipoblasts with intracytoplasmic lipid droplets, which varied in size. Also, small, well-differentiated blood vessels, with varied degrees of congestion and dilation, were observed within the tumor. Histochemically, staining with Oil red O produced a positive reaction in which the lipid droplets presented a reddish color. Immunohistochemistry produced positive staining for Desmin and successfully marked the muscular layers of blood vessels. On the basis of these results, a rare case of liposarcoma with microvascular proliferation adjacent to the uropygial gland was diagnosed in a cockatiel.

scholarly journals ANTIHYPERGLYCEMIC ACTIVITY OF TINOCRISPOSIDE BY STIMULATING 3T3-L1 ADIPOCYTE CELL DIFFERENTIATION

2018 ◽  
Vol 11 (11) ◽  
pp. 494
Author(s):  
Adek Zamrud Adnan ◽  
Muhammad Taher ◽  
Annisa Fauzana ◽  
Tika Afriani ◽  
Dewi Imelda Roesma ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Lipid Droplets ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Antihyperglycemic Activity ◽  
Significant Difference ◽  
Adipocyte Cell ◽  
Oil Red O Staining ◽  
Oil Red O

Objective: The aim of this study is to investigate the antihyperglycemic activity of tinocrisposide by stimulating 3T3-L1 adipocyte cell differentiation. Tinocrisposide is a furanoditerpene glycoside that was isolated from Tinospora crispa (Menispermaceae).Methods: Adipocyte cell differentiation activity of tinocrisposide in interval concentrations of 50, 25, 12.5, and 6.25 μg/ml has been investigated on 3T3-L1 cell line using insulin of 1 μg/ml as a positive and Dulbecco’s modified Eagle media (DMEM) as a negative control group. The effect of tinocrisposide was quantified with oil red O staining method by measuring an absorbance of lipid solution in isopropanol at a wavelength (λ) of 520 nm.Results: Tinocrisposide in the concentrations of 50, 25, 12.5, and 6.25 μg/ml insulin of 1 μg/ml and DMEM groups showed absorbance value of 0.7669, 0.7253, 0.6563, 0.6481, 0.954, and 0.2653, respectively. It was found that there was a significant difference statistically in lipid droplets accumulation among all groups (p<0.05) and tinocrisposide at a concentration of 50 μg/ml showed the highest lipid droplets accumulation in 3T3-L1 adipocyte cells.Conclusion: From the study, it could be concluded that tinocrisposide was able to stimulate the differentiation of adipocyte cell and had antihyperglycemic activity.

scholarly journals Korean Traditional Fermented Soybean Paste (Doenjang) Regulate Renin-Angiotensin System (RAS) in 3T3-L1 Adipocytes

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 791-791
Author(s):  
Hayoung Woo ◽  
Jung Eun Park ◽  
Youn-Soo Cha
Keyword(s):  
Blood Pressure ◽  
Lipid Accumulation ◽  
Lipid Droplets ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Fermented Soybean ◽  
Soybean Paste ◽  
Oil Red O Staining ◽  
Oil Red O

Abstract Objectives Doenjang, the Korean traditional fermented soybean paste, contains much salt. There is a concern that cardiovascular disease may occur due to such high salinity. Nevertheless, previous studies have demonstrated functional properties of doenjang anti-obesity and anti-cancer effects. Furthermore, in our recent studies, we showed that the anti-hypertensive effect of doenjang through renin-angiotensin system (RAS) regulation. Doenjang regulated the RAS to improve lipid metabolism in adipose tissue, which had a positive effect on blood pressure control. Therefore, we expected to find the exact mechanism of action or target point of doenjang in adipocyte using 3T3-L1 cells. Methods In this study, 3T3-L1 cells were treated with doenjang and RAS blockers, Losartan (10−4 M), and Captopril (10−4 M), were treated as positive control which suppresses AT1R and ACE, respectively. Non-cytotoxic concentrations of samples were selected as per MTT assay and added with induction media, harvested after 4 days for RNA extraction. Lipid droplets were detected by Oil Red O staining. Results Doenjang downregulated mRNA levels of peroxisome proliferator-activated receptor-γ (Pparg), RAS related genes such as angiotensinogen (Agt), Renin (Ren), and aldosterone-releasing factors (P &lt; 0.05). Especially, angiotensin convert enzyme (Ace) and angiotensin II receptor 2 (Agtr2) levels were decreased by doenjang treatment. Doenjang reduced the lipid accumulation, which was confirmed from the Oil Red O staining of lipid droplets. As a result, it is revealed that doenjang not only inhibits lipid accumulation in adipocytes but also may inhibit ACE in 3T3-L1 adipocytes through a mechanism similar to the effect of Captopril. Conclusions These data are consistent with our animal study. It have been shown to regulate blood pressure through lipid improvement and ACE inhibition despite high salt content in doenjang. Funding Sources This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 2018R1A2B6006477).

scholarly journals Upregulated microRNA-106a Promotes Porcine Preadipocyte Proliferation and Differentiation by Targeting Different Genes

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 805 ◽  
Author(s):  
Kuilong Huang ◽  
Xin’e Shi ◽  
Jie Wang ◽  
Ying Yao ◽  
Ying Peng ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lipid Droplets ◽  
Luciferase Reporter ◽  
Cell Cycles ◽  
Lipogenic Genes ◽  
Proliferation And Differentiation ◽  
Luciferase Reporter Vector ◽  
Membrane Bound ◽  
Porcine Adipocytes ◽  
Oil Red O

Adipose tissue is one of the main organs for the energy storage and supply of organisms. Adipose deposition and metabolism are controlled by a cascade of transcription factors and epigenetic regulatory mechanisms. Previous studies have also shown that miR-106a plays a considerable role in the development of organisms. The regulatory mechanism of miR-106a on porcine preadipocytes is still not clear. In this study, preadipocytes were isolated from the neck subcutaneous deposits of 3–5-day old Chinese native Guanzhong black pigs using 5-ethynyl-20-deoxyuridine (EdU) staining and a CCK-8 assay to detect the number of proliferous cells and real-time qPCR (RT-qPCR) and western blot analysis to detect gene expression, as well as Oil Red O and BODIPY staining dye lipid droplets and flow cytometry (FCM) to detect cell cycles. We also used the double luciferase method to detect the relative luciferase activities. Upregulated miR-106a increased the number of proliferous cells and enhanced the expression of cell proliferation-related genes in porcine adipocytes. The double luciferase reporter vector confirmed that p21 was a target gene of miR-106a in the cell proliferation phase. miR-106a upregulation increased the number of lipid droplets and the expression of lipogenic genes and directly targeted BMP and activin membrane-bound inhibitor (BAMBI) in the process of differentiation. Our results indicated that miR-106a promotes porcine preadipocyte proliferation and differentiation by targeting p21 and BAMBI.

Secreted-Osteopontin Contributes to Brown Adipogenesis In Vitro via a CD44-Dependent Pathway

2019 ◽  
Vol 51 (11) ◽  
pp. 741-748
Author(s):  
Mengxi Wang ◽  
Yaoyao Guo ◽  
Yumeng Zhou ◽  
Wanwan Yuan ◽  
Huixia Li ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Biological Activities ◽  
Tumor Formation ◽  
Brown Adipocyte ◽  
Brown Adipocytes ◽  
Protein Levels ◽  
Infected Cells ◽  
Oil Red O Staining ◽  
Oil Red O

AbstractOsteopontin (OPN), a secreted glycoprotein, is involved in various pathophysiological processes including immune response, inflammation, tumor formation, and metabolism. OPN exists in 2 forms, secreted-OPN (sOPN) and intracellular-OPN (iOPN). While they might have different biological activities, it remains largely unknown whether sOPN and iOPN induce the differentiation of brown adipocytes. To test this possibility, 3T3-L1 cells were induced by DMI induction with or without recombinant human OPN (rhOPN, 10, 50, 100, 200 μM), respectively. Meanwhile, another batch of 3T3-L1 cells were infected with Ad-GFP-ap2-OPN and followed by DMI differentiation. Subsequently, the infected cells were treated with either anti-CD44 antibody or immunoglobulin G (Ig G). Accumulation of lipid droplets was visualized by Oil red O staining and protein levels were assayed by western blotting analysis. The results showed that sOPN and not rhOPN, notably increased the accumulation of lipid droplets and the expression of brown adipocyte-related genes. Moreover, neutralization of CD44 partially abrogated the effects induced by sOPN. These data demonstrate that sOPN and not rhOPN has the capacity to induce the differentiation of white preadipocytes into brown adipocytes through a CD44-dependent mechanism. The findings might provide a potential target for sOPN to combat obesity.

scholarly journals Embryonic cardiospecific knockout of α-E-catenin gene leads to alteration of energy metabolism in adult heart

2017 ◽  
Vol 23 (2) ◽  
pp. 65-69
Author(s):  
V. Balatskyy ◽  
L. Macewicz ◽  
O. Piven
Keyword(s):  
Lipid Metabolism ◽  
Energy Metabolism ◽  
Transgenic Mice ◽  
Lipid Droplets ◽  
Metabolic Disorders ◽  
Conditional Knockout ◽  
Heart Hypertrophy ◽  
Oil Red O Staining ◽  
Oil Red O

Previously we have shown that the α-E-catenin knockout in the embryonic heart leads to hypertrophy in adult and activation of canonical Wntsignaling. Heart hypertrophy is also accompanied by metabolic disorders, but role of the α-E-catenin in these processes is not known. Aim of our work is to study the effect of α-E-catenin deletion on the lipid metabolism in the heart. Methods. In our experiment we have used α-Е-catenin conditional knockout and αMHC-Cre transgenic mice. We have utilized histological (Oil Red O staining) and molecular biological (Western blot) methods. Results. α-Е-catenin deletion leads to accumulation of lipid droplets in myocardium, and to violation of expression and phosphorylation of key regulators of lipid metabolism (Ampk, Pparα, Acc, Hsl). Conclusions. Ous results suggest that α-Е-catenin deletion leads to inhibition of lipid metabolism in the heart.

scholarly journals Identification of a phenoloxidase- and melanin-dependent defence mechanism in Achatina fulica infected with Angiostrongylus vasorum

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Aytube Lucas Coaglio ◽  
Mônica Alves Neves Diniz Ferreira ◽  
Walter dos Santos Lima ◽  
Cíntia Aparecida de Jesus Pereira
Keyword(s):  
Angiostrongylus Vasorum ◽  
Achatina Fulica ◽  
Defence Mechanism

scholarly journals Evaluation on the phagocytosis of apoptotic spermatogenic cells by Sertoli cells in vitro through detecting lipid droplet formation by Oil Red O staining

Reproduction ◽  
2006 ◽  
Vol 132 (3) ◽  
pp. 485-492 ◽  
Author(s):  
Huizhen Wang ◽  
Haikun Wang ◽  
Weipeng Xiong ◽  
Yongmei Chen ◽  
Quanhong Ma ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Sertoli Cells ◽  
Lipid Droplets ◽  
Apoptotic Cell ◽  
Practical Method ◽  
Droplet Formation ◽  
Spermatogenic Cells ◽  
Lipid Droplet Formation ◽  
Oil Red O

During spermatogenesis, more than half of the differentiating spermatogenic cells undergo apoptosis before they mature into spermatozoa. Ultrastructure studies showed that the formation of lipid droplets in Sertoli cells was associated with phagocytosis of residual bodies and apoptotic germ cells by Sertoli cells. Here, a relationship between the phagocytosis of apoptotic spermatogenic cells and lipid droplet formation in Sertoli cells was studiedin vitroby Oil Red O (ORO) staining. The results confirmed that the formation of lipid droplets was a result of phagocytosis of apoptotic spermatogenic cells in Sertoli cells. By comparing phagocytosis of apoptotic spermatogenic cells and thymocytes by Sertoli cells to that by macrophages, we demonstrated that the lipid droplets accumulation in phagocytes depended on phagocytosed apoptotic cell type, but not phagocyte type. However, the size of lipid droplets was related to the type of phagocytes. By this approach, we found that Sertoli cells at different postnatal stages of development had a similar phagocytic ability. These results suggested that the detection of lipid droplets by ORO staining was a practical method to evaluate the phagocytic functions of Sertoli cellsin vitro. This approach could also be considered as anin vitromodel to study the lipid formation, metabolism, and function in Sertoli cells.

scholarly journals THE PRESENCE OF LIPID DROPLETS AND THE ABSENCE OF STABLE SUDANOPHILIA IN OSMIUM-FIXED HUMAN LEUKOCYTES

1971 ◽  
Vol 19 (9) ◽  
pp. 551-557 ◽  
Author(s):  
ANTONIO COIMBRA ◽  
A. LOPES-VAZ
Keyword(s):  
Lipid Droplets ◽  
Osmium Tetroxide ◽  
Myeloperoxidase Activity ◽  
Human Leukocytes ◽  
Sudan Black B ◽  
Free Lipid ◽  
Blood Smears ◽  
Human Granulocytes ◽  
Cold Acetone ◽  
Oil Red O

In blood smears fixed in osmium vapor and stained with Sudan black B or oil red O, neutrophil and eosinophil leukocytes contain a regular number of sudanophilic granules and do not exhibit stable sudanophilia. Stable sudanophilia occurs following aldehyde fixation without the appearance of sudanophilic granules in white cells. The sudanophilia in granules was lysochromic since the granules could be reversibly decolorized by dye solvents. Cold acetone extracted the content of the granules following osmium tetroxide oxidation. Electron microscopically the granules had the appearance of typical lipid droplets. Human granulocytes thus contain a constant amount of free lipid deposits that are adequately preserved only by osmium tetroxide fixation. Osmium seems to prevent the occurrence of stable sudanophilia through inhibition of myeloperoxidase activity. The procedure may be useful to follow variations of lipid content in human leukocytes, as exemplified by the excess of lipid found in leukocytes, in synovial fluids of patients with rheumatoid arthritis.

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