scholarly journals Search for sources of resistance to Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) in okra germplasm

2013 ◽  
Vol 13 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Frederick Mendes Aguiar ◽  
Sami Jorge Michereff ◽  
Leonardo Silva Boiteux ◽  
Ailton Reis
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Warm Season ◽  
Abelmoschus Esculentus ◽  
Santa Cruz ◽  
Sources Of Resistance ◽  
Inoculation Technique ◽  
Resistance Sources ◽  
Intermediate Resistance ◽  
Greenhouse Screening

Fusarium oxysporum f. sp. vasinfectum (FOV) is one the most destructive okra (Abelmoschus esculentus) pathogens in Brazil. Fifty-four okra accessions were evaluated for resistance to FOV. Greenhouse screening was initially carried out with one FOV isolate ('Fus-194'). Inoculation (in all assays) was carried out with 21-day-old plantlets, using the root-dipping inoculation technique. Thirty-three accessions displaying differential responses in the first screening were re-evaluated in two additional assays, using two FOV isolates ('Fus-194' and 'Fus-201'). Twelve accessions were rated as highly to intermediately resistant to 'Fus-194' during the dry/moderate temperature season, whereas nine accessions were classified as highly to intermediately resistant to 'Fus-201'. In the assay carried out in the wet and warm season, 72% of the accessions were classified as having high and intermediate resistance to 'Fus-194', and 32% were resistant to 'Fus-201'. The accessions 'Santa Cruz-47', 'BR-2399' and 'BR-1449' were the most promising resistance sources.

scholarly journals Natural Variation in Portuguese Common Bean Germplasm Reveals New Sources of Resistance Against Fusarium oxysporum f. sp. phaseoli and Resistance-Associated Candidate Genes

2020 ◽  
Vol 110 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Susana T. Leitão ◽  
Marcos Malosetti ◽  
Qijan Song ◽  
Fred van Eeuwijk ◽  
Diego Rubiales ◽  
...  
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Candidate Genes ◽  
Fusarium Wilt ◽  
Functional Markers ◽  
Gene Pools ◽  
Primary Metabolism ◽  
Sources Of Resistance ◽  
Wilt Resistance ◽  
Fusarium Wilt Resistance

Common bean (Phaseolus vulgaris) is one of the most consumed legume crops in the world, and Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the major diseases affecting its production. Portugal holds a very promising common bean germplasm with an admixed genetic background that may reveal novel genetic resistance combinations between the original Andean and Mesoamerican gene pools. To identify new sources of Fusarium wilt resistance and detect resistance-associated single-nucleotide polymorphisms (SNPs), we explored, for the first time, a diverse collection of the underused Portuguese common bean germplasm by using genome-wide association analyses. The collection was evaluated for Fusarium wilt resistance under growth chamber conditions, with the highly virulent F. oxysporum f. sp. phaseoli strain FOP-SP1 race 6. Fourteen of the 162 Portuguese accessions evaluated were highly resistant and 71 intermediate. The same collection was genotyped with DNA sequencing arrays, and SNP–resistance associations were tested via a mixed linear model accounting for the genetic relatedness between accessions. The results from the association mapping revealed nine SNPs associated with resistance on chromosomes Pv04, Pv05, Pv07, and Pv08, indicating that Fusarium wilt resistance is under oligogenic control. Putative candidate genes related to phytoalexin biosynthesis, hypersensitive response, and plant primary metabolism were identified. The results reported here highlight the importance of exploring underused germplasm for new sources of resistance and provide new genomic targets for the development of functional markers to support selection in future disease resistance breeding programs.

scholarly journals Potential Sources of Resistance to Fusarium Wilt and Powdery Mildew in Melons

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1657-1660 ◽  
Author(s):  
José María Alvarez ◽  
Rafael González-Torres ◽  
Cristina Mallor ◽  
María Luisa Gómez-Guillamón
Keyword(s):  
Powdery Mildew ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Cucumis Melo ◽  
Wild Relatives ◽  
Natural Resistance ◽  
Sphaerotheca Fuliginea ◽  
Sources Of Resistance ◽  
Potential Sources ◽  
Artificial Inoculations

In total, 139 Cucumis melo accessions were evaluated for resistance to races 0, 1, and 2 of Fusarium oxysporum fsp. melonis and 127 accessions were evaluated for resistance to races 1 and 2 of Sphaerotheca fuliginea. In addition, seven C. melo wild relatives were also tested. Artificial inoculations were performed and plants were scored for presence or absence of symptoms. The screening revealed that sources of natural resistance to these fungi are limited. However, several sources of resistance were found in C. melo accessions. Thus, the accession `CUM-334' from Tajikistan has shown resistance to the three races of F. oxysporum fsp. melonis, behaving similarly to the melon inbred line `MR1'. Two accessions of C. melo var. conomon, `CUM-190' and `Shiroubi Okayama', from Japan, were resistant to races 0 and 1 and twelve accessions were resistant to races 0 and 2. Intra-specific variability for resistance to powdery mildew in C. melo was found to be poor. Nevertheless, six Spanish cultivars and the accessions `TGR-1551', `CUM-313', and `CUM-129' were resistant to races 1 and 2 of S. fuliginea.

scholarly journals Pea Fusarium wilt races in western Algeria

2014 ◽  
Vol 50 (No. 2) ◽  
pp. 70-77
Author(s):  
A. Merzoug ◽  
L. Belabid ◽  
M. Youcef-Benkada ◽  
F. Benfreha ◽  
B. Bayaa
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Environmental Conditions ◽  
Yield Losses ◽  
Sources Of Resistance ◽  
Climatic Zones ◽  
Pathogen Variability ◽  
Western Algeria ◽  
Differential Hosts ◽  
Pathogenic Variability

The fungus Fusarium oxysporum f.sp. pisi (FOP), the pea wilt pathogen, causes appreciable yield losses under favourable environmental conditions in Algeria. Studies on the pathogen variability and distribution of races are essential to identify effective sources of resistance to this disease. In this study, a survey was conducted during the period 2007–2011 in four different agro-climatic zones. Pathogenic variability in 52 isolates of FOP, collected from different pea-growing areas of western Algeria, were evaluated using 7 genotypes as differential hosts. Results indicated that the disease was prevalent in all fields prospected and isolates obtained were assigned to Races 1, 2, 5 or 6 by their pathogenicity. It was found out that Races 1 and 2 were more common in all areas with 61.5 and 19.2%, respectively. This study is the first report of pea Fusarium wilt races distribution in Algeria.  

scholarly journals Novel Sources of Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 Among Solanum pennellii Accessions

2022 ◽  
Vol 147 (1) ◽  
pp. 35-44
Author(s):  
Jian Li ◽  
Jessica Chitwood-Brown ◽  
Gurleen Kaur ◽  
Joanne A. Labate ◽  
G.E. Vallad ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Resistance Genes ◽  
Genotyping By Sequencing ◽  
Sources Of Resistance ◽  
Solanum Pennellii ◽  
Tomato Production ◽  
Wild Tomato Species ◽  
Tomato Species

Fusarium wilt of tomato (Solanum lycopersicum), caused by fungal pathogen Fusarium oxysporum f. sp. lycopersici (Fol), is one of the most important diseases in tomato production. Three races of the pathogen are described, and race-specific resistance genes have been applied in commercial tomato cultivars for controlling the disease. Race 3 (Fol3) threatens tomato production in many regions around the world, and novel resistance resources could expand the diversity and durability of Fol resistance. The wild tomato species, Solanum pennellii, is reported to harbor broad resistance to Fol and was the source of two known Fol3 resistance genes. In this study, we evaluated 42 S. pennellii accessions for resistance to each fusarium wilt race. F1 plants, developed from crossing each accession with the Fol3 susceptible line ‘Suncoast’, were evaluated for Fol3 resistance, and BC1F1 plants were screened to determine the likelihood that Fol3 resistance was based on a novel locus (loci). Nearly all accessions showed resistance to Fol3, and many accessions were resistant to all races. Evaluation of F1 plants indicated a dominant resistance effect to Fol3 from most accessions. Genetic analysis indicated 24 accessions are expected to contain one or more novel Fol3 resistance loci other than an allele near the I-3 locus. To investigate genetic structure of the S. pennellii accessions used in this study, we genotyped all 42 accessions using genotyping by sequencing. Approximately 20% of the single nucleotide polymorphism (SNP) loci were heterozygous across accessions, likely due to the outcrossing nature of the species. Genetic structure analysis at 49,120 unique SNP loci across accessions identified small but obvious genetic differentiations.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

scholarly journals QTL Mapping Identifies Novel Source of Resistance to Fusarium Wilt Race 1 in Citrullus amarus

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 984-989 ◽  
Author(s):  
Sandra E. Branham ◽  
Amnon Levi ◽  
W. Patrick Wechter
Keyword(s):  
Fusarium Wilt ◽  
Citrullus Lanatus ◽  
Recombinant Inbred Lines ◽  
The United States ◽  
Chromosome 9 ◽  
Mapping Study ◽  
Sources Of Resistance ◽  
Race 1 ◽  
Genetic Source ◽  
Genetic Mapping Study

Fusarium wilt race 1, caused by the soilborne fungus Fusarium oxysporum Schlechtend.: Fr. f. sp. niveum (E.F. Sm.) W.C. Snyder & H.N. Hans (Fon), is a major disease of watermelon (Citrullus lanatus) in the United States and throughout the world. Although Fusarium wilt race 1 resistance has been incorporated into several watermelon cultivars, identification of additional genetic sources of resistance is crucial if a durable and sustainable level of resistance is to be continued over the years. We conducted a genetic mapping study to identify quantitative trait loci (QTLs) associated with resistance to Fon race 1 in segregating populations (F2:3 and recombinant inbred lines) of Citrullus amarus (citron melon) derived from the Fon race 1 resistant and susceptible parents USVL246-FR2 and USVL114, respectively. A major QTL (qFon1-9) associated with resistance to Fon race 1 was identified on chromosome 9 of USVL246-FR2. This discovery provides a novel genetic source of resistance to Fusarium wilt race 1 in watermelon and, thus, an additional host-resistance option for watermelon breeders to further the effort to mitigate this serious phytopathogen.

Sign in / Sign up

Export Citation Format

Share Document