PERIPHERAL CONCENTRATIONS OF PROGESTERONE, CORTISOL, ALDOSTERONE, SODIUM AND POTASSIUM IN THE PLASMA OF THE TADMIT EWE DURING PREGNANCY AND PARTURITION

1980 ◽  
Vol 84 (1) ◽  
pp. 25-33 ◽  
Author(s):  
LOUISA BOULFEKHAR ◽  
ROGER BRUDIEUX
Keyword(s):  
Early Pregnancy ◽  
Competitive Inhibition ◽  
Plasma Aldosterone ◽  
Renin Substrate ◽  
Sodium Potassium ◽  
Blood Concentrations ◽  
Plasma Progesterone ◽  
Mean Values ◽  
The Mean ◽  
Sodium And Potassium

The peripheral blood concentrations of progesterone, cortisol, aldosterone and electrolytes were measured simultaneously in 16 multiparous ewes of the Tadmit Algerian breed during pregnancy and parturition. Plasma progesterone concentrations were similar to those of the luteal phase of the oestrous cycle during the first third of gestation. Between 60 and 135 days after mating, the levels of plasma progesterone increased steadily to values eight times those found in early pregnancy. However, no change was observed from days 43 to 28 before parturition. Mean progesterone concentrations declined during the 17 days preceding lambing, decreasing more quickly during the last 3 days. Pregnancy did not increase the levels of plasma cortisol and the mean values during pregnancy did not exceed 0·5 μg/100 ml until 90 days after mating. It then tended to decrease between days 57 and 17 before parturition, before rising slightly on both day 7 and on the day of lambing. Concentrations of plasma aldosterone were low during early pregnancy (about 2 ng/100 ml). Thereafter, as with progesterone, they rose from days 58 to 43, did not change from days 43 to 28 and increased again until maximum levels were reached 17 days prepartum and then levels decreased sharply until 7 days before lambing. At this stage, progesterone levels continued to decrease during the last 7 days while aldosterone concentrations once more increased. Sodium and potassium concentrations changed little during pregnancy, although the sodium: potassium ratio tended to increase during the last 17 days of pregnancy. It is suggested that changes in the levels of plasma aldosterone during gestation in the ewe are the result of the competitive inhibition of aldosterone by progesterone at a renal level and that the rise of aldosterone during the last few days of pregnancy is largely due to the stimulation of renin substrate production by oestrogens whose values are high at this period.

scholarly journals Metabolic evaluation of Crioulo horses participating in competitions of 750 km

2013 ◽  
Vol 33 (12) ◽  
pp. 1471-1477 ◽  
Author(s):  
Lorena Alvariza Amaral ◽  
Millie Marchiori ◽  
Charles Ferreira Martins ◽  
Marcio Nunes Correa ◽  
Carlos Eduardo Wayne Nogueira
Keyword(s):  
Inflammatory Process ◽  
Ionized Calcium ◽  
Metabolic Responses ◽  
Total Calcium ◽  
Blood Cell Count ◽  
Metabolic Evaluation ◽  
Sodium Potassium ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean

The Crioulo breed of horses performs in one of the most physically demanding equestrian competitions, the Marcha de Resistência, which is a contest in which the horses run 750 km in 15 days. The study's aim was to characterize the metabolic responses during this period. We evaluated eleven Crioulo horses in the competition, specifically, two males and nine females. Blood samples were collected 24 hours before the contest and on the 4th, 9th, 11th, 14th and 15th days of competition. We evaluated CK, AST, LDH, glucose, lactate, urea, creatinine, sodium, potassium, chloride, magnesium, total calcium, ionized calcium, total protein, hematocrit and the white blood cell count. At the end of the competition, the mean values of serum AST were 1151±358 IU/ L the mean LDH values were 7418±1695 IU/L and CK was 13,867±3998UI /L. There was a significant increase in urea, creatinine and lactate (p<0.0001). A decrease in the mean values of chloride, sodium, potassium, and total and ionized calcium was observed (p≤0.0002). An evaluation of the total leukocytes and segmented neutrophils (p≤0.0002) revealed their increased values, and decreased values were observed for hematocrit, plasma protein and total lymphocytes (p≤0.0003). The values of glucose, on average, remained constant. Based on these data, we conclude that the Marcha de Resistência competition necessitated a high muscular demand and the depletion of energy and electrolytes, suggesting an inflammatory process in the animals evaluated.

Biological variation in analyte concentrations in urine of apparently healthy men and women.

1987 ◽  
Vol 33 (6) ◽  
pp. 847-850 ◽  
Author(s):  
E M Gowans ◽  
C G Fraser
Keyword(s):  
Reference Values ◽  
Interindividual Variation ◽  
Healthy Individuals ◽  
Sodium Potassium ◽  
Men And Women ◽  
Mean Values ◽  
Urine Creatinine ◽  
Sodium And Potassium ◽  
Urine Specimens ◽  
Apparently Healthy

Abstract Analytical, intra-individual, and interindividual components of variation for sodium, potassium, urea, creatinine, calcium, and phosphate were estimated from results for 24-h urine specimens collected from 15 apparently healthy individuals every four weeks for 40 weeks. Expressed as output, mean values differed for men and women, except for calcium. Our data on intra-individual variation were similar to those obtained for 10 men by Shephard et al. (Clin Chem 1981;27:569-73). Calculated analytical goals are easily attained by current methods. Reference values for urine creatinine are useful only when expressed as output and stratified according to gender. The ratios of intra- to interindividual variation generally increase on such stratification; separate reference values for men and women are therefore required for analytes expressed as output. Measurements of sodium and potassium in urine should be reported as concentration, but output terms are favored for the other analytes. Differences for two serial results from an individual must be rather large to differ statistically.

HOURLY FLUCTUATIONS IN THE BLOOD LEVELS OF MELATONIN, PROLACTIN, LUTEINIZING HORMONE, FOLLICLE-STIMULATING HORMONE, TESTOSTERONE, TRI-IODOTHYRONINE, THYROXINE AND CORTISOL IN RAMS UNDER ARTIFICIAL PHOTOPERIODS, AND THE EFFECTS OF CRANIAL SYMPATHECTOMY

1982 ◽  
Vol 92 (2) ◽  
pp. 237-250 ◽  
Author(s):  
G. A. LINCOLN ◽  
O. F. X. ALMEIDA ◽  
H. KLANDORF ◽  
R. A. CUNNINGHAM
Keyword(s):  
Follicle Stimulating Hormone ◽  
Time Of Day ◽  
Blood Levels ◽  
Blood Concentrations ◽  
Blood Samples ◽  
Mean Values ◽  
Change In The Mean ◽  
The Mean ◽  
Cervical Ganglia ◽  
Short Days

Eight adult Soay rams (four control and four cranially sympathectomized by removal of the superior cervical ganglia) were exposed to alternating 16-week periods of short days of 8 h light: 16 h darkness (8L : 16D) and long days (16L : 8D) for more than 3 years, and the changes in the diameter of the testes were recorded. Once during short days and once during long days blood samples were collected hourly for 28 h, and the concentrations of melatonin, prolactin, FSH, LH, testosterone, tri-iodothyronine (T3), thyroxine (T4) and cortisol were measured by radioimmunoassay. In the control rams the testes were reduced in size during long days compared to short days, and the blood concentrations (24-h mean values) of FSH, LH and testosterone were decreased. The levels of prolactin were much increased, while there was no significant change in the mean levels of melatonin, T3, T4 and cortisol. During both photoperiods there was considerable hour-to-hour variation in all eight hormones indicative of episodic secretion, as well as a consistent variation related to the time of day which was most pronounced for melatonin and T3. There was a clear difference in the daily profile of plasma melatonin levels between short and long days. In the superior cervical ganglionectomized rams there were no significant changes in the size of the testes or in the hormone titres between short and long days. Compared to the controls the plasma levels of LH, FSH, testosterone and prolactin were in the intermediate range. Some consistent diurnal variation was evident in the levels of all the hormones measured, with a pattern similar to the controls for a few of the hormones (e.g. T3) but quite different for others (e.g. melatonin).

Studies on plasma progesterone concentrations and fertility in Friesland dairy cows during the post-partum period

1981 ◽  
Vol 97 (3) ◽  
pp. 649-654 ◽  
Author(s):  
D. H. Holness ◽  
G. W. Sprowson ◽  
Carole Sheward ◽  
Amanda Geel
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Post Partum ◽  
Ovarian Activity ◽  
Plasma Progesterone ◽  
Low Concentrations ◽  
The Mean ◽  
Post Partum Period ◽  
Embryo Loss

SUMMARYPlasma progesterone concentrations were monitored in 69 lactating Friesland dairy cows during the post-partum period. Cyclic ovarian activity resumed in most cows within 40 days of calving. Forty-eight per cent of cows remained pregnant to their first insemination, 41% returned to oestrus before day 30 after insemination, and a further 11% returned to oestrus between days 30 and 88 after insemination.The mean of progesterone values on days 11 to 4 before insemination tended to be higher in cows that conceived than in cows that did not conceive. These differences were signficant on day –8 (P <0·05) and on days –8, –7, –6 and –5 combined (P <0·01). Cows in which the concentration of progesterone in plasma did not rise above 4·5 ng/ml during this period did not conceive. After insemination, mean progesterone concentrations between pregnant and non-pregnant cows diverged after day +8. Relatively low concentrations of plasma progesterone (< 1 ng/ml) up to day +8 after insemination did not preclude conception. There was no indication that embryo loss between days +30 and +88 after insemination was related to an insufficiency of ovarian progesterone during early pregnancy.

Evaluation of serum electrolytes measurement through the 6-year trueness verification program in China

2021 ◽  
Vol 59 (1) ◽  
pp. 107-116
Author(s):  
Ying Yan ◽  
Yungang Pu ◽  
Jie Zeng ◽  
Tianjiao Zhang ◽  
Weiyan Zhou ◽  
...  
Keyword(s):  
Peer Group ◽  
Heterogeneous Systems ◽  
Pass Rates ◽  
Serum Samples ◽  
Sodium Potassium ◽  
Mean Values ◽  
Clinical Laboratories ◽  
Reference Methods ◽  
The Mean ◽  
Calcium And Magnesium

AbstractObjectivesThe National Center for Clinical Laboratories (NCCL) in China initiated a serum electrolyte trueness verification (ETV) program in 2014 for measurement standardization.MethodsEvery year, two levels of fresh frozen commutable serum samples determined by inductively coupled plasma mass spectrometry (ICP-MS) reference methods were transported to participating clinical laboratories for the measurement of sodium, potassium, calcium and magnesium. Both samples were measured 15 times in 3 days, and the mean values and coefficient variations (CVs) were calculated from the results. The tolerance limits of trueness (bias), precision (CV) and accuracy (TE) based on the biological variation database were used as the evaluation criteria. The overall trend of the ETV program over 6 years was surveyed by calculating the pass rates of the participating laboratories. The mean bias, inter-laboratory CV, and TE of all laboratory results were analysed. Furthermore, homogeneous and heterogeneous systems were compared, and the bias and CV results of mainstream analysis systems were analysed.ResultsPass rates of the three quality specifications increased, and the overall mean bias and inter-laboratory CVs decreased. The homogeneous system was superior to the heterogeneous system for calcium and magnesium measurements. For sodium, potassium, calcium and magnesium, the minimum bias corresponded to Hitachi, Siemens, Beckman AU and Roche, respectively. For inter-laboratory robust CVs, no obvious differences were observed between each peer group.ConclusionsThe commutable ETV materials assigned via reference methods can evaluate the accuracy and reproducibility of an individual laboratory and the calibration traceability and uniformity between laboratories for measurements.

A STUDY OF PLASMA VALUES OF SODIUM, POTASSIUM, CHLORIDE, CARBON DIOXIDE, CARBON DIOXIDE TENSION, SUGAR, UREA AND THE PROTEIN BASEBINDING POWER, pH AND HEMATOCRIT IN PREMATURES ON THE FIRST DAY OF LIFE

PEDIATRICS ◽  
1956 ◽  
Vol 18 (1) ◽  
pp. 39-49
Author(s):  
J. B. Pincus ◽  
I. F. Gittleman ◽  
M. Saito ◽  
A. E. Sobel
Keyword(s):  
Carbon Dioxide ◽  
Potassium Chloride ◽  
Chemical Constituents ◽  
Carbon Dioxide Tension ◽  
Weight Group ◽  
Sodium Potassium ◽  
Mean Values ◽  
The Mean ◽  
Binding Power ◽  
Mean Concentration

Seventy-three premature infants were studied on the first day of life before fluid therapy or feedings were begun. These were divided into 3 groups according to weight. A fourth group was included in this study as a "control." The following chemical constituents of the plasma were measured: sodium, potassium, chloride, CO2 content, total protein, urea and sugar. The blood pH and the hematocrit were also determined. The protein base-binding power was calculated. The observations were made on capillary blood obtained by heel puncture. Ultramicro-methods were used for the estimation of the various chemical constituents. The mean concentration of potassium is significantly higher in the lowest weight group; the protein and correspondingly the base-binding power of the protein is also significantly lower in this group. The mean values for sodium, chloride, CO2, pH, sugar and urea did not vary significantly with the birth weights of the infants. While the lowest pH values were encountered in the lowest weight group, the mean did not significantly differ from group to group. The mean concentration of urea was higher in the lowest weight group but did not significantly vary with the birth weight of the infants. The mean concentration of glucose in the plasma was lowest in the lowest weight group. The hematocrit values were uniformly above normal in all groups, but the mean did not vary from group to group.

Effect of estrus synchronization on plasma progesterone profile and fertility response in postpartum suckled anestrous Kankrej cows

2016 ◽  
Author(s):  
Mehrajuddin Naikoo ◽  
A. J. Dhami ◽  
Ammu Ramakrishnan
Keyword(s):  
Control Group ◽  
Ovarian Activity ◽  
Treatment Protocols ◽  
Plasma Progesterone ◽  
Mean Values ◽  
Cyclic Groups ◽  
Early Postpartum ◽  
Conception Rates ◽  
Cyclic Control ◽  
The Mean

The study was designed at evaluating early postpartum ovarian activity through plasma progesterone profile at 10 days interval up to 90 days, followed by induction of estrus in postpartum anestrous suckled Kankrej cows (n=18) using Ovsynch, CIDR and Ovsynch + CIDR treatment protocols with FTAI (6 cows in each protocol), keeping six normal cyclic cows as control. The plasma P4 profile (ng/ml) varied in the range of 0.15±0.03 to 1.70±0.80 in treatment groups and 0.62 ±0.19 to 4.09±1.60 in control group up to 90 days postpartum. The mean P4 concentration on the day of calving was low (<1 ng/ml) in all the groups, which gradually increased to reach peak levels by day 40 in cyclic control (4.09±1.60 ng/ml), Ovsynch (1.70±0.80 ng/ml) and CIDR (1.68±0.47 ng/ml) and by day 20 in Ovsynch + CIDR (1.09±0.23 ng/ml) groups. Most of the cows turned into subestrous or anestrous condition between days 40 and 90 postpartum. The estrus induction response in Ovsynch, CIDR and Ovsynch + CIDR groups initiated at day 90-92 postpartum was 66.66, 83.33 and 50.00 per cent, respectively. The conception rates at induced estrus were 16.66, 33.33 and 16.66 per cent, and overall of 3 cycles 33.33 (2/6), 50.00 (3/3) and 33.33 (2/6) per cent, respectively. In normal cyclic control group, the conception rates at first cycle and overall of 3 cycles were 33.33 and 50.00 per cent. The mean plasma P4 (ng/ml) concentrations were significantly (P<0.05) higher on day 7 in Ovsynch (1.41 ±0.10), CIDR (4.92±0.83) and Ovsynch + CIDR (3.87±0.84) protocols as compared to their corresponding values on day 0 and day 9/10 (AI). The mean values of plasma P4 from days 20 to 40 post-AI were higher in conceived cows than in non-conceived cows of CIDR and Ovsynch + CIDR groups, compared to the animals in Ovsynch and normal cyclic groups. It is opined that the use of different protocols, mainly CIDR, may serve as an effective tool for induction and synchronization of estrus and improvement of conception rate in postpartum anestrous suckled Kankrej cows.

Renal and adrenal responses to [des-Asp1]angiotensin I in the dog

1978 ◽  
Vol 234 (2) ◽  
pp. F130-F134 ◽  
Author(s):  
R. H. Freeman ◽  
J. O. Davis ◽  
M. C. Khosla
Keyword(s):  
Blood Flow ◽  
Renal Blood Flow ◽  
Bolus Injection ◽  
Enzyme Inhibitor ◽  
Plasma Aldosterone ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Plasma Aldosterone Concentration ◽  
The Mean ◽  
Sodium And Potassium

There is evidence for the endogenous generation of [des-Asp1]angiotensin II (AIII) from a nonapeptide precursor, [des-Asp1]angiotensin I ([des-Asp1]AI). In the present study, the effects of equipressor doses of exogeneously administered [des-Asp1]AI and AIII on renal function and plasma aldosterone concentration were compared. Intravenous infusion of [des-Asp1]AI (75 ng/kg min-1 for 40 min) decreased renin secretion, renal blood flow, creatinine clearance, and sodium and potassium excretion in dogs. Infusion of AIII at one-third of the rate of [des-Asp1]AI (25 ng/kg min-1) produced comparable decreases in these same parameters. Filtration fraction was increased with both peptides. Both peptides also increased plasma aldosterone concentration to the same extent. A bolus injection (5 mg i.v.) of the converting enzyme inhibitor SQ 20,881 completely reversed the mean arterial pressure and renal blood flow responses to [des-Asp1]AI, but did not alter these responses to AIII. These data are consistent with the concept that endogenous generation of AIII from [des-Asp1]AI can occur via the action of converting enzyme on this substance.

EFFECTS OF NORETHISTERONE ON PITUITARY AND OVARIAN FUNCTION

1974 ◽  
Vol 75 (2) ◽  
pp. 357-367 ◽  
Author(s):  
W. P. Collins ◽  
E. N. Koullapis ◽  
K M. Ferguson ◽  
S. Biswas ◽  
M. J. Sharples ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Urinary Metabolites ◽  
Plasma Progesterone ◽  
Mean Values ◽  
Control Value ◽  
Progesterone Metabolism ◽  
Cyclical Pattern ◽  
Variable Reduction ◽  
The Mean ◽  
17 Hydroxyprogesterone

ABSTRACT The effect of norethisterone (17α-ethynyl-17β-hydroxy-19-norandrost-4-en-3-one) on certain aspects of pituitary and ovarian function has been investigated in seven healthy women. Twenty-four urine samples were collected daily throughout control cycles and subsequently during the second cycle of treatment with 0.35 mg/day of norethisterone. LH, FSH and total oestrogens were measured in all urine specimens and the level of pregnanediol-3α-glucuronoside on selected days during the luteal phase. In addition, progesterone, 17-hydroxyprogesterone and oestradiol were determined in peripheral venous plasma nine days after the peak of urinary LH. At the same time, progesterone secretion and metabolism were assessed from the analyses of urinary metabolites following the intravenous administration of labelled progesterone. The results show that, in general, norethisterone at this dose does not affect either the level or pattern of FSH, but in five subjects, there was a variable reduction in the midcycle peak of LH (to 33–85 % of the control value). In six subjects, there was a marked reduction in the levels of urinary pregnanediol (to 8–80% of the control value), plasma progesterone (16–55 %), and the urinary production rate of progesterone (3–51 %). In contrast, the level of urinary oestrogen per cycle was increased to 140–160 % in all subjects and the mean values showed an abnormal cyclical pattern. Changes in the distribution of labelled metabolites during treatment indicated the existence of at least two pools of progesterone metabolism.

Studies on the mechanism by which ACTH stimulates renin activity and angiotensin II formation in man

1982 ◽  
Vol 100 (4) ◽  
pp. 573-580 ◽  
Author(s):  
Wolfgang Oelkers ◽  
Angelika Köhler ◽  
Lutz Belkien ◽  
Regina Fuchs-Hammoser ◽  
Mahamane Maiga ◽  
...  
Keyword(s):  
Adrenergic Receptors ◽  
Plasma Cortisol ◽  
Plasma Renin ◽  
Early Morning ◽  
Plasma Aldosterone ◽  
Renin Activity ◽  
Electrolyte Balance ◽  
Renin Substrate ◽  
Sodium Potassium ◽  
Normal Men

Abstract. Ten IU of ACTH (1–24) per day was infused for 34 h (starting at 7 a.m.) into 8 normal men on a constant diet containing 135 mm Na+ per day. All subjects retained between 152 and 181 mm of sodium. Potassium balance was negative. Plasma renin activity (PRA) and plasmaangiotensin II (P-A II) started to rise in most subjects after 6 to 8 h of infusion, reached a maximum after 24 h and then tended to decline. As shown previously, the rise in PRA is not due to a rise in plasma renin substrate concentration. Systolic, but not diastolic blood pressure increased significantly on the second day of ACTH-infusion. Plasma cortisol (P-F) was continuously stimulated by ACTH. Plasma aldosterone (P-aldo) increased rapidly 1 h after ACTH administration, then tended to fall, and increased again in most subjects, roughly in parallel with PRA. No significant changes in electrolyte balance, PRA, P-A II, P-F and P-aldo occurred in 3 subjects receiving 'sham'-infusions. Additional experiments in subjects treated with propranolol or indomethacin allowed the conclusion that the effect of ACTH on PRA and P-A II is not mediated by renal beta-adrenergic receptors, but perhaps (partially?) by prostaglandins. Since the infusion rate of ACTH was not much higher than the secretion rate of ACTH in the early morning hours, it is possible that ACTH is physiologically involved in the regulation of renin secretion.

Sign in / Sign up

Export Citation Format

Share Document