Oestriol and non-protein-bound oestriol concentrations in human peripheral plasma before labour and delivery

1986 ◽  
Vol 108 (1) ◽  
pp. 75-80 ◽  
Author(s):  
V. Moutsatsou ◽  
R. E. Oakey
Keyword(s):  
Plasma Concentration ◽  
Plasma Protein ◽  
Plasma Samples ◽  
Individual Subject ◽  
Peripheral Plasma ◽  
Uncomplicated Pregnancy ◽  
Apparent Concentration ◽  
The Mean ◽  
Mean Concentration ◽  
Centrifugal Ultrafiltration

ABSTRACT The concentration of oestriol and the proportion of this hormone not bound to plasma protein were measured using radioimmunoassay and centrifugal ultrafiltration respectively, in 55 samples of plasma obtained from 12 women in the last 2 to 7 weeks of uncomplicated pregnancy. Among individuals, the mean plasma concentration of oestriol varied from 25·8 to 94·8 nmol/l; in nine subjects, there was a tendency for oestriol concentrations to increase as delivery approached. The mean proportion of oestriol not bound to plasma protein in the different subjects varied from 13·1 to 18·9%, but values from any individual subject remained essentially constant during the periods of study. These measured values were used to calculate, for each sample, the apparent concentration of oestriol not bound to plasma protein. The results were combined with analogous values for oestradiol and progesterone obtained from the same plasma samples and described in a previous study. It was found that (i) the mean ratio of the concentration of oestriol and oestradiol was 0·75, (ii) the mean concentration of non-protein-bound oestriol was 8·7 times that of non-protein-bound oestradiol, and (iii) in individual subjects, there was no consistent trend as delivery approached in the ratio of the concentration of progesterone to that of oestriol in either the total or non-protein-bound form. J. Endocr. (1986) 108, 75–80

Non-protein-bound oestradiol and progesterone in human peripheral plasma before labour and delivery

1985 ◽  
Vol 104 (1) ◽  
pp. 7-15 ◽  
Author(s):  
P. J. B. Anderson ◽  
K. W. Hancock ◽  
R. E. Oakey
Keyword(s):  
Steady State ◽  
Peripheral Blood ◽  
Experimental Error ◽  
Gel Filtration ◽  
Peripheral Circulation ◽  
Plasma Samples ◽  
Peripheral Plasma ◽  
Human Labour ◽  
Apparent Concentration ◽  
The Mean

ABSTRACT Plasma samples were obtained at weekly intervals from the peripheral circulation of 12 women in the last 2–7 weeks of pregnancy. The concentrations of oestradiol and progesterone (isolated by chromatography) were measured by radioimmunoassay; the proportion of each hormone which was not bound to protein was measured by steady-state gel filtration. From these, the apparent concentration of the non-protein-bound form of each hormone was calculated. The mean proportion of oestradiol not bound to protein varied from 0·84 to 2·71% in the different subjects, but within each subject variation was within experimental error. For progesterone, the mean proportion not bound to protein in the different subjects varied from 1·76 to 2·77%; within individuals the proportion remained essentially constant. There was no consistent, recognizable trend as labour approached in (i) the concentration of oestradiol; (ii) the concentration of progesterone; (iii) the concentrations of non-protein-bound oestradiol or non-protein-bound progesterone; (iv) the ratio of the concentrations of progesterone and oestradiol; (v) the ratio of the concentrations of non-protein-bound progesterone and oestradiol. In nine out of 12 subjects, the ratio of the concentration of non-protein-bound progesterone to that of non-protein-bound oestradiol was greater than the corresponding ratio based on total hormone concentrations. These results therefore provide no support for the hypothesis that human labour is preceded by alteration in the progesterone to oestradiol ratio which can be detected by measurement of these hormones in peripheral blood. J. Endocr. (1985) 104, 7–15

Hormonal changes in post-partum blood plasma and milk of buffaloes (Bubalus bubalis)

1983 ◽  
Vol 37 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G. S. Pahwa ◽  
R. S. Pandey
Keyword(s):  
Plasma Concentration ◽  
Blood Plasma ◽  
Post Partum ◽  
Bubalus Bubalis ◽  
Complete Regression ◽  
Hormonal Changes ◽  
Similar Concentration ◽  
The Mean ◽  
A Minor ◽  
Mean Concentration

ABSTRACTPost-partum changes in the concentration of progesterone, oestradiol-17β and prolactin were measured by radioimmunoassay in the blood plasma and milk of 28 buffaloes. The mean concentration of progesterone in the plasma of normal animals was 0·24 ± 0·04 μ.g/1 on the day of calving and decreased linearly during the post-partum period, until the complete regression of the residual corpus luteum of pregnancy occurred, when the plasma concentration was recorded as 0μ05 to 0μ08 μg/l. The concentration at a silent oestrus was 0μ17 ± 0·02 μ.g/1. The milk progesterone profile was parallel to that of plasma but the level was 2 to 4 times higher.The mean concentration of oestradiol-17β in plasma was 41·0 ± 2·34 ng/1 on the day of calving; it declined significantly by day 1 post partum (P < 0·001) and fluctuated between 8 and 16 ng/1 until oestrus, when the concentration was approximately 25 ng/1. The oestradiol-17β concentration in milk was significantly higher than that of plasma on the day of calving. During the first 20 days post partum, a similar concentration of oestradiol-17β was maintained in both fluids but thereafter the concentration in milk was 2 to 3 times that of plasma.The mean concentration of prolactin in plasma was 212·6± 19·08 μg/1 on the day of calving and then declined and fluctuated between 70 and 270 μg/1 during the rest of the period. A minor elevation occurred around oestrus. The prolactin concentration for the first 2 days post partum in milk was significantly higher than that in plasma but during the rest of the period it was similar to that in plasma.The progesterone concentrations were slightly lower in anoestrous animals than in those cycling. The concentration of prolactin was significantly higher in multiparous animals (P < 0·001), whilst the progesterone and oestradiol values were similar in primiparous and multiparous animals.

THE PROGESTERONE CONCENTRATION IN THE PLASMA OF THE GOAT DURING THE OESTROUS CYCLE AND PREGNANCY

1972 ◽  
Vol 52 (1) ◽  
pp. 23-36 ◽  
Author(s):  
G. D. THORBURN ◽  
W. SCHNEIDER
Keyword(s):  
Binding Assay ◽  
Progesterone Concentration ◽  
Maternal Circulation ◽  
Plasma Progesterone ◽  
Pregnant Uterus ◽  
Main Site ◽  
Peripheral Plasma ◽  
Protein Binding Assay ◽  
The Mean ◽  
Mean Concentration

SUMMARY Progesterone concentrations in the peripheral plasma of goats were measured by a protein-binding assay. The mean concentration was extremely low on the day of oestrus (0·2 ng/ml) and was not significantly different from that found in anoestrous or ovariectomized animals. The concentration increased to a maximum of 4 ng/ml on about day 10 of the 21-day cycle, and decreased rapidly during the last 3 days of the cycle. Plasma progesterone concentration during early pregnancy (2·5–3·5 ng/ml) was similar to the luteal phase value and remained steady from day 8 to day 60. Between days 60 and 70 there was a secondary increase in progesterone concentration which was maintained at this increased level (4·5–5·5 ng/ml) until just before parturition. In twin-bearing animals, the secondary increase was greater. Progesterone concentration decreased rapidly during the 1–2 days preceding parturition, but the concentration was still quite high on the day of parturition (1·25 ng/ml). The progesterone concentration in peripheral plasma was markedly increased during anaesthesia and the operation. After bilateral ovariectomy of the pregnant goat, peripheral progesterone concentration fell rapidly from 9 to 2·5 ng/ml during the first ½ h and then more slowly during the next 5–6 h. The animals aborted 36–48 h later. A consistent positive arterio—venous difference for progesterone was observed across the pregnant uterus in two unanaesthetized goats. These results indicate that the ovary is the main site of progesterone production in the pregnant goat and that production by the placenta is small and unlikely to influence the level of this hormone in the maternal circulation.

Improved radioimmunoassay of atrial natriuretic peptide in plasma.

1989 ◽  
Vol 35 (7) ◽  
pp. 1431-1434 ◽  
Author(s):  
C Poiesi ◽  
A Rodella ◽  
G Mantero ◽  
G Cannella ◽  
R Ferrari ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Healthy Volunteers ◽  
Natriuretic Peptide ◽  
Extraction Procedure ◽  
Plasma Samples ◽  
Standard Assay ◽  
One Step ◽  
The Mean ◽  
Mean Concentration ◽  
Atrial Natriuretic

Abstract We describe a radioimmunoassay (RIA) for measurement of atrial natriuretic peptide (ANP), based on one-step incubation and a simplified extraction procedure. The extraction was performed on a "Supelclean LC 18" column, with 2-mL plasma samples. Use of a diiodinated tracer improved the sensitivity of the RIA method. The minimal detectable value was 5 ng/L. Simplification of the extraction procedure and simultaneous incubation of the reagents provide a method more suitable for routine standard assay of ANP than those currently available. Intra- and interassay CVs were 6% (n = 12) and 11% (n = 10), respectively. The mean concentration of ANP in plasma of 32 healthy volunteers was 33 (SEM 4) ng/L. The ANP values for plasma after one-step incubation correlated well with those determined by a commercial RIA kit: r = 0.971, slope = 1.099, intercept = 1.949 ng/L (n = 25).

TESTOSTERONE AND OESTRADIOL IN DOGS WITH TESTICULAR TUMOURS

1974 ◽  
Vol 77 (2) ◽  
pp. 408-416 ◽  
Author(s):  
F. Comhaire ◽  
D. Mattheeuws ◽  
A. Vermeulen
Keyword(s):  
Plasma Concentration ◽  
Sertoli Cell ◽  
Sex Steroids ◽  
Venous Blood ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Normal Mass ◽  
The Mean ◽  
Sertoli Cell Tumours

ABSTRACT The mean peripheral plasma concentration of oestradiol was found to be increased in 3 dogs with Sertoli cell tumours and in 3 dogs with seminomas, whereas the plasma testosterone showed no difference as compared to a group of dogs without testicular neoplasia. In two thirds of the cases the concentration of oestradiol in the spermatic venous blood draining the neoplastic testes was clearly higher than in the normal dogs. The testosterone concentration in the spermatic venous blood from the tumour bearing testes was lower than in the spermatic venous blood of the contralateral partner testes in the same dogs, though not different from the spermatic venous concentration in the control dogs. There was no correlation between the presence or absence of signs of feminization and the peripheral or spermatic venous concentration of sex steroids. It is concluded that not only Sertoli cell tumours, but also seminomas can secrete increased amounts of oestrogens. This is possibly due to the presence of a larger than normal mass of tissue capable of converting testosterone, or its precursors, to oestradiol.

STIMULATORY EFFECT OF PROSTAGLANDIN F2α ON THE PLASMA CONCENTRATION OF TESTOSTERONE IN BULLS

1975 ◽  
Vol 66 (3) ◽  
pp. 329-338 ◽  
Author(s):  
N. B. HAYNES ◽  
H. D. HAFS ◽  
R. J. WATERS ◽  
J. G. MANNS ◽  
A. RILEY
Keyword(s):  
Plasma Concentration ◽  
Intramuscular Injection ◽  
Prostaglandin F2α ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
The Mean

SUMMARY The concentrations of peripheral plasma testosterone were measured by radioimmunoassay in samples collected from five bulls, each given i.m. injections of 0, 5, 15, 30 and 60 mg prostaglandin F2α (PGF2α). Synchronized peaks in testosterone concentration occurred with maximum values 1–2 h after treatment. These increases of testosterone persisted significantly longer than those observed to occur as natural episodic peaks during two 24 h periods in the same bulls. The mean peak testosterone concentration after PGF2α injection was related to the dose of PGF2α, values after 60 and 30 mg doses being significantly greater than after 15 mg. The response produced by a 5 mg dose was not significant. The results indicate that intramuscular injection of PGF2α acutely stimulates synthesis and release of testosterone in bulls.

Plasma concentrations of testosterone and luteinizing hormone in rutting reindeer bulls (Rangifer tarandrus)

1980 ◽  
Vol 58 (11) ◽  
pp. 2081-2083 ◽  
Author(s):  
K.-A. Stokkan ◽  
K. Hove ◽  
W. R. Carr
Keyword(s):  
Luteinizing Hormone ◽  
Testosterone Level ◽  
Plasma Concentrations ◽  
Plasma Testosterone ◽  
Plasma Samples ◽  
Diurnal Pattern ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
The Mean

Concentrations of plasma testosterone and luteinizing hormone (LH) were measured in peripheral plasma from semidomestic, rutting reindeer bulls. Although the concentrations of plasma testosterone were high and showed large variations, those of LH were low and only a few episodic bursts could be detected in hourly samples taken throughout a 48-h period. The mean testosterone concentration in three bulls differed significantly and ranked the animals according to their position in a fighting hierarchy. The mean concentrations of LH did not differ significantly. Plasma samples from one reindeer bull sampled every 20 min for periods of 3 h indicated that an increment in LH concentration preceded a peak in testosterone. No diurnal pattern in testosterone concentrations could be detected, but testosterone peaks seemed to appear about every 3–4 h. The present study thus demonstrates that a series of plasma samples throughout the day is necessary to determine a true "testosterone level" in the reindeer bull.

Is hirsutism an evolving syndrome?

1983 ◽  
Vol 97 (3) ◽  
pp. 379-387 ◽  
Author(s):  
Vincenzo Toscano ◽  
M. V. Adamo ◽  
Stefania Caiola ◽  
Sonia Foli ◽  
Elisa Petrangeli ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Static Condition ◽  
Dehydroepiandrosterone Sulphate ◽  
Acth Stimulation ◽  
Group V ◽  
Peripheral Plasma ◽  
Group I ◽  
Menstruating Women ◽  
The Mean ◽  
Mean Concentration

The possibility that hirsutism is an evolving syndrome rather than a static condition involving only one gland has been considered. To assess this proposal 60 untreated hirsute patients aged 12–32 years were divided into five groups according to the duration of the hirsutism (< 1, 1–2, 2–3, 3–5 and > 5 years). Peripheral plasma concentrations of LH and FSH, androstenedione, dehydroepiandrosterone sulphate, testosterone, 5α-dihydrotestosterone, 5α-androstane-3α, 17β-diol, 5α-androstane-3β, 17β-diol, cortisol, oestradiol-17β and oestrone were determined by radioimmunoassay. When the values obtained were compared with those from normal menstruating women, the results showed that in group I there was a significant increase only in the mean plasma 5α-androstane-3α, 17β-diol concentration. The mean concentration of this steroid was also raised in all other groups. In groups II and III mean basal levels of plasma dehydroepiandrosterone sulphate were also significantly increased and showed a marked increase after ACTH stimulation (1 mg tetracosactide acetate, i.m.) as did the concentrations of androstenedione and 17α-hydroxyprogesterone. Finally, in groups IV and V, a significant increase in mean plasma concentrations of LH, androstenedione, oestrone and testosterone was found in the basal condition. The clinical picture also became gradually more severe from group I to group V. These data suggest that hirsutism could be an evolving syndrome progressively involving peripheral androgen metabolism, the adrenal gland and finally the ovary possibly through alterations of hypothalamic-pituitary function.

PLASMA 20α-DIHYDROPROGESTERONE, PROGESTERONE AND 17-HYDROXYPROGESTERONE IN NORMAL HUMAN PREGNANCY

1977 ◽  
Vol 86 (3) ◽  
pp. 634-640 ◽  
Author(s):  
Emma Florensa ◽  
Robert Harrison ◽  
Michael Johnson ◽  
Ezat Youssefnejadian
Keyword(s):  
Plasma Concentration ◽  
Plasma Levels ◽  
First Trimester ◽  
Significant Drop ◽  
Human Pregnancy ◽  
Peripheral Plasma ◽  
Normal Human ◽  
First Trimester Of Pregnancy ◽  
The Mean ◽  
17 Hydroxyprogesterone

ABSTRACT The peripheral plasma levels of 20α-dihydroprogesterone (20α-DHP), progesterone (P) and 17-hydroxyprogesterone (17-OHP) were measured by radioimmuoassay techniques in 440 samples during normal human pregnancy between weeks 4 and 41. The levels of 20α-DHP in plasma from the 4th to the 6th week were between 6.0 and 6.6 ng/ml. From then until the 21st week the average plasma 20α-DHP concentrations remained at the same level between 4.0 and 6.3 ng/ml; they then rose significantly to and beyond term, levels reaching over 40 ng/ml. The range of mean plasma concentration of P during the first trimester of pregnancy fell to a nadir in the 9th week (170 ng/ml) then rose with increased gestation until the 39th week (190.4 ng/ml) followed by a slight and not significant drop. Single measurements of plasma 17-OHP from the 4th to the 6th week of pregnancy gave value between 2.8 and 3.6 ng/ml, but from the 7th week the mean plasma 17-OHP levels gradually declined, then from week 30 the 17-OHP concentration increased to reach a mean level of 7.63 ng/ml in the 41st week. The ratio P/20α-DHP increased from the 4th (3.5:1) to the 24th week (15.6:1) and then decreased from the 25th week (7.9:1) towards term (3.2:1).

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