scholarly journals Regulation of oxytocin receptor in the placentome capsule throughout pregnancy in the ewe: the possible role of oestradiol receptor, progesterone receptor and aromatase

1998 ◽  
Vol 158 (2) ◽  
pp. 173-181 ◽  
Author(s):  
ST Leung ◽  
TS Reynolds ◽  
DC Wathes
Keyword(s):  
Progesterone Receptor ◽  
Hormonal Regulation ◽  
Expression Patterns ◽  
In Situ Hybridisation ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Receptor Mrna ◽  
Oxytocin Receptors ◽  
Hybridisation Analysis

The hormonal regulation of uterine oxytocin receptors (OTR) during the establishment of pregnancy and at parturition has been studied extensively, but little information is available during mid-pregnancy. This study investigated the localisation of OTR mRNA in the ovine placentome throughout gestation and related this to expression patterns for the putative regulatory agents aromatase, oestradiol receptor, progesterone receptor and oxytocin. Placentomes were collected at regular intervals throughout pregnancy for in situ hybridisation analysis and immunocytochemistry (oestradiol receptor only). Results were quantified by optical density measurements of autoradiographs. Progesterone receptor mRNA was localised to the caruncular tissues on day 30 but became undetectable by day 34. Aromatase mRNA appeared in the fetal villi at days 34-40, with concentrations peaking at days 52-55 and again at days 132-137. Oestradiol receptor mRNA was localised to the caruncular tissues from days 13 to 30 and found in the maternal villi and placentome capsule from days 45 to 70. Oestradiol receptor protein was barely detectable in either tissue. OTR mRNA was localised to the placentome capsule at days 34-40, remaining high at day 45 and declining to basal levels by days 132-137. Oxytocin mRNA was not detected in the placentome. In conclusion: (1) progesterone acting via its receptor may suppress the expression of aromatase and OTR in early pregnancy; (2) the up-regulation of OTR expression in the capsule may not involve the oestradiol receptor; (3) there is a differential regulation between different regions of the uterus as the increase in the placentome capsule occurs at a time when concentrations in the rest of the endometrium and myometrium remain low; (4) oestradiol receptor expression in the placentome may be regulated at the translational level; and (5) there is no local production of oxytocin in the sheep placenta. The role of ORTs in the capsule during mid-pregnancy remains to be determined.

Androgen receptor expression in meningiomas

1995 ◽  
Vol 82 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Rona S. Carroll ◽  
Jianping Zhang ◽  
Kathleen Dashner ◽  
Madhabananda Sar ◽  
Elizabeth M. Wilson ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Northern Blot ◽  
Blot Analysis ◽  
Northern Blot Analysis ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Content Type ◽  
Messenger Ribonucleic Acid ◽  
Receptor Mrna

✓ The predominance of meningiomas in females, the accelerated growth of these tumors during the luteal phase of the menstrual cycle and during pregnancy, and the association between meningiomas and breast cancer have led to a number of studies examining the potential role of steroids in the growth of meningiomas. The possibility that androgens play a role in meningioma proliferation has been suggested by a small number of investigators. The aim of this study was to examine the expression of androgen receptor messenger ribonucleic acid (mRNA) and correlate it using immunochemistry with the nuclear localization of androgen receptor in a large number of meningiomas. Thirty-nine meningiomas were examined by Northern blot analysis for the presence of measurable amounts of androgen receptor mRNA and eight of these were analyzed by immunohistochemistry for receptor protein. Sixty-seven percent of the meningiomas expressed androgen receptor mRNA. There was a marked predominance of women among the patients whose tumors expressed androgen receptor; 69% were women and 31% were men. The immunohistochemical data correlated with Northern blot analysis of mRNA. The staining was predominantly nuclear, suggesting that the androgen receptor resides in a location that can activate gene expression.

scholarly journals Expression of oxytocin, oestrogen and progesterone receptors in uterine biopsy samples throughout the oestrous cycle and early pregnancy in cows

Reproduction ◽  
2001 ◽  
pp. 965-979 ◽  
Author(s):  
RS Robinson ◽  
GE Mann ◽  
GE Lamming ◽  
DC Wathes
Keyword(s):  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Oestrogen Receptor ◽  
Expression Patterns ◽  
Oestrous Cycle ◽  
Receptor Expression ◽  
Luminal Epithelium ◽  
Receptor Alpha ◽  
Oestrogen Receptor Alpha ◽  
Oxytocin Receptors

This study examined the expression patterns of oxytocin and steroid receptors in the bovine endometrium during the oestrous cycle and early pregnancy to elucidate their respective roles in the regulation of luteolysis and the maternal recognition of pregnancy. In Expt 1, uterine biopsies were collected from four cows throughout three oestrous cycles each, to provide daily samples. In Expt 2, uterine tissue was collected on days 12, 14, 16 and 18 of the oestrous cycle (n = 20) or early pregnancy (n = 16). Oxytocin receptor, oestrogen receptor alpha and progesterone receptor mRNAs were localized by in situ hybridization, and localization of oestrogen receptor and progesterone receptor was confirmed by immunocytochemistry. All three receptors showed time- and cell-specific expression patterns. Oestrogen receptor alpha increased in all regions at oestrus but high concentrations were also found in the luminal epithelium during the mid-luteal phase and in the deep glands throughout the oestrous cycle. Progesterone receptor expression was higher in the stroma than it was in the types of epithelial cell, and increased expression was observed at oestrus and during the early luteal phase. The cyclical upregulation of oxytocin receptors in the luminal epithelium on about day 16 was not related to preceding changes in the endometrial expression of either oestradiol alpha or progesterone receptors. During early pregnancy, oxytocin receptor expression was suppressed. Oestrogen receptor a concentrations increased in the non-pregnant cows and decreased in the pregnant cows between days 16 and 18, but these changes followed rather than preceded the upregulation of oxytocin receptors in the non-pregnant cows. It is concluded that the initial upregulation of oxytocin receptors in the luminal epithelium, which triggers luteolysis, is not associated directly with changes in expression of oestrogen receptor alpha.

Abstract 309: Sp-3 But not the NF-kB Transcription Factor Causes the Up-regulation of Angiotensin Type 1 Receptor Expression and Contributes to Hypertension in Aging FBN rats.

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Mohammad Saleem ◽  
Mohammad Asghar
Keyword(s):  
Blood Pressure ◽  
Transcription Factors ◽  
At1 Receptor ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Receptor Function ◽  
Receptor Mrna ◽  
Hk2 Cells ◽  
Angiotensin Type

We recently reported that age-associated oxidative stress is causal to higher renal angiotensin Type 1 (AT1) receptor function and hypertension in aged Fisher 344 X Brown Norway (FBN) rats. We became interested in examining the mechanism of higher AT1 receptor function in the aging kidneys. Adult (3-month) and aging (21 month) FBN rats were subjected to conscious blood pressure measurement by telemetry approach. The levels of AT1 receptor mRNA in the kidney cortex was measured by qRT-PCR while nuclear Sp-3 and NF-kB-p65 redox-sensitive transcription factors were determined by western blotting. We found that blood pressure was higher in aged than in adult rats (adult vs. old: 110±1 vs. 130±1 mmHg) which was associated with higher AT1 receptor mRNA levels (adult vs. old: 1.51±0.72 vs. 7.86±1.03 DU), and nuclear levels of both Sp-3 (adult vs. old: 0.56±.01 vs. 1.54±.02 DU) and NF-kB-p65 (adult vs. old: 0.9±.01 vs. 1.5±0.01 DU). To further delineate whether sp-3 or NF-kB-p65 or both transcription factors are responsible for the up-regulation of AT1 receptor, human kidney (HK2) cells were transfected with Sp-3 and NF-kB-p65 plasmids. We found that Sp-3 plasmid but not NF-κB-p65 plasmid transfection caused an increase in the levels of AT1 receptor protein in HK2 cells (control vs. transfected: 135±22 vs. 235±10 DU). Furthermore, Sp-3 siRNA treatment resulted in the reduction of Sp-3 (control vs. transfected: 136±10 vs. 93±21 DU) and AT1 receptor protein levels (control vs. transfected: 270±38 vs. 172±201 DU) in HK2 cells. Our results suggest that sp-3 but not the NF-κB-p65 is involved in the up-regulation of renal AT1 receptor that may be contributing to hypertension in aging FBN rats.

scholarly journals Caloric restriction reverses the deficits in leptin receptor protein and leptin signaling capacity associated with diet-induced obesity: role of leptin in the regulation of hypothalamic long-form leptin receptor expression

2004 ◽  
Vol 181 (2) ◽  
pp. 297-306 ◽  
Author(s):  
J Wilsey ◽  
PJ Scarpace
Keyword(s):  
Caloric Restriction ◽  
Leptin Receptor ◽  
Fold Increase ◽  
Receptor Expression ◽  
Male Rats ◽  
Receptor Protein ◽  
Leptin Signaling ◽  
Stat3 Phosphorylation ◽  
Long Form

The objectives of this study were to determine if reduced long-form leptin receptor (ObRb) expression in diet-induced obese (DIO) animals is associated with deficits in maximal leptin signaling and, secondly, to establish the effects of short-term caloric restriction (CR) on ObRb expression and function. Groups of DIO and life-long chow-fed (CHOW) F344xBN male rats, aged 6 months, were given an i.c.v. injection containing 2 micro g leptin or artificial cerebrospinal fluid (ACSF) vehicle. Leptin induced a >6-fold increase in STAT3 phosphorylation in CHOW rats, but less than 2-fold increase in DIO. Reduced maximal leptin-stimulated STAT3 phosphorylation in DIO rats was coupled with a decline in both ObRb expression and protein. At this point, subgroups of DIO and CHOW animals underwent CR for 30 days and were then tested for acute leptin responsiveness. CR resulted in a 45 and 85% increase respectively in leptin-stimulated STAT3 phosphorylation in CHOW and DIO animals. Similarly, CR increased ObRb expression and protein in both CHOW and DIO animals. To explore the role of leptin in regulating ObRb expression, we reversibly overexpressed leptin in the hypothalamus and found that ObRb mRNA inversely follows central leptin expression. By enhancing both ObRb expression and signaling capacity, CR may enhance leptin responsiveness in leptin-resistant DIO animals.

Parathyroid hormone/parathyroid hormone-related protein receptor expression in nude mice with a transplantable canine apocrine adenocarcinoma (CAC-8) and humoral hypercalcaemia of malignancy

1997 ◽  
Vol 153 (1) ◽  
pp. 123-129 ◽  
Author(s):  
A Gröne ◽  
L K McCauley ◽  
C C Capen ◽  
T J Rosol
Keyword(s):  
Parathyroid Hormone ◽  
Nude Mice ◽  
Elevated Serum ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Receptor Mrna ◽  
Tumour Bearing ◽  
Parathyroid Hormone Related Protein ◽  
Significant Difference ◽  
Pthrp Receptor

Abstract The effect of humoral hypercalcaemia of malignancy (HHM) on parathyroid hormone/parathyroid hormonerelated protein (PTH/PTHrP) receptor expression was investigated in nude mice with subcutaneous transplantation of an adenocarcinoma line (CAC-8) which produces PTHrP. Serum calcium and PTHrP concentrations were analysed by colorimetric assay and a two-site IRMA respectively. Mice were hypercalcaemic (3·3 ±0·1 mmol/l) compared with non-tumour-bearing control mice (2·1 ± 0·1 mmol/l) and had elevated serum PTHrP concentrations (30·4 ±3·4 pmol/l) compared with non-tumour-bearing control mice (0·7 ±0·1 pmol/l. Lumbar vertebrae were analysed by histomorphometry. Tumourbearing mice had a significant (P<0·01) increase in resorptive perimeter, increased numbers of osteoclasts/mm endosteum and increased endosteal bone-forming perimeter. Total RNA was isolated from calvarium, humerus and kidney and analysed for PTH/PTHrP receptor expression by Northern blot analysis. There was no significant difference between PTH/PTHrP receptor mRNA expression in the kidneys and humeri of tumourbearing mice compared with non-tumour control mice, but a significant increase in PTH/PTHrP receptor expression in calvaria. Kidneys and vertebral bodies were stained for PTH/PTHrP receptor protein by immunohistochemistry. Renal proximal tubules (especially the basolateral regions) and endosteal osteoblasts of control and tumourbearing mice stained positive for PTH/PTHrP receptor. These results demonstrated that HHM induced by increased serum PTHrP concentrations did not result in down-regulation of PTH/PTHrP receptor mRNA or protein expression in vivo. Journal of Endocrinology (1997) 153, 123–129

Intrauterine injection of ovine interferon-τ alters oestrogen receptor and oxytocin receptor expression in the endometrium of cyclic ewes

1995 ◽  
Vol 15 (2) ◽  
pp. 203-220 ◽  
Author(s):  
T E Spencer ◽  
N H Ing ◽  
T L Ott ◽  
J S Mayes ◽  
W C Becker ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Oestrogen Receptor ◽  
Plasma Concentrations ◽  
Oxytocin Receptor ◽  
Receptor Expression ◽  
Glandular Epithelium ◽  
Receptor Density ◽  
Receptor Mrna ◽  
Prostaglandin F

ABSTRACT This study determined the effects of intrauterine injections of recombinant ovine interferon-τ (roIFN-τ; 2 × 107 antiviral units/day) or control proteins (6 mg/day) from day 11 to day 14 post-oestrus (oestrus=day 0) on endometrial expression of receptors for oestrogen, progesterone and oxytocin in cyclic ewes. Plasma concentrations of progesterone were greater on day 15 in ewes receiving roIFN-τ compared with control proteins (P<0·02, treatment × day). Ewes injected with roIFN-τ had lower endometrial levels of oestrogen receptor mRNA (P<0·10) and protein (P<0·01) on day 15 compared with ewes receiving control proteins. In situ hybridization analysis indicated that oestrogen receptor mRNA was more abundant in the luminal and glandular epithelium of control ewes compared with roIFN-τ-treated ewes. Immunoreactive oestrogen receptor was also present in the luminal and glandular epithelium of control, but not roIFN-τ-treated ewes. Endometrial levels of progesterone receptor mRNA and protein were not different (P>0·10) between control and roIFN-τ-treated ewes. In situ hybridization analyses indicated that progesterone receptor mRNA abundance was low in endometrial epithelium and stroma of both control and roIFN-τ-injected ewes. Immunoreactive progesterone receptors were present in the endometrial stroma and epithelium of control ewes, but confined to the stroma of roIFN-τ-treated ewes. Oxytocin receptor density was lower (P<0·10) in the endometrium of ewes injected with roIFN-τ than control proteins; however, oxytocin receptor affinity was not affected (P>0·10) by treatment. Concentrations of 13,14-dihydro-15-keto-prostaglandin F2α (PGFM) were not increased by exogenous oxytocin administration in control and roIFN-τ-treated ewes on days 10 or 12 post-oestrus. However, on day 14, control ewes responded to oxytocin with increased plasma concentrations of PGFM, whereas ewes receiving roIFN-τ remained unresponsive to oxytocin. These results indicate that the antiluteolytic effects of IFN-τ are to prevent increases in endometrial oestrogen receptor mRNA and protein and oxytocin receptor density which abrogates uterine release of prostaglandin F2α during maternal recognition of pregnancy. IFN-τ may inhibit the synthesis of oestrogen receptor mRNA by a transcriptional or post-transcriptional regulatory mechanism to suppress oxytocin receptor formation during early pregnancy in ewes.

Age-dependent regulation of vasopressin V1a receptors in preglomerular vessels from the spontaneously hypertensive rat

2004 ◽  
Vol 286 (5) ◽  
pp. F997-F1003 ◽  
Author(s):  
Øyvind B. Vågnes ◽  
Frank H. Hansen ◽  
Rolf E. F. Christiansen ◽  
Camilla Gjerstad ◽  
Bjarne M. Iversen
Keyword(s):  
Spontaneously Hypertensive Rat ◽  
Mrna Level ◽  
Cytosolic Calcium ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto ◽  
Renal Vascular ◽  
Insight Into

Experiments were performed to get insight into the role of AVP receptor V1a regulation with age, i.e., during development and maintenance of high blood pressure. Previous studies showed an increased gene expression and renal vascular response to AVP in young spontaneously hypertensive rats (SHR). The age regulation of the V1a receptor was examined in preglomerular vessels from 5-, 10-, 20-, and 70-wk-old SHR using normotensive Wistar-Kyoto rats (WKY) as controls. Real-time PCR and ligand binding were used for analysis of receptor expression, and the change in cytosolic calcium concentration during stimulation of isolated preglomerular vessels with AVP was studied. Studies showed an increase of the V1a receptor protein and mRNA from 5-and 10-wk-old SHR compared with vessels from 20- and 70-wk-old SHR. In 5-wk-old SHR receptor density was 84 ± 13 fmol/mg protein, and 38 ± 11 fmol/mg protein in 70-wk-old SHR ( P < 0.05). mRNA in the 5- and 70-wk-old SHR was 15,854 ± 629 and 3,181 ± 224 V1a mRNA/108 18S ribosomal RNA, respectively ( P < 0.001). Values from WKY at all ages were similar to 20- and 70-wk-old SHR. During stimulation with AVP, the change in cytosolic calcium in vessels from 5-wk-old SHR increased 234 ± 59 nM, whereas the increase was 89 ± 9 nM in 70-wk-old SHR ( P = 0.03). These results indicate that the V1a receptor is increased at protein and mRNA level during development of hypertension in SHR but is normalized when hypertension is established.

scholarly journals Opioid Receptor Protein Expression During Development of the Rat Brainstem

2021 ◽  
Author(s):  
◽  
Bronwyn Maree Kivell
Keyword(s):  
Confocal Microscopy ◽  
Opioid Receptor ◽  
Glial Cells ◽  
Opioid Receptors ◽  
Cultured Cells ◽  
Expression Patterns ◽  
Receptor Expression ◽  
Developmental Expression ◽  
Receptor Protein ◽  
Western Blots

<p>Few satisfactory protocols exist for primary culture of postnatal brainstem neurons, and commonly used procedures often give poor survival rates in older foetal (>E16) and early postnatal brainstem cultures. The present study describes the first reliable method for establishing stable in vitro cultures of foetal and postnatal brainstem neurons up to six days postnatal age in a defined, serum-free culture medium. This novel culture method was used to study opioid receptor expression and distribution in developing brainstem cells. Opioids play an important role in brainstem functions, being involved in respiratory and cardiovascular modulation and pain control (Olsen et al., 1995; Olson et al., 1997; Vaccarino et al., 1999; Vaccarino and Kastin, 2001). These brainstem functions are particularly important for survival at birth, and opioid receptor distribution patterns and sensitivities to opioid ligands change during development. Using cultured cells and frozen sections of brainstem tissue, mu (MOR) and delta (DOR) opioid receptor localisation in neuronal and glial cells at different stages of foetal and postnatal development in the rat were examined by immunocytochemistry and confocal microscopy. Bipolar and multipolar neurons showed similar immunoreactivities; whereas, glial cells were more lightly stained than neurons. Developmentally advanced stages were more intensely stained for MOR (P<0.006, Mann-Whitney test); whereas, DOR immunoreactivity did not change during development. These developmental expression patterns observed in culture for MOR were similar to those obtained from Western blots of electrophoreses brainstem lysates. DOR, however, decreased in expression in brainstem lysates with increased developmental age, even though there was no difference in DOR expression in cultured cells. MOR and DOR were colocalised in specific brainstem regions and in the cerebellum of foetal and postnatal animals, although the distribution of both opioid receptors in the foetal brain was more diffuse than in the older animals. The intracellular distributions of MOR and DOR were investigated by confocal microscopy. In addition to plasma membrane staining, a population of internalised cytoplasmic receptors was present in neurons. MOR was down-regulated after exposure of either cultured brainstem cells or transfected or non-transfected SH-SY5Y neuroblastoma cells to the MOR agonist DAMGO. From the above investigation, it was concluded that opioid receptors are developmentally regulated during maturation of the brainstem of the rat, and that primary cell culture, immunocytochemistry, and immunoblotting of cell lysates are suitable techniques for investigating opioid systems in the foetal, postnatal, and adult rat.</p>

Biological Role of Pituitary Estrogen Receptors ERα and ERβ on Progesterone Receptor Expression and Action and on Gonadotropin and Prolactin Secretion in the Rat

2004 ◽  
Vol 79 (5) ◽  
pp. 247-258 ◽  
Author(s):  
José E. Sánchez-Criado ◽  
Juana Martín de las Mulas ◽  
Carmina Bellido ◽  
Manuel Tena-Sempere ◽  
Rafaela Aguilar ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Estrogen Receptors ◽  
Prolactin Secretion ◽  
Receptor Expression ◽  
Biological Role ◽  
Progesterone Receptor Expression

scholarly journals Crucial Role of DNA Methylation in Determination of Clonally Distributed Killer Cell Ig-like Receptor Expression Patterns in NK Cells

2002 ◽  
Vol 169 (8) ◽  
pp. 4253-4261 ◽  
Author(s):  
Simeon Santourlidis ◽  
Hans-Ingo Trompeter ◽  
Sandra Weinhold ◽  
Britta Eisermann ◽  
Klaus L. Meyer ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Nk Cells ◽  
Crucial Role ◽  
Expression Patterns ◽  
Killer Cell ◽  
Receptor Expression
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