scholarly journals Vegetative and micropropagation of leucaena

2019 ◽  
Vol 7 (2) ◽  
pp. 87-95
Author(s):  
Travis Idol ◽  
Adel Youkhana ◽  
Renier Paul Santiago
Keyword(s):  
Tissue Culture ◽  
User Requirements ◽  
Stock Plant ◽  
Rooted Cuttings ◽  
Keynote Paper ◽  
Tap Root ◽  
Skill Requirement ◽  
Air Layers ◽  
Propagation Media ◽  
Sterile Hybrids

Keynote paper presented at the International Leucaena Conference, 1‒3 November 2018, Brisbane, Queensland, Australia.To effectively utilize sterile hybrids of leucaena, efficient protocols for vegetative propagation are needed that meet different user requirements and capabilities. We developed and compared methods for propagating several sterile hybrids of leucaena and compared them with each other and with propagation via seeds for variety K636. Methods included air-layers, rooted cuttings, grafting and tissue culture (micropropagation). All methods required 14‒20 weeks from generation of new shoots on the stock plant to production of rooted plantlets ready to outplant as compared with 6‒8 weeks for seedlings of K636. Successful rooting was highest for air-layers and rooted cuttings. Grafting had lower success owing to a higher skill requirement for the propagator. Tissue culture showed promise, but use of field-grown material was limited by microbial contamination of propagation media. Rooted cuttings are the best option presently for operational-scale propagation, but the method requires a mist system or a carefully controlled non-mist environment. If an effective method can be developed, grafting of young shoots onto a seedling rootstock is an alternative that retains the advantages of a seedling tap root and requires fewer resources than rooted cuttings or tissue culture.

Tumor Incidence and Shoot Morphology Differences in Rooted Cuttings from Rhododendron With and Without “Tissue Proliferation”

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478d-478
Author(s):  
Mark H. Brand ◽  
Richard Kiyomoto
Keyword(s):  
Tissue Culture ◽  
Leaf Size ◽  
Plant Age ◽  
Tumor Incidence ◽  
Stock Plant ◽  
Rooted Cuttings ◽  
Shoot Morphology ◽  
Size And Shape ◽  
Tissue Proliferation ◽  
Leaf Size And Shape

Tissue proliferation (TP) occurs in Rhododendron and is characterized by basal tumors that often develop numerous dwarf shoots. Growers need to know if the TP condition will persist in plants grown from normal-appearing cuttings collected from plants with TP tumors. Cuttings of seven cultivars were collected from plants with TP [TP(+)] and without TP [TP(-)] and rooted. Plants were grown in containers outdoors for a minimum of 2 years. For all cultivars, plants grown from TP(+) cuttings had more leaves per growth flush than plants grown from TP(-) cuttings. Shoots of TP(+) plants were either similar in length to shoots of TP(-) plants, or were shorter, as was the case for `Boule de Neige', `Catawbiense Album', and `Montego'. `Montego' and `Scintillation' TP(+) leaves were shorter, narrower, had smaller leaf areas and larger length to width rations than leaves from TP(-) shoots. No difference in leaf size and shape were detected for other cultivars. TP tumors were not observed on any plants. Another study examined the effect of stock plant age from tissue culture on development of TP symptoms in rooted cuttings. `Montego' plants grown from cuttings collected from microplants, 3-year-old TP(+) plants, 6-year-old TP(+) plants and TP(-) plants were compared. Plants grown from 6-year-old TP(+) and TP(-) cuttings did not develop tumors. 83% of plants from microplant cuttings and 74% of plants from 3-year-old TP(+) cuttings formed tumors. Plants from microplant cuttings were more likely to develop large tumors that surround half or more of the stem than plants from 3-year-old TP(+) cuttings.

scholarly journals Propagation of Ulmus parvifolia ‘Emerald Prairie’ by Stem Cuttings

2004 ◽  
Vol 22 (2) ◽  
pp. 55-57
Author(s):  
Jason J. Griffin ◽  
Kenneth R. Schroeder
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Stem Cuttings ◽  
Root Number ◽  
Stock Plant ◽  
Rooted Cuttings ◽  
Rooted Cutting ◽  
Ulmus Parvifolia ◽  
Survival And Growth ◽  
Terminal Shoot

Abstract Stem cuttings of Ulmus parvifolia Jacq. ‘Emerald Prairie’ (‘Emerald Prairie’ lacebark elm), consisting of 7.5 to 10 cm (3 to 4 in) terminal shoot portions, were collected four times throughout the year from mature stock plants and treated with the potassium (K) salt of indole-3-butyric acid (K-IBA) ranging from 0 to 20,000 ppm (2.0%). Rooting percentages were affected greatly by K-IBA treatment and stock plant growth stage. Little to no rooting was observed without K-IBA application, whereas overall rooting for treated softwood, early semi-hardwood, late semi-hardwood, and hardwood, cuttings was 92, 86, 87, and 8%, respectively. Mean root number varied by growth stage and K-IBA concentration. Higher concentrations of K-IBA increased the number of roots on rooted cuttings except for hardwood cuttings. In general, rooting percentage and mean root number were highest at the softwood stage with 15,000 or 20,000 ppm (1.5 or 2.0%) K-IBA, resulting in 97% rooting and 15 or 22 roots per rooted cutting, respectively. However, leaf abscission was high on softwood cuttings. Semi-hardwood cuttings achieved similar rooting percentages and may exhibit better survival and growth due to greater leaf retention.

Plant genotype, juvenility and mechanisms of inhibition of rooting Persoonia virgata R. Br. Cuttings

1999 ◽  
Vol 39 (8) ◽  
pp. 1029 ◽  
Author(s):  
L. M. Bauer ◽  
M. E. Johnston ◽  
R. R. Williams
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Root Formation ◽  
Limiting Factor ◽  
Genotypic Differences ◽  
Stock Plant ◽  
Plant Genotype ◽  
Rooted Cuttings ◽  
Selection Of ◽  
Better Than

Summary. Persoonia virgata is an endemic shrub of Australia that is being bush-harvested for its foliage and sold on the domestic and export flower markets. The limiting factor to the cultivation of many desirable Persoonia species has been the extreme difficulty in developing a reliable propagation system. This study investigated the effects of cutting type, maturity of the stock plant, and genotype, on the rooting of P. virgata cuttings. It was found that juvenile cuttings rooted significantly better than mature cuttings. Some juvenile seedling genotypes yielded no rooted cuttings, whereas others produced up to 90% rooting. No anatomical barrier to root formation was discovered. High performance liquid chromatography showed differences in the abundance of unidentified compounds in cuttings, related to season and to genotypic differences in rooting abilities. A possible seasonal rooting inhibitor was present in some genotypes. The potential for selection of genotypes with improved rooting ability has been demonstrated.

Propagation of Abiesfraseri by cuttings: orthotropic shoot production from hedged stock plants

1986 ◽  
Vol 16 (2) ◽  
pp. 226-231 ◽  
Author(s):  
Farrell C. Wise ◽  
Frank A. Blazich ◽  
L. Eric Hinesley
Keyword(s):  
Growing Season ◽  
Shoot Formation ◽  
Low Fertility ◽  
Separate Study ◽  
Stock Plant ◽  
Rooted Cuttings ◽  
Fraser Fir ◽  
Chemical Treatments ◽  
Christmas Trees ◽  
Shoot Production

Fraser fir (Abiesfraseri (Pursh) Poir.) stock plants grown as transplants (4 years old) or sheared Christmas trees (14 years old) were subjected to independent combinations of hedging and chemical treatments to induce orthotropic shoot production. Decapitation treatments that removed the most top growth produced the greatest number of orthotropic shoots in each age-class after one growing season. Sprays of dikegulac at 1000 and 3000 ppm did not stimulate orthotropic shoot formation. Orthotropic shoots induced on maturing Christmas trees rooted in lower percentages than plagiotropic cuttings from similar nondecapitated trees. Since rooting and survival of the severed shoots were largely or totally unaffected by hedging treatments, decapitation treatments that produced the most shoots the 1st year continued to exhibit the most rooted cuttings at the end of the following season. At that time, superior treatments on 4- and 14-year-old plants had produced less than three and five rooted orthotropic shoots per stock plant, respectively. The stock plants received no additional hedging and were evaluated for shoot production after a second season. No treatment differences occurred within the older plants, but superior treatments from the 1st year tended to be retained within the transplants. In a separate study, orthotropic shoot production by severe decapitation was enhanced by maintaining low fertility.

scholarly journals Novel Laboratory Exercises in Plant Tissue Culture: In Vitro Asymbiotic Germination of Orchid Seeds

1994 ◽  
Vol 4 (3) ◽  
pp. 315-317 ◽  
Author(s):  
Kenneth W. Mudge ◽  
Chin-Chang Chu
Keyword(s):  
Tissue Culture ◽  
Plant Material ◽  
Stock Plant ◽  
Asymbiotic Seed Germination ◽  
Laboratory Exercise ◽  
Flower Pollination ◽  
Orchid Seed ◽  
Plant Flower ◽  
Single Laboratory

In vitro asymbiotic seed germination, subculture, and outplanting of orchids is presented as a laboratory exercise suitable for students of plant propagation or tissue culture. Dendrobium antennatum (Lindley), Phalaenopsis (Blume) white hybrid, or both, are used in this exercise because they flower predictably in the greenhouse, are reliable for seed production, and germinate and grow rapidly in vitro. The exercises can be used to instruct students in the skills involved in orchid seed sterilization, sowing, and culture, as well as instruct students in the unique features of orchid reproductive biology and symbiosis. A schedule is suggested for stock plant flower pollination, capsule harvest, seed sowing, and seedling subculture so that the necessary plant material is available for students to sow, subculture, and outplant seedlings during a single laboratory session.

scholarly journals The Effect of Olive Tree Stock Plant Nutritional Status on Propagation Rates

HortScience ◽  
2012 ◽  
Vol 47 (2) ◽  
pp. 307-310 ◽  
Author(s):  
Arnon Dag ◽  
Ran Erel ◽  
Alon Ben-Gal ◽  
Isaac Zipori ◽  
Uri Yermiyahu
Keyword(s):  
Nutritional Status ◽  
Olive Tree ◽  
Significant Negative Correlation ◽  
Propagation Rate ◽  
N Fertilization ◽  
Root Weight ◽  
Stock Plant ◽  
High Concentration ◽  
Rooted Cuttings ◽  
K Concentration

The global production of olives (Olea europaea L.) has increased rapidly over the last decade as a result of the expansion of orchards with high tree densities. Most olives are propagated from rooted cuttings. The present study evaluated the propagation rate of rooted cuttings as a function of the nutritional status of the stock trees. Rooting ability was evaluated for cuttings taken from container-grown stock plants exposed to eight concentrations of nitrogen (N) (ranging from 0.4 to 14.1 mm), seven concentrations of phosphorus (P) (ranging from 0.01 to 0.62 mm), and five concentrations of potassium (K) (ranging from 0.25 to 5.33 mm). Increases in N level negatively affected rooting rate and cutting survival. Propagation success was increased threefold as N in irrigation water was reduced from the highest to the lowest treatments. Enhanced root development under low N concentrations resulted in higher root weight compared with the high N concentrations. The high concentration of N fertilization negatively affected the propagation rate but was not reflected in N concentration of diagnostic leaves. There was, however, a significant negative correlation between N in twigs and propagation rate. Regarding response to K concentration, no effect was found on rooting rate or cutting survival. Except for reduced rooting at the lowest concentration, P had a negligible effect on rooting rate. The experimental results indicate the need to avoid overfertilization of olive stock trees with N to promote successful propagation.

scholarly journals Effect of Different Concentrations of Growth Regulators on Gardenia jasminoides cv. Veitchii Micropropagation by Tissue Culture Technique

2003 ◽  
Vol 8 (1) ◽  
pp. 35 ◽  
Author(s):  
G. R. Abdullah ◽  
A.A. Al-Khateeb ◽  
M. Serage
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Culture Technique ◽  
Nutrient Media ◽  
Gardenia Jasminoides ◽  
Secondary Culture ◽  
Set Up ◽  
One Year ◽  
Sprout Growth ◽  
Propagation Media

Micropropagation techniques were set up for Gardenia jasminoides c.v. veitchi. Many plantlets were obtained by culturing shoot cuttings in MS nutrient media, 30 g/L Sucrose, 7 g/L Agar Agar, and different concentrations of BAP and IAA. The best concentration was 1mg /L BAP with 0.5 mg/L IAA. This concentration gave the best sprout growth suitable for rooting in primary and secondary culture by reculturing the stuck cutting every 6 weeks and for many times. We also obtained a high rooting percentage up to 98 % of natural rooting in rooting media different from propagation media by reducing mineral salt concentration to half, Sucrose to 20gm/L, and 2gm/L active charcoal, and 1mg/L IAA. Plantlets were transferred to greenhous and subjected for hardening. This technique gave 22 plantlets from one cutting in one year.

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