A Study on Morphological Growth and Development of Swiss Albino Mice, Wistar Rat and Dunkin Hartley Guinea Pigs

Background: Baseline information on the morphological development of laboratory animals is very scanty. Hence the present study was undertaken to understand the morphological development of experimental animals. Methods: An experiment was conducted at Biogen animal facility, Bangalore in the year 2018 to study the morphological changes with regard to growth and developmental parameters in Swiss albino mice and Wistar rats and body weight in guinea pigs. Ten Swiss albino mice and Wistar rats in advanced pregnancy of similar age groups and comparable litter sizes in the previous kindlings were selected for the experiment, where as five guinea pigs males and females each were selected with same age group. During the study period, litter weight and size at birth and at weaning, time of initiation and completion (full growth of hair) of hair growth, time of opening of eyes and ears recorded in Swiss albino mice and Wistar rats whereas body weight at birth, 3rd day, 6th day, 9th day, 12th day and 15th were recorded in Dunkin Hartley guinea pigs. Result: The results of Swiss albino mice indicated that the average litter weight (grams) and litter size at birth ranged from 1.39 and 7.50 respectively. The average time taken from initiation and full growth of hair covering on body was 7.50 to 15.30 days. Eyes and ears opened at 7.90 and 8.20 days respectively. Average weight of male and female recorded at weaning (25 days) 19.38 and 15.12 respectively and the litter size at the weaning was 9.70 whereas, livability percent was recorded 87.81 at the end of the trial. The results of Wistar rats indicated that the average litter weight (grams) and litter size at birth ranged from 5.07 and 11 respectively. The average time taken from initiation to full growth of hair covering on body was 9 to 16.20 days. Eyes and ears opened at 12.20 days, average weight (grams) of male and female recorded at weaning (25 days) 78.03 and 63.09 respectively. The litter size at the weaning was 9.70. The livability percent was recorded 88.02 at the end of the trial. The results of Dunkin Hartley guinea pigs indicated that the average body weight (grams) of female at birth, 3rd day, 6th day, 9th day, 12th day, 15th (Weaning period) ranged from 94.88, 109.34, 123.94, 139.74, 152.14 and 166.66, respectively. On the other hand, average body weight (grams) of male at birth, 3rd day, 6th day, 9th day, 12th day, 15th (Weaning period) ranged from 145.38, 155.42, 170.50, 185.54, 200.64 and 215.7, respectively.

Predictors of Underweight among Children Under-five in Ethiopia

9013 under-five children were included for study. Descriptive Statistics and POM were employed identify socio-economic, demographic, and proximate factors of underweight to children among regions of Ethiopia. SAS version 9.4 was used. About 25.3% of children in Ethiopia were underweighted. POM showed that sex, residence, size at birth, age group, incidence of diarrhea, educational level, breastfeeding status, mothers’ employment status, and birth order were found to be significant factors of underweight. Improvement of education for caring child in appropriate age needed. Personal hygiene be improved to prevent exposures to diarrhea and environmental sanitation.

Genetic and non-genetic effects associated with ewe productivity in Harnali sheep

Summary The objective of the current study was to estimate the genetic parameters for ewe productivity traits of Harnali sheep by examining non-genetic effects. The data records of 440 animals born to 85 sires and 259 dams were collected with respect to various traits such as litter size at birth (LSB), litter weight at birth (LWB), litter size at weaning (LSW), litter weight at weaning (LWW) and age at first lambing (AFL) for the period of 2001 to 2020. Genetic parameters were estimated by fitting a series of animal models using an average information restricted maximum likelihood (REML) algorithm in WOMBAT software. Least-squares analysis revealed significant (P < 0.05) influences of period of lambing, age and weight of ewe at lambing on the studied traits. These results indicated that heavier ewes had significantly higher (P < 0.05) values of litter weight traits than their counterparts. On the basis of likelihood ratio test, the estimates of direct heritability under best model for AFL, LSB, LWB, LSW and LWW were 0.06, 0.18, 0.09, 0.07 and 0.16, respectively. Maternal permanent environment effect made a significant contribution to the LSB trait (0.20). The genetic correlation between litter size and LWW was negative, while the remaining correlations were positive. The present results suggest that selection based on ewe productivity traits will result in low genetic progress and therefore the management role is more important for better gains.

Second-Hand Smoke Exposure inside the House and Adverse Birth Outcomes in Indonesia: Evidence from Demographic and Health Survey 2017

Objectives: Second-hand Smoke (SHS) during pregnancy among non-smoking women associates with mortality and morbidity risks in their infants. However, little is known about the SHS inside the house and the adverse birth outcomes. This study aims to assess the prevalence, level, and frequency of SHS exposure inside the house and investigate their associations with birth outcomes. Methods: We use the Indonesian Demographic and Health Survey (IDHS) 2017, a large-scale nationally representative survey. Women aged 15 to 49 years who had given birth in the last five years before the study and their husbands were interviewed (n=19,935). Three dependent variables included Low Birth Weight (LBW), size at birth, and birth weight. Results: Seventy-eight percent of mothers exposed to SHS inside home, of whom 7.2% had LBW children. Compared to non-SHS exposure mothers, those exposed to SHS were younger, had first birth before 20 years old, married, lower educated, non-worker, lived in rural, grand multipara, had pollution from cooking fuel, cook in a separate building, had higher risk of delivering lower birth weight (aOR=1.16, 95% CI: 1.02, 1.33), and smaller baby (aOR=1.51, 95%CI: 1.35, 1.69), even after the controlling for the covariates. We identified the inverted U-shaped association for SHS exposure frequency. Similar risk was also observed among mothers exposed with SHS on a daily basis compared to those who are not exposed. Conclusion: Exposure to SHS inside home was significantly associated with LBW and size at birth. Given the high smoking prevalence, relevant policy and health promotion are needed.

Incidence and predictors of under-five mortality in East Africa using multilevel Weibull regression modeling

Background In 2017, an estimated 5.3 million under-five children died annually in Sub-Saharan African countries, more than half of those deaths occurred in East Africa. Though East African countries share the huge burden of global under-five mortality, there is limited evidence on the incidence and predictors of under-five mortality. Therefore, this study investigated the incidence and predictors of under-five mortality in East Africa. Methods A community-based cross-sectional study was done based on the Demographic and Health Survey (DHS) data of 12 East African countries conducted from 2008 to 2019. A total weighted sample of 138,803 live births within 5 years preceding the survey were included for analysis. The Kaplan-Meier curve and Log-rank test were done to assess the children’s survival experience across variable categories. The Global Schoenfeld residual test was employed for checking Proportional Hazard (PH) assumptions and it was violated (p-value< 0.05). Considering the hierarchical nature of DHS data, multilevel parametric survival models were fitted. Model comparison was made by AIC, deviance, and shape of the hazard function. Variables with a p-value of less than 0.2 in the bi-variable analysis were considered for the multivariable analysis. In the multilevel Weibull regression analysis, the Adjusted Hazard Ratio (AHR) with 95% Confidence Interval (CI) was reported to declare the significant predictors of under-five mortality. Results Under-five mortality rate in East Africa was 51.318 (95% CI: 51.311, 51.323) per 1000 live births. Babies born to mothers attained secondary education and above (AHR = 0.83, 95% CI: 0.75, 0.91), being 2nd - 4th birth order (AHR = 0.62, 95% CI: 0.56, 0.67), ≥ 5th birth order (AHR = 0.68, 95% CI: 0.61, 0.76), health facility delivery (AHR = 0.87, 95% CI: 0.82, 0.93), 1–3 ANC visit (AHR = 0.61, 95% CI: 0.54, 0.68), births interval of 24–48 months (AHR = 0.53, 95% CI: 0.50, 0.57), wanted pregnancy (AHR = 0.72, 95% CI: 0.68, 0.76), middle wealth status (AHR = 0.90, 95% CI: 0.83, 0.97), and richest wealth status (AHR = 0.81, 95% CI:0.73, 0.90) were significantly associated with lower hazards of under-five mortality. Whereas, advanced maternal age (≥35 years) (AHR = 1.13, 95% CI: 1.04, 1.24),, babies born to household who did not have media exposure (AHR = 1.13, 95% CI: 1.07, 1.20), twin births (AHR = 3.81, 95% CI: 3.52, 4.12), being male child (AHR = 1.27, 95%CI: 1.21, 1.33), small birth size at birth (AHR = 1.73, 95% CI: 1.63, 1.84), and large size at birth (AHR = 1.11, 95% CI: 1.04, 1.11) were significantly associated with higher hazards of under-five mortality. Conclusion Under-five mortality is a major public health concern in East African countries. Health facility delivery, ANC visit, higher wealth status, adequate birth spacing, wanted pregnancy, and maternal education were significantly correlated with a lower risk of under-5 mortality. Whereas, higher birth order, small or large size at birth, male birth, twin birth, advanced maternal age and mothers who didn’t have media exposure were significantly correlated with a higher risk of under-five mortality. This study highlights that public health programs should enhance health facility delivery, ANC visit, media exposure, maternal education, and adequate birth spacing to decrease the incidence of under-five mortality in East Africa.

Determinant factors of under-five mortality in Southern Nations, Nationalities and People’s region (SNNPR), Ethiopia

Background Child mortality is a key indicator of the performance of the health system of a nation. Impressive progress in the reduction of under-five mortality has been made in Ethiopia. However, still there are some regions where the under-five mortality rates are high. Southern Nations, Nationalities, and Peoples’ Region (SNNPR) is among those regions in Ethiopia with high under-five mortality rates. This study aimed to identify the determinant factors of under-five mortality in SNNPR. Methods Data used for the study were drawn from the 2016 EDHS. A total of 1277 under-five children were included in the study. A multivariable logistic regression model was fitted to identify determinant factors associated with under-five mortality. Results Children with second or third birth order (OR = 1.316, 95% CI: (1.097, 2.343)), fourth or fifth birth order (OR = 1.934, 95% CI: (1.678, 3.822)), sixth or above birth order (OR = 3.980, 95% CI: (2.352, 6.734)) were significantly associated with increased risk of under-five mortality as compared to those with first birth order. Increased risk of under-five mortality was also significantly associated with a family size of five or more (OR = 3.397, 95% CI: (1.702, 6.782)) as compared to the family size of less than five; smaller size at birth (OR = 1.714, 95% CI: (1.120, 2.623)) as compared to larger size at birth; multiple births (OR = 1.472, 95% CI: (1.289, 2.746)) as compared to singletons. On the other hand, female children (OR = 0.552, 95% CI: (0.327, 0.932)), children born at health institutions (OR = 0.449, 95% CI: (0.228, 0.681)) and children who were breastfed (OR = 0.657, 95% CI: (0.393, 0.864)) were significantly associated with decreased risk of under-five mortality as compared to male children, those born at home and those who were not breastfed respectively. Conclusions Sex of a child, birth order, size of a child at birth, place of delivery, birth type, breastfeeding status, and family size were significant factors associated with under-five mortality in SNNPR, Ethiopia. Thus, planning and implementing relevant strategies that focus on those identified determinant factors of under-five mortality is required for the improvement of child survival in SNNPR, Ethiopia.

Size at birth, lifecourse factors, and cognitive function in late life: findings from the MYsore study of Natal effects on Ageing and Health (MYNAH) cohort in South India

ABSTRACT Objective: To examine if smaller size at birth, an indicator of growth restriction in utero, is associated with lower cognition in late life, and whether this may be mediated by impaired early life brain development and/or adverse cardiometabolic programming. Design: Longitudinal follow-up of a birth cohort. Setting: CSI Holdsworth Memorial Hospital (HMH), Mysore South India. Participants: 721 men and women (55–80 years) whose size at birth was recorded at HMH. Approximately 20 years earlier, a subset (n = 522) of them had assessments for cardiometabolic disorders in mid-life. Measurements: Standardized measurement of cognitive function, depression, sociodemographic, and lifestyle factors; blood tests and assessments for cardiometabolic disorders Results: Participants who were heavier at birth had higher composite cognitive scores (0.12 SD per SD birth weight [95% CI 0.05, 0.19] p = 0.001) in late life. Other lifecourse factors independently positively related to cognition were maternal educational level and participants’ own educational level, adult leg length, body mass index, and socioeconomic position, and negatively were diabetes in mid-life and current depression and stroke. The association of birth weight with cognition was independent cardiometabolic risk factors and was attenuated after adjustment for all lifecourse factors (0.08 SD per SD birth weight [95% CI −0.01, 0.18] p = 0.07). Conclusions: The findings are consistent with positive effects of early life environmental factors (better fetal growth, education, and childhood socioeconomic status) on brain development resulting in greater long-term cognitive function. The results do not support a pathway linking poorer fetal development with reduced late life cognitive function through cardiometabolic programming.

Under-5 Mortality and Its Associated Factors in Northern Nigeria: Evidence from 22,455 Singleton Live Births (2013–2018)

The northern geopolitical zones (NGZs) continue to report the highest under-5 mortality rates (U5MRs) among Nigeria’s six geopolitical zones. This study was designed to identify factors related to under-5 mortality (U5M) in the NGZs. The NGZ populations extracted from the 2018 Nigeria Demographic and Health Survey were explored to assess the factors associated with U5M using logistic regression, generalised linear latent, and mixed models. Between 2013 and 2018, the northwest geopolitical zone reported the highest U5MR (179 deaths per 1000 live births; 95% confidence interval [CI]: 163–194). The adjusted model showed that geopolitical zone, poor household, paternal occupation, perceived children’s body size at birth, caesarean delivery, and mothers and fathers’ education were highly associated with increased odds of U5M. Other significant factors that influenced U5M included children of fourth or higher birth order with shorter interval ≤ 2 years (adjusted odds ratio [aOR] = 1.68; CI: 1.42–1.90) and mothers who did not use contraceptives (aOR = 1.41, CI: 1.13–1.70). Interventions are needed and should primarily spotlight children residing in low-socioeconomic households. Educating mothers on the benefits of contraceptive use, child spacing, timely and safe caesarean delivery and adequate care for small-sized babies may also reduce U5M in Nigeria, particularly in the NGZs.