scholarly journals Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh

2008 ◽  
Vol 52 (No. 4) ◽  
pp. 83-87 ◽  
Author(s):  
A. Matějíček ◽  
J. Matějíčková ◽  
E. Němcová ◽  
O.M. Jandurová ◽  
M. Štípková ◽  
...  

The objective of this study was to estimate the joint effects of <i>CSN3</i> and <i>LGB</i> genotypes on breeding values of milk production parameters. <i>CSN3</i> (kappa-casein) and <i>LGB</i> (beta-lactoglobulin) genotypes of 120 Czech Fleckvieh sires were detected using the PCR-RFLP method. Breeding values of sires were obtained from the Official Database of Progeny Testing. Ten genotype combinations were detected. Genotypes <i>ABAB</i> (25.0%), <i>ABAA</i> (13.3%) and <i>ABBB</i> (13.3%) were the most frequent. Significant effects of genotype combinations on breeding values for fat and protein content were found. The highest breeding values for milk (+621 kg) and protein (+15.8 kg) yields were associated with genotype combination <i>ABAA</i>, while the highest breeding values for content parameters (+0.15% for protein content and +0.55% for fat content) were associated with genotype combination <i>BBAB</i>.

2011 ◽  
Vol 51 (No. 6) ◽  
pp. 241-247 ◽  
Author(s):  
J. Kučerová ◽  
A. Matějíček ◽  
Jandurová OM ◽  
P. Sørensen ◽  
E. Němcová ◽  
...  

Genotypes of milk protein genes, alpha<sub>S1</sub>-casein (CSN1S1), beta-casein (CSN2), kappa-casein (CSN3) and beta-lactoglobulin (LGB), were detected in 440 individuals of Czech Fleckvieh breed using a PCR-RFLP method. Breeding values of genotyped animals were obtained from the Official Database of Progeny Testing. A granddaughter design including 5 grandsires, 33 sons and 402 granddaughters was used for evaluation. Statistical analysis was carried out in SAS program using a restricted maximum likelihood method (REML). The aim of this study was to determine allele and genotype frequencies of observed genes and to find the relation between genotypes of genes and milk production parameters expressed by the breeding values of animals. The positive findings of frequencies of CSN3 allele B (0.38) and genotype BB (13%) were detected in the observed population. Significant differences were found between genotypes of loci CSN1S1, CSN2 and CSN3 and breeding values for milk production parameters. Genotypes of LGB locus were not significantly related to any of the milk production parameters. Significant effects were found between CSN1S1 locus and average breeding value for milk yield, CSN2 locus and breeding values for all parameters except for protein content and CSN3 locus and breeding values for protein content and protein yield. These findings contribute to a better understanding of the genetic constitution of Czech Fleckvieh breed and help with the application of the information to breeding. &nbsp; &nbsp;


2017 ◽  
Vol 53 ◽  
pp. 215-221
Author(s):  
O. A. Epishko ◽  
V. V. Peshko ◽  
N. N. Peshko

In cows of the Belarusian black-motley breed using PCR-RFLP method set gene polymorphism of beta-lactoglobulin. Identified genotypes LGBAA, LGBAВ and LGBВВ. Calculated frequency of alleles and genotypes in the gene beta-lactoglobulin. Studied milk productivity (yield of milk, fat and protein content of the milk, the amount of milk fat and milk protein) in animals with different genotypes of beta-lactoglobulin. Established the superiority of the cows with genotype LGBВВ the main indicators of milk production.


Author(s):  
Renáta Toušová ◽  
Jaromír Ducháček ◽  
Stanislav Staněk ◽  
Martin Ptáček ◽  
Luděk Stádník ◽  
...  

The aim of this study was to evaluate the influence of udder and legs formation on longevity traits (average lactation at culling, lifelong production) and milk production (maximum amount of milk per lactation, fat content in % and kg, protein content in % and kg) in Czech Fleckvieh, Montbeliarde and their crossbreds. Total 2725 culled Czech Fleckvieh, Montbeliarde cows, and their crossbreeds from two farms were put into dataset. Statistical analysis was performed by SAS 9.3 program. The model equation contained effect of breed, housing, legs formation, and udder formation. The groups for legs (<74.61 points, 74.61 – 81.90 points, >81.90 points) and udder (<76.94 points, 76.94 – 81.75 points, >81.75 points) formation were created according arithmetic means and standard deviation. The best results for longevity were in legs formation parameters achieved in group <74.61 points. On the other hand, the best results for milk yield parameters were in legs formation achieved in group >81.90 points. In evaluation udder formation groups were the best and significant (P < 0.05 – 0.01) results for longevity and milk production achieved in group >81.75 points. Only parameters fat and protein percentages were better in group <76.94 points according udder formation. Generally, we can conclude that udder formation was more important than legs formation. From the results we can also conclude that cows with correct exterior do achieve higher longevity and milk production. This confirmed the importance of choosing cows for breeding purposes not only with high potential for milk production, but also for correct exterior parameters and formation.


2010 ◽  
Vol 55 (No. 5) ◽  
pp. 195-199 ◽  
Author(s):  
I. Kowalewska-Łuczak ◽  
H. Kulig ◽  
M. Kmieć

The aim of this study was to investigate associations between PPARGC1A genotypes and haplotypes and milk production traits. The study included 181 Jersey cows. The genotypes were identified by the PCR-RFLP method. The frequencies of the most common alleles were as follows: T &ndash; 0.63 (c.1892T&gt;C) and A &ndash; 0.88 (c.3359A&gt;C). The frequency of the most common haplotype was TC/AA &ndash; 0.558. The results showed that there were no statistically significant associations between the individual genotypes of both SNPs and milk traits; however, individuals with the CC/AC haplotype produced significantly (P &le; 0.05; P &le; 0.001) less milk and had a significantly (P &le; 0.001) higher protein content in milk.


2016 ◽  
Vol 52 ◽  
pp. 109-114
Author(s):  
K. O. Skoryk

The aim of the work was to study the basic productive traits of Saanen goats of Latvian selection in the order of their selection value: milk yield, fat and protein content in milk. These traits were investigated in the daughters from different goats-fathers. Materials and methods of research. The study was conducted at "Grandmother’s Goats" breeding farm, Galaiky village, Tetiiv district, Kyiv region on Saanen goats of Latvian selection. From mid-spring to mid-autumn the goats grazed and were kept in a corral. In winter, the goats were indoors on deep litter. Milking was twice a day into milk pipeline, it took place in the milking room with 12 seats. The farm is provided enough by coarse, succulent and concentrate feeds. It is considered that the winter diet of dairy goats is hay, twigs, concentrate feed, root vegetables, and mineral supplements. The primary livestock data of milk production in 41 goats for seven lactations were considered. The basic selection traits were studied in the order of their breeding value: milk yield, fat and protein content in milk. The same traits were investigated in terms of fathers of milking goats. The obtained results were processed statistically by N. A. Plohinskiy (1970). Results of research. The research results show that milk yield of Saanen goats of Latvian selection varied depending on lactation. It was found that the milk production during the 1st lactation was 565 kg, fat content in milk – 3.2% and protein content – 3.0%. Milk yield per lactation tended to increase – from the 3rd to 6th lactation inclusively, as compared with the 1st and 7th. The maximum level of milk yield was during the 6th lactation. The differences in milk yield between the 6th and the 1st, 2nd, 5th and 7th lactations are significant with the second degree of reliability. Significant coefficient of variation for milk yield (20-35%) gives reason to consider it possible to conduct the selection by this trait. Several other data are presented by T. Orlovskaya. She considers that goats produce maximum milk yield on average after the 3rd parturition. Milking ability of goats after the 5th-6th lactation begins to fall gradually. She also thinks that biological value of milk is reduced with increase in milk yield, namely reduced fat content and, in some cases, changing the taste of milk. Subsequently, the fat content in milk during the 2nd-4th lactations increased by 0.1%, and during the 5th lactation it reached the maximum level – 3.6%. The protein content in the investigated goat milk was small and ranged from 3 to 3.1%. Differences in fat and protein content during all the lactations were unreliable. Ratio of fat content to protein content in Saanen goats’ milk was studied for seven lactations. It was found that the ratio of fat to milk for all the seven lactations did not reach the optimal levels. This is lack of milk quality of the investigated goats. Milk yield, fat and protein content in milk of daughters of four Saanen billy goats were investigated to determine the influence of fathers on the daughters’ productivity. Analysis of milk productivity data for daughters of goats Amors 046062340091, Wicks 030810040238, Priers 0460623640427 and Frends 038028540074 showed that the highest milk yield was in daughters of Amors 046062340091 – 622 kg. Several less milk yield (590 kg) was in the daughters of Frends 038028540074, and the lowest milk yields were observed in daughters of Wicks 030810040238 and Priers 0460623640427. Thus, daughters’ milk yields depend essentially on fathers. At the same time the fat and protein content in the milk of daughters from different fathers is almost identical. This should be taken into account at matching billy goats for breeding stock of goats. In our research of milk yield of daughters from different billy goats there was a significant difference, but protein and fat content in daughters’ milk almost was no different. Thus, the findings don’t always coincide with the data given in the literature. This concerns with the milk production of Saanen goats and its quality (fat and protein content). Earlier, we have shown that Saanen goats’ milk had a little lower density than in Lamancha, Russian, Nubian, Alpine and Megrelian goats. Protein content was at level of 3.85%, or 1.4% greater than in Russian goats’ milk, but 0.15% less than in Lamancha, 1.23% less than in Nubian, 0.4% – in Alpine, and 1.65% ­– in Megrelian goats’ milk. The fat content in goat milk of the compared breeds was also the lowest (by 1.85% than in Lamancha, by 0.79% than in Russian, by 0.97% than in Nubian, by 0.57% than in Alpine and 1.12% than in Megrelian breeds). Also, content of dry milk residue was low (0.67-1.48% less). Despite these differences in milk yield of goats, research in this area should be expanded in order to determine the prospects for further goat breeding of certain breeds. Conclusions. Milk yield per lactation tended to increase – from the 3rd to 6th lactation inclusively, as compared with the 1st and 7th. The maximum level of milk yield was during the 6th lactation. Significant coefficient of variation for milk yield (20-35%) gives reason to consider it possible to conduct the selection by this trait. The differences in the level of milk production of daughters from different goats were revealed that should be taken into account at matching billy goats for breeding stock. In Ukraine goats’ milk yield of different breeds requires further study.


2007 ◽  
Vol 2007 ◽  
pp. 7-7
Author(s):  
Sonia Zakizadeh ◽  
Monika Reissmann ◽  
Peter Reinecke ◽  
Ghodrat Rahimi ◽  
Ardeshir Nejati-Javaremi ◽  
...  

Beta-Lacto globulin (BLG) is a major protein in the milk of ruminants. Bovine BLG gene is located on the chromosome 11q28 and has two common genetic variants, ‘A’ and ‘B’ (Gene-Bank X14710). These variants are different in amino acids at positions 64 and 118, where allele ‘A’ has Asp and Val and allele ‘B’ has Gly and Ala, respectively. Difference at position 118 can be detected by PCR-RFLP (Medrano and Agiular-Cordova 1990). It is reported that allele ‘A’ is associated with milk production (Strzalkowska et al. 2002) and allele ‘B’ has a positive effect on fat percentage (Tsiaras et al. 2005). The aim of our study was to estimate the allelic frequency in polymorphic site of exon IV (allele ‘A’ or HaeIII (-)) of BLG gene in Holstein dairy cattle of Iran and also to find any association of total milk production (first lactation) with BLG genotypes.


2019 ◽  
Vol 62 (2) ◽  
pp. 413-422 ◽  
Author(s):  
Adel H. M. Ibrahim ◽  
Nikolaos Tzanidakis ◽  
Smaragda Sotiraki ◽  
Huitong Zhou ◽  
Jonathan G. H. Hickford

Abstract. The aim of this study was to estimate the effect of variation in the fatty acid binding protein 4 gene (FABP4) on milk production traits in Greek Sfakia sheep. Polymerase chain reaction – single-stranded conformational polymorphism (PCR-SSCP) analysis was used to genotype a total of 374 Sfakia ewes for two regions of FABP4 located around exon 2–intron 2 (Region 1) and exon 3–intron 3 (Region 2). Each month, for a period of 6 months, milk samples were collected from the ewes to measure total milk yield, fat content, protein content, lactose content, non-fat solid content, pH, and somatic cell count (SCC). A general linear model was used to test the association between the variation observed in FABP4 and milk production traits. Four gene variants (A1–A4) were found in Region 1 and two variants (C1–C2) were found in Region 2. In the first region, the FABP4 genotype significantly affected (P<0.05) non-fat solid levels, fat content, and SCC. The presence of the A2 variant was significantly associated (P<0.05) with decreased SCC, while the presence of A4 was significantly associated with decreased milk yield (P<0.01), increased non-fat solid content (P<0.05), decreased fat content (P<0.01), increased lactose content (P<0.05), and increased pH (P<0.05). In the second region, FABP4 genotype had an effect (P<0.05) on protein content and the presence of the C2 variant was associated (P<0.05) with increased protein content, decreased SCC, and lower pH. The results suggest an association between variation in ovine FABP4 and milk production traits in Greek Sfakia sheep. Nevertheless, further analyses in independent sheep populations of increased size will strengthen these findings.


Mljekarstvo ◽  
2021 ◽  
Vol 71 (3) ◽  
pp. 194-203
Author(s):  
Khaoula Attia ◽  

In Tunisia, dairy farming faced several problems limiting the improvement of the milk sector, mostly at the level of small farmers. These difficulties are related to the milk quality affected by the feed quality distributed throughout the year. This study aimed to evaluate the effect of rations and seasons on daily individual milk production (DIMP) and some physicochemical characteristics (Fat content: FC, Protein content: PC, Urea concentration: UC, Total Solids: TS). A survey and milk samplings were performed on 135 farmers. Collected data were treated by the variance analysis using the GLM procedure of the SAS system. This study allowed to identify four types of ration: TR1: Concentrate (CC)+Green Forage(GF), TR2: CC+Dry Forage (DF), TR3: CC+GF+DF and TR4: Diet containing Silage (S), used by farmers with the frequencies of 14.82, 37.19, 44.6 and 3.38 % respectively. The highest DIMP (P<0.0001) was obtained by TR1 (17.3 kg/day) and the lowest (13.6 kg/day) by TR2. TR4 allowed FC, PC (P<0.05) and TS (P<0.01) higher (3.84, 3.14 and 12.31 % respectively) than those obtained in the other rations. The UC (P<0.0001) was the highest in TR1 and TR3 (32.05 mg/dL) followed by TR4 (28.3 mg/dL) and TR2 (26.2 mg/dL). Concerning the season effect, the highest DIMP (P<0.0001) was obtained during spring (17.7 kg/day), the lowest was during autumn (13.1 kg/day). The FC (P<0.05) was the lowest in winter (3.52 %). The PC (P<0.01) was higher during autumn and winter (averaged 3.07 %). The UC (P<0.0001) was the highest in spring (32.23 mg/dL) and the lowest in autumn (25.67 mg/dL). It was concluded that milk parameters were in the acceptable ranges comparatively to the national averages.


Author(s):  
Yelena Boyko ◽  
Vladimir Rossokha ◽  
Oksana Drobyazko ◽  
Yelyzaveta Oliinychenko ◽  
Elena Zaderikhina

In order to determine the productivity of Simmental cattle population, genotyping of animals by genes associated with economically useful traits was carried out. The application of results in planning long-term selection and breeding work, considering genotypes of breeding bulls was proposed. Polymorphism of kappa-casein (κ-Cn), beta-lactoglobulin (βLG), leptin (LEP) and somatotropin (GH) genes in Simmental cattle population (The breeding facility “Krasniy Velikan”) was performed using of PCR-RFLP method. The level of productivity on milk yield, fat and protein content in milk was established. The combination of genotypes in the Simmental cattle population was determined on the basis of marking hereditary information by genes of quantitative traits to increase the productive potential of animals. In the studied population of Simmental breed polymorphisms were found to be polymorphic in all studied genes. The connection of controlled genes with indicators of animal milk productivity is established. Genotypes BB (κ-Cn), AA (βLG), BA (GH) and AB (LEP) were characterized by the highest rates of milk yield during the first lactation, genotypes AB (κ-Cn), AA (βLG), BA (GH) and AA (LEP) – the highest fat content in milk, genotypes AA (κ-Cn), BB (βLG), BA (GH) - the highest protein content in milk. The analysis of associative connections of the main productive traits of Simmental cattle with polymorphism of genes of kappa-casein, growth hormone, leptin and beta-lactoglobulin makes it possible to establish the most desirable complex genotypes: κ- to increase the fat content of milk, κ-CnAAGHBALEPAA / ABΒlgBA in selection to increase the protein content in milk. It is shown that in order to increase the productivity of Simmental cattle populations it is necessary to genotype breeding animals by genes associated with economically useful traits and apply the results when planning long-term plans for breeding work considering the genotypes of breeding bulls.


Author(s):  
Jiří Bezdíček ◽  
Jan Šubrt ◽  
Radek Filipčík

The objective of the present study was to explore the effect of the level of inbreeding on milk efficiency of cows and on the content of milk components (fat and protein) in the 1st standard lactation period (305 days). The level of inbreeding (Fx) of inbred cows ranged between 1.25 and 12.5%. Efficiency parame- ters of inbred cows were compared with their contemporaries – half-sisters after the father (n=2567), raised on the same farm whose first lactation ended in same year. Out of the total database (567,036 cows) created in 1980–2002 we incorporated 885 inbred cows. To allow detailed analyses the inbred cows were divided into 3 groups according to the coefficient Fx (1.5–2.3%, 3.0–5.0% and 8.0–12.5%). The database was evaluated using the analysis of variance with 8 fixed effects including the commercial type, own breeding value and breeding value of mother, average efficiency of mother, reproduction parameters (year and age at calving and interval between the 1st and 2nd lactation) and total number of lactations. The statistical programme SAS 8.2 – GLM procedure (Copyright c 1999–2001 by SAS Institute Inc., Cary, NC, USA) was used for statistical analyses. Compared to their contemporaries the 323 kg reduction of milk production of inbred cows with the lowest value of Fx (1.5–2.3%) was insignificant; the decrease in the relative content of fat and protein was insignificant by 0.01 and 0.02%, respectively. Comparisons between contemporaries and inbred cows with Fx at the level of 3.0–5.0% showed a highly significant 407 kg reduction in milk efficiency. The fat content in milk of inbred cows increased by 0.11% (p<0.01) and the protein content by 0.03% (p<0.05) compared to their contemporaries. The reduction in milk production of inbred cows with the highest level of Fx (8.0–12.5%) was significant, i.e. –572 kg; the fat content increased insignificantly by 0.02%, while the protein content was the same in inbred cows and contemporaries. Comparisons of the milk production of inbred cows at various levels of Fx, taking into consideration the fixed effects, showed that the differences were most marked between groups Fx=1.5–2.3% and Fx=8.0–12.5%; the reduction in milk efficiency was –731 kg. A Fx coefficient higher by 1% reduced the milk efficiency of cows by 90.07 kg, while the fat and protein content increased by 0.001% and 0.002%, respectively.


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