scholarly journals ASSOCIATIVE RELATIONSHIPS OF KAPPA-CASEIN, BETA-LACTOGLOBULIN, LEPTIN AND SOMATOTROPIN GENOTYPES WITH DAIRY PRODUCTIVITY IN SIMMENTAL BREED

Author(s):  
Yelena Boyko ◽  
Vladimir Rossokha ◽  
Oksana Drobyazko ◽  
Yelyzaveta Oliinychenko ◽  
Elena Zaderikhina

In order to determine the productivity of Simmental cattle population, genotyping of animals by genes associated with economically useful traits was carried out. The application of results in planning long-term selection and breeding work, considering genotypes of breeding bulls was proposed. Polymorphism of kappa-casein (κ-Cn), beta-lactoglobulin (βLG), leptin (LEP) and somatotropin (GH) genes in Simmental cattle population (The breeding facility “Krasniy Velikan”) was performed using of PCR-RFLP method. The level of productivity on milk yield, fat and protein content in milk was established. The combination of genotypes in the Simmental cattle population was determined on the basis of marking hereditary information by genes of quantitative traits to increase the productive potential of animals. In the studied population of Simmental breed polymorphisms were found to be polymorphic in all studied genes. The connection of controlled genes with indicators of animal milk productivity is established. Genotypes BB (κ-Cn), AA (βLG), BA (GH) and AB (LEP) were characterized by the highest rates of milk yield during the first lactation, genotypes AB (κ-Cn), AA (βLG), BA (GH) and AA (LEP) – the highest fat content in milk, genotypes AA (κ-Cn), BB (βLG), BA (GH) - the highest protein content in milk. The analysis of associative connections of the main productive traits of Simmental cattle with polymorphism of genes of kappa-casein, growth hormone, leptin and beta-lactoglobulin makes it possible to establish the most desirable complex genotypes: κ- to increase the fat content of milk, κ-CnAAGHBALEPAA / ABΒlgBA in selection to increase the protein content in milk. It is shown that in order to increase the productivity of Simmental cattle populations it is necessary to genotype breeding animals by genes associated with economically useful traits and apply the results when planning long-term plans for breeding work considering the genotypes of breeding bulls.

2020 ◽  
Vol 5 (86) ◽  
pp. 107-114
Author(s):  
А.I. Shendakov ◽  
◽  
◽  

The share of genes of Holstein breed was 76.8% in the herd. It was found that during the research period, the normal distributions of milk productivity traits were observed in the herd. The number of cows in the herd decreased, but their breeding qualities increased. The main reason for which animals were culled from the herd was their gynecological diseases (in some years, up to 175 to 188 heads were culled). The protein content in milk varied from 2.90 to 3.39%. Most cows produced milk in which the protein content was at a level of 3.00 to 3.19%. The herd showed positive correlations between the yields of mothers and daughters for the 305-day-long period: 0.234 for the whole herd, 0.517 for the group of cows with 50% of Holstein breed (HF) genes, 0.098 for the group with 51-75% of HF genes, and 0,189 for the group with minimum 76% of HF genes. For the herd, the heritability coefficient for milk yield for the 305- day-long period (h2) was 0.468. The percent of the protein content in milk produced by cows with 50% of Holstein genes had a high heritability coefficient of 0.668. Only cows of the M. Chieftain line showed positive correlations between the fat content in milk produced by mothers and daughters, and positive correlations between the protein content in milk produced by cows of the W. B. Ideal and M. Chieftain lines was found. With an increase in percent of Holstein breed genes (HF) from 25 to 75% and more, there were no significant differences in milk yield among first-calf cows (they amounted to 5,424 ± 83 – 5,520 ± 116 kg of milk). The percentage of fat and protein in milk for the whole herd was positively interconnected (r = + 0.460). On average, daughters exceeded their mothers by 23.3% in terms of milk yield and by 2.0% in terms of the percentage of fat content in milk (of the trait value). The quantity of milk fat and protein increased by 25.8 and 22.5%, respectively. Based on the research results, it has been concluded that the Holstein breed has a positive effect on the productive traits of dairy cattle, but technology optimization is required to continue selection activities.


2018 ◽  
Vol 51 ◽  
pp. 124-131
Author(s):  
О. P. Plivachuk ◽  
T. M. Dyman

The objective of this work was to study the effect of phenotypic combination of genetic variants α-LA and β-LG on the content of technologically and nutritionally important components of milk in cows of Ukrainian Black-and-White Dairy breed. Method PCR-RFLP was used to identify cow’s genotypes on the indicated genes. Milk yield was calculated based on the results of monthly control milking. It has been determined such indices of cow’s milk: fat content, protein content, total solids content, milk solids non-fat (MSNF) content, lactose content, casein content, casein number, milk density, milk rennet ability, heat stability (alcohol number) of milk. Statistical analysis was carried out using program Statistica 6.0. The study of genetic structure of the herd of Ukrainian Black-and-White Dairy cattle showed the high level of polymorphism on α-LA and β-LG genes. 6 genotypes were identified. Genetic structure of the herd on two genes simultaneously was determined. 9 complex genotypes were revealed. Phenotypic variants α-LA АВ/β-LG АВ, α-LAАА/β-LG АА and α-LAАВ/β-LG АА had the highest frequency – 26, 21,5and 14 % respectively. Frequencies of remaining genotypes were not higher than 10 %. The highest milk yield (5617 kg) was received from cows with genotype α-LA ВВ/β-LG АА. The percentage of these animals in the herd was only 7,5 %. The lowest milk yield (5367 kg) was fixed for the group of animals with complex genotypeα-LA АА/β-LG ВВ. The frequency of this genotype was 7 %. Three the most distributed complex genotypes (61,5 %) characterized by medium features of milk yield. Cows with genotypes α-LAАВ/β-LGВВ had the highest indices of fat content (3,93 %). The percentage of these animals in the herd was 5 %. The difference between cows’ groups with the highest and the lowest indices of fat content was 0,14% (td=2,5; p<0,05). Cows with genotypes α-LAАВ/β-LG АА had the highest protein content. Their percentage in the herd was 14 %. The difference between cows’ groups with highest and lowest indices of protein content was 0,06 % (td=1,1; p<0,05). The highest indices of casein content and casein number (2,42 % and 78,3 % respectively) were observed in animals with complex genotypes α-LAВВ/β-LG АВ andα-LAВВ/β-LGВВ, their frequency in the studied herd was 11 %. The difference between cows’ groups with highest and lowest above indicators was 0,05 % (td=1,8; p<0,05) and 3 % (td=2,38; p<0,001) respectively. Thus, milk from 11 % of cows of the studied herd of Ukrainian Black-and-White Dairy breed complied with the requirements for cheese manufacture better. Indicator of rennet ability confirmed this. The lowest time of milk coagulation (rennet ability) we observed in milk from cows with complex genotype α-LAВВ/β-LGВВ – 27,7 min. We can assume that expression of alleles B of α-LA and β-LG in this phenotypic combination determines such desirable properties for cheese making. At the same time, milk from cows with complex genotypes α-LAВВ/β-LG АВ and α-LAВВ/β-LGВВ had the worst indices of heat stability, its alcohol number was 2,17 ml. High heat stability of milk was determined by expression of A allele of genes α-LA and β-LG in phenotypic combination α-LA/β-LG. Milk from cows with genotype α-LA АВ/β-LG АВ had the highest lactose content (4,53 %). The difference between cows’ groups with highest and lowest indices of lactose content was 0,06% (td=1,1; p<0,05). Significant differences between the cows of different complex genotypes were not found for the total solids content. At the same time, some differences on MSNF content were observed. The highest indices (8,65 %) were revealed in groups of animals with genotypesα-LAАА/β-LG АА andα-LAВВ/β-LG АА, lowest (8,57 %) – with genotype α-LAАВ/β-LGВВ, but these differences were not statistically significant. The result presented here shows the relationship between the combination of alfa-lactalbumin and beta-lactoglobulin genetic variants in Ukrainian Black-and-White Dairy cattle and the productivity traits of animals, technological properties of their milk.


Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.


1982 ◽  
Vol 49 (1) ◽  
pp. 25-28 ◽  
Author(s):  
David G. Chamberlain ◽  
Phillip C. Thomas

SUMMARYEight cows in mid lactation and receiving a diet of perennial ryegrass silage and barley (70:30 on a DM basis) were given intravenous supplements of l-methionine (8 g/d). The methionine treatment had no significant effect on milk yield, protein content or lactose content, but increased milk fat content and yield by approximately 10% (P < 0·05).


2021 ◽  
Vol 1 (2) ◽  
pp. 29-35
Author(s):  
N. I. Abramova ◽  
◽  
O. L. Khromova ◽  
M. O. Selimyan ◽  
◽  
...  

The use of foreign-bred bulls leads to changes in the genetic structure of the black-and-white breed population, which affects the level of population characteristics. The study was conducted to study the comparative variability and heritability of productive and reproductive characteristics of daughters of sires of domestic and foreign selection in pedigree livestock 10 250 cows 1st calving modern population of black-motley breed of the Vologda region. The superiority of the offspring of foreign bulls in the average indicators of all the studied traits was established with confidence (P<0,001). There were no significant differences in the level of variability of the characteristics of the descendants of domestic and foreign bulls. According to productive traits, the greatest variability of indicators was established for milk yield for 305 days of the 1st lactation Cv = 17,3 – 17,7%, therefore, selection on this trait will be more effective. The greatest share of the influence of bullsfathers, both domestic and foreign selection, in the total sum of all factors determining the value of traits in the population of the black-and-white breed, is determined by the milk yield of daughters η2 = 0,22 – 0,24, the age of the 1st fruitful insemination and the first calving η2 = 0,24 – 0,25. In further breeding work with the breed, it is necessary to obtain and use domestic producers that exceed the breeding value of foreign bulls.


2020 ◽  
Vol 36 (4) ◽  
pp. 417-426
Author(s):  
Dragan Niksic ◽  
Vlada Pantelic ◽  
Dusica Ostojic-Andric ◽  
Predrag Perisic ◽  
Ljiljana Samolovac ◽  
...  

In order to examine the variability of production performance traits (lactation duration, milk yield for the whole lactation, milk yield in standard lactation, milk fat content, milk fat yield in standard lactation, protein content and yield in standard lactation), 954 cows, domestic and imported Simmental populations, were included in the study, with a total of 3641 completed lactations. Cows were located in the area of Toplica district, reared on individual farming households (tied system) and on the farm with intensive farming (free system). Based on the rearing method and origin, animals were divided into four groups: Group 1 (domestic animals reared by individual agricultural producers on individual farming households); Group 2 (imported animals reared by individual agricultural producers on individual farming households); Group 3 (domestic animals reared on the farm) and Group 4 (imported animals reared on the farm). The fourth group of studied cows showed the highest yield and protein content, while the cows of the third group had the longest lactation and the highest milk fat content. All production performance traits varied highly significantly (p?0.001) under the influence of the combined factor of housing/rearing method and origin.


Author(s):  
Lina Karlova ◽  
◽  
Olena Lesnovska ◽  
Roman Mylostyvyi ◽  
◽  
...  

The aim of the research was to evaluate the influence of the type of the nervous system on the productive and reproductive qualities of animals. The type of nervous system (strong, weak) of cows of the Ukrainian red dairy breed was determined by the index of the nervous system, which is based on the variability of the fat content in the morning milk yield after changing the conditions of cows keeping. Under the new conditions of keeping cows with a strong type of nervous activity, the coefficients of variability of the morning milk yield and the fat content in milk practically remained at the same level (their slight increase was noted at the level of 4.5 and 3.1%). In cows of a weak type under similar conditions of keeping, a significant increase in the variability of milk yield (by 53.6%) and fat content in milk (by 266.7%) was found. In animals of both types, in the first days of summer-camp keeping, in comparison with winter-stall keeping, there was an increase in milk yield and a decrease in fat content in milk. In cows of the strong type, the fat content decreased by 0.08% and of the weak type by 0.12%. The average index of the nervous system type in animals of the strong type is 1.18, and in cows of the weak type 2.71 (P> 0.999). Before and during milking, the pulse rate in the cows of weak type was higher by 8.0 and 10.8 beats / min. (P> 0.999), compared with the strong type. The animals of strong type of the nervous system, in comparison with the weak type, had a smaller thickness of the concha and skin, a higher pulse and respiratory rate, and a lower rectal body temperature. In cows of a strong type of the nervous system in postnatal ontogenesis, the formation of productive traits took place more rapidly and they better adapted to the conditions of the external environment. This is evidenced by their high coefficient of relative decline in the growth at the age of one (85.0) than in animals with a weak type of nervous system (78.5). The advantage of cows with a strong type of nervous activity over a weak one in milk yield was 348 kg (P>0.95) and in milk fat 8.0 kg. The service period in animals with a strong type of nervous activity was 6.3 days shorter. The animals with a strong type of nervous activity during the first lactation had a higher adaptation index compared with animals of a weak type. The difference for this indicator is 0.49 (or 3.7%). They also had a higher productivity index for the first (3.1%) and second (4.0%) lactation. Thus, the typological features of the nervous activity of cows affect the formation of the most important functions of the body, which can be used in breeding work to improve the herd.


Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


2008 ◽  
Vol 52 (No. 4) ◽  
pp. 83-87 ◽  
Author(s):  
A. Matějíček ◽  
J. Matějíčková ◽  
E. Němcová ◽  
O.M. Jandurová ◽  
M. Štípková ◽  
...  

The objective of this study was to estimate the joint effects of <i>CSN3</i> and <i>LGB</i> genotypes on breeding values of milk production parameters. <i>CSN3</i> (kappa-casein) and <i>LGB</i> (beta-lactoglobulin) genotypes of 120 Czech Fleckvieh sires were detected using the PCR-RFLP method. Breeding values of sires were obtained from the Official Database of Progeny Testing. Ten genotype combinations were detected. Genotypes <i>ABAB</i> (25.0%), <i>ABAA</i> (13.3%) and <i>ABBB</i> (13.3%) were the most frequent. Significant effects of genotype combinations on breeding values for fat and protein content were found. The highest breeding values for milk (+621 kg) and protein (+15.8 kg) yields were associated with genotype combination <i>ABAA</i>, while the highest breeding values for content parameters (+0.15% for protein content and +0.55% for fat content) were associated with genotype combination <i>BBAB</i>.


1967 ◽  
Vol 39 (3) ◽  
pp. 156-165
Author(s):  
Vappu Kossila ◽  
Onerva Hintikka

Coefficients of simple and partial correlations between the milk yield and the percentages of fat and protein in milk were calculated separately for the first-calvers and older cows during the early period of lactation (5—39 days post partum), during a later period (40—360 days p.p.), and during the entire period of lactation, from the data of 1028 milk samples obtained during the indoor-feeding period from 70 different Ayrshire cows belonging to the Viik Experimental Farm dairy herd. Statistical treatment of the data revealed that the milk yield affected the protein content of the milk more than its fat content, especially in the first-calvers. The protein-fat correlation, which was somewhat affected by the milk yield, was generally less close during the early stage of lactation than during its later stage. The percentage of variation in a) the milk yield that was explained by the variations in the percentage of fat and protein, was affected more by age than by the stage of lactation, b) the fat content of milk that was explained by the variations in the milk yield and the protein content of milk, was influenced more by the stage of lactation than by the age of the cow, c) the protein content of milk that was explained by the variations in milk yield and the fat content, was influenced by age as well as by stage of lactation. The equations for estimating theoretically the daily milk yield, the percentage of fat, or the percentage of protein in milk, when two out of the three variables are known, have been presented separately for the first-calvers and for the older cows at early and at later stages as well as during the entire period of lactation. The results of the study indicated that the milk yields and the percentages of fat and protein in milk vary to such an extent independently of each other, even when the age of cows, the stage of lactation, and the season of the year have been taken into consideration, that none of the dependent variables in question could be very closely estimated on the basis of knowing two of the independent variables.


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