scholarly journals Relationship between abnormal spermatozoa and seminal plasma free amino acids in boars

2008 ◽  
Vol 52 (No. 2) ◽  
pp. 44-49 ◽  
Author(s):  
J. Čeřovský ◽  
S. Frydrychová ◽  
A. Lustyková ◽  
M. Rozkot
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Seminal Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sperm Concentration ◽  
Negative Relationship ◽  
Total Content ◽  
Group A ◽  
Plasma Free Amino Acids

The objective of this study was to provide some data on concentrations of seminal plasma free amino acids (FAAs) in order to compare these values with different total contents of morphologically abnormal spermatozoa (MAS) in the semen of 37 experimental adult boars. The boars were kept in the same housing, feeding and breeding conditions. Immediately after collection, semen gel free volume, sperm concentration and total MAS were determined microscopically. The boars were divided into two groups (A &minus; <i>n</i> = 24, B &minus; <i>n</i> = 13) according to the significantly different total MAS content (<i>x</i>: A = 9.46 &plusmn; 4.95, B = 46.00 &plusmn; 14.54%, <i>P</i> < 0.01). Deproteinized seminal plasma samples (supernatant) were analysed for concentrations of 13 FAAs: Tau, Asp, Thr, Ser, Glu, Gly, Ala, Val, Met, Ile, Leu, Tyr and Lys by liquid chromatography (AAA 339 M analyser). A highly significant correlation was determined between total MAS and total FAA content in all experimental boars (<i>n</i> = 37, <i>r</i> = &minus;0.60, <i>P</i> < 0.01). Glutamic acid was a predominant FAA in seminal plasma in both groups of boars (x: A = 28.49 vs. B = 17.60&mu;M/100 ml) but its concentration was significantly higher in group A (<i>P</i> < 0.01). The proportion (%) of glutamic acid concentration in the total content of FAAs in both groups of boars was nearly equal (A = 38.1 vs. B = 38.9%, <i>P</i> > 0.05) as well as the proportion index of the individual FAAs calculated from glutamic acid (= 100%). The differences in the particular FAAs recorded between group A and group B were statistically significant for 11 out of the 13 FAAs observed (<i>P</i> < 0.05 and <i>P</i> < 0.01) in favour of group A as well as in the total calculated content of FAAs per boar (x: 74.70 vs. 45.23&mu;M/100 ml, <i>P</i> < 0.01). A significantly negative relationship between the MAS content and the concentration of seminal plasma FAAs (<i>r</i> = &minus;0.60, <i>P</i> < 0.01) is the main result of this study with regard to the markers for potential boar semen fertility estimation.

Analytische Untersuchungen des Wehrsekretes von Peripatopsis moseleyi (Onychophora) / Analytical Investigations of the Defensive Secretion from Peripatopsis moseleyi (Onychophora)

1977 ◽  
Vol 32 (1-2) ◽  
pp. 57-b ◽  
Author(s):  
Harald Röper
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Defensive Secretion ◽  
Gel Filtration ◽  
Total Content ◽  
Gas Liquid Chromatography ◽  
Isoleucine Leucine

The defensive secretion of Peripatopsis moseleyi (Onychophora) consists of 84% water and 16% protein and free amino acids. The secretion’s defensive effectiveness is an anti-predator “sticking” action. The secretion is flung out of the oral papillae in liquid state. It is then denaturized by the air and develops increasingly sticky white threads, probably through the devel­opment of disulfide bridges from the protein content. The elastic properties of the secretion threads indicate a micellar structure. The defensive secretion contains no volatile organic components or carbohydrates. This was confirmed by gas- liquid chromatography and thin-layer chromatography. After acidic hydrolysis of the secretion the following amino acids were determined quantita­tively: aspartic acid, threonine, serine, proline, glutamic acid, glycine, alanine, valine, cysteine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine and arginine. A “rare” amino acid was not identified. Tryptophane was not present (basic secretion hydrolysis). The quantita­tive determination of free amino acids, based on the total content, showed the following results: glycine (40.9%), glutamic acid (10.8%), aspartic acid (2.65%), lysine (1.3%). This result shows, that the secretion is stored in a watery glycine/glutaminic acid buffer in the oral papillae of Peripatopsis moseleyi. High voltage paper electrophoreses and gel filtration experiments with dextran and agarose gels showed, that the secretion protein consists of, at least, two fractions with different molecular weight.

scholarly journals Assessment of seminal plasma free amino acids among fertile men

2019 ◽  
Vol 2 (1) ◽  
pp. 43-46
Author(s):  
Marwa O. Aboelwafa ◽  
Tahia H. Seleem ◽  
Hassan M. Ibraheem ◽  
Mohammed H. Hassan
Keyword(s):  
Amino Acids ◽  
Seminal Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Free Amino Acids

scholarly journals Compositional changes of proteins and amino acids in germinating coffee seeds

2000 ◽  
Vol 43 (3) ◽  
pp. 259-265 ◽  
Author(s):  
Milton Massao Shimizu ◽  
Paulo Mazzafera
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Total Content ◽  
Total Soluble Protein ◽  
Sds Page ◽  
Compositional Changes ◽  
Quantitative Changes

Endosperm is the main reserve tissue in coffee seeds. Coffee (Coffea arabica L.) seeds were germinated for six weeks and qualitative and quantitative changes in amino acids and proteins were investigated. The total content of free amino acids were reduced during germination, however, protein content remained constant. SDS-PAGE profiles showed that legumin-like proteins became less stained in the last weeks. Asparagine, glutamic acid, aspartic acid, alanine and lysine were the major free amino acids, although serine and glutamine were also significant. Except for tyrosine, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed glutamic acid/glutamine and glycine as the main amino acids. However, other amino acids such as leucine, aspartic acid/asparagine, alanine, lysine, serine were also found in reasonable amounts.

The Level of Plasma Free Amino Acids in Children with Acute Diseases (Part I)

1974 ◽  
Vol 16 (1) ◽  
pp. 54-54
Author(s):  
Teruo Honda ◽  
Naoya Morishima ◽  
Tetsuhiko Matsuno ◽  
Toshihiko Sasaki
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Free Amino Acids

scholarly journals Association of plasma free amino acids with hyperuricemia in relation to diabetes mellitus, dyslipidemia, hypertension and metabolic syndrome

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
MH Mahbub ◽  
Natsu Yamaguchi ◽  
Hidekazu Takahashi ◽  
Ryosuke Hase ◽  
Yasutaka Ishimaru ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Metabolic Syndrome ◽  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Free Amino Acids

scholarly journals Utilization in vivo of glucose and volatile fatty acids by sheep brain for the synthesis of acidic amino acids

1966 ◽  
Vol 101 (3) ◽  
pp. 591-597 ◽  
Author(s):  
R M O'Neal ◽  
R E Koeppe ◽  
E I Williams
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Specific Activity ◽  
Tricarboxylic Acid Cycle ◽  
Sheep Brain ◽  
The Brain

1. Free glutamic acid, aspartic acid, glutamic acid from glutamine and, in some instances, the glutamic acid from glutathione and the aspartic acid from N-acetyl-aspartic acid were isolated from the brains of sheep and assayed for radioactivity after intravenous injection of [2-(14)C]glucose, [1-(14)C]acetate, [1-(14)C]butyrate or [2-(14)C]propionate. These brain components were also isolated and analysed from rats that had been given [2-(14)C]propionate. The results indicate that, as in rat brain, glucose is by far the best precursor of the free amino acids of sheep brain. 2. Degradation of the glutamate of brain yielded labelling patterns consistent with the proposal that the major route of pyruvate metabolism in brain is via acetyl-CoA, and that the short-chain fatty acids enter the brain without prior metabolism by other tissue and are metabolized in brain via the tricarboxylic acid cycle. 3. When labelled glucose was used as a precursor, glutamate always had a higher specific activity than glutamine; when labelled fatty acids were used, the reverse was true. These findings add support and complexity to the concept of the metabolic; compartmentation' of the free amino acids of brain. 4. The results from experiments with labelled propionate strongly suggest that brain metabolizes propionate via succinate and that this metabolic route may be a limited but important source of dicarboxylic acids in the brain.

Plasma free amino acids in the chronically uremic rat

1975 ◽  
Vol 8 (1-6) ◽  
pp. 303-306 ◽  
Author(s):  
A. Mannan ◽  
L.I. Wiebe ◽  
A.A. Noujaim ◽  
D.C. Secord
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Free Amino Acids

scholarly journals Effect of Different Edaphic Crop Conditions on the Free Amino Acid Profile of PH-16 Dry Cacao Beans

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1637
Author(s):  
Quintino Reis de Araujo ◽  
Guilherme Amorim Homem de Abreu Loureiro ◽  
Cid Edson Mendonça Póvoas ◽  
Douglas Steinmacher ◽  
Stephane Sacramento de Almeida ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Free Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Principal Component ◽  
Amino Acid Profile ◽  
Gamma Aminobutyric Acid ◽  
Significant Difference

Free amino acids in cacao beans are important precursors to the aroma and flavor of chocolate. In this research, we used inferential and explanatory statistical techniques to verify the effect of different edaphic crop conditions on the free amino acid profile of PH-16 dry cacao beans. The decreasing order of free amino acids in PH-16 dry cacao beans is leucine, phenylalanine, glutamic acid, alanine, asparagine, tyrosine, gamma-aminobutyric acid, valine, isoleucine, glutamine, lysine, aspartic acid, serine, tryptophan, threonine, glycine. With the exception of lysine, no other free amino acid showed a significant difference between means of different edaphic conditions under the ANOVA F-test. The hydrophobic free amino acids provided the largest contribution to the explained variance with 58.01% of the first dimension of the principal component analysis. Glutamic acid stands out in the second dimension with 13.09%. Due to the stability of the biochemical profile of free amino acids in this clonal variety, it is recommended that cacao producers consider the genotype as the primary source of variation in the quality of cacao beans and ultimately the chocolate to be produced.

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