Evaluation of equine cortical bone transplantation in a canine fracture model

Freeze-dried bovine bone transplantation is commonly used for orthopaedic surgery. Equine bone, which is available in great quantity, can be obtained as easily as bovine bone, and so represents a potential source of bone for transplantation. In the present study freeze-dried equine cortical bones were transplanted into experimentally-induced fibular defects in canines to evaluate xenogenic implantation of equine bone. Cortical bones that had been freed of antigens and defatted with chloroform and methanol were freeze-dried at –80 °C for preservation of bone morphogenetic protein, sterilized with ethylene oxide gas and stored at room temperature. The experimental osteotomy was performed in a 15 mm-long bilateral region of each proximal metaphyseal fibula. The area of defect in eight beagle dogs (n = 16) received a transplanted freeze-dried equine cortical implant. The control group consisting of two beagles dogs (n = 4) received an autograft of a similar implant. The experiment region was radiographically monitored for bone union and host serum osteocalcin level was determined to assess osteoblast activity every two weeks for 24 weeks. In 14 of the 16 experimental cases, the graft was not associated with new bone formation. Resorption after new bone formation and remodelling with new bone formation each occurred in a single case. The results support the potential of using freeze-dried equine cortical bones as a xenogenic bone graft material in canines.

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Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.427 ◽

2006 ◽

Vol 309-311 ◽

pp. 427-432 ◽

Cited By ~ 3

Author(s):

Y. Kim ◽

Y.H. Kown ◽

J.B. Park ◽

J.H. Chung ◽

H.N. Lim ◽

...

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Porous Titanium ◽

Control Group ◽

Bovine Bone ◽

Graft Material ◽

New Bone Formation ◽

Freeze Dried ◽

Custom Made ◽

Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

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Effect of Bio-Oss® Collagen and Collagen Matrix on Bone Formation

The Open Biomedical Engineering Journal ◽

10.2174/1874120701004010071 ◽

2010 ◽

Vol 4 (1) ◽

pp. 71-76 ◽

Cited By ~ 18

Author(s):

R.W.K Wong ◽

A.B.M Rabie

Keyword(s):

Bone Formation ◽

Collagen Matrix ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Parietal Bone ◽

Control Group ◽

Graft Material ◽

New Bone Formation

Objective: to compare the amount of new bone produced by Bio-Oss® Collagen to that produced by collagen matrix in vivo. Method: eighteen bone defects, 5mm by 10mm were created in the parietal bone of 9 New Zealand White rabbits. 6 defects were grafted with Bio-Oss® Collagen. 6 defects were grafted with collagen matrix alone (positive control) and 6 were left empty (negative control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation was made on 100 sections (50 sections for each group) using image analysis. Results: A total of 339% more new bone was present in defects grafted with Bio-Oss® Collagen than those grafted with collagen matrix (positive control). No bone was formed in the negative control group. Conclusion: Bio-Oss® Collagen has the effect of stimulating new bone formation locally compared with collagen matrix in vivo. Bio-Oss® Collagen may be utilized as a bone graft material.

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Evaluation of TCP Loaded Clinoptilolite Use as Graft Material on Rabbit Tibia

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.175 ◽

2011 ◽

Vol 493-494 ◽

pp. 175-180 ◽

Cited By ~ 2

Author(s):

A.N. Uyumaz ◽

L.S. Ozyegin ◽

N. Buyukakyuz ◽

B. Yesilbek ◽

F.N. Oktar

Keyword(s):

Bone Formation ◽

Inflammatory Cell ◽

Cell Formation ◽

Histological Evaluation ◽

Control Group ◽

Graft Material ◽

New Bone Formation ◽

Rabbit Tibia ◽

Dental Applications ◽

Ketamine Hcl

Clinoptilolite mineral which is a member of zeolite family has been recently used in medical and dental applications. Until today, it has never been used as a graft material. In the present study tricalcium phosphate (TCP) imbued 90-95% pure clinoptilolite was used as graft in rabbit tibia. General anesthesia was accomplished using intra muscular (IM) injections of Xylazin and Ketamine HCL. TCP imbued clinoptilolite was placed in the defects created in the tibias of the subjects. Control group defects were left empty. One group was sacrificed on day 28 and the other group on day 56 to evaluate osteogenesis, residual graft material, inflammation and fibrosis. Histological evaluation revealed new bone formation at 28 days to be 14/7 at control group where as its 11/7 for the experimental graft group. At 56 days the values are as 18/7 for control and 14/7 for the experimental graft groups (n = 7). Our study group is the first to perform intrabony application of clinoptilolite. No evidence of abnormal inflammatory cell formation or fibrosis was witnessed in groups. When TCP, which can be used as a standalone graft material, was mixed with zeolite it was not as effective as expected. We believe that, TCP particles are absorbed and captivated in the initial phase and due to absorption strength of zeolite cannot be released back to the environment. We assume its effect can increase with longer time periods. Bone formation without infection is observed around graft particles. Based on the present study, since clinoptilolite does not provoke an inflammatory process, its use in unison with TCP can provide a supporting structure in defects.

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Effectiveness of Anadara granosa shell-Stichopus hermanni granules at accelerating woven bone formation fourteen days after tooth extraction

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v52.i4.p177-182 ◽

2019 ◽

Vol 52 (4) ◽

pp. 177

Author(s):

Rima Parwati Sari ◽

Hansen Kurniawan

Keyword(s):

Bone Formation ◽

Tooth Extraction ◽

Alveolar Bone ◽

Extraction Methods ◽

Control Group ◽

Graft Material ◽

Woven Bone ◽

Freeze Dried ◽

Male Wistar Rats ◽

Anadara Granosa

Background: Post-extraction complications can cause alveolar bone resorption. Hydroxyapatite-tricalcium phosphate (HA-TCP) is one potential bone graft material that can be synthesized from Anadara granosa shell. Another biomarine, Stichopus hermanni, contains hyaluronic acid which can accelerate bone formation on the fourteenth day. Purpose: This study aims to prove the effectiveness of Anadara granosa shell-Stichopus hermanni granules in weaving bone formation fourteen days after tooth extraction. Methods: Twenty-five male Wistar rats were divided into five groups. Their lower left incisor was extracted with gelatin being administered to the control group (C) and granule scaffold derived from Anadara granosa (AG) shell and Anadara granosa shell-Stichopus hermanni at concentrations of 0.4%-0.8%-1.6% (AGSH1-AGSH2-AGSH3) to the treatment group. This study developed a HA-TCP synthesized from Anadara granosa combined with whole Stichopus hermanni to create granule scaffolds by means of a freeze-dried method. The jaw was removed on the fourteenth day post-tooth extraction. Observation of HPA involved the use of an Image Raster®. The resulting data was subjected to analysis by ANOVA and tukey-HSD tests (p<0.05). Results: Data showed the mean of C=0.157±0.078; AG=1.139±0.371; AGSH1=1.595±0.291; AGSH2=1.740±0.308; and AGSH3=1.638±0.286. Statistical analyses showed significant differences in the woven bone area (mm2) between C and the treatment groups AG;AGSH1;AGSH2; AGSH3; and between AG and the AGSH2 groups. Conclusions: Scaffold granules from Anadara granosa shells and Stichopus hermanni effectively accelerate the bone formation process with the most effective being Stichopus hermanni at a concentration of 0.8%.

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Effects of Autogenous Tooth Bone Graft and Platelet-Rich Fibrin in Peri-Implant Defects: An Experimental Study in an Animal Model

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-19-00038 ◽

2020 ◽

Vol 46 (3) ◽

pp. 221-226

Author(s):

Alper Kızıldağ ◽

Ufuk Tasdemir ◽

Taner Arabacı ◽

Canan Aksu Kızıldağ ◽

Mevlüt Albayrak ◽

...

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Dental Implants ◽

Bone Healing ◽

Bone Defects ◽

Control Group ◽

Graft Material ◽

New Bone Formation ◽

Platelet Rich Fibrin ◽

Bone To Implant Contact

The aim of this study was to evaluate the effect of autogenous tooth bone graft (ATBG) combined with platelet-rich fibrin (PRF) on bone healing in rabbit peri-implant osseous defects. Eighteen New Zealand rabbits were divided into 3 groups. Bone defects were prepared in each rabbit, and then an implant cavity was created in the defects. Dental implants were placed, and the peri-implant bone defects were treated with the following 3 methods: no graft material was applied in the control group, bone defects were treated with ATBG in the ATBG group, and bone defects were treated with ATBG combined with PRF in the ATBG+PRF group. After 28 days, the rabbits were sacrificed, and the dental implants with surrounding bone were removed. New bone formation and the percentage of bone-to-implant contact (BIC) were determined with histomorphometric evaluations. New bone formation was significantly higher in the ATBG+PRF group than the control and ATBG groups (P < .05). In addition, BIC was significantly higher in the ATBG+PRF group than in the control and ATBG groups (P < .05). The combination of ATBG with PRF contributed to bone healing in rabbits with peri-implant bone defects.

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Titanium-Reinforced Polytetrafluoroethylene Membrane Combined with Inorganic Polyphosphate Induces Exophytic Bone Formation in Rabbit Calvaria

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.1075 ◽

2007 ◽

Vol 330-332 ◽

pp. 1075-1078 ◽

Cited By ~ 1

Author(s):

J.S. Kim ◽

S.Y. Na ◽

Y.H. Kown ◽

J.B. Park ◽

J.H. Chung ◽

...

Keyword(s):

Statistical Analysis ◽

Bone Formation ◽

Bone Regeneration ◽

Control Group ◽

Bovine Bone ◽

New Bone Formation ◽

Inorganic Polyphosphate ◽

Promoting Effect ◽

Wound Area ◽

Bovine Bone Mineral

The purpose of this study was to examine if the application of titanium-reinforced expanded polytetrafluoroethylene(TR-ePTFE) membrane combined with bovine bone mineral(BBM) soaked in inorganic polyphosphate promotes exophytic bone formation in rabbit calvaria. For this purpose, a total of 8 rabbits were used, and rectangular decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped TR-ePTFE membranes (RPTPMs) were filled with BBM soaked in saline and placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTPMs were filled with BBM soaked in 4%, 8% and 16% inorganic polyphosphate prior to fixing with metal pins. Animals were sacrificed at 4 and 8 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicated that at 8 weeks, all the experimental groups demonstrated exophytic bone formation. At 8 weeks, the 8% polyphosphate group revealed the most new bone formation (p<0.05). On the basis of these findings, we conclude that inorganic polyphosphate has a promoting effect on bone regeneration, possibly by enhancing osteoinductivity of the decorticated wound area and osteoconductivity of the carrier material, but not much as we expected.

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Bone-to-Implant Contact and New Bone Formation Within Human Freeze-Dried Bone Blocks Grafted Over Rabbit Calvaria

The International Journal of Oral & Maxillofacial Implants ◽

10.11607/jomi.5366 ◽

2017 ◽

Vol 32 (4) ◽

pp. 768-773 ◽

Cited By ~ 4

Author(s):

Zvi Artzi ◽

Karen Anavi-Lev ◽

Avital Kozlovsky ◽

Liat Chaushu ◽

Frank Schwarz ◽

...

Keyword(s):

Bone Formation ◽

New Bone Formation ◽

Freeze Dried ◽

Bone To Implant Contact

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The use of solvent-preserved human and bovine cancellous bone blocks for lateral defect augmentation - an experimental controlled study in vivo

Head & Face Medicine ◽

10.1186/s13005-021-00275-1 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Lara Schorn ◽

Tim Fienitz ◽

Kathrin Berndsen ◽

Norbert R. Kübler ◽

Henrik Holtmann ◽

...

Keyword(s):

Bone Formation ◽

Cancellous Bone ◽

Bone Substitute ◽

Bone Matrix ◽

Human Bone ◽

Controlled Study ◽

Bovine Bone ◽

New Bone Formation ◽

Bone Substitute Material ◽

Substitute Material

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

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