Effects of Autogenous Tooth Bone Graft and Platelet-Rich Fibrin in Peri-Implant Defects: An Experimental Study in an Animal Model

2020 ◽  
Vol 46 (3) ◽  
pp. 221-226
Author(s):  
Alper Kızıldağ ◽  
Ufuk Tasdemir ◽  
Taner Arabacı ◽  
Canan Aksu Kızıldağ ◽  
Mevlüt Albayrak ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Dental Implants ◽  
Bone Healing ◽  
Bone Defects ◽  
Control Group ◽  
Graft Material ◽  
New Bone Formation ◽  
Platelet Rich Fibrin ◽  
Bone To Implant Contact

The aim of this study was to evaluate the effect of autogenous tooth bone graft (ATBG) combined with platelet-rich fibrin (PRF) on bone healing in rabbit peri-implant osseous defects. Eighteen New Zealand rabbits were divided into 3 groups. Bone defects were prepared in each rabbit, and then an implant cavity was created in the defects. Dental implants were placed, and the peri-implant bone defects were treated with the following 3 methods: no graft material was applied in the control group, bone defects were treated with ATBG in the ATBG group, and bone defects were treated with ATBG combined with PRF in the ATBG+PRF group. After 28 days, the rabbits were sacrificed, and the dental implants with surrounding bone were removed. New bone formation and the percentage of bone-to-implant contact (BIC) were determined with histomorphometric evaluations. New bone formation was significantly higher in the ATBG+PRF group than the control and ATBG groups (P < .05). In addition, BIC was significantly higher in the ATBG+PRF group than in the control and ATBG groups (P < .05). The combination of ATBG with PRF contributed to bone healing in rabbits with peri-implant bone defects.

Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

2006 ◽  
Vol 309-311 ◽  
pp. 427-432 ◽  
Author(s):  
Y. Kim ◽  
Y.H. Kown ◽  
J.B. Park ◽  
J.H. Chung ◽  
H.N. Lim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Porous Titanium ◽  
Control Group ◽  
Bovine Bone ◽  
Graft Material ◽  
New Bone Formation ◽  
Freeze Dried ◽  
Custom Made ◽  
Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

Effect of Synthetic β-TCP and Bovine-Derived Hydroxyapatite Grafting on Bone Regeneration in Rats

2007 ◽  
Vol 361-363 ◽  
pp. 1261-1264
Author(s):  
C.S. Lim ◽  
S.G. Kim ◽  
Sung Chul Lim
Keyword(s):  
Bone Formation ◽  
Light Microscopy ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Frontal Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Grafting Materials ◽  
Defect Margin

We evaluated the bone healing effect of grafting with synthetic β-tricalcium phosphate (β-TCP; Cerasorb®), bovine-derived hydroxyapatite (HA; Bio-Oss®), and a mixture of β-TCP and HA in rats. Each material was grafted in prepared 8-mm frontal bone defects in 15 rats. The control group underwent surgery without any grafting materials and was examined after 4 weeks, whereas the experimental groups received grafting materials and were examined after 1, 2, and 4 weeks. After implantation, the rats were sacrificed for histomorphometric studies using light microscopy, and the data were analyzed using analysis of variance. Considerable inflammation and fibrosis were observed after 1 and 2 weeks in all experimental groups, whereas the inflammation was reduced and fibrosis was stabilized after 4 weeks. New bone formation was observed at the defect margin. Statistically, there was no difference in new bone formation among the three experimental groups. In conclusion, there was no difference in new bone formation using Bio-Oss®, Cerasorb®, and a mixture of Bio-Oss® and Cerasorb®.

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

scholarly journals Effectiveness of Platelet-Rich Fibrin as an Adjunctive Material to Bone Graft in Maxillary Sinus Augmentation: A Meta-Analysis of Randomized Controlled Trails

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Ruimin Liu ◽  
Mingdong Yan ◽  
Sulin Chen ◽  
Wenxiu Huang ◽  
Dong Wu ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Formation ◽  
Bone Graft ◽  
Bone Substitute ◽  
Meta Analysis ◽  
New Bone Formation ◽  
Sinus Augmentation ◽  
Platelet Rich Fibrin ◽  
Randomized Controlled ◽  
Tissue Area

Purpose. To date, it remains unknown whether the addition of platelet-rich fibrin (PRF) to bone grafts actually improves the effectiveness of maxillary sinus augmentation. This study aimed to perform a meta-analysis to evaluate the efficacy of PRF in sinus lift.Materials and Methods. PubMed, Embase, and the Cochrane Library were searched. Randomized controlled studies were identified. The risk of bias was evaluated using the Cochrane Collaboration tool.Results. Five RCTs were included in our meta-analysis. Clinical, radiographic, and histomorphometric outcomes were considered. No implant failure or graft failure was detected in all included studies within the follow-up period. The percentage of contact length between newly formed bone substitute and bone in the PRF group was lower but lacked statistical significance (3.90%, 95% CI, -2.91% to 10.71%). The percentages of new bone formation (-1.59%, 95% CI, -5.36% to 2.18%) and soft-tissue area (-3.73%, 95% CI, -10.11% to 2.66%) were higher in the PRF group but were not significantly different. The percentage of residual bone graft was not significant in either group (4.57%, 95% CI, 0% to 9.14%).Conclusions. Within the limitations of this review, it was concluded that there were no statistical differences in survival rate, new bone formation, contact between newly formed bone and bone substitute, percentage of residual bone graft (BSV/TV), and soft-tissue area between the non-PRF and PRF groups. Current evidence supporting the necessity of adding PRF to bone graft in sinus augmentation is limited.

Influence of Homemade and Commercial Fibrin Sealant on Alveolar Bone Healing - an Experimental Study.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4110-4110
Author(s):  
Maria Elvira P. Correa ◽  
Oslei P. Almeida ◽  
Danyel Peres ◽  
Marcelo C. Alves ◽  
Fernando F. Costa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Healing ◽  
Fibrin Sealant ◽  
Factor Xiii ◽  
Alveolar Bone ◽  
Healing Process ◽  
Control Group ◽  
Image Analysis System ◽  
New Bone Formation ◽  
Fibrin Sealants

Abstract The beneficial aspects of fibrin sealants for soft tissues are well documented, but studies of their direct influence on bone healing and their effectiveness in augmenting bone graft healing have produced conflicting results. The aim of this study was to evaluate the influence of fibrin sealants (FS) in the alveolar bone healing process during a period of up to 28 days. Seventy-five Wistar rats were submitted to a superior incisor extraction after intramuscular anesthesia (kentamine chloridrate 10% – 10mg/kg and tiazina chloridrate-5mg/kg). The rats were divided into tree different groups and the sealants were introduced into the alveolar bone. The first group (25) received human homemade fibrin glue, the second group (25) FS associated to factor XIII and aprotinin (Beriplast; Aventis-Beringher) and the third group (25) was the control. Animals were sacrificed by prolonged diethyl inhalation on days 7, 14 and 28 after surgery. Animal craniums were dissected and submitted to a decalcification, and preparated for H&E light microscopy. The morphometric study was performed by means of an interactive computerized image analysis system KS400 (Zeiss, Jena). New bone formation was carefully delimited in four different alveolar regions (apical, two middle areas and cervical) of each specimen. The data were statistically analyzed using multiple regression, ANOVA and Tukey test. Results showed that the amount of alveolar bone formation (μm) in the control group and commercial sealant was statistically similar. However, alveolus receiving homemade sealant presented less amount of new bone formation comparing to commercial sealant and control group (p=0.0034) (figure 1). The present study demonstrated that homemade fibrin sealant delays osteogenic formation. Commercial sealant did not improve alveolar repair however, the amount of new bone formation was slightly higher comparing to the control, probably due to the factor XIII present in this sealant. Figure Figure

The Effect of Calcium Sulfate with Alloplast on the Periodontal Regeneration in One-Wall Intrabony Defect of Beagle Dogs

2008 ◽  
Vol 47-50 ◽  
pp. 213-217 ◽  
Author(s):  
Sung Bae Hong ◽  
Young Taek Kim ◽  
Kyung Joon Chai ◽  
Seong Ho Choi ◽  
Kyoo Sung Cho ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Body Fluid ◽  
Calcium Sulfate ◽  
Setting Time ◽  
Periodontal Regeneration ◽  
Control Group ◽  
Favorable Result ◽  
New Bone Formation ◽  
Natural Healing

Calcium sulfate used in this study is newly fabricated to overcome its disadvantages; brittleness, difficult handling properties, rapid resorption and reaction heating. Especially when calcium sulfate is used in the graft procedure, it is difficult to control the setting time, solubility into blood and body fluid. To improve the handling properties, Calcium sulfate is mixed with Carboxylmethylcellulose (CMS). The putty type calcium sulfate used in the present study presented lower values in cementum formation and new bone formation than the control group. This may be due to use of poor osteoinductive calcium sulfate alone in the defect, limiting new bone formation. In addition, complete resorption of calcium sulfate after 8 weeks presents that addition of polymer failed to delay the resorption rate of calcium sulfate. Rather, it seemed to disturb the natural healing of the defect when compared with the findings in the control group. But the favorable result of calcium sulfate is shown when used with alloplast. The material used in the present study was developed for easier formability and manipulation avoiding scattering or dissolving of the material with blood or body fluid. However, the results reveal that further studies on effect of calcium sulfate with various other bone graft materials and ideal ratios in mixing the two should be determined in the future.However, the results reveal that further studies on effect of calcium sulfate with various other bone graft materials and ideal ratios in mixing the two should be determined in the future.

scholarly journals Osseointegration of Maxillary Dental Implants in Diabetes Mellitus Patients: A Randomized Clinical Trial Human Histomorphometric Study

2020 ◽  
Vol 10 (19) ◽  
pp. 6762
Author(s):  
Lyly Sam ◽  
Siriporn Chattipakorn ◽  
Pathawee Khongkhunthian
Keyword(s):  
Type 2 Diabetes ◽  
Bone Formation ◽  
Dental Implants ◽  
Pearson Correlation ◽  
Titanium Implant ◽  
Control Group ◽  
Healthy Controls ◽  
New Bone Formation ◽  
Implant Surface

Background: Survival of dental implants in well-controlled Type 2 diabetes (T2DM) was found to be comparable to that in healthy patients. However, to our best knowledge, there have been no studies of the bone histomorphometry of osseointegration in patients with Type 2 diabetes. Purpose: To compare bone-implant-contact (BIC) and new bone formation between well-controlled Type 2 diabetes with HbA1c of less than 8% and healthy controls. Methods: 10 diabetic (T2DM) patients and 10 healthy controls were selected. Each patient received a 2.5 mm × 5 mm micro-implant in the maxilla, in either the premolar or first molar area. After 8 weeks of healing, the micro-implant was retrieved using a trephine bur and sent for bone histomorphometric analysis. A commercial titanium implant was immediately placed as the conventional treatment. Results: The mean BIC (30.73%) in T2DM patients was significantly lower than in the healthy patients (41.75%) (p = 0.01). New bone formation around the implant surface was reduced in T2DM patients (36.25%) compared to that in the control group (44.14%) (p = 0.028). The Pearson correlation coefficient revealed a strong correlation between increased HbA1c and decreased BIC (p < 0.05) and decreased new bone formation (p < 0.05). Conclusions: Within the limitation of this study, bone-to-implant contact and bone healing around dental implants in T2DM patients were significantly lower than in healthy patients.

Guided Tissue Regeneration of the Mixture of Human Tooth-Ash and Plaster of Paris in Dogs

2007 ◽  
Vol 330-332 ◽  
pp. 1327-1330 ◽  
Author(s):  
Ha Ra Ku ◽  
Hyun Seon Jang ◽  
S.G. Kim ◽  
Moon Jin Jeong ◽  
Joo Cheol Park ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Human Tooth ◽  
Control Group ◽  
Graft Material ◽  
Bone Graft Material ◽  
Plaster Of Paris ◽  
Experimental Group

This study evaluated the efficacy of periodontal tissue regeneration using guided tissue regeneration (GTR) with a mixture of tooth ash and plaster as a bone substitute material in the treatment of class II furcation defects in dogs. GTR was performed at the site of a surgically formed mandibular premolar bifurcation area in dogs. Four adult dogs were used in the animal study. The animals were in a good periodontal state without any systemic disease. A resorbable membrane (Bio-Gide®, Swiss) and bone graft material using a mixture of tooth ash and plaster were used to examine the biological activity. The defect in the control group was filled a Bio-Gide® only. For the experimental group, the defects were filled with a mixture of tooth ash and plaster and covered by a Bio-Gide®. Oral rinsing with 0.12% chlorhexidine was performed twice a day for 2 weeks. In control group, new bone formation was observed adjacent to the preexisting bone. Soft tissue ingrowth was also noted. In experimental group, new lamellar type trabecular bone formation as well as centum and PDL regeneration was observed in the base of the infrabony pocket. More bone regeneration would be expected when GTR is performed using a mixture of human tooth-ash and plaster of Paris as a bone graft material.

Direct Current Electric Stimulation in Implant Osseointegration: An Experimental Animal Study With Sheep

2013 ◽  
Vol 39 (6) ◽  
pp. 671-679 ◽  
Author(s):  
Guhan Dergin ◽  
Mustafa Akta ◽  
Bahar Gürsoy ◽  
Yalçin Devecioglu ◽  
Mehmet Kürkçü ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dental Implants ◽  
Direct Current ◽  
Electric Stimulation ◽  
Bone Fractures ◽  
Late Phase ◽  
Biochemical Properties ◽  
Control Group ◽  
New Bone Formation ◽  
Significant Difference

In an effort to obtain a high-quality bone-implant interface, several methods involving alteration of surface morphological, physicochemical, and biochemical properties are being investigated. The aim of our study was to increase the osseointegration rate and quality and decrease the waiting period of dental implants before loading by using a microelectric implant stimulator device. It imitates microelectrical signals, which occur in bone fractures described in terms of piezoelectric theory. A single dental implant (Zimmer Dental), 3.7 mm in diameter, was inserted into the tibia of sheep bilaterally. Twenty-four dental implants were inserted into 12 sheep. Implant on the tibia of each sheep was stimulated with 7.5 μA direct current (DC), while the other side did not receive any stimulation and served as a control. Animals were sacrificed 1, 2, and 3 months after implantation. Bone segments with implants were processed with unclassified method. The determination of new bone formation and osseointegration around the dental implants was investigated by means of undecalcified method, histomorphologically. No statistically significant difference in bone-to-implant contact (BIC) ratio, osteoblastic activity, and new bone formation was found between the stimulation group and the control group at the late phase of healing (4, 8, and 12 weeks). No evidence was found that electric stimulation with implanted 7.5 μA DC is effective at late phase implant osseointegration on a sheep experimental model.

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