scholarly journals Comparison of antioxidative activity data for aqueous extracts of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), thyme (Thymus vulgaris L.), and agrimony (Agrimonia eupatoria L.) obtained by conventional methods and the DNA-based biosensor

2011 ◽  
Vol 21 (No. 2) ◽  
pp. 78-84 ◽  
Author(s):  
Ľ. Heilerová ◽  
M. Bučková ◽  
P. Tarapčík ◽  
S. Šilhár ◽  
J. Labuda

The antioxidative properties of aqueous plant extracts were evaluated using common methods such as the Rancimat and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical method. Moreover, a voltammetric procedure based on the protective effect of antioxidants against the oxidative DNA damage was employed using a disposable DNA biosensor fabricated as a screen-printed electrode chemically modified by calf thymus double stranded (ds) DNA. The total polyphenols were also determined spectrophotometrically with the Folin-Ciocalteu agent. The extracts of oregano and lemon balm exhibited significantly higher activity than those of thyme and agrimony. The results were treated statistically and their operational character is discussed.  

Author(s):  
Daniela Sandru ◽  
Violeta Niculescu ◽  
Ecaterina Lengyel ◽  
Ovidiu Tița

This meaning of this specific work is to identify and quantify the polyphenolic compounds that exist in plants with bioactive potentially. The study was monitorising 16 different plants: bilberry (Vaccinium myrtillus), artichoke (Cynara scolymus), chicory (Cichorium intybus), dumb (Teucrium chamaedrys), fennel (Foeniculum vulgare), thorn (Xanthium spinosum), juniper (Juniperus communis), mint (Mentha), cranberry (Vaccinium vitis-idaea), hawthorn (Crataegus monogyna), wormwood (Artemisia absinthium), willow herb (Epilobium), lemon balm (Melissa officinalis), St. John's wort (Hypericum perforatum), oregano (Origanum vulgare), centaury (Centaurium erythraea). The total polyphenolic compound was determined onspectrophotometricmethod,Folin-Ciocalteu. The polyphenols have a very wide range value starting on low amounts on centaury (Centaurium erythraea) 271.613 mg/L and reaching highest values of 5975.616 mg/L in wormwood (Artemisia absinthium). The results can be use in the design of digestive drinks in the food industry due to higher concentration of total polyphenols in the studied plants.


2000 ◽  
Vol 6 (3) ◽  
Author(s):  
É. Németh ◽  
G. Székely

Several species in the Lamiaceae family are therapeutically significant medicinal plants. Such as sage (Salvia officinalis L.), rosemary (Rosmarinus officinalis L.), peppermint (Moldier piperita L.). marjoram (Majomna hortensis Winch), thyme (Thymus vulgaris L.), Origanum spp., hyssop (Hyssopus officinalis L.), Lavandula spp., basil (Ocimum basalicum L.), lemon-balm (Melissa officinalis L.) are regularly produced in Hungary. Nevertheless, data on their floral biology are not satisfactory. This review intends to gather information on the characteristic constitution of flowers and inflorescences, blooming dynamics, pollination mechanism and crossability of some of the most significant medicinal and spice species Flowers are mainly hermaphrodite, however, also monoeceous ones are turning up: pistillate flowers marjoram, tyme, oregano. In flowering of a plant individual, a basipetal blooming sequence is described. These species have a floral structure fully adapted to pollination by insects, and the majority of them is an excellent honey-plant (lavandel, hyssop, sage, etc.). Flowers exhibit characteristic proterandry, therefore autogamy is considered to be almost impossible, and geintonogamy as well as xenogamy to be the most characteristic ways of fertilization. However, in several cases self-fertilization proved to be also successful. Crossability among cultivars or species is depending on the genotype combination and usually produces less seed than the above forms of fertilization.  


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 633e-634
Author(s):  
J.R. Schroeder ◽  
Alice Le Duc

Ten culinary and ornamental herbs were evaluated for time and quality of rooting of tip cuttings. The taxa included in the study were oregano (Origanum vulgare), lemon thyme (Thymus ×citriodorata), applemint (Mentha suavolens), Persian catnip (Nepeta ×faassenii), lemon balm (Melissa officinalis), southernwood (Artemisia abrotanum), caraway thyme (Thymus herba-barona), hyssop (Hyssopus officinalis), `Blue Wonder' catnip (Nepeta `Blue Wonder'), pineapplemint (Mentha suavolens var. variegata). Four replicates of each species were used. The cuttings, untreated and rooting hormone treated, were placed under intermittent mist, then cuttings potted when a 1- to 1.5-inch root ball had developed. Most of the stock suffered from some chlorosis during rooting; southernwood cuttings particularly displayed severe chlorosis which was overcome with 2 weeks of constant-feed fertilizer after potting. Oregano displayed the best results, rooting in seven days with or without treatment. It produced a sellable 4-inch pot in 31 days from sticking the cuttings. Lemon thyme, applemint, Persian catnip, and lemon balm all rooted in 14 days if treated. No difference was observed in days to rooting between treated and untreated lemon thyme. Untreated cuttings of lemon balm, applemint, and Persian catnip rooted in 25 to 30 days. Treated applemint cuttings not only rooted more quickly but produced a marketable 4-inch pot in significantly less time. Southernwood and caraway thyme rooted in 25 days, with no significant difference between treated and untreated cuttings. Hyssop, pineapplemint, and `Blue Wonder' catnip took about 30 days, also with no significant difference between treated and untreated cuttings.


2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Cortéz Raymundo

La carne de conejo es rica en vitaminas, se puede integrar perfectamente a una dieta saludable, sin embargo el factor que influye en su consumo es su sabor y olor; muchas personas catalogan su aroma como fuerte, lo que resulta desagradable. Para mejorar sabor y apariencia de la carne de conejo, se trabajó con un grupo de 25 conejos de las razas Holandés y Californiano de aproximadamente 3 meses de edad y un peso de 2 kg, el agua de bebida fue a base de una infusión utilizando 10 g/2 l de agua hirviendo, siendo estas plantas aromáticas Cilantro (Coriandrumsativum), Hierbabuena (Mentha spicata linnaeus), Orégano (Origanum vulgare) y Tomillo (Thymus vulgaris); como alimentación base a todos los animales se les administro alimento balanceado comercial para conejos. Se utilizaron grupos de 5 conejos por cada una de las plantas mencionadas y un grupo testigo. Se les realizo un examen clínico al finalizar el experimento la cual no presentaron signos de enfermedad. Al termino de cuarenta días finalizado el experimentos e obtuvieron los resultados: tratamiento del cilantro con un peso de 13.60 kg, orégano 11.83 kg; hierba buena 11.40 kg, tomillo 10 kg y el tratamiento testigo 10 kg. Se sacrificaron los conejos, se coció la carne únicamente “al vapor” (165oC), en un tiempo de 50 minutos. Para comprobar si el experimento funciono se invitaron a diez catadores para que calificaran directamente si la carne tenía algún sabor especifico. Los datos que se obtuvieron en el panel de catadores se analizaron estadísticamente por medio de bloques al azar con el programa de Infostat. Obteniendo resultado con diferencia no significativa, (p≤0.05). Con las medias de cada tratamiento se aplicó la prueba de Tukey donde se obtuvieron los siguientes resultados: según la variable de la presentación, olor, sabor y textura la más aceptable por el panel de catadores fue el tratamiento evaluado con la hierba aromática del Tomillo. La conclusión fue que los conejos que fueron tratados con tomillo se tornaron de color blanco que es el normal a un color grisáceo, estando suave y de buena consistencia; siendo la más preferida. El cilantro no cambio su coloración ni la consistencia de la carne pero si el sabor, en cuanto a la carne tratado con orégano y hierbabuena, el cambio se observó en la terneza,se dio cambio de color amarillento pero más dura que la normal con sabor y olor agradable.


2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Mouna Souihi ◽  
Rayda Ben Ayed ◽  
Imen Trabelsi ◽  
Marwa Khammassi ◽  
Nadia Ben Brahim ◽  
...  

Lemon balm (Melissa officinalis L.) is one of the rare medicinal plants in Tunisia. It was found only in two sites in the north of Tunisia with a small number of plants. The study of germination under the NaCl and PEG effect showed that Tunisian lemon balm seeds were sensitive to saline and osmotic stress. Morphological and biochemical characterizations of Tunisian M. officinalis were performed. Results showed that the Tunisian populations presented plants with long, broad leaves and weak branching. The major constituent in leaf essential oil was germacrene-D with a percentage ranging from 29.17 to 24.6%, and the major fatty acids were polyunsaturated fatty acids, linoleic acid, ranging from 73.93 to 66.74%. The phenolic content of M. officinalis extract varied significantly among origins which could explain the high variation in antiradical scavenging activity. The evaluation of allelopathic activities showed that the extract of the lemon balm leaves presented an allelopathic effect with the majority of the tested seeds.


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