scholarly journals Qualitative detection of haptoglobin mRNA in bovine and human blood leukocytes and bovine milk somatic cells

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 515-520
Author(s):  
Thielen MA ◽  
M. Mielenz ◽  
S. Hiss ◽  
H. Sauerwein
Keyword(s):  
Mammary Gland ◽  
Somatic Cells ◽  
Bovine Milk ◽  
Bovine Mammary Gland ◽  
Blood Leukocytes ◽  
Human Leukocytes ◽  
Mrna Transcripts ◽  
Stromal Tissue ◽  
Tissue Homogenates ◽  
Milk Somatic Cells

Local expression of haptoglobin (Hp) mRNA in tissue homogenates from different sites of the bovine mammary gland has recently been established. Besides alveolar and stromal tissue, blood cells are one potential cellular location of Hp expression. The aim of this study was to investigate whether bovine leukocytes might contribute to the Hp mRNA transcripts found in the mammary gland after their migration into this organ. For this purpose RT-PCR was carried out with total RNA extracted from leukocytes in blood and from somatic cells in milk of three dairy cows. In comparison, human leukocytes of four donors were examined. Both bovine blood and milk somatic cells showed distinct signals for Hp mRNA in all samples in contrast to human leukocytes where Hp mRNA was detected in only two out of four donors after reamplification. In conclusion, the presence of Hp mRNA in blood derived leukocytes indicates that these cells contribute to the Hp mRNA transcripts found in the bovine mammary gland. It remains to be clarified whether other cells within the mammary gland are also capable of Hp mRNA synthesis.

scholarly journals Effects of an alpha‐linolenic acid enriched diet on mRNA expression of lipid metabolism genes in bovine milk somatic cells

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Katherine M Hunt ◽  
Kevin G Carnahan ◽  
Brent P Hatch ◽  
Kelleen O Parnell ◽  
Janet E Williams ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Mrna Expression ◽  
Linolenic Acid ◽  
Somatic Cells ◽  
Bovine Milk ◽  
Alpha Linolenic Acid ◽  
Milk Somatic Cells ◽  
Lipid Metabolism Genes

445 Toxy-Nil and Unike Plus modulate differences in gene expression of milk somatic cells isolated from mammary gland of lactating dairy cows fed aflatoxin B1

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 218-219
Author(s):  
R. O. Rodrigues ◽  
R. O. Rodrigues ◽  
D. R. Ledoux ◽  
G. E. Rottinghaus ◽  
R. Borutova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Aflatoxin B1 ◽  
Somatic Cells ◽  
Lactating Dairy Cows ◽  
Milk Somatic Cells

scholarly journals The expression of cytokines in the milk somatic cells, blood leukocytes and serum of goats infected with small ruminant lentivirus

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Justyna Jarczak ◽  
Danuta Słoniewska ◽  
Jarosław Kaba ◽  
Emilia Bagnicka
Keyword(s):  
Protein Expression ◽  
Small Ruminant ◽  
Somatic Cells ◽  
Immunological Response ◽  
Dairy Goats ◽  
Blood Leukocytes ◽  
Protein Levels ◽  
Tnf Α ◽  
Gene And Protein Expression ◽  
Milk Somatic Cells

Abstract Background The present study aimed to determine the expression of cytokines, which is associated with the immunological response of dairy goats against small ruminant lentivirus (SRLV). The study was conducted on 26 dairy goats in their second to sixth lactation, which were divided by breed and parity into two groups: SRLV naturally infected (N = 13) and non-infected (N = 13) animals. All goats in the study were asymptomatic. The milk and blood samples, which served as studied material were taken on days 7, 30, 120 and 240 of the lactation. The gene and protein expression of several cytokines was studied using Real-Time PCR and ELISA methods. Results INF-β and INF-γ expression was down-regulated in the milk somatic cells (MSC) of SRLV-infected goats. However, an increased concentration of INF-β was observed in the MSC in SRLV-infected goats, while INF-γ expression was not observed in both SRLV-infected and non-infected animals The SRLV-infected goats also displayed decreased expression of IL-1α, IL-1β, IL-6 and INF-γ genes in the blood leukocytes,with IL-1α, IL-1β and IL-6 protein levels also being decreased in the sera. TNF-α was the only gene that demonstrated increased expression in both the MSC and the blood of infected animals; however, no such overexpression was observed at the protein level. Conclusions SRLV probably influences the immune system of infected animals by deregulating of the expression of cytokines. Further, epigenetic studies may clarify the mechanisms by which SRLV regulates the gene and protein expression of the host.

scholarly journals MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland

2003 ◽  
Vol 71 (9) ◽  
pp. 4842-4849 ◽  
Author(s):  
Amanda J. Smith ◽  
Philip N. Ward ◽  
Terence R. Field ◽  
Catherine L. Jones ◽  
Ruth A. Lincoln ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Inflammatory Response ◽  
Metal Binding ◽  
Parental Strain ◽  
Insertional Mutagenesis ◽  
Bovine Milk ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis ◽  
Efficient Uptake ◽  
Single Insertion

ABSTRACT A mutant strain of Streptococcus uberis (AJS001) that was unable to grow in bovine milk was isolated following random insertional mutagenesis. The level of growth in milk was restored to that of the parental strain (strain 0140J) following addition of MnSO4 but not following addition of other metal ions. The mutant contained a single insertion within mtuA, a homologue of mtsA and psaA, which encode metal-binding proteins in Streptococcus pyogenes and Streptococcus pneumoniae, respectively. Strain AJS001 was unable to infect any of eight quarters on four dairy cows following intramammary challenge with 105 CFU. Bacteria were never recovered directly from milk of these animals but were detected following enrichment in Todd-Hewitt broth in three of eight milk samples obtained within 24 h of challenge. The animals showed no inflammatory response and no signs of mastitis. Three mammary quarters on two different animals simultaneously challenged with 600 CFU of the parental strain, strain 0140J, became colonized, shed high numbers of S. uberis organisms in milk, displayed a marked inflammatory response to infection, and showed overt signs of mastitis. These data indicate that mtuA was required for efficient uptake of Mn2+ during growth in bovine milk and infection of the lactating bovine mammary gland.

Evaluation of milk somatic cells as a source of mRNA for study of lipogenesis in the mammary gland of lactating beef cows supplemented with dietary high-linoleate safflower seeds1

2006 ◽  
Vol 84 (9) ◽  
pp. 2399-2405 ◽  
Author(s):  
C. M. Murrieta ◽  
B. W. Hess ◽  
E. J. Scholljegerdes ◽  
T. E. Engle ◽  
K. L. Hossner ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Somatic Cells ◽  
Beef Cows ◽  
Milk Somatic Cells

A robust segmentation method for counting bovine milk somatic cells in microscope slide images

2015 ◽  
Vol 115 ◽  
pp. 142-149 ◽  
Author(s):  
G.J.A. de Melo ◽  
V. Gomes ◽  
C.C. Baccili ◽  
L.A.L. de Almeida ◽  
A.C. de C. Lima
Keyword(s):  
Somatic Cells ◽  
Bovine Milk ◽  
Segmentation Method ◽  
Microscope Slide ◽  
Robust Segmentation ◽  
Milk Somatic Cells

scholarly journals Defense mechanisms in the bovine mammary gland

2017 ◽  
Vol 55 (3) ◽  
pp. 235 ◽  
Author(s):  
V. S. MAVROGIANNI (Β. Σ. ΜΑΥΡΟΓΙΑΝΝΗ) ◽  
G. C. FTHENAKIS (Γ. Χ. ΦΘΕΝΑΚΗΣ)
Keyword(s):  
Mammary Gland ◽  
Defense Mechanisms ◽  
Somatic Cells ◽  
Bovine Mammary Gland ◽  
Defence Mechanisms ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Cellular Defence ◽  
Stimulating Factor

In the present article the literature on the defence mechanisms of the bovine mammary gland is reviewed. The article is divided into three sections: (i) The teat, (ii) Cellular defence mechanisms (number of somatic cells, type of somatic cells, counting of somatic cells, defence role of somatic cells: macrophages, neutrophils, lymphocytes) and (iii) Chemoral defence mechanisms (cytokines: IL-1, IL-2, IL-8, granulocyte/macrophage-colony stimulating factor and TNF-a, immunoglobulins, complement, lactoferrin, lactoperoxidase/thiocyanate/ H 2 0 2 system, lysozyme).

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